EP0892783A1 - Derives d'acides carboxyliques a effet inhibant l'agregation - Google Patents

Derives d'acides carboxyliques a effet inhibant l'agregation

Info

Publication number
EP0892783A1
EP0892783A1 EP97918113A EP97918113A EP0892783A1 EP 0892783 A1 EP0892783 A1 EP 0892783A1 EP 97918113 A EP97918113 A EP 97918113A EP 97918113 A EP97918113 A EP 97918113A EP 0892783 A1 EP0892783 A1 EP 0892783A1
Authority
EP
European Patent Office
Prior art keywords
group
alkyl
phenyl
piperidin
general formula
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP97918113A
Other languages
German (de)
English (en)
Inventor
Helmut Pieper
Günter Linz
Volkhard Austel
Frank Himmelsbach
Brian Guth
Johannes Weisenberger
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Boehringer Ingelheim Pharma GmbH and Co KG
Original Assignee
Dr Karl Thomae GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dr Karl Thomae GmbH filed Critical Dr Karl Thomae GmbH
Publication of EP0892783A1 publication Critical patent/EP0892783A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D211/00—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings
    • C07D211/04—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom
    • C07D211/06—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members
    • C07D211/36—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D211/40—Oxygen atoms
    • C07D211/44—Oxygen atoms attached in position 4
    • C07D211/46—Oxygen atoms attached in position 4 having a hydrogen atom as the second substituent in position 4
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D211/00—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings
    • C07D211/04—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom
    • C07D211/06—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members
    • C07D211/08—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hydrocarbon or substituted hydrocarbon radicals directly attached to ring carbon atoms
    • C07D211/18—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hydrocarbon or substituted hydrocarbon radicals directly attached to ring carbon atoms with substituted hydrocarbon radicals attached to ring carbon atoms
    • C07D211/20—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hydrocarbon or substituted hydrocarbon radicals directly attached to ring carbon atoms with substituted hydrocarbon radicals attached to ring carbon atoms with hydrocarbon radicals, substituted by singly bound oxygen or sulphur atoms
    • C07D211/22—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hydrocarbon or substituted hydrocarbon radicals directly attached to ring carbon atoms with substituted hydrocarbon radicals attached to ring carbon atoms with hydrocarbon radicals, substituted by singly bound oxygen or sulphur atoms by oxygen atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D211/00—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings
    • C07D211/04—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom
    • C07D211/06—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members
    • C07D211/08—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hydrocarbon or substituted hydrocarbon radicals directly attached to ring carbon atoms
    • C07D211/18—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hydrocarbon or substituted hydrocarbon radicals directly attached to ring carbon atoms with substituted hydrocarbon radicals attached to ring carbon atoms
    • C07D211/26—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hydrocarbon or substituted hydrocarbon radicals directly attached to ring carbon atoms with substituted hydrocarbon radicals attached to ring carbon atoms with hydrocarbon radicals, substituted by nitrogen atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D211/00—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings
    • C07D211/04—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom
    • C07D211/06—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members
    • C07D211/36—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D211/56—Nitrogen atoms
    • C07D211/58—Nitrogen atoms attached in position 4

Definitions

  • the present invention relates to carboxylic acid derivatives of the general formula
  • R a is a hydrogen atom, a C-j__5-alkyl or phenyl-C ⁇ __3-alkyl group, in each of which the alkyl part is replaced by a carboxy-, C- ] __3-alkoxycarbonyl, aminocarbonyl, NC- j __ 3 -alkylamino- carbonyl, N, N-di- (C ⁇ ._3-alkyl) aminocarbonyl, vinyl or ethynyl group or, if the above-mentioned substituents are not on an ⁇ -carbon atom adjacent to a nitrogen atom, by a hydroxy, C- L _ 3 -alkoxy-, amino-, C-j__3-alkylamino or di- (C-j__3-alkyl) amino group, or a residue which can be split off in vivo, R] -, and R c , which may be the same or different, each have a hydrogen
  • R-j_ is a hydrogen atom, a C- j ⁇ -alkyl, phenyl-C ⁇ _3-alkyl or phenyl group,
  • R 2 is a hydrogen atom, a C-j__3 alkyl or phenyl C-_ 3 alkyl group and
  • R 3 represents a hydrogen atom, a C 1 _ 3 -alkyl, phenyl-C- j __ 3 -alkyl, C-L_3-alkylcarbonyl or C- ] __5-alkyl ⁇ ulfonyl group,
  • B is a 3-piperidinylene, 4-piperidinylene or 1,4-piperazinylene group, in each of which a methylene group adjacent to a nitrogen atom can be replaced by a carbonyl group, a 1,4-piperazinylene group additionally being represented by R 1 and R 2 c can be substituted and Rj and R c are defined as mentioned above, a phenylene, cyclohexylene, pyridinylene, pyridazinylene, pyrimidinylene or pyrazinylene group,
  • D a -OR 1 CR 4 -CO-, -NI ⁇ -HCR-L-CO-, -NR 3 -CH 2 CH 2 C0-, -CH 2 CO-, -CHR ⁇ CH 2 CO- or (-0-) 2 CH-C0 group in which R- j _ and R3 are defined as mentioned above and R 4 is a hydrogen atom, a C-j__3-alkyl-, hydroxy- C 1 _3-alkyl-, carboxy-C ⁇ _ 3 -alkyl-, Cj_.3-alkoxycarbonyl- c l -3 "represents alkyl, C3_7-cycloalkoxycarbonyl-Ci_3-alkyl, phenyl-C ⁇ _3-alkyl, phenyl, pyridyl-C * j__3-alkyl or pyridyl group,
  • E is a hydroxyl group, an alkoxy group with 1 to 6 carbon atoms, a phenylalkoxy group in which the alkoxy part can contain 1 to 3 carbon atoms, a cycloalkoxy group with 3 to 9 carbon atoms in which the cycloalkyl part with 5 to 8 carbon atoms can additionally be substituted by one or two alkyl groups each having 1 to 3 carbon atoms, a cycloalkoxy group having 5 to 8 carbon atoms, in which in the cycloalkyl part a methylene group in the 3- or 4-position by an oxygen atom or by one optionally by a Alkyl, phenylalkyl or phenylalkoxycarbonyl group in which the alkyl and alkoxy part can each contain 1 to 3 carbon atoms, or is replaced by an alkanoyl group having 2 to 6 carbon atoms and the imino group substituted and the cycloalkyl part additionally by or two alkyl groups each having 1
  • Rg represents a hydrogen atom or a C-j__g alkyl group and R 7 represents a C ⁇ 5-alkyl, C-j__5-alkoxy, C 5 _7-cycloalkyl or C5_7-cycloalkoxy group,
  • E is an ⁇ -amino group of a natural D- or L-amino acid and its ester.
  • a phenyl group or "a phenylene group” is in each case in particular one optionally by fluorine, chlorine, bromine or iodine atoms, by C 1 -C 3 -alkyl, trifluoromethyl or nitro -, Amino-, C-j_.3-alkylamino-, di- (C - * __ 3-alkyl) -amino-, C 1 _4-alkanoylamino-, hydroxy-, C -] __ 3-alkoxy-, carboxy-, C ⁇ _3 -Alkoxycarbonyl-, C3_7-cycloalkoxycarbonylalkoxy-, hydroxycarbonyl-C - * __ 3-alkoxy-, C -] __ 3-alkoxycarbonyl-C- j __3-alk-oxy-, aminocarbonyl-, C ⁇ __3-alkyl, aminocarbonyl-, C ⁇ __3
  • esters of a natural ⁇ -amino acid whose C- ] __g_alkyl-, C 2 _g-alkenyl-, C5_7-cycloalkyl-, phenyl- or phenyl-C ⁇ _3-alkyl esters such as methyl, ethyl, n-propyl, isopropyl , tert-butyl, allyl, phenyl or benzyl ester,
  • alkanoyl group with a total of 1 to 6 carbon atoms a benzoyl, allyloxycarbonyl, C ⁇ _5alkoxycarbonyl or phenyl-C; -__ 3-alkoxycarbonyl group such as the formyl, acetyl, propionyl, Butanoyl, pentanoyl, hexanoyl, benzoyl, allyloxycarbonyl, Methoxycarbonyl, ethoxycarbonyl, propoxycarbonyl, isopropoxycarbonyl, butoxycarbonyl, tert.
  • pen-toxoxycarbonyl, hexoxycarbonyl, benzyloxycarbonyl, phenylethoxycarbonyl or phenylpropoxycarbonyl group isopropoxycarbonyl, butoxycarbonyl, tert.
  • R a is a hydrogen atom, a or phenyl-C - * __ 3-alkyl group, in each of which the alkyl part is replaced by a carboxy-, C ] __ 3 -alkoxycarbonyl-, aminocarbonyl-, NC-j__ 3 -alkylamino-carbonyl-, N, N-di- ( C - * __ 3-alkyl) aminocarbonyl, vinyl or ethynyl group or, if the abovementioned substituents are not on an ⁇ -carbon atom adjacent to a nitrogen atom, by a hydroxy, C - * __ 3-alkoxy or amino -, C -] __ 3-alkylamino or di- (C ⁇ _3-alkyl) amino group, or a residue which can be split off in vivo,
  • Rj- and R c which may be the same or different, each represent a hydrogen atom or the side chain of a natural D- or L- ⁇ -amino acid and its esters and ethers,
  • R 1 is a hydrogen atom, a C 1 _3 * alkyl, phenyl-Ci.3-alkyl or phenyl group,
  • R 2 is a hydrogen atom, a C-t__3-alkyl or phenyl-C- j __3-alkyl group and
  • R3 represents a hydrogen atom, a C ⁇ _3-alkyl, phenyl-C ⁇ _3-alkyl, C ⁇ _3-alkylcarbonyl or C ⁇ _5-alkylsulfonyl group, X ⁇ _, X 2 and X 3 , which may be the same or different, each a nitrogen atom or a methine group, in addition in the heterocyclic rings mentioned above in which X 2 or X3 or X 2 and X3 each represent a nitrogen atom a methylene group linked to a ring nitrogen atom can be replaced by a carbonyl group,
  • B is a 3-piperidinylene, 4-piperidinylene or 1,4-piperazinylene group, in each of which a methylene group adjacent to a nitrogen atom can be replaced by a carbonyl group, a 1,4-piperazinylene group additionally being substituted by R-- ) and R c can be substituted and R ⁇ and R c are defined as mentioned above, a phenylene, cyclohexylene or pyridazinylene group,
  • R l and R3 are as defined above and R 4 is a hydrogen atom, a C ⁇ _3 * alkyl, hydroxy C ⁇ _3-alkyl, carboxy-C ⁇ _3-alkyl, Ci. 3 -alkoxycarbonyl- C * 3 * -alkyl-, C 3 _ 7 -cycloalkoxycarbonyl-C ⁇ _ 3 -alkyl-, phenyl-C x _3-alkyl, phenyl, pyridyl-C ⁇ _ 3 -alkyl or pyridyl group,
  • E is a hydroxyl, C 6 alkoxy, C 3 _9 cycloalkoxy, phenyl C 3 alkoxy or R7-CO-O- (R 5 CRg) -O group, in which
  • R 5 is a hydrogen atom, a C ⁇ _g-alkyl, C3_ 7 -cycloalkyl or phenyl group,
  • Rg represents a hydrogen atom or a C ⁇ _g alkyl group and R 7 represents a C ⁇ _ 5 alkyl, C x _5 alkoxy, C 5 _ 7 cycloalkyl or C5_ 7 cycloalkoxy group,
  • a phenyl group or "a phenylene group” is in each case one in particular by fluorine, chlorine, bromine or iodine atoms, by C ⁇ _ 3 alkyl, trifluoromethyl, nitro, amino, C ⁇ _ 3 alkylamino, di (C ⁇ _3-alkyl) amino, C ⁇ _ 4 alkanoylamino , Hydroxy-, C ⁇ _3-alkoxy-, carboxy-, C ⁇ _3-alkoxycarbonyl-, C3_7-Cycloalkoxycarbon- nylalkoxy-, hydroxycarbonyl-C ⁇ _ 3 -alkoxy-, C ⁇ _ 3 -alkoxycarbonyl • C ⁇ _ 3 -alkoxy-, aminocarbonyl-, C ⁇ _3- Alkylaminocarbonyl or di- (C 1-3 alkyl) -aminocarbonyl groups mono-, di- or
  • esters of a natural ⁇ -amino acid whose C ⁇ _g-alkyl, C 2 -g-alkenyl, C 5 _ 7 cycloalkyl, phenyl or phenyl C ⁇ _ 3 alkyl esters such as the methyl, ethyl, n Propyl, isopropyl, tert.butyl, allyl, phenyl or benyl ester,
  • an alkanoyl group with a total of 1 to 6 carbon atoms a benzoyl, allyloxycarbonyl, C ⁇ _5-alkoxycarbonyl or phenyl-C ⁇ _3-alkoxy ⁇ carbonyl group such as the formyl, acetyl, propionyl, butanoyl -, Pentanoyl, hexanoyl, benzoyl, allyloxycarbonyl, methoxycarbonyl, ethoxycarbonyl, propoxycarbonyl, isopropoxycarbonyl, butoxycarbonyl, tert. Butoxycarbonyl, pen-toxoxycarbonyl, hexoxycarbonyl, benzyloxycarbonyl, phenylethoxycarbonyl or phenylpropoxycarbonyl group is to be understood,
  • Particularly preferred compounds of the above general formula I are those in which R a is a hydrogen atom, a C ⁇ _3-alkyl, phenyl-C ⁇ _3-alkyl, C ⁇ _5-alkoxycarbonyl or phenyl-C ⁇ _ 3 alkoxycarbonyl group,
  • Rj- and R c which may be the same or different, each represent a hydrogen atom or the side chain of a natural D- or L- ⁇ -amino acid and its esters and ethers,
  • A is a -CH 2 CH 2 -, -CO-CH 2 -, -CH 2 -CO-, -CH 2 -NR 3 -, -NR 3 -CH2-, -NH-CO-, -O-CO- or -CH2 "0 group in which
  • R3 represents a hydrogen atom, a C 3 alkyl, phenyl C 3 alkyl, C 3 alkylcarbonyl or C 5 alkyl sulfonyl group,
  • B is a 4-piperidinylene group or a 1,4-piperazinylene group in which a methylene group adjacent to a nitrogen atom can be replaced by a carbonyl group, the 1,4-piperazinylene groups mentioned above additionally being replaced by a carboxymethyl or C ⁇ _5 Alkoxycarbonyl group, a 1,3- or 1,4-phenylene group optionally substituted by an E-CO-CH 2 group, where E is as defined below, a 1,4-cyclohexylene or 2,5-pyridazinylene group ,
  • R3 is defined as mentioned above,
  • R l is a hydrogen atom or a C ⁇ _3 alkyl group and R 4 represents a hydrogen atom, a C 3 alkyl, hydroxy C 2 alkyl, carboxymethyl, benzyl, chlorobenzyl or phenyl group,
  • E represents a hydroxy, Ci_g-alkoxy, C3_9-cycloalkoxy or phenyl-Ci_3-alkoxy group
  • esters of a natural ⁇ -amino acid whose C ⁇ _g alkyl, C 2 _6 alkenyl, C 5 _ 7 cycloalkyl, phenyl or phenyl C ⁇ _ 3 alkyl such as methyl -, ethyl, n-propyl, isopropyl, tert-butyl, allyl, phenyl or benzyl ester and
  • R a is a hydrogen atom, a benzyl, C ⁇ _5-alkoxycarbonyl or benzyloxycarbonyl group,
  • R D and R c which may be the same or different, each represent a hydrogen atom or the side chain of a natural D or L- ⁇ -amino acid and its esters and ethers,
  • A is a -CH 2 CH 2 -, -CO-CH 2 -, -CH 2 -CO-, -CH 2 -NR 3 -, -NR 3 -CH 2 - or -NH-CO group, in which
  • R3 represents a hydrogen atom, a methyl, benzyl, acetyl or n-butylsulfonyl group, X 1 , X 2 and X 3 , which may be the same or different, each have a nitrogen atom or a methine group, with in the heterocyclic rings mentioned above in which X 2 or X 3 or X 2 and X3 each represent a nitrogen atom ⁇ additionally a methylene group linked to a ring nitrogen atom can be replaced by a carbonyl group,
  • B is a 4-piperidinylene group or a 1,4-piperazinylene group, in which a methylene group adjacent to a nitrogen atom can be replaced by a carbonyl group, the 1,4-piperazinylene groups mentioned above additionally being replaced by a carboxymethyl or C ⁇ _5 Alkoxycarbonyl group, a 1,3- or 1,4-phenylene group optionally substituted by an E-CO-CH 2 group, where E is as defined below, a 1,4-cyclohexylene or 2,5-pyridazinylene group ,
  • R3 is defined as mentioned above and R 1 represents a hydrogen atom, methyl, 2-hydroxyethyl, carboxymethyl, benzyl, chlorobenzyl or phenyl group,
  • E is a hydroxy, C ⁇ _g-alkoxy, C 3 _9-cycloalkoxy or phenyl-Ci_3-alkoxy group,
  • esters of a natural ⁇ -amino acid mentioned in the definition of the above their C ⁇ _-alkyl, C 2 _g-alkenyl, C5_7-cycloalkyl, phenyl or phenyl-Ci.3- alkyl esters such as the methyl, ethyl , n-propyl, isopropyl, tert-butyl, allyl, phenyl or benzyl ester and
  • R a ' has the meanings mentioned for R a and E' denotes a group which can be converted into a hydroxyl group by hydrolysis, treatment with an acid or base, thermolysis or hydrogenolysis,
  • R a is defined as above and E represents a hydroxyl group or E with the exception of the hydroxyl and R 7 -CO-O- (R 5 CRg) -O group as ein ⁇ is initially defined and R a represents a hydrogen atom.
  • protective groups for a hydroxyl group of a carboxyl group for example the functional derivatives of a carbo- oxy groups such as their unsubstituted or substituted amides, esters, thioesters, trimethylsilyl esters, orthoesters or iminoesters by means of hydrolysis into a carboxyl group,
  • Esters with tertiary alcohols e.g. the tert. Butyl ester, by means of treatment with an acid or thermolysis in a carboxy group and
  • Esters with aralkanols e.g. the benzyl ester can be converted into a carboxyl group by means of hydrogeolysis.
  • the hydrolysis is expediently carried out either in the presence of an acid such as hydrochloric acid, sulfuric acid, phosphoric acid, acetic acid, trichloroacetic acid, trifluoroacetic acid or mixtures thereof or in the presence of a base such as lithium hydroxide, sodium hydroxide or potassium hydroxide in a suitable solvent such as water, Water / methanol, water / ethanol, water / isopropanol, methanol, ethanol, water / tetrahydrofuran or water / dioxane at temperatures between -10 and 120 ° C, for example at temperatures between room temperature and the boiling point of the reaction mixture.
  • an acid such as hydrochloric acid, sulfuric acid, phosphoric acid, acetic acid, trichloroacetic acid, trifluoroacetic acid or mixtures thereof
  • a base such as lithium hydroxide, sodium hydroxide or potassium hydroxide
  • a suitable solvent such as water, Water / methanol, water
  • any N-acylamino or C 5 alkoxycarbonylamino groups present such as an N-trifluoroacetylamino or tert.butyloxycarbonylamino group, can be converted into the corresponding amino groups.
  • E 'in a compound of the general formula II means, for example, the tert-butyloxy group and / or R a ' the tert-butyloxycarbonyl group
  • these groups can also be treated with an acid such as trifluoroacetic acid, formic acid, p-toluenesulfonic acid , Sulfuric acid, hydrochloric acid, phosphoric acid or polyphosphoric acid, optionally in an inert solvent such as methylene chloride, chloroform, benzene, toluene, diethyl ether, tetrahydrofuran or dioxane, preferably at temperatures between -10 and 120 ° C, for example at temperatures between see 0 and 60 ° C, or thermally optionally in an inert solvent such as methylene chloride, chloroform, benzene, toluene, tetrahydrofuran or dioxane and preferably in the presence of a catalytic amount of an
  • E 'in a compound of formula II means, for example, the benzyloxy group and / or R a ' the benzyl group
  • these groups can also be hydrogenolytically in the presence of a hydrogenation catalyst such as palladium / carbon in a suitable solvent such as methanol, ethanol, ethanol / Water, glacial acetic acid, ethyl acetate, dioxane or dimethylformamide are preferably split off at temperatures between 0 and 50 ° C., for example at room temperature, and a hydrogen pressure of 1 to 5 bar.
  • radicals for example a nitro group in an amino group, a benzyloxy group in a hydroxyl group and an N-benzylamino, N-benzylimino, N-benzyloxycarbonylamino or N-benzyloxycarbonylimino group, can simultaneously be converted into a corresponding amino or imino group the.
  • R * - ,, R c , X3, B, D and E are as defined in the introduction and X2 represents a nitrogen atom, with a compound of the general formula
  • R a ' with the exception of the hydrogen atom, has the meanings mentioned for R a or a protective radical for an imino group,
  • Zi is a hydroxy group or a nucleofugic leaving group such as a halogen atom, e.g. a chlorine, bromine or iodine atom, a sulfonic acid ester group, e.g. are a methanesulfonyloxy or p-toluenesulfonyloxy group, an imidazolyl, triazolyl or 4-nitrophenyloxy group and, if appropriate, subsequent cleavage of a protective radical used.
  • a halogen atom e.g. a chlorine, bromine or iodine atom
  • a sulfonic acid ester group e.g. are a methanesulfonyloxy or p-toluenesulfonyloxy group, an imidazolyl, triazolyl or 4-nitrophenyloxy group and, if appropriate, subsequent cleavage of a protective radical used.
  • the reaction is preferably carried out in a solvent such as methanol, ethanol, methylene chloride, tetrahydrofuran, toluene, dioxane, dimethyl sulfoxide or dimethylformamide, optionally in the presence of an inorganic or a tertiary organic base or, if appropriate, in the presence of a dehydrating or acid-activating agent at temperatures performed between -30 and 200 ° C.
  • a solvent such as methanol, ethanol, methylene chloride, tetrahydrofuran, toluene, dioxane, dimethyl sulfoxide or dimethylformamide
  • reaction of a carboxylic acid of the general formula IV is optionally in a solvent or solvent mixture such as methylene chloride, dimethylformamide, benzene, toluene, chlorobenzene, tetrahydrofuran, benzene / tetrahydrofuran or dioxane or in a corresponding amine of the general formula III if appropriate in the presence of a dehydrating agent, for example in the presence of isobutyl chloroformate, tetraethyl orthocarbonate, trimethyl orthoacetate, 2,2-dimethoxypropane, tetramethoxysilane, thionyl chloride, trimethylchlorosilane, phosphorus trichloride, phosphorus pentoxide, N, N'-dimethylcarbodi, N, N'-dimethylcarbodi N'-dicyclohexylcarbodiimide / N-hydroxysuccinimide, N, N '-dic
  • the reaction of a compound of the general formula IV in which Zi represents a nucleofugic leaving group is preferably carried out in a solvent such as methylene chloride, acetonitrile, tetrahydrofuran, dioxane, toluene, dimethylformamide or dimethyl sulfoxide, optionally in the presence of a base such as sodium hydride, Potassium carbonate, potassium tert-butoxide or N-ethyl-di-isopropylamine at temperatures between -20 and 100 ° C, preferably at temperatures between 0 and 60 ° C.
  • a solvent such as methylene chloride, acetonitrile, tetrahydrofuran, dioxane, toluene, dimethylformamide or dimethyl sulfoxide
  • a base such as sodium hydride, Potassium carbonate, potassium tert-butoxide or N-ethyl-di-isopropylamine at temperatures between -20
  • the subsequent cleavage of a protective radical used is advantageously carried out hydrolytically either in the presence of an acid such as hydrochloric acid, sulfuric acid, phosphoric acid, acetic acid, trichloroacetic acid, trifluoroacetic acid or mixtures thereof or in the presence of a base such as lithium hydroxide, sodium hydroxide or potassium hydroxide in one suitable solvents such as water, water / methanol, water / ethanol, water / isopropanol, methanol, ethanol, water / tetrahydrofuran or water / dioxane at temperatures between -10 and 120 ° C, for example at temperatures between room temperature and the boiling temperature of the reaction mixture, or hydrogenolytically in the presence of a hydrogenation catalyst such as palladium / carbon in a suitable Solvents such as methanol, ethanol, ethanol / water, glacial acetic acid, ethyl acetate, dioxane or dimethylformamide preferably at temperatures between
  • R 1, R 3 , C 3 , B, D and E are as defined in the introduction and X 2 'represents a nitrogen atom, with a compound of the general formula
  • R 2 is as defined at the outset
  • R a ' with the exception of the hydrogen atom, has the meanings mentioned for R a or a protective radical for an imino group and
  • Z 2 is a nucleofugic leaving group such as a halogen atom, for example a chlorine, bromine or iodine atom, an imidazolyl, triazolyl or 4-nitrophenyloxy group or
  • Z2 together with R2 represent a further carbon-nitrogen bond and, if appropriate, subsequent cleavage of a protective radical used.
  • the reaction is preferably carried out in a suitable solvent, such as methylene chloride, tetrahydrofuran, toluene, dioxane, dimethyl sulfoxide or dimethylformamide, optionally in the presence of an inorganic or a tertiary organic base or, if appropriate, in the presence of a dehydrating agent at from -30 to 200 ° C. carried out.
  • reaction of a compound of the general formula V, in which Z 2 represents a nucleofugic leaving group, or with an isocyanate of the general formula V is preferably carried out in a solvent such as methylene chloride, acetonitrile, tetrahydrofuran, dioxane, toluene, dimethylformamide or dimethyl sulfoxide, if appropriate in the presence a base such as sodium hydride, potassium carbonate, potassium tert-butoxide or N-ethyl-diisopropylamine at temperatures between -20 and 100 ° C, preferably at temperatures between 0 and 60 ° C.
  • a solvent such as methylene chloride, acetonitrile, tetrahydrofuran, dioxane, toluene, dimethylformamide or dimethyl sulfoxide, if appropriate in the presence a base such as sodium hydride, potassium carbonate, potassium tert-butoxide or N-ethyl-
  • the subsequent cleavage of a protective radical used is advantageously carried out hydrolytically either in the presence of an acid such as hydrochloric acid, sulfuric acid, phosphoric acid, acetic acid, trichloroacetic acid, trifluoroacetic acid or mixtures thereof or in the presence of a base such as lithium hydroxide, sodium hydroxide or potassium hydroxide in one suitable solvents such as water, water / methanol, water / ethanol, water / isopropanol, methanol, ethanol, water / tetrahydrofuran or water / dioxane at temperatures between -10 and 120 ° C., for example at temperatures between room temperature and the boiling point of the reaction mixture, or hydrogenolytically in the presence of a hydrogenation catalyst such as palladium / carbon in a suitable solvent such as methanol, ethanol, ethanol / water, glacial acetic acid, ethyl acetate, dioxane or dimethylformamide, preferably at temperatures between
  • R ⁇ , R c , A, B and Xi to X3 are as defined in the introduction and R a ', with the exception of the hydrogen atom, has the meanings mentioned for R a at the outset or means a protective radical for an imino group, with a compound of the general formula
  • R l , R 4 and E are as defined at the beginning and
  • Z3 is a leaving group such as a halogen atom, e.g. B. a chlorine or bromine atom, or if B is one of the phenylene groups mentioned at the outset, means a hydroxyl group and, if appropriate, subsequent cleavage of a protective radical used.
  • a halogen atom e.g. B. a chlorine or bromine atom
  • B is one of the phenylene groups mentioned at the outset
  • the reaction is advantageously carried out in a solvent such as methylene chloride, tetrahydrofuran, dioxane, dimethyl sulfoxide, dimethylformamide or acetone, optionally in the presence of a reaction accelerator such as sodium or potassium iodide and preferably in the presence of a base such as sodium carbonate or potassium carbonate or in the presence of a tertiary organic base such as N-ethyl-diisopropylamine or N-methyl-morpholine, which can also serve as a solvent, or optionally in the presence of silver carbonate or silver oxide or in the presence of an azodicarboxylic acid diester and a phosphine at temperatures between -30 and the boiling point of solvent used, but preferably at temperatures between -10 and 80 ° C.
  • a solvent such as methylene chloride, tetrahydrofuran, dioxane, dimethyl sulfoxide, dimethylformamide or acetone
  • a reaction accelerator such
  • the reaction is preferably carried out in an aprotic solvent such as diethyl ether, tetrahydrofuran, dioxane, diglyme, benzene or toluene in the presence of a diester of azodicarboxylic acid such as diethyl azodicarboxylic acid and a phosphine such as triphenylphosphine at temperatures between -20 ° C and the boiling point of the solvent used.
  • an aprotic solvent such as diethyl ether, tetrahydrofuran, dioxane, diglyme, benzene or toluene
  • a diester of azodicarboxylic acid such as diethyl azodicarboxylic acid
  • a phosphine such as triphenylphosphine
  • the subsequent cleavage of a protective radical used is advantageously carried out hydrolytically either in the presence of an acid such as hydrochloric acid, sulfuric acid, phosphoric acid, acetic acid, trichloroacetic acid, trifluoroacetic acid or mixtures thereof or in the presence of a base such as lithium hydroxide, sodium hydroxide or potassium hydroxide in one suitable solvents such as water, water / methanol, water / ethanol, water / isopropanol, methanol, ethanol, water / tetrahydrofuran or water / dioxane at temperatures between -10 and 120 ° C., for example at temperatures between room temperature and the boiling point of the reaction mixture, or hydrogenolytically in the presence of a hydrogenation catalyst such as palladium / carbon in a suitable solvent such as methanol, ethanol, ethanol / water, glacial acetic acid, ethyl acetate, dioxane or dimethylformamide, preferably at temperatures between
  • R a to R c , Xi to X3 and A are defined as above and
  • B represents a 3-piperidinylene, 4-piperidinylene or 1,4-piperazinylene group, with a compound of the general
  • the reaction is preferably carried out in a solvent such as methanol, ethanol, methylene chloride, tetrahydrofuran, toluene, dioxane, dimethyl sulfoxide or dimethylformamide, optionally in the presence of a tertiary organic base such as N-ethyldiisopropylamine or N-methylmorpholine at temperatures between -30 and 150 ° C, but preferably at temperatures between 0 and 100 ° C.
  • a solvent such as methanol, ethanol, methylene chloride, tetrahydrofuran, toluene, dioxane, dimethyl sulfoxide or dimethylformamide
  • a tertiary organic base such as N-ethyldiisopropylamine or N-methylmorpholine
  • R a to R c , Xi, X 2 , A, D and E are as defined at the beginning,
  • U is a carbonyl group
  • B is a 3-piperidinylene, 4-piperidinylene or 1,4-piperazinylene group, wherein additionally a 1,4-piperazinylene group by RJ, and R c may be substituted, in which R D and R c as mentioned above, are defined, or
  • the reductive amination is preferably carried out in the presence of a complex metal hydride such as sodium borohydride, lithium borohydride, sodium cyanoborohydride, zinc borohydride, sodium triacetoxyborohydride or borane / pyridine, preferably at a pH of 1-7, optionally in the presence of a dehydrating agent such as molecular sieve or titanium IV isopropylate and at Room temperature or with hydrogen in the presence of a hydrogenation catalyst, for example in the presence of palladium / carbon, at a hydrogen pressure of 1 to 5 bar, preferably at temperatures between 20 ° C. and the boiling point of the solvent used.
  • a complex metal hydride such as sodium borohydride, lithium borohydride, sodium cyanoborohydride, zinc borohydride, sodium triacetoxyborohydride or borane / pyridine
  • a dehydrating agent such as molecular sieve or titanium IV isopropylate
  • a hydrogenation catalyst for example in the
  • R a to R c , Xi to X3, A, B and D are as defined in the introduction, or their reactive derivatives with an alcohol of the general formula
  • R Q an alkyl group with 1 to 6 carbon atoms, a phenylalkyl group in which the alkyl part can contain 1 to 3 carbon atoms, a cycloalkyl group with 3 to 9 carbon atoms, in which the cycloalkyl part with 5 to 8 carbon atoms can additionally be substituted by one or two alkyl groups each having 1 to 3 carbon atoms, a cycloalkyl group having 5 to 8 carbon atoms in which in the cycloalkyl part a methylene group in the 3- or 4-position by an oxygen atom or by an optionally is replaced by an alkyl, phenylalkyl or phenylalkoxycarbonyl group in which the alkyl and alkoxy part can each contain 1 to 3 carbon atoms, or by an alkanoyl group having 2 to 6 carbon atoms substituted imino group and the cycloalkyl part additionally by a or two alkyl groups each having 1 to 3 carbon atoms can
  • R e for R Q has the meanings mentioned above and additionally an R 7 -CO-O- (R 5 CRg) -O group in which
  • R5 to R7 are as defined in the introduction, and
  • Z 4 is a leaving group such as a halogen atom, e.g. B. represent a chlorine or bromine atom.
  • reaction with an alcohol of the general formula XII is advantageously carried out in a solvent or solvent mixture such as methylene chloride, benzene, toluene, chlorobenzene, tetrahydrofuran, benzene / tetrahydrofuran or dioxane, but preferably in an alcohol of the general formula XII, if appropriate in the presence of an acid such as hydrochloric acid or in the presence of a dehydrating agent, e.g.
  • the reaction is expediently carried out in a solvent such as methylene chloride, tetrahydrofuran, dioxane, dimethyl sulfoxide, dimethylformamide or acetone, optionally in the presence of a reaction accelerator such as sodium or potassium iodide and preferably in the presence of a base such as sodium carbonate or potassium carbonate or in the presence of a tertiary organic base such as N-ethyl-diisopropylamine or N-methyl-morpholine, which can also serve as a solvent, or optionally in the presence of silver carbonate or silver oxide at temperatures between -30 and 100 ° C, but preferably at temperatures between -10 and 80 ° C.
  • a solvent such as methylene chloride, tetrahydrofuran, dioxane, dimethyl sulfoxide, dimethylformamide or acetone
  • a reaction accelerator such as sodium or potassium iodide
  • a base such as sodium carbonate or potassium carbonate
  • R a to R c , Xi to X 3 , B and D are as defined above and A "a Represents group, in which Ri is as initially defined.
  • the reduction is preferably carried out in the presence of a complex metal hydride such as sodium borohydride, lithium borohydride, sodium cyanoborohydride, zinc borohydride, sodium triacetoxyborohydride or borane / pyridine, preferably at a pH of 1-7, optionally in the presence of a dehydrating agent such as molecular sieve or titanium IV isopropylate and at room temperature or with hydrogen in the presence of a hydrogenation catalyst, for example in the presence of palladium / carbon, at a hydrogen pressure of 1 to 5 bar, preferably at temperatures between 20 ° C. and the boiling point of the solvent used.
  • a compound of the general formula I is obtained which contains an imino group, this can be converted into the desired alkylated or acylated compound of the general formula I by means of subsequent alkylation or acylation.
  • the subsequent alkylation is optionally carried out in a solvent or solvent mixture such as methylene chloride, dimethylformamide, benzene, toluene, chlorobenzene, tetrahydrofuran, benzene / tetrahydrofuran or dioxane with an alkylating agent such as a corresponding halide or sulfonic acid ester, e.g.
  • methyl iodide ethyl bromide, dimethyl sulfate or benzyl chloride
  • a tertiary organic base preferably at temperatures between 0 and 100 ° C.
  • a complex metal hydride such as sodium borohydride, lithium borohydride or sodium cyanoborohydride
  • a hydrogenation catalyst for example carried out with hydrogen in the presence of palladium / coal, at a hydrogen pressure of 1 to 5 bar.
  • the methylation can also be carried out in the presence of formic acid as a reducing agent at elevated temperatures, e.g. at temperatures between 60 and 120 ° C, are carried
  • the subsequent acylation is optionally carried out with a corresponding reactive carboxylic acid derivative such as the acid halide in a solvent or solvent mixture such as methylene chloride, dimethylformamide, benzene, toluene, chlorobenzene, tetrahydrofuran or dioxane, optionally in the presence of a tertiary organic base or in the presence an inorganic base or with a corresponding carboxylic acid in the presence of a dehydrating agent, for example in the presence of isobutyl chloroformate, thionyl chloride, tri- methylchlorosilane, phosphorus trichloride, 2- (1H-benzotriazol-1-yl) -1,1,3,3-tetramethyluronium tetrafluoroborate, N, N'-dicyclohexylcarbodiimide, N, N'-dicyclohexylcarbodiimide / N-hydroxysuccinimide or l
  • any reactive groups present such as hydroxyl, carboxy, amino, alkylamino or imino groups, can be protected during the reaction by customary protective groups, which are split off again after the reaction.
  • the trimethylsilyl, acetyl, benzoyl, tert-butyl, trityl, benzyl or tetrahydropyrany1 group comes as a protective radical for a hydroxyl group
  • a protective radical for an amino, alkylamino or imino group the formyl, acetyl, trifluoroacetyl, methoxycarbonyl, ethoxycarbonyl, tert.butoxycarbonyl, benzyloxycarbonyl, benzyl, methoxybenzyl or 2,4-dimethoxybenzyl group for the imino group in addition the methyl group and the phthalyl group for the amino group.
  • the subsequent subsequent splitting off of a protective residue used takes place, for example, hydrolytically in an aqueous solvent, for example in water, isopropanol / water, acetic acid / water, tetrahydrofuran / water or dioxane / water, in the presence of an acid such as trifluoroacetic acid, hydrochloric acid or Sulfuric acid or in the presence of an alkali base such as sodium hydroxide or potassium hydroxide or by means of ether cleavage, for example in Presence of iodotrimethylsilane, at temperatures between 0 and 120 ° C, preferably at temperatures between 10 and 100 ° C.
  • an aqueous solvent for example in water, isopropanol / water, acetic acid / water, tetrahydrofuran / water or dioxane / water, in the presence of an acid such as trifluoroacetic acid, hydrochloric acid or Sulfuric acid or in the presence
  • a benzyl, methoxybenzyl or benzyloxycarbonyl radical is split off, for example by hydrogenolysis, e.g. with hydrogen in the presence of a catalyst such as palladium / carbon in a solvent such as methanol, ethanol, isopropanol, ethyl acetate or glacial acetic acid, optionally with the addition of an acid such as hydrochloric acid at temperatures between 0 and 100 ° C, but preferably at temperatures between ⁇ 20 and 60 ° C, and at a hydrogen pressure of 1 to 7 bar, but preferably from 3 to 5 bar.
  • a catalyst such as palladium / carbon
  • a solvent such as methanol, ethanol, isopropanol, ethyl acetate or glacial acetic acid
  • an acid such as hydrochloric acid
  • a tert-butyl or tert-butyloxycarbonyl radical is preferably cleaved off by treatment with an acid such as trifluoroacetic acid or hydrochloric acid or by treatment with iodotrimethylsilane, optionally using a solvent such as methylene chloride, dioxane, methanol or ether.
  • a trifluoroacetyl radical is preferably cleaved by treatment with an acid such as hydrochloric acid, if appropriate in the presence of a solvent such as acetic acid or methanol at temperatures between 50 and 120 ° C. or by treatment with sodium hydroxide solution if appropriate in the presence of a solvent such as tetrahydrofuran or methanol at temperatures between between 0 and 50 ° C.
  • a methyl group is preferably cleaved from a methylimino group in the presence of 1-chloroalkyl chloroformate such as 1-chloroethyl chloroformate, preferably in the presence of a base such as 1,8-bis (dimethylamino) naphthalene in the presence of a solvent such as methylene chloride , 1,2-dichloroethane, toluene or dioxane at temperatures between 0 and 150 ° C, preferably at temperatures between 20 ° C and the boiling point of the reaction mixture, and subsequent treatment with an alcohol such as methanol at temperature temperatures between 20 ° C and the boiling point of the alcohol used.
  • 1-chloroalkyl chloroformate such as 1-chloroethyl chloroformate
  • a base such as 1,8-bis (dimethylamino) naphthalene
  • a solvent such as methylene chloride , 1,2-dichloroethane, toluen
  • a phthalyl radical is preferably cleaved in the presence of hydrazine or a primary amine such as methylamine, ethylamine or n-butylamine in a solvent such as methanol, ethanol, isopropanol, toluene / water or dioxane at temperatures between 20 and 50 ° C.
  • the compounds of general formula I obtained can be separated into their enantiomers and / or diastereomers.
  • cis / trans mixtures can be separated into their eis and trans isomers, and chiral compounds into their enantiomers.
  • the cis / trans mixtures obtained can be chromatographed into their eis and trans isomers, the compounds of general formula I obtained which occur in racemates, according to methods known per se (see Allinger NL and Eliel EL in "Topics in Stereochemistry", Vol. 6, Wiley Interscience, 1971) in their optical antipodes and compounds of general formula I with at least 2 stereogenic centers on the basis of their physico-chemical differences according to methods known per se, for example by chromatography and / or fractional crystallization, into their diastereomers, which, if they occur in racemic form, can then be separated into the enantiomers as mentioned above.
  • the enantiomers are separated preferably by column separation on chiral phases or by recrystallization from an optically active solvent or by reaction with an optically active substance which forms salts or derivatives such as esters or amides, in particular acids and their acids, with the racemic compound activated derivatives or alcohols, and separating the diastereomeric salt mixtures or derivatives, for example on the basis of different solubilities, it being possible for the free antipodes to be released from the pure diastereomeric salts or derivatives by the action of suitable agents.
  • optically active acids are, for example, the D and L forms of tartaric acid or dibenzoyl tartaric acid, di-o-tolyltartaric acid, malic acid, mandelic acid, camphorsulfonic acid, glutamic acid, aspartic acid or quinic acid.
  • suitable optically active alcohols are (+) - or (-) menthol and optically active acyl radicals in amides are, for example, (+) or (-) menthyloxycarbonyl.
  • the compounds of the formula I obtained can be converted into their salts, in particular for pharmaceutical use into their physiologically tolerable salts with inorganic or organic acids.
  • Suitable acids are, for example, hydrochloric acid, hydrobromic acid, sulfuric acid, phosphoric acid, fumaric acid, succinic acid, lactic acid, citric acid, tartaric acid or maleic acid.
  • the new compounds of formula I thus obtained can, if desired, subsequently be converted into their salts with inorganic or organic bases, in particular for their pharmaceutical use into their physiologically tolerable salts.
  • suitable bases are sodium hydroxide, potassium hydroxide, arginine, cyclohexylamine, ethanolamine, diethanolamine and triethanolamine.
  • the new carboxylic acid derivatives of the general formula I and their salts in particular their physiologically tolerable salts with inorganic or organic acids or bases, have valuable properties, in particular, valuable pharmacological properties, in addition to an anti-inflammatory and bone-depleting effect, in particular antithrombotic, antiaggregatory and anti-tumor or metastatic effects.
  • donor blood is drawn from an antecubital vein and anticoagulated with trisodium citrate (final concentration 13 mM).
  • the blood is centrifuged at 170 xg for 10 minutes and the supernatant platelet-rich plasma (PRP) is removed. The remaining blood is sharply centrifuged off again to obtain plasma.
  • the PRP is diluted 1:10 with autologous plasma. 750 ⁇ l are incubated with 50 ml physiological saline, 100 ⁇ l test substance solution, 50 ⁇ l 14 C-sucrose (3,700 Bq) and 50 ⁇ l 3 H-BIBU 52 (final concentration: 5 nM) at room temperature for 20 minutes.
  • 100 ⁇ l BIBU 52 (final concentration: 30 ⁇ M) is used to measure the non-specific binding.
  • the samples are centrifuged at 10,000 xg for 20 seconds and the supernatant is removed. 100 ⁇ l of this are measured to determine the free ligand.
  • the pellet is dissolved in 500 ul 0.2N NaOH, 450 ul are mixed with 2 ml scintillator and 25 ul 5N HCl and measured. The residual plasma remaining in the pellet is determined from the 14 C content, the bound ligand from the 3 H measurement. After deduction of the non-specific binding, the pellet activity is plotted against the concentration of the test substance and the concentration for a 50% inhibition of binding is determined.
  • Platelet aggregation is measured according to the method of Born and Cross (J. Physiol. 170, 397 (1964)) in platelet-rich plasma from healthy test subjects. To inhibit coagulation, the blood is mixed with 3.14% sodium citrate in a volume ratio of 1:10.
  • the course of the decrease in the optical density of the platelet suspension is measured and recorded photometrically after the addition of the aggregation-triggering substance.
  • the rate of aggregation is inferred from the angle of inclination of the density curve.
  • the point on the curve at which the greatest light transmittance is present is used to calculate the "optical density".
  • the amount of collagen is chosen to be as small as possible, but in such a way that an irreversible reaction curve results.
  • the commercial collagen from Hormonchemie, Kunststoff, is used.
  • the plasma is incubated with the substance at 37 ° C. for 10 minutes.
  • the compounds of Examples 2, 9, 9 (1), 9 (2) and 2 (4) on rhesus monkeys after oral administration of 1 mg / kg have high plasma levels over a period of more than 8 hours.
  • the new compounds are well tolerated since, for example, after intravenous administration of 100 mg / kg of the compound according to the invention of the above examples, no toxic side effects could be observed on the mouse.
  • the new carboxylic acid derivatives of general formula I and their physiologically tolerable salts are suitable for combating or preventing diseases in which smaller or larger cell aggregates occur or Cell matrix interactions play a role, for example in combating or preventing venous and arterial thrombosis, cerebrovascular diseases, pulmonary embolism, heart attack, arteriosclerosis, osteoporosis and the metastasis of tumors and the therapy of genetically determined or also acquired disturbances in the interactions of cells with one another or with solid structures.
  • These are also suitable for accompanying therapy for thrombosis with fibrinolytics or vascular Interventions such as transluminal angioplasty or also in the treatment of shock, psoriasis, diabetes and inflammation.
  • the dose is between 0.1 mg and 30 mg / kg body weight, preferably 1 mg to 15 mg / kg body weight, with up to 4 doses per day.
  • the compounds of the formula I prepared according to the invention optionally in combination with other active substances such as thromboxane receptor antagonists and thromboxane synthesis inhibitors or their combinations, serotonin antagonists, ⁇ -receptor antagonists, alkyl nitrates such as glycerol trinitrate, phosphodiesterase inhibitors, prostacyclin and their Analogs, fibrinolytics such as tPA, prourokinase, urokinase, streptokinase, or anticoagulants such as heparin, dermatan sulfate, activated protein C, vitamin K antagonists, hirudin, inhibitors of thrombin or other activated coagulation factors, together with one or more inert ones usual carriers and
  • the combined ethyl acetate extracts are dried and evaporated to dryness in vacuo.
  • the catalyst is filtered off, the filtrate under vacuum
  • a suspension of 6 g (0.0205 mol) of 4-tert-butyloxycarbonyl-1- (4-hydroxyphenyl) -2-methyl-piperazine, 2.4 ml (0.0246 mol) of methyl bromoacetate and 3.4 g (0.0246 mol) of potassium carbonate in 50 ml of dimethylformamide is heated to 100 ° C. for 6 hours and, after cooling under reduced pressure, evaporated to dryness. The residue is partitioned between ethyl acetate and water and the aqueous phase is extracted again with ethyl acetate. The combined ethyl acetate extracts are evaporated to dryness in vacuo.
  • the organic phase is separated off and the aqueous phase is extracted with 20 ml of methylene chloride.
  • the combined organic phases are washed with water, dried over sodium sulfate and evaporated to dryness under reduced pressure.
  • the remaining residue (1.3 g) is left in 2.5 ml of trifluoroacetic acid overnight at room temperature and then 0.5 g of p-toluenesulfonic acid is added and the mixture is stirred at 70-75 ° C. for 4 hours. After cooling, the toluene solution is washed with aqueous sodium bicarbonate solution and evaporated to dryness under reduced pressure.
  • Rf value 0.35 (silica gel; methylene chloride / methanol / conc.
  • Rf value 0.065 (silica gel; methylene chloride / methanol / conc.
  • trans-4- [(l-tert-butyloxycarbonyl-piperidin-4-yl) methyloxy! -1- (4-methoxycarbonylmethylox ⁇ -phenyl) cyclohexane Made from trans-4- [(l-tert-butyloxycarbonylpiperidin-4-yl) methyloxy] -1- (4-hydroxyphenyl) cyclohexane and bromoacetic acid, methyl ester.
  • Active ingredient and mannitol are dissolved in water. After filling, freeze-drying. The ready-to-use solution is dissolved with water for injections.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Diabetes (AREA)
  • Hematology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Plural Heterocyclic Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Hydrogenated Pyridines (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)

Abstract

L'invention a pour objet des dérivés d'acides carboxyliques de formule générale (I), dans laquelle Ra à Rc, A, B, D, E et X1 à X3 sont tels que définis dans la revendication 1, leurs tautomères, leur stéréoisomères, y compris leurs mélanges et leurs sels, en particulier leurs sels physiologiquement tolérés avec des acides organiques ou des bases, lesquels présentent des propriétés pharmacologiques remarquables, avantageusement des effets inhibant l'agrégation. L'invention a également pour objet des médicaments renfermant ces composés, leur utilisation et leurs procédés de fabrication.
EP97918113A 1996-04-10 1997-04-04 Derives d'acides carboxyliques a effet inhibant l'agregation Withdrawn EP0892783A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE19614204A DE19614204A1 (de) 1996-04-10 1996-04-10 Carbonsäurederivate, diese Verbindungen enthaltende Arzneimittel, deren Verwendung und Verfahren zu ihrer Herstellung
DE19614204 1996-04-10
PCT/EP1997/001698 WO1997037975A1 (fr) 1996-04-10 1997-04-04 Derives d'acides carboxyliques a effet inhibant l'agregation

Publications (1)

Publication Number Publication Date
EP0892783A1 true EP0892783A1 (fr) 1999-01-27

Family

ID=7790922

Family Applications (1)

Application Number Title Priority Date Filing Date
EP97918113A Withdrawn EP0892783A1 (fr) 1996-04-10 1997-04-04 Derives d'acides carboxyliques a effet inhibant l'agregation

Country Status (9)

Country Link
US (1) US5994356A (fr)
EP (1) EP0892783A1 (fr)
JP (1) JP2000508307A (fr)
AR (1) AR006569A1 (fr)
AU (1) AU2636897A (fr)
CO (1) CO4560551A1 (fr)
DE (1) DE19614204A1 (fr)
WO (1) WO1997037975A1 (fr)
ZA (1) ZA973002B (fr)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6964974B2 (en) 2000-09-08 2005-11-15 Hoffmann-La Roche Inc. 2,3-oxidosqualene-lanosterol cyclase inhibitors
US7091230B2 (en) * 2001-02-09 2006-08-15 Merck & Co., Inc. 2-aryloxy-2-arylalkanoic acids for diabetes and lipid disorders
PE20030705A1 (es) * 2001-10-17 2003-08-21 Schering Corp Inhibidores de la 17beta-hidroxiesteroide deshidrogenasa tipo 3 para el tratamiento de enfermedades androgeno-dependientes
US7390813B1 (en) 2001-12-21 2008-06-24 Xenon Pharmaceuticals Inc. Pyridylpiperazines and aminonicotinamides and their use as therapeutic agents
US20050119251A1 (en) * 2001-12-21 2005-06-02 Jian-Min Fu Nicotinamide derivatives and their use as therapeutic agents
ATE425966T1 (de) * 2002-11-26 2009-04-15 Pfizer Prod Inc Durch phenyl subtituierten piperidinverbindungen zur verwendung als ppar-aktivatoren
PE20040769A1 (es) * 2002-12-18 2004-11-06 Schering Corp Derivados de piperidina utiles como antagonisas ccr5
TW200508224A (en) 2003-02-12 2005-03-01 Bristol Myers Squibb Co Cyclic derivatives as modulators of chemokine receptor activity
EP3042895A1 (fr) * 2003-07-30 2016-07-13 Xenon Pharmaceuticals Inc. Dérivés de la pipérazine et leur utilisation en tant qu'agents thérapeutiques
AU2004261249B2 (en) * 2003-07-30 2008-09-04 Xenon Pharmaceuticals Inc. Pyridazine derivatives and their use as therapeutic agents
US7754711B2 (en) 2003-07-30 2010-07-13 Xenon Pharmaceuticals Inc. Pyridazine derivatives and their use as therapeutic agents
US7759348B2 (en) * 2003-07-30 2010-07-20 Xenon Pharmaceuticals Inc. Pyridazine derivatives and their use as therapeutic agents
SE0401971D0 (sv) * 2004-08-02 2004-08-02 Astrazeneca Ab Piperidne derivatives
EP1799667B1 (fr) * 2004-09-20 2013-03-20 Xenon Pharmaceuticals Inc. Derives heterocycliques et leur utilisation comme agents therapeutiques
EP2269610A3 (fr) * 2004-09-20 2011-03-09 Xenon Pharmaceuticals Inc. Dérivés hétérocycliques et leur utilisation en tant qu'inhibiteurs de la stearoyl-coa desaturase
JP4958786B2 (ja) 2004-09-20 2012-06-20 ゼノン・ファーマシューティカルズ・インコーポレイテッド 複素環誘導体および治療薬としてのそれらの使用
MX2007003332A (es) 2004-09-20 2007-06-05 Xenon Pharmaceuticals Inc Derivados heterociclicos y su uso como inhibidores de estearoil-coa-desaturasa.
EP2289510A1 (fr) 2004-09-20 2011-03-02 Xenon Pharmaceuticals Inc. Dérivés hétérocycliques pour le traitement des maladies induites par des enzymes stearoyl-coa desaturase
MX2007003327A (es) * 2004-09-20 2007-06-05 Xenon Pharmaceuticals Inc Derivados heterociclicos, y su uso como mediadores de estearoil-coa desaturasa.
MX2007003319A (es) * 2004-09-20 2007-06-05 Xenon Pharmaceuticals Inc Derivados heterociclicos y su uso como agentes terapeuticos.
US20080167321A1 (en) * 2004-09-20 2008-07-10 Xenon Pharmaceuticals Inc. Pyridine Derivatives For Inhibiting Human Stearoyl-Coa-Desaturase
BRPI0611187A2 (pt) * 2005-06-03 2010-08-24 Xenon Pharmaceuticals Inc derivados aminotiazàis como inibidores da estearoil-coa desaturase humana
US20090203676A1 (en) * 2005-06-30 2009-08-13 Oscar Barba G-protein Coupled Receptor Agonists
GB0514811D0 (en) * 2005-07-19 2005-08-24 Glaxo Group Ltd Compounds
US20070197590A1 (en) * 2006-01-31 2007-08-23 Demong Duane E Substituted dipiperidine ccr2 antagonists
EP3191453B1 (fr) 2014-09-09 2019-10-23 Bristol-Myers Squibb Company Modulateurs des récepteurs gpr120 à base d'acide phényl-(aza)cycloalkylcarboxylique
AU2023353560A1 (en) * 2022-09-30 2025-04-03 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Dopamine d3/d2 receptor partial agonists for the treatment of neuropsychiatric disorders

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4127404A1 (de) * 1991-08-19 1993-02-25 Thomae Gmbh Dr K Cyclische iminoderivate, diese verbindungen enthaltende arzneimittel und verfahren zu ihrer herstellung
US5227490A (en) * 1992-02-21 1993-07-13 Merck & Co., Inc. Fibrinogen receptor antagonists
DE4241632A1 (de) * 1992-12-10 1994-06-16 Thomae Gmbh Dr K Carbonsäurederivate, diese Verbindungen enthaltende Arzneimittel und Verfahren zu ihrer Herstellung
US5652242A (en) * 1993-03-29 1997-07-29 Zeneca Limited Heterocyclic derivatives
DE4326344A1 (de) * 1993-08-05 1995-02-09 Thomae Gmbh Dr K Carbonamide, diese Verbindungen enthaltende Arzneimittel und Verfahren zu ihrer Herstellung

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO9737975A1 *

Also Published As

Publication number Publication date
CO4560551A1 (es) 1998-02-10
WO1997037975A1 (fr) 1997-10-16
AR006569A1 (es) 1999-09-08
DE19614204A1 (de) 1997-10-16
US5994356A (en) 1999-11-30
ZA973002B (en) 1998-10-09
AU2636897A (en) 1997-10-29
JP2000508307A (ja) 2000-07-04

Similar Documents

Publication Publication Date Title
WO1997037975A1 (fr) Derives d'acides carboxyliques a effet inhibant l'agregation
DE602004006891T2 (de) Derivate von n-äphenyl(pyrrolidin-2-yl)methylübenzamid und n-ä(azepan-2-yl)phenylmethylübenzamid, verfahren zu deren herstellung und deren anwendung in therapeutika
DE602004005886T2 (de) Derivate von n-äphenyl(alkylpiperidin-2-yl)methylübenzamid, verfahren zu deren herstellung und deren anwendung in therapeutika
DE69103206T2 (de) Neue Derivate des Aminopiperidins, Aminopyrrolidins und des Aminoperhydroazepins, Verfahren zur Herstellung und diese enthaltende Arzneimittel.
DE602004006431T2 (de) Derivate von n-äheteroaryl(piperidin-2-yl)methylübenzamid, verfahren zu deren herstellung und deren anwendung in therapeutika
EP0592949A2 (fr) Dérivés de N-(aminocarbonyl)-pipéridine/pipérazine comme agents inhibitant l'aggrégation
DE60225067T2 (de) Pyrrolidin und piperidinderivate als nk1 antagonisten
EP0718287A2 (fr) Dérivés de pipérazine, médicaments les contenant, leur application, et procédés pour leur préparation
DE69221919T2 (de) Piperidin-Derivate
EP0799202A1 (fr) Derives de piperazine, medicaments contenant ces composes, leur utilisation et leur procede de preparation
DE4241632A1 (de) Carbonsäurederivate, diese Verbindungen enthaltende Arzneimittel und Verfahren zu ihrer Herstellung
EP0574808A1 (fr) Dérivés des amidines-biphenyles, un procédé pour leurs préparation et des médicaments les contenant
DE4213919A1 (de) Cyclische iminoderivate, verfahren zu ihrer herstellung und diese verbindungen enthaltende arzneimittel
DE19643331A1 (de) 1-(4-Piperidinyl)-piperidinylene, diese Verbindungen enthaltende Arzneimittel, deren Verwendung und Verfahren zu ihrer Herstellung
DE4326344A1 (de) Carbonamide, diese Verbindungen enthaltende Arzneimittel und Verfahren zu ihrer Herstellung
DE60213629T2 (de) Piperidinverbindungen als muscarinantagonisten
WO1996004271A1 (fr) Derives condenses d'azepine, medicaments les contenant et leurs procedes de preparation
DE4446301A1 (de) Piperazinderivate, diese Verbindungen enthaltende Arzneimittel, deren Verwendung und Verfahren zu ihrer Herstellung
EP0824515B1 (fr) Phenylamidines substituees, medicaments contenant ces composes et leurs procedes de preparation
KR20130101503A (ko) 5ht2b 수용체 길항제
DE3000625A1 (de) Cis- und trans-isomere von 3-aryloxy- 4-hydroxypyrrolidinen und deren derivaten, verfahren zu ihrer herstellung und ihre verwendung
WO1995024405A1 (fr) Imidazolidin-2-ones, medicaments contenant ces composes et leur procede de preparation
DE19548798A1 (de) 5-gliedrige Heterocyclen, diese Verbindungen enthaltende Arzneimittel und deren Verwendung sowie Verfahren zu ihrer Herstellung
WO1996002504A1 (fr) Biphenyloxymethyl-6 carboxy-4 piperidinones-2, medicaments renfermant ces composes et leur procede de fabrication
WO2000005207A1 (fr) Phenylamidines substituees a effet antithrombotique

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 19981110

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

17Q First examination report despatched

Effective date: 20030211

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20030624