EP0972191A1 - Dispositif de chromatographie annulaire continue - Google Patents

Dispositif de chromatographie annulaire continue

Info

Publication number
EP0972191A1
EP0972191A1 EP98909215A EP98909215A EP0972191A1 EP 0972191 A1 EP0972191 A1 EP 0972191A1 EP 98909215 A EP98909215 A EP 98909215A EP 98909215 A EP98909215 A EP 98909215A EP 0972191 A1 EP0972191 A1 EP 0972191A1
Authority
EP
European Patent Office
Prior art keywords
particle bed
bed
particle
chromatography
access opening
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP98909215A
Other languages
German (de)
English (en)
Inventor
Adalbert Prior
Jürgen Wolfgang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Prior Separation Technology GmbH
Original Assignee
Prior Separation Technology GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Prior Separation Technology GmbH filed Critical Prior Separation Technology GmbH
Publication of EP0972191A1 publication Critical patent/EP0972191A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/50—Conditioning of the sorbent material or stationary liquid
    • G01N30/58—Conditioning of the sorbent material or stationary liquid the sorbent moving as a whole
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/50—Conditioning of the sorbent material or stationary liquid
    • G01N30/56—Packing methods or coating methods
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/50—Conditioning of the sorbent material or stationary liquid
    • G01N30/56—Packing methods or coating methods
    • G01N2030/562—Packing methods or coating methods packing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/50—Conditioning of the sorbent material or stationary liquid
    • G01N30/58—Conditioning of the sorbent material or stationary liquid the sorbent moving as a whole
    • G01N2030/582—Conditioning of the sorbent material or stationary liquid the sorbent moving as a whole micellar electrokinetic capillary chromatography [MECC]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/50—Conditioning of the sorbent material or stationary liquid
    • G01N30/58—Conditioning of the sorbent material or stationary liquid the sorbent moving as a whole
    • G01N2030/587—Continuous annular chromatography

Definitions

  • the invention relates to a device for continuous annular chromatography, in which a relative movement between a particle bed in the form of a cylindrical jacket and at least one feed point for liquid feed material is provided.
  • a standing cylinder is filled with particulate material and charged with a mixture of components to be separated from the top. Then, a solvent is used to continuously elute from above - due to their different affinity for the particle material, the components are separated and left in proportion to the flow rate of the solvent (eluent) the column one by one.
  • the invention relates to a further development of all known devices for annular chromatography and among other things. the task of being able to replace the particle bed without disassembling the device
  • the device according to the invention is primarily characterized in that at least one access opening to the particle bed, in particular for bed material removal, is provided at the lower end of the particle bed space
  • the bed material can be emptied downwards, preferably rinsed with liquid, and the device can be reloaded from above.
  • the access opening can also be used to rewind with pressure medium, e.g. with Compressed air the bed is loosened
  • the access opening is preferably a ring opening and is in particular closed and opened by changing the volume of a hollow seal over its interior.
  • the access opening is advantageously formed by collapsing a hollow seal by applying negative pressure
  • FIGS. 1 and 2 are schematic sectional side views, which show a device for annular chromatography with a stationary feed head and rotating particle bed.
  • Corresponding components have the same reference numerals
  • a fixed support platform 2 and a displaceable support arm 3 are arranged on a stand 1.
  • the support platform 2 is penetrated by a rotor table 4 which is driven from below and is supported on the support platform 2 via a tapered roller bearing 5 and on which a base plate 6, one of which is bolted on Central elevation 7 shows, on which a cylindrical hollow body 8 with a conical roof 9 is placed.
  • a cylinder jacket 10 is bolted onto the base plate 6 from above, a vertical annular space 11 between the hollow body 8 and cylinder jacket 10 and between the lower edge of the Hollow body 8 and the top of the bottom plate 6 remains a horizontal gap 12 extending inwards to the central elevation 7, in which an annular hollow seal 13 is arranged.
  • Through holes 14 are provided which connect the horizontal gap 12 to the underside 15 of the base plate 6.
  • the cylinder jacket 10 carries a cover 16 and this a bearing sleeve 1 7 with a tapered roller bearing 18, which is supported centrally on a test stand, held by the support arm 3 feeder unit 1 9, so that all the components placed on the rotor table 4 in operation with respect to the support platform 2 and the Feeder unit 19 are rotated.
  • the vertical annular space 1 1 is filled with particulate bed material, which e.g. is a gel material, an ion exchange material or the like and, as indicated at 20, can be supplied via the feeder unit 19.
  • particulate bed material e.g. is a gel material, an ion exchange material or the like and, as indicated at 20, can be supplied via the feeder unit 19.
  • liquid feed material is fed in there, and centrally at 21 an eluent, which flows off via a distributor studio 22 and the conical roof 9 to the bed material in room 11.
  • the bottom plate 6 is penetrated by take-off nozzles 23 distributed over a circumference, and the support platform 2 carries a collecting trough 24 for receiving collecting vessels (not shown). With 25 a lateral discharge pipe is designated.
  • the hollow seal 13 is collapsed via the vacuum line 26, as a result of which the horizontal gap 12 opens toward the bores 14 and the bed material is spooled into the collecting trough 24 With 27 a ventilation or compressed air line is indicated, with which the hollow seal 1 3 can be expanded during the operation of the plant.
  • the device shown in FIG. 2 does not have a volume-adjustable seal for opening and closing an annular gap, but the entire hollow body 8 is lifted off the base plate 6 via a hydraulic or pneumatic cylinder piston unit 28, 29, which is located on the central part 7 of the base plate is supported by a foot 30.
  • the lower ring edge 31 of the hollow body 8 is made from a corresponding annular groove 32 in the base plate 6 lifted out, in which he sits sealed in the idle state.
  • the through holes 14 are arranged in the bottom of the annular groove 32. Otherwise, the embodiments according to FIGS. 1 and 2 and their operation are identical.

Landscapes

  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)

Abstract

L'invention concerne un dispositif de chromatographie annulaire continue dans lequel un lit de particules sous forme d'une enveloppe de cylindre et au moins un point de chargement d'une matière liquide sont mobiles l'un par rapport à l'autre. L'extrémité inférieure du compartiment (11) du lit de particules possède au moins une ouverture (12, 32) d'accès au lit de particules, notamment pour le transport de la matière constituant le lit.
EP98909215A 1997-04-03 1998-03-25 Dispositif de chromatographie annulaire continue Withdrawn EP0972191A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
AT0056197A AT404904B (de) 1997-04-03 1997-04-03 Vorrichtung zur kontinuierlichen annularen chromatographie
AT56197 1997-04-03
PCT/AT1998/000080 WO1998045699A1 (fr) 1997-04-03 1998-03-25 Dispositif de chromatographie annulaire continue

Publications (1)

Publication Number Publication Date
EP0972191A1 true EP0972191A1 (fr) 2000-01-19

Family

ID=3493872

Family Applications (1)

Application Number Title Priority Date Filing Date
EP98909215A Withdrawn EP0972191A1 (fr) 1997-04-03 1998-03-25 Dispositif de chromatographie annulaire continue

Country Status (9)

Country Link
EP (1) EP0972191A1 (fr)
JP (1) JP2001519906A (fr)
CN (1) CN1307679A (fr)
AT (1) AT404904B (fr)
AU (1) AU725543B2 (fr)
BR (1) BR9809569A (fr)
CA (1) CA2285425A1 (fr)
EA (1) EA199900898A1 (fr)
WO (1) WO1998045699A1 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1657547A1 (fr) * 2000-03-07 2006-05-17 Prior Engineering Ag Chromatographie annulaire
EP1300415B1 (fr) 2001-10-03 2007-01-24 Boehringer Ingelheim International GmbH Méthode pour la réconstitution d'une protéine recombinant active
PE20030701A1 (es) 2001-12-20 2003-08-21 Schering Corp Compuestos para el tratamiento de trastornos inflamatorios

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4675113A (en) * 1984-11-28 1987-06-23 University Patents, Inc. Affinity chromatography using dried calcium alginate-magnetite separation media in a magnetically stabilized fluidized bed
US4683042A (en) * 1986-04-29 1987-07-28 The United States Of America As Represented By The United States Department Of Energy Method and apparatus for continuous annular electrochromatography
US5149436A (en) * 1988-11-28 1992-09-22 Union Oil Company Of California Continuous displacement chromatographic method
US5045209A (en) * 1990-09-24 1991-09-03 Westinghouse Electric Corp. Method for chromatographically recovering scandium and yttrium
US5112493A (en) * 1990-12-10 1992-05-12 Westinghouse Electric Corp. Zirconium-hafnium production in a zero liquid discharge process
DE4117604A1 (de) * 1991-05-29 1992-12-03 Merck Patent Gmbh Verfahren und vorrichtung zur befuellung von chromatographiesaeulen
US5273656A (en) * 1991-09-04 1993-12-28 Large Scale Biology Corporation System for solid phase reactions

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO9845699A1 *

Also Published As

Publication number Publication date
CN1307679A (zh) 2001-08-08
AT404904B (de) 1999-03-25
EA199900898A1 (ru) 2000-06-26
BR9809569A (pt) 2000-10-17
AU6385398A (en) 1998-10-30
AU725543B2 (en) 2000-10-12
JP2001519906A (ja) 2001-10-23
WO1998045699A1 (fr) 1998-10-15
ATA56197A (de) 1998-08-15
CA2285425A1 (fr) 1998-10-15

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