EP0979233A4 - Verfahren zur synthese von oligonukleotiden in lösung - Google Patents

Verfahren zur synthese von oligonukleotiden in lösung

Info

Publication number: EP0979233A4
Authority: EP; European Patent Office
Prior art keywords: oligonucleotides; synthesis; solution
Prior art date: 1997-04-21
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Withdrawn

Application number

EP98918629A

Other languages

English (en)

French (fr)

Other versions

EP0979233A1 (de

Inventor

Wolfgang Pieken

Danny Mcgee

Alecia Settle

Yansheng Zhai

Jianping Huang

Ken Hill

Randall S Smith

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Sigma Aldrich Co LLC

Original Assignee

Proligo LLC

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1997-04-21

Filing date

1998-04-20

Publication date

2001-03-21

1998-04-20 Application filed by Proligo LLC filed Critical Proligo LLC

2000-02-16 Publication of EP0979233A1 publication Critical patent/EP0979233A1/de

2001-03-21 Publication of EP0979233A4 publication Critical patent/EP0979233A4/de

Status Withdrawn legal-status Critical Current

Links

108091034117 Oligonucleotide Proteins 0.000 title 1
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 title 1
230000015572 biosynthetic process Effects 0.000 title 1
238000000034 method Methods 0.000 title 1
238000003786 synthesis reaction Methods 0.000 title 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom

Landscapes

Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
Biochemistry (AREA)
Biotechnology (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Molecular Biology (AREA)
Saccharide Compounds (AREA)

EP98918629A 1997-04-21 1998-04-20 Verfahren zur synthese von oligonukleotiden in lösung Withdrawn EP0979233A4 (de)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
US84382097A	1997-04-21	1997-04-21
US843820		1997-04-21
PCT/US1998/008192 WO1998047910A1 (en)	1997-04-21	1998-04-20	Method for solution phase synthesis of oligonucleotides

Publications (2)

Publication Number	Publication Date
EP0979233A1 EP0979233A1 (de)	2000-02-16
EP0979233A4 true EP0979233A4 (de)	2001-03-21

Family

ID=25291099

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
EP98918629A Withdrawn EP0979233A4 (de)	1997-04-21	1998-04-20	Verfahren zur synthese von oligonukleotiden in lösung

Country Status (6)

Country	Link
EP (1)	EP0979233A4 (de)
JP (1)	JP2001520660A (de)
AU (1)	AU7152098A (de)
CA (1)	CA2286320A1 (de)
IL (1)	IL132377A0 (de)
WO (1)	WO1998047910A1 (de)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US7427678B2 (en)	1998-01-08	2008-09-23	Sigma-Aldrich Co.	Method for immobilizing oligonucleotides employing the cycloaddition bioconjugation method
GB9825687D0 (en) *	1998-11-25	1999-01-20	Link Technologies Ltd	Oligonucleotide conjugation
JP2003535317A (ja) *	2000-05-01	2003-11-25	プロリゴ・エルエルシー	付加環化バイオコンジュゲーション法を利用してオリゴヌクレオチドを固定化する方法
DE10041221A1 (de)	2000-08-22	2002-03-14	Deutsches Krebsforsch	Verfahren zur Herstellung von wasserlöslichen Saccharidkonjugaten und Saccharidmimetika durch Diels-Alder-Reaktion und ihre Verwendung als Therapeutika oder Diagnostika
US7172905B2 (en)	2001-08-07	2007-02-06	The University Of Chicago	Polypeptide immobilization
AU2003210629A1 (en)	2002-01-23	2003-09-02	Proligo, Llc	Methods for the integrated synthesis and purification of oligonucleotides
GB0229423D0 (en) *	2002-12-18	2003-01-22	Avecia Ltd	Process
EP1585755B1 (de)	2002-12-31	2015-08-05	Sigma-Aldrich Co. LLC	Verfahren und zusammenstellungen für die tandem-synthese von zwei oder mehr oligonukleotiden auf einen festen träger
JP4945129B2 (ja) *	2004-01-27	2012-06-06	株式会社キラルジェン	フルオラス担体およびそれを用いたオリゴヌクレオチド誘導体の製造方法
EP1904553A4 (de) *	2005-07-06	2013-04-24	Molly S Shoichet	Verfahren zur immobilisierung von biomolekülen auf polymeren unter verwendung von chemie vom click-typ
US8759508B2 (en)	2007-05-18	2014-06-24	Ge Healthcare Dharmacon, Inc.	Chromophoric silyl protecting groups and their use in the chemical synthesis of oligonucleotides
DE102010001992A1 (de)	2010-02-16	2011-08-18	Evonik Röhm GmbH, 64293	Funktionsmaterialien mit steuerbarer Viskosität
DE102010001987A1 (de)	2010-02-16	2011-08-18	Evonik Röhm GmbH, 64293	Funktionsmaterialien mit reversibler Vernetzung
JP5705512B2 (ja) *	2010-11-26	2015-04-22	農工大ティー・エル・オー株式会社	高分散性液相支持体を用いたオリゴヌクレオチド合成法
WO2015061246A1 (en) *	2013-10-21	2015-04-30	Isis Pharmaceuticals, Inc.	Method for solution phase detritylation of oligomeric compounds
KR101889893B1 (ko) *	2015-06-12	2018-08-22	애니젠 주식회사	선별적 용해도를 갖는 트리페닐메탄 유도체 및 그의 용도
US10947262B2 (en) *	2016-06-14	2021-03-16	Biogen Ma Inc.	Hydrophobic interaction chromatography for purification of oligonucleotides
HUE056740T2 (hu) *	2016-06-20	2022-03-28	Sekisui Medical Co Ltd	Új difenilmetán védõszer
US10981940B2 (en)	2016-11-11	2021-04-20	SEKISUl MEDICAL CO., LTD.	Trityl protecting agent
JP6322350B1 (ja) *	2016-11-11	2018-05-09	積水メディカル株式会社	新規トリチル保護剤
US10870667B2 (en) *	2017-12-19	2020-12-22	Sekisui Medical Co., Ltd.	Alkyldiphenylmethane protective agent
CA3199918A1 (en)	2021-01-05	2022-07-14	Randall Smith	Compositions including functional groups coupled to substrates, and methods of making the same
CN119462542B (zh) *	2024-06-24	2025-09-19	九江善水科技股份有限公司	一种三聚氯氰肟酯及其合成方法

Citations (7)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
SU627134A1 (ru) *	1977-06-16	1978-10-05	Предприятие П/Я М-5885	Способ получени тетрафурфурилоксисилана
WO1991011467A1 (en) *	1990-01-26	1991-08-08	Isp Investments Inc.	Alkenyl ethers and radiation curable compositions
WO1994022802A1 (en) *	1993-04-02	1994-10-13	Laporte Plc	Protecting group for use in oligodeoxyribonucleotide synthesis
EP0644196A1 (de) *	1993-09-17	1995-03-22	Ciba-Geigy Ag	TTTr als Schutzgruppe in der Nukleotidsynthese
WO1997014706A1 (en) *	1995-10-19	1997-04-24	Nexstar Pharmaceuticals, Inc.	Method for solution phase synthesis of oligonucleotides
WO1998030575A1 (en) *	1997-01-08	1998-07-16	Proligo Llc	Bioconjugation of macromolecules
WO1998030578A1 (en) *	1997-01-08	1998-07-16	Proligo Llc	Method for solution phase synthesis of oligonucleotides and peptides

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5580731A (en) *	1994-08-25	1996-12-03	Chiron Corporation	N-4 modified pyrimidine deoxynucleotides and oligonucleotide probes synthesized therewith
US5597909A (en) *	1994-08-25	1997-01-28	Chiron Corporation	Polynucleotide reagents containing modified deoxyribose moieties, and associated methods of synthesis and use

1998
- 1998-04-20 WO PCT/US1998/008192 patent/WO1998047910A1/en not_active Ceased
- 1998-04-20 JP JP54450398A patent/JP2001520660A/ja active Pending
- 1998-04-20 IL IL13237798A patent/IL132377A0/xx unknown
- 1998-04-20 EP EP98918629A patent/EP0979233A4/de not_active Withdrawn
- 1998-04-20 AU AU71520/98A patent/AU7152098A/en not_active Abandoned
- 1998-04-20 CA CA002286320A patent/CA2286320A1/en not_active Abandoned

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
SU627134A1 (ru) *	1977-06-16	1978-10-05	Предприятие П/Я М-5885	Способ получени тетрафурфурилоксисилана
WO1991011467A1 (en) *	1990-01-26	1991-08-08	Isp Investments Inc.	Alkenyl ethers and radiation curable compositions
WO1994022802A1 (en) *	1993-04-02	1994-10-13	Laporte Plc	Protecting group for use in oligodeoxyribonucleotide synthesis
EP0644196A1 (de) *	1993-09-17	1995-03-22	Ciba-Geigy Ag	TTTr als Schutzgruppe in der Nukleotidsynthese
WO1997014706A1 (en) *	1995-10-19	1997-04-24	Nexstar Pharmaceuticals, Inc.	Method for solution phase synthesis of oligonucleotides
WO1998030575A1 (en) *	1997-01-08	1998-07-16	Proligo Llc	Bioconjugation of macromolecules
WO1998030578A1 (en) *	1997-01-08	1998-07-16	Proligo Llc	Method for solution phase synthesis of oligonucleotides and peptides

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BATTEN, R.J. ET AL.: "Mew surface-coating materials: electron-beam curing of some silicone-containing acrylates", POLYMER, vol. 33, 1992, pages 3037 - 43, XP000941887 *
CHEMICAL ABSTRACTS, vol. 89, no. 25, 1978, Columbus, Ohio, US; abstract no. 215211, MUKHAMADALIEV, N. ET AL.: "Tetrafurfuryloxysilane" page 577; column l; XP002157922 *
H. SELIGER: "Scale-up of oligonucleotide synthesis in: Protocols for oligonucleotides and analogs (Editior: S. Agrawal)", METHODS IN MOLECULAR BIOLOGY, vol. 20, 1993, pages 391 - 403, XP002157921 *
See also references of WO9847910A1 *

Also Published As

Publication number	Publication date
CA2286320A1 (en)	1998-10-29
EP0979233A1 (de)	2000-02-16
WO1998047910A1 (en)	1998-10-29
IL132377A0 (en)	2001-03-19
JP2001520660A (ja)	2001-10-30
AU7152098A (en)	1998-11-13

Legal Events

Date	Code	Title	Description
1999-12-30	PUAI	Public reference made under article 153(3) epc to a published international application that has entered the european phase	Free format text: ORIGINAL CODE: 0009012
2000-02-16	17P	Request for examination filed	Effective date: 19991118
2000-02-16	AK	Designated contracting states	Kind code of ref document: A1 Designated state(s): AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
2000-04-12	RAP1	Party data changed (applicant data changed or rights of an application transferred)	Owner name: PROLIGO L.L.C.
2001-03-14	RIC1	Information provided on ipc code assigned before grant	Free format text: 7C 07H 19/00 A, 7C 07H 21/00 B, 7C 07H 21/02 B, 7C 07H 21/04 B, 7C 07H 19/10 B, 7C 07H 19/06 B, 7C 07H 19/16 B, 7C 07C 43/215 B, 7C 07C 43/225 B, 7C 07C 43/23 B, 7C 07F 7/02 B
2001-03-21	A4	Supplementary search report drawn up and despatched	Effective date: 20010206
2001-03-21	AK	Designated contracting states	Kind code of ref document: A4 Designated state(s): AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
2003-02-26	17Q	First examination report despatched	Effective date: 20030110
2003-05-09	STAA	Information on the status of an ep patent application or granted ep patent	Free format text: STATUS: THE APPLICATION HAS BEEN WITHDRAWN
2003-06-25	18W	Application withdrawn	Effective date: 20030429

Publication	Publication Date	Title
EP0979233A4 (de)	2001-03-21	Verfahren zur synthese von oligonukleotiden in lösung
EP1066308A4 (de)	2003-07-02	Verfahren zur synthese von oligonukleotidedn
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DE69715006D1 (de)	2002-10-02	Verfahren zur sequenzierung von nukleinsäuren
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DE59804785D1 (de)	2002-08-22	Verbessertes Verfahren zur Stabilisierung von Proteinen
ATE302792T1 (de)	2005-09-15	Verfahren zur schnellen synthese von peptiden in lösung
DE69621156D1 (de)	2002-06-13	Verfahren zur Hydrierung von Nitrilen
DE69824850D1 (de)	2004-08-05	Benzol-umwandlung in einem verbesserten verfahren zur ausreichung von kohlenwasserstoffen
DE69835345D1 (de)	2006-09-07	Verfahren zur kodierung von mehrwortinformation
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DE69627349D1 (de)	2003-05-15	Einrichtung zur synthese von oligonukleotiden
DE69709695D1 (de)	2002-02-21	Verfahren zur seitenzusammensetzung