EP0981535A4 - Procedes permettant de produire et de purifier les molecules d'acide nucleique - Google Patents

Procedes permettant de produire et de purifier les molecules d'acide nucleique

Info

Publication number
EP0981535A4
EP0981535A4 EP98922202A EP98922202A EP0981535A4 EP 0981535 A4 EP0981535 A4 EP 0981535A4 EP 98922202 A EP98922202 A EP 98922202A EP 98922202 A EP98922202 A EP 98922202A EP 0981535 A4 EP0981535 A4 EP 0981535A4
Authority
EP
European Patent Office
Prior art keywords
purification
production
nucleic acid
acid molecules
molecules
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP98922202A
Other languages
German (de)
English (en)
Other versions
EP0981535A1 (fr
Inventor
Christian E Gruber
Joel A Jessee
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Inc
Original Assignee
Life Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Inc filed Critical Life Technologies Inc
Publication of EP0981535A1 publication Critical patent/EP0981535A1/fr
Publication of EP0981535A4 publication Critical patent/EP0981535A4/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
EP98922202A 1997-05-12 1998-05-12 Procedes permettant de produire et de purifier les molecules d'acide nucleique Withdrawn EP0981535A4 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US4621997P 1997-05-12 1997-05-12
US46219P 1997-05-12
PCT/US1998/009586 WO1998051699A1 (fr) 1997-05-12 1998-05-12 Procedes permettant de produire et de purifier les molecules d'acide nucleique
CA002287525A CA2287525A1 (fr) 1997-05-12 1999-11-12 Procede de fabrication et de purification des molecules de l'acide nucleique

Publications (2)

Publication Number Publication Date
EP0981535A1 EP0981535A1 (fr) 2000-03-01
EP0981535A4 true EP0981535A4 (fr) 2000-11-29

Family

ID=25681276

Family Applications (1)

Application Number Title Priority Date Filing Date
EP98922202A Withdrawn EP0981535A4 (fr) 1997-05-12 1998-05-12 Procedes permettant de produire et de purifier les molecules d'acide nucleique

Country Status (4)

Country Link
EP (1) EP0981535A4 (fr)
JP (1) JP2002502237A (fr)
CA (1) CA2287525A1 (fr)
WO (1) WO1998051699A1 (fr)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7122304B2 (en) * 1997-05-12 2006-10-17 Whatman, Inc. Methods for the storage and synthesis of nucleic acids using a solid support
US7074556B2 (en) 1999-03-02 2006-07-11 Invitrogen Corporation cDNA synthesis improvements
US6083727A (en) * 1999-06-30 2000-07-04 Incyte Pharmaceuticals Inc. Methods and compositions for producing 5' enriched cDNA libraries
WO2001066802A1 (fr) * 2000-03-09 2001-09-13 Genetag Technology, Inc. Systemes et procedes de quantification et amplification a la fois d'elements de signalisation et de sondes, dans des puces d'adnc et des matrices de micro-echantillons d'expression
CN1430670A (zh) 2000-05-26 2003-07-16 茵维特罗根公司 热稳定逆转录酶及其用途
US7078208B2 (en) 2000-05-26 2006-07-18 Invitrogen Corporation Thermostable reverse transcriptases and uses thereof
DE10120798B4 (de) * 2001-04-27 2005-12-29 Genovoxx Gmbh Verfahren zur Bestimmung der Genexpression
WO2004024749A2 (fr) 2002-09-13 2004-03-25 Invitrogen Corporation Transcriptases inverses thermostables et leurs utilisations
WO2006019892A2 (fr) * 2004-07-14 2006-02-23 Invitrogen Corporation Procedes et systemes de conception experimentale in silico et de fourniture d'un produit biotechnologique a un client
EP2581447A1 (fr) * 2011-10-12 2013-04-17 Royal College of Surgeons in Ireland Isolation sélective d'une molécule ARN messager dotée de ses molécules micro-ARN parentes liées
JP2014176303A (ja) * 2013-03-13 2014-09-25 Seiko Epson Corp ｃＤＮＡの合成方法

Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989012063A1 (fr) * 1988-06-01 1989-12-14 The United States Of America, As Represented By Th Sequençage d'adn; modification de la reaction en chaine de polymerase
WO1990006044A2 (fr) * 1988-11-21 1990-06-14 Dynal As PROCEDE DE PRODUCTION D'ADNc
WO1991011533A1 (fr) * 1990-01-26 1991-08-08 E.I. Du Pont De Nemours And Company Procede d'isolement de produits d'extension a partir de reactions de polymerase d'amorces d'adn orientees a l'aide d'un brin complementaire
WO1991018114A1 (fr) * 1990-05-22 1991-11-28 Medical Research Council Amplification de polynucleotides
EP0622457A1 (fr) * 1992-07-21 1994-11-02 Sumitomo Electric Industries, Ltd. PROCEDE DE SEPARATION D'ADNc A SPECIFICITE CELLULAIRE, ET PREPARATION D'UNE BANQUE DE CES ADNc
EP0645449A1 (fr) * 1993-09-24 1995-03-29 Roche Diagnostics GmbH Méthode pour le clonage spécifique d'acides nucléiques
WO1996012014A1 (fr) * 1994-10-13 1996-04-25 Lynx Therapeutics, Inc. Systeme de marquage moleculaire
EP0735144A1 (fr) * 1995-03-28 1996-10-02 Research Development Corporation Of Japan Procédé pour l'indexation (la classification) moléculaire de gènes utilisant des enzymes de restriction
EP0761822A2 (fr) * 1995-09-12 1997-03-12 The Johns Hopkins University School Of Medicine Méthode d'analyse sérielle de l'expression de gènes
WO1998014619A1 (fr) * 1996-10-03 1998-04-09 Incyte Pharmaceuticals, Inc. Procedes de generation et d'analyse de marqueurs de transcrits
EP0872559A1 (fr) * 1995-11-09 1998-10-21 Shionogi & Co., Ltd. Procede de detection simultanee de polygenes

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5726022A (en) * 1996-11-18 1998-03-10 Lifespan Biosciences, Inc. Subtractive hybridization and capture methods and kits for differential isolation of nucleic acids including disease-associated sequences

Patent Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989012063A1 (fr) * 1988-06-01 1989-12-14 The United States Of America, As Represented By Th Sequençage d'adn; modification de la reaction en chaine de polymerase
WO1990006044A2 (fr) * 1988-11-21 1990-06-14 Dynal As PROCEDE DE PRODUCTION D'ADNc
WO1991011533A1 (fr) * 1990-01-26 1991-08-08 E.I. Du Pont De Nemours And Company Procede d'isolement de produits d'extension a partir de reactions de polymerase d'amorces d'adn orientees a l'aide d'un brin complementaire
WO1991018114A1 (fr) * 1990-05-22 1991-11-28 Medical Research Council Amplification de polynucleotides
EP0622457A1 (fr) * 1992-07-21 1994-11-02 Sumitomo Electric Industries, Ltd. PROCEDE DE SEPARATION D'ADNc A SPECIFICITE CELLULAIRE, ET PREPARATION D'UNE BANQUE DE CES ADNc
EP0645449A1 (fr) * 1993-09-24 1995-03-29 Roche Diagnostics GmbH Méthode pour le clonage spécifique d'acides nucléiques
WO1996012014A1 (fr) * 1994-10-13 1996-04-25 Lynx Therapeutics, Inc. Systeme de marquage moleculaire
EP0735144A1 (fr) * 1995-03-28 1996-10-02 Research Development Corporation Of Japan Procédé pour l'indexation (la classification) moléculaire de gènes utilisant des enzymes de restriction
EP0761822A2 (fr) * 1995-09-12 1997-03-12 The Johns Hopkins University School Of Medicine Méthode d'analyse sérielle de l'expression de gènes
EP0872559A1 (fr) * 1995-11-09 1998-10-21 Shionogi & Co., Ltd. Procede de detection simultanee de polygenes
WO1998014619A1 (fr) * 1996-10-03 1998-04-09 Incyte Pharmaceuticals, Inc. Procedes de generation et d'analyse de marqueurs de transcrits

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of WO9851699A1 *

Also Published As

Publication number Publication date
EP0981535A1 (fr) 2000-03-01
CA2287525A1 (fr) 2001-05-12
JP2002502237A (ja) 2002-01-22
WO1998051699A1 (fr) 1998-11-19

Similar Documents

Publication Publication Date Title
DE59912604D1 (de) Verfahren zur isolierung und aufreinigung von nucleinsäuren
DE69801797D1 (de) Verfahren zur Herstellung von Acrylsäure
DE69943292D1 (de) Verfahren zur Amplifikation und Sequenzierung von Nucleinsäuren und zur Herstellung von Nucleinsäuren mit verminderter thermodynamischer Stabilität
DE69928683D1 (de) Verfahren zur amplifikation und zur sequenzierung von nukleinsäuren
DE69902139D1 (de) Verfahren zur Herstellung von Salpetersäure
DE69702354D1 (de) Verfahren zur Herstellung von Acrylsäure
DE69704964D1 (de) Verfahren zur Herstellung von Acryloxypropylsilane
DE69506496D1 (de) Verfahren zur Herstellung von modifizierten Harzen und ihre Verwendungen
DE69505336D1 (de) Verfahren zur Herstellung von Acrylsäure
DE69817476D1 (de) Verfahren zur Herstellung von modifiziertem Acrylkautschuk
DE69621281D1 (de) Acryl-Säure-Derivate, Verfahren zur Herstellung von Acryl-Säure-Derivaten und Acryl-Säure-Polymere
DE69802534D1 (de) Verfahren zur herstellung von fluorkohlenwasserstoffen
DE59707913D1 (de) Kontinuierliches Verfahren zur Herstellung von gamma- Aminopropyltrialkoxysilanen
DE69807831D1 (de) Verfahren zur Reinigung von Bisphenolen und Herstellung von Polycarbonaten daraus
DE69815098D1 (de) Verfahren zur herstellung von carbonsäureamiden
DE69502313D1 (de) Verfahren zur biologischen Reinigung von Gasströmen
DE69903698D1 (de) Verfahren zur Herstellung von Acrylsäure
DE69909181D1 (de) Verfahren zur Herstellung von Acrolein und Acrylsäure
EP0981535A4 (fr) Procedes permettant de produire et de purifier les molecules d'acide nucleique
DE69727977D1 (de) Verfahren zur simultanen herstellung von acrylnitril und acrylsäure
DE69712157D1 (de) Verfahren zur Herstellung von Acrylnitril
DE69630038D1 (de) Verfahren zur herstellung und aufreinigung von enzymen
DE59705573D1 (de) Verfahren zur katalytischen herstellung von n-acylglycinderivaten
DE69637616D1 (de) Verfahren zur herstellung von nukleinsäuren
DE59712415D1 (de) Verfahren zur amplifikation von nukleinsäure

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 19991018

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

A4 Supplementary search report drawn up and despatched

Effective date: 20001016

AK Designated contracting states

Kind code of ref document: A4

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

RIC1 Information provided on ipc code assigned before grant

Free format text: 7C 12N 15/10 A, 7C 12Q 1/68 B

17Q First examination report despatched

Effective date: 20021217

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20041110