EP1027076A2 - Utilisation de vecteurs tels que adenovirus et/ou virus adeno-associes et/ou retrovirus et/ou herpesvirus et/ou liposomes et/ou plasmides comme vehicules d'informations genetiques relatives a l'aptitude des cellules de mammiferes a produire des agents pour le traitement de pathologies osseuses - Google Patents

Utilisation de vecteurs tels que adenovirus et/ou virus adeno-associes et/ou retrovirus et/ou herpesvirus et/ou liposomes et/ou plasmides comme vehicules d'informations genetiques relatives a l'aptitude des cellules de mammiferes a produire des agents pour le traitement de pathologies osseuses

Info

Publication number
EP1027076A2
EP1027076A2 EP98959810A EP98959810A EP1027076A2 EP 1027076 A2 EP1027076 A2 EP 1027076A2 EP 98959810 A EP98959810 A EP 98959810A EP 98959810 A EP98959810 A EP 98959810A EP 1027076 A2 EP1027076 A2 EP 1027076A2
Authority
EP
European Patent Office
Prior art keywords
bone
vectors
cells
use according
viruses
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
EP98959810A
Other languages
German (de)
English (en)
Inventor
Axel Wilhelm August Baltzer
Christian Lattermann
Janey Desales Whalen
Paul David Robbins
Christopher Howard Evans
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Pittsburgh
Original Assignee
University of Pittsburgh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE1997147718 external-priority patent/DE19747718A1/de
Priority claimed from DE1997147719 external-priority patent/DE19747719A1/de
Application filed by University of Pittsburgh filed Critical University of Pittsburgh
Publication of EP1027076A2 publication Critical patent/EP1027076A2/fr
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/19—Cytokines; Lymphokines; Interferons
    • A61K38/20—Interleukins [IL]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy

Definitions

  • vectors such as adenoviruses and / or adeno-associated viruses and / or retroviruses and / or herpes simplex viruses and / or liposomes and / or piasmids as vehicles for genetic information to enable mammalian cells to produce agents for the treatment of bone pathogens.
  • the invention relates to the use of adenoviruses and / or adeno-associated viruses and / or retroviruses and / or herpes simplex viruses and / or liposomes and / or piasmids as vectors, respective vehicles for genetic information to enable mammalian cells to be therapeutically enabled, to produce proteins, effective as medicinal products, for correcting bone pathologies at the cellular level.
  • the enzyme activity of the luciferase after intraosseous vector application of Ad-Luc is shown in FIG. 4. It demonstrates that transgene expression persists in the bones and muscles over the entire course of the examination interval of 21 days. Transgene expression from the liver (2 days) and draining lymph nodes (14 days) is transient and does not reach the level of enzyme activity in the area of the injection.
  • luciferase activity was found in the bone and bone marrow, as well as in the defect-filling connective tissue and in the surrounding muscle tissue at the injection site. 5 days after the transduction there was a slight luciferase activity in the liver which disappeared completely after 10 days. No luciferase activity could be found in the contralateral femur, the lungs and the spleen (Fig. 8).

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Virology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Physics & Mathematics (AREA)
  • Epidemiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Public Health (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

L'invention concerne l'utilisation de vecteurs tels que adénovirus et/ou virus-adéno associés et/ou rétrovirus et/ou herpèsvirus et/ou liposomes et/ou plasmides, comme agents stimulant les cellules de mammifères en vue de la production de protéines thérapeutiques, agissant comme médicaments, au niveau cellulaire, pour le traitement génétique de pathologies osseuses.
EP98959810A 1997-10-29 1998-10-29 Utilisation de vecteurs tels que adenovirus et/ou virus adeno-associes et/ou retrovirus et/ou herpesvirus et/ou liposomes et/ou plasmides comme vehicules d'informations genetiques relatives a l'aptitude des cellules de mammiferes a produire des agents pour le traitement de pathologies osseuses Ceased EP1027076A2 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
DE1997147718 DE19747718A1 (de) 1997-10-29 1997-10-29 Verfahren zur Therapie knochenresorbierender Erkrankungen mit der molekularbiologischen Methode der Gentherapie
DE19747719 1997-10-29
DE19747718 1997-10-29
DE1997147719 DE19747719A1 (de) 1997-10-29 1997-10-29 Verfahren zur Verbesserung der Knochenheilung mit der molekularbiologischen Methode der Genübertragung
PCT/EP1998/006849 WO1999021589A2 (fr) 1997-10-29 1998-10-29 Utilisation de vecteurs tels que adenovirus et/ou virus adeno-associes et/ou retrovirus et/ou herpesvirus et/ou liposomes et/ou plasmides comme vehicules d'informations genetiques relatives a l'aptitude des cellules de mammiferes a produire des agents pour le traitement de pathologies osseuses

Publications (1)

Publication Number Publication Date
EP1027076A2 true EP1027076A2 (fr) 2000-08-16

Family

ID=26041170

Family Applications (1)

Application Number Title Priority Date Filing Date
EP98959810A Ceased EP1027076A2 (fr) 1997-10-29 1998-10-29 Utilisation de vecteurs tels que adenovirus et/ou virus adeno-associes et/ou retrovirus et/ou herpesvirus et/ou liposomes et/ou plasmides comme vehicules d'informations genetiques relatives a l'aptitude des cellules de mammiferes a produire des agents pour le traitement de pathologies osseuses

Country Status (5)

Country Link
US (1) US7105494B1 (fr)
EP (1) EP1027076A2 (fr)
AU (1) AU750803B2 (fr)
CA (1) CA2308511C (fr)
WO (1) WO1999021589A2 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2308511C (fr) 1997-10-29 2016-01-19 University Of Pittsburgh Of The Commonwealth System Of Higher Education Utilisation de cellules de mammiferes transfectees ou transduites pour traiter des maladies osseuses
WO2001082973A2 (fr) * 2000-04-28 2001-11-08 University Of Pittsburgh Of The Commonwealth System Of Higher Education Vecteurs viraux et non viraux en tant que vehicules d'administration de transgenes pour le traitement de pathologies osseuses
US6579522B1 (en) 2000-06-27 2003-06-17 Genvec, Inc. Replication deficient adenoviral TNF vector
WO2002055656A2 (fr) * 2000-11-08 2002-07-18 Tissuegene Inc THERAPIE GENIQUE UTILISANT UN TGF-$G(b)
DE60143964D1 (de) * 2001-02-06 2011-03-10 Sydney Children S Hospitals Network Randwick And Westmead Incorporating The Royal Alexandra Hospital Arzneimittel zur behandlung von osteonekrose und zur versorgung von patienten mit risiko für die entstehung von osteonekrose
US20030086903A1 (en) 2001-11-02 2003-05-08 Genvec, Inc. Therapeutic regimen for treating cancer
JP2009534394A (ja) * 2006-04-21 2009-09-24 ザ・ユニヴァーシティ・オヴ・ノース・キャロライナ・アト・チャペル・ヒル 結合組織障害の治療
US8138160B2 (en) * 2006-08-03 2012-03-20 Warsaw Orthopedic, Inc. Reagents, methods and systems to suppress pro-inflammatory cytokines
WO2018125970A1 (fr) * 2016-12-30 2018-07-05 Salk Institute For Biological Studies Adénovirus synthétiques ciblant un tissu osseux et utilisations correspondantes
US12478661B2 (en) 2017-10-03 2025-11-25 The Johns Hopkins University Lung cell treatments to prevent or treat disease

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HK1008049A1 (en) 1987-09-11 1999-04-30 Whitehead Institute For Biomedical Research Transduced fibroblasts and use therefor
US6090790A (en) * 1989-12-14 2000-07-18 Eriksson; Elof Gene delivery by microneedle injection
US5670488A (en) 1992-12-03 1997-09-23 Genzyme Corporation Adenovirus vector for gene therapy
US5168050A (en) * 1990-05-24 1992-12-01 Genentech, Inc. Mammalian expression of the bone morphogenetic protein-2b using bmp2a/bmp2b fusion
US5618531A (en) 1990-10-19 1997-04-08 New York University Method for increasing the viability of cells which are administered to the brain or spinal cord
DE69123981T3 (de) 1990-10-31 2006-06-14 Cell Genesys Inc Nützliche retrovirale vektoren für die gentherapie
US5858355A (en) 1990-12-20 1999-01-12 University Of Pittsburgh Of The Commonwealth System Of Higher Education IRAP gene as treatment for arthritis
US5674844A (en) 1991-03-11 1997-10-07 Creative Biomolecules, Inc. Treatment to prevent loss of and/or increase bone mass in metabolic bone diseases
US5789543A (en) 1993-12-30 1998-08-04 President And Fellows Of Harvard College Vertebrate embryonic pattern-inducing proteins and uses related thereto
DE4339352A1 (de) 1993-11-18 1995-05-24 Univ Ludwigs Albert Verfahren zur Markierung von Zellen, mit diesem Verfahren markierte Zellen, Verwendung des Wildtyp-Adeno-assoziierten Virus zur Markierung von Zellen und Testkits zum Nachweis des Wildtyp-Adeno-assoziierten Virus in Zellen
US5942496A (en) 1994-02-18 1999-08-24 The Regent Of The University Of Michigan Methods and compositions for multiple gene transfer into bone cells
US5763416A (en) 1994-02-18 1998-06-09 The Regent Of The University Of Michigan Gene transfer into bone cells and tissues
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Also Published As

Publication number Publication date
CA2308511A1 (fr) 1999-05-06
AU1557999A (en) 1999-05-17
US7105494B1 (en) 2006-09-12
WO1999021589A2 (fr) 1999-05-06
CA2308511C (fr) 2016-01-19
WO1999021589A3 (fr) 1999-10-14
AU750803B2 (en) 2002-07-25

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