EP1051499A2 - Polypeptides et polynucleotides de tyrosylproteine-sulfotransferases - Google Patents

Polypeptides et polynucleotides de tyrosylproteine-sulfotransferases

Info

Publication number
EP1051499A2
EP1051499A2 EP99904344A EP99904344A EP1051499A2 EP 1051499 A2 EP1051499 A2 EP 1051499A2 EP 99904344 A EP99904344 A EP 99904344A EP 99904344 A EP99904344 A EP 99904344A EP 1051499 A2 EP1051499 A2 EP 1051499A2
Authority
EP
European Patent Office
Prior art keywords
seq
polynucleotide
tpst
bases
tyrosylprotein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP99904344A
Other languages
German (de)
English (en)
Inventor
Kevin L. Moore
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Oklahoma
Original Assignee
University of Oklahoma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/150,133 external-priority patent/US6060295A/en
Priority claimed from US09/150,141 external-priority patent/US6071732A/en
Application filed by University of Oklahoma filed Critical University of Oklahoma
Publication of EP1051499A2 publication Critical patent/EP1051499A2/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/13—Transferases (2.) transferring sulfur containing groups (2.8)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70596—Molecules with a "CD"-designation not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against enzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes

Definitions

  • Antibodies generated against the polypeptides corresponding to a sequence of the present invention can be obtained by direct injection of the polypeptides into an animal or by other appropriate forms of administering the polypeptides to an animal, preferably a nonhuman. The antibody so obtained will then bind the polypeptides itself. In this manner, even a sequence encoding only a fragment of the polypeptides can be used to generate antibodies binding the whole native polypeptides. Such antibodies can then be used to isolate the polypeptide from tissue expressing that polypeptide.
  • Bound protein was eluted with 10 mM EDTA, 0.15 M NaCl, 20 mM MOPS (pH 7.5), 0.1% Triton X-100. Fractions were assayed for protein content and TPST activity. Samples were electrophoresed on 10% SDS polyacrylamide gels and proteins transferred to Hybond-P membranes (Amersham) . The membranes were blocked, probed with HPC4, and bound antibody detected with enhanced chemiluminescence using horseradish peroxidase-conjugated anti-mouse immunoglobulin (Amersham) .
  • Base homologies of the various TPST gene core sequences are shown in Table VIII.
  • the core sequences of human and mouse TPST-1 have 90% homology (identity) .
  • Human and mouse TPST-2 gene core sequences have 90% homology.
  • Human TPST-A and TPST-B gene core sequences have 59% homology.
  • Human TPST-1 and TPST-2 gene core sequences have 68% homology.
  • Mouse TPST-1 and TPST-2 gene core sequences have 69% homology.
  • Human TPST-1 and mouse TPST-2 gene core sequences have 67% homology.
  • Human TPST-2 and mouse TPST-1 gene core sequences have 67% homology.

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Hematology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

L'invention concerne des tyrosylprotéine-sulfotransférases et des acides nucléiques codant ces tyrosylprotéine-sulfotransférases. On a identifié chez l'homme, la souris, et C. elegans des isotopes doubles de l'enzyme et des acides nucléiques codant ces enzymes. Ces polypeptides et polynucléotides présentent une gamme étendue d'homologies. Les polynucléotides peuvent être utilisés pour transformer ou transfecter des cellules hôtes afin de produire des formes sensiblement pures de l'enzyme, ou dans un système d'expression destiné à la sulfatation post-translationnelle de la tyrosine de protéines et de peptides produits dans ce système d'expression.
EP99904344A 1998-01-29 1999-01-28 Polypeptides et polynucleotides de tyrosylproteine-sulfotransferases Withdrawn EP1051499A2 (fr)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US7299498P 1998-01-29 1998-01-29
US72994P 1998-01-29
US09/150,133 US6060295A (en) 1998-01-29 1998-09-09 Nucleic acids and expression systems encoding tyrosylprotein sulfo transferases
US09/150,141 US6071732A (en) 1998-01-29 1998-09-09 Tyrosylprotein sulfotransferases, nucleic acids encoding tyrosylprotein sulfotransferases, and methods of use thereof
US150141 1998-09-09
US150133 1998-09-09
PCT/US1999/001756 WO1999038980A2 (fr) 1998-01-29 1999-01-28 Polypeptides et polynucleotides de tyrosylproteine-sulfotransferases

Publications (1)

Publication Number Publication Date
EP1051499A2 true EP1051499A2 (fr) 2000-11-15

Family

ID=27372205

Family Applications (1)

Application Number Title Priority Date Filing Date
EP99904344A Withdrawn EP1051499A2 (fr) 1998-01-29 1999-01-28 Polypeptides et polynucleotides de tyrosylproteine-sulfotransferases

Country Status (3)

Country Link
EP (1) EP1051499A2 (fr)
AU (1) AU2475899A (fr)
WO (1) WO1999038980A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0008326D0 (en) * 2000-04-06 2000-05-24 Shs International Ltd Diagnosis and treatment of autism and related disorders
US7306923B2 (en) 2004-06-14 2007-12-11 Galapagos N.V. Methods for identification, and compounds useful for the treatment of degenerative & inflammatory diseases

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO9938980A3 *

Also Published As

Publication number Publication date
WO1999038980A9 (fr) 1999-10-21
AU2475899A (en) 1999-08-16
WO1999038980A3 (fr) 2000-03-30
WO1999038980A2 (fr) 1999-08-05

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