EP1115734A4 - METHODS FOR MEASURING PROTEIN-PROTEIN INTERACTIONS IN LIVING CELLS - Google Patents

METHODS FOR MEASURING PROTEIN-PROTEIN INTERACTIONS IN LIVING CELLS

Info

Publication number
EP1115734A4
EP1115734A4 EP99942401A EP99942401A EP1115734A4 EP 1115734 A4 EP1115734 A4 EP 1115734A4 EP 99942401 A EP99942401 A EP 99942401A EP 99942401 A EP99942401 A EP 99942401A EP 1115734 A4 EP1115734 A4 EP 1115734A4
Authority
EP
European Patent Office
Prior art keywords
protein
methods
living cells
measuring
interactions
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP99942401A
Other languages
German (de)
French (fr)
Other versions
EP1115734A1 (en
Inventor
Tobias Meyer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Duke University
Original Assignee
Duke University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Duke University filed Critical Duke University
Publication of EP1115734A1 publication Critical patent/EP1115734A1/en
Publication of EP1115734A4 publication Critical patent/EP1115734A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
EP99942401A 1998-09-24 1999-08-24 METHODS FOR MEASURING PROTEIN-PROTEIN INTERACTIONS IN LIVING CELLS Withdrawn EP1115734A4 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US10163798P 1998-09-24 1998-09-24
US101637P 1998-09-24
PCT/US1999/019118 WO2000017221A1 (en) 1998-09-24 1999-08-24 Method of measuring protein-protein interactions in living cells

Publications (2)

Publication Number Publication Date
EP1115734A1 EP1115734A1 (en) 2001-07-18
EP1115734A4 true EP1115734A4 (en) 2004-07-21

Family

ID=22285675

Family Applications (1)

Application Number Title Priority Date Filing Date
EP99942401A Withdrawn EP1115734A4 (en) 1998-09-24 1999-08-24 METHODS FOR MEASURING PROTEIN-PROTEIN INTERACTIONS IN LIVING CELLS

Country Status (5)

Country Link
EP (1) EP1115734A4 (en)
JP (1) JP2002526756A (en)
AU (1) AU5578999A (en)
CA (1) CA2345392A1 (en)
WO (1) WO2000017221A1 (en)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1198586B1 (en) * 1999-06-26 2010-03-03 Odyssey Thera, Inc. An in vivo library-versus-library selection of optimized protein-protein interactions
CA2414626A1 (en) 2000-07-04 2002-01-10 Bioimage A/S A method for extracting quantitative information relating to interactions between cellular components
WO2003029827A2 (en) * 2001-10-01 2003-04-10 Bioimage A/S A method of detecting intracellular protein-protein interactions using three heterologous conjugates
US20050214734A1 (en) * 2002-04-01 2005-09-29 Seishi Kato Cell population provided with identification codes and method of screening cell population
US7771952B2 (en) 2002-06-26 2010-08-10 Abott Laboratories Modulators and modulation of the interaction between RGM and Neogenin
JP2004085528A (en) * 2002-07-05 2004-03-18 Kansai Tlo Kk Method and apparatus for detecting protein association by change in diffusion constant
EP2053409A1 (en) 2003-11-20 2009-04-29 F. Hoffmann-La Roche Ag Specific markers for metabolic syndrome
CN101277974A (en) 2005-09-30 2008-10-01 阿伯特有限及两合公司 Binding domains of proteins of the Repulsive Guide Molecule (RGM) protein family, functional fragments thereof and their uses
US8962803B2 (en) 2008-02-29 2015-02-24 AbbVie Deutschland GmbH & Co. KG Antibodies against the RGM A protein and uses thereof
KR100948767B1 (en) * 2008-11-12 2010-03-23 한국기초과학지원연구원 Method for detecting interactions between two and more biological macromolecules
KR20120118002A (en) 2009-12-08 2012-10-25 애보트 게엠베하 운트 콤파니 카게 Monoclonal antibodies against the rgm a protein for use in the treatment of retinal nerve fiber layer degeneration
WO2011083147A1 (en) 2010-01-08 2011-07-14 Cemm-Forschungsinstitut Für Molekulare Medizin Gmbh Wave1 inhibition in the medical intervention of inflammatory diseases and/or infections caused by a pathogen
EP2561068A1 (en) 2010-04-19 2013-02-27 Medizinische Universität Innsbruck Tmem195 encodes for tetrahydrobiopterin-dependent alkylglycerol monooxygenase activity
CN104105964B (en) 2011-12-05 2016-08-24 株式会社医学生物学研究所 Methods for the detection of protein-protein interactions
EP3369746A1 (en) 2012-01-27 2018-09-05 AbbVie Deutschland GmbH & Co KG Composition and method for diagnosis and treatment of diseases associated with neurite degeneration
EP2823044B1 (en) 2012-03-06 2017-12-06 VIB vzw Membrane span-kinase fusion protein and the uses thereof
WO2015190529A1 (en) 2014-06-10 2015-12-17 株式会社医学生物学研究所 Method for judging protein interaction
US10591486B2 (en) 2014-07-23 2020-03-17 Institut National De La Sante Et De La Recherche Medicale (Inserm) Methods and tools for detecting interactions in eukaryotic cells using microtubule structures and dynamics
KR101823990B1 (en) * 2016-09-07 2018-01-31 포항공과대학교 산학협력단 A Method for Quantitative Analysis of Transient Interactions of Membrane Proteins in a Single Living Cell

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996023898A1 (en) * 1995-01-31 1996-08-08 Novo Nordisk A/S A method of detecting biologically active substances
WO1998002571A1 (en) * 1996-07-16 1998-01-22 The Regents Of The University Of California Assays for protein kinases using fluorescent protein substrates
WO1998034120A1 (en) * 1997-01-31 1998-08-06 Universite De Montreal Protein fragment complementation assays to detect biomolecular interactions

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5804604A (en) * 1989-12-21 1998-09-08 Biogen, Inc. Tat-derived transport polypeptides and fusion proteins
US5427908A (en) * 1990-05-01 1995-06-27 Affymax Technologies N.V. Recombinant library screening methods
US5871907A (en) * 1991-05-15 1999-02-16 Medical Research Council Methods for producing members of specific binding pairs
AU689755B2 (en) * 1992-09-22 1998-04-09 Biofocus Discovery Limited Recombinant viruses displaying a nonviral polypeptide on their external surface

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996023898A1 (en) * 1995-01-31 1996-08-08 Novo Nordisk A/S A method of detecting biologically active substances
WO1998002571A1 (en) * 1996-07-16 1998-01-22 The Regents Of The University Of California Assays for protein kinases using fluorescent protein substrates
WO1998034120A1 (en) * 1997-01-31 1998-08-06 Universite De Montreal Protein fragment complementation assays to detect biomolecular interactions

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BRODER Y C ET AL: "The ras recruitment system, a novel approach to the study of protein-protein interactions", CURRENT BIOLOGY, CURRENT SCIENCE,, GB, vol. 8, no. 20, 8 October 1998 (1998-10-08), pages 1121 - 1124, XP002135902, ISSN: 0960-9822 *
MAROUN M ET AL: "A NOVEL IN VIVO ASSAY FOR THE ANALYSIS OF PROTEIN-PROTEIN INTERACTION", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 27, no. 13, 1999, pages COMPLETE, XP000904543, ISSN: 0305-1048 *
SAKAI ET AL: "Translocation of protein kinase C-gamma and epsilon - Direct visualization in living cells using fusion protein with green fluorescent protein", THE JOURNAL OF CELL BIOLOGY, ROCKEFELLER UNIVERSITY PRESS, US, 1997, XP002078902, ISSN: 0021-9525 *
See also references of WO0017221A1 *

Also Published As

Publication number Publication date
WO2000017221A9 (en) 2000-06-29
WO2000017221A1 (en) 2000-03-30
JP2002526756A (en) 2002-08-20
AU5578999A (en) 2000-04-10
CA2345392A1 (en) 2000-03-30
EP1115734A1 (en) 2001-07-18

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