EP1115734A4 - Procede de mesure des interactions proteine-proteine dans des cellules vivantes - Google Patents

Procede de mesure des interactions proteine-proteine dans des cellules vivantes

Info

Publication number
EP1115734A4
EP1115734A4 EP99942401A EP99942401A EP1115734A4 EP 1115734 A4 EP1115734 A4 EP 1115734A4 EP 99942401 A EP99942401 A EP 99942401A EP 99942401 A EP99942401 A EP 99942401A EP 1115734 A4 EP1115734 A4 EP 1115734A4
Authority
EP
European Patent Office
Prior art keywords
protein
methods
living cells
measuring
interactions
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP99942401A
Other languages
German (de)
English (en)
Other versions
EP1115734A1 (fr
Inventor
Tobias Meyer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Duke University
Original Assignee
Duke University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Duke University filed Critical Duke University
Publication of EP1115734A1 publication Critical patent/EP1115734A1/fr
Publication of EP1115734A4 publication Critical patent/EP1115734A4/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
EP99942401A 1998-09-24 1999-08-24 Procede de mesure des interactions proteine-proteine dans des cellules vivantes Withdrawn EP1115734A4 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US10163798P 1998-09-24 1998-09-24
US101637P 1998-09-24
PCT/US1999/019118 WO2000017221A1 (fr) 1998-09-24 1999-08-24 Procede de mesure des interactions proteine-proteine dans des cellules vivantes

Publications (2)

Publication Number Publication Date
EP1115734A1 EP1115734A1 (fr) 2001-07-18
EP1115734A4 true EP1115734A4 (fr) 2004-07-21

Family

ID=22285675

Family Applications (1)

Application Number Title Priority Date Filing Date
EP99942401A Withdrawn EP1115734A4 (fr) 1998-09-24 1999-08-24 Procede de mesure des interactions proteine-proteine dans des cellules vivantes

Country Status (5)

Country Link
EP (1) EP1115734A4 (fr)
JP (1) JP2002526756A (fr)
AU (1) AU5578999A (fr)
CA (1) CA2345392A1 (fr)
WO (1) WO2000017221A1 (fr)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001000866A1 (fr) * 1999-06-26 2001-01-04 Odyssey Pharmaceuticals, Inc. Selection in vivo bibliotheque contre bibliotheque d'interactions proteine-proteine optimisees
CA2414626A1 (fr) * 2000-07-04 2002-01-10 Bioimage A/S Technique permettant d'extraire des informations quantitatives relatives aux interactions entre des composants cellulaires
WO2003029827A2 (fr) * 2001-10-01 2003-04-10 Bioimage A/S Methode amelioree permettant de detecter des interactions entre des constituants cellulaires presents dans des cellules vivantes intactes et d'extraire des informations quantitatives se rapportant auxdites interactions par nouvelle repartition de fluorescence
US20050214734A1 (en) * 2002-04-01 2005-09-29 Seishi Kato Cell population provided with identification codes and method of screening cell population
WO2004003150A2 (fr) 2002-06-26 2004-01-08 Yale University Modulateurs et modulation de l'interaction entre les rgm et la neogenine
JP2004085528A (ja) * 2002-07-05 2004-03-18 Kansai Tlo Kk 拡散定数変化による蛋白質の会合検出方法及び装置
DE602004020855D1 (de) 2003-11-20 2009-06-10 Hoffmann La Roche Spezifische Marker für Stoffwechselssyndrome
EP1928905B1 (fr) 2005-09-30 2015-04-15 AbbVie Deutschland GmbH & Co KG Domaines de liaison de proteines de la famille proteinique des molecules de guidage repulsif (rgm), fragments fonctionnels de ces domaines et leur utilisation
US8962803B2 (en) 2008-02-29 2015-02-24 AbbVie Deutschland GmbH & Co. KG Antibodies against the RGM A protein and uses thereof
KR100948767B1 (ko) * 2008-11-12 2010-03-23 한국기초과학지원연구원 생체 고분자 물질의 상호작용 검출 방법
MX2012006560A (es) 2009-12-08 2012-10-05 Abbott Gmbh & Co Kg Anticuerpos monoclonales contra la proteina rgm a para utilizarse en el tratamiento de degeneracion de capa de fibra de nervio retinal.
WO2011083147A1 (fr) 2010-01-08 2011-07-14 Cemm-Forschungsinstitut Für Molekulare Medizin Gmbh Inhibition de wave1 dans l'intervention médicale de maladies inflammatoires et/ou d'infections causées par un pathogène
WO2011131626A1 (fr) 2010-04-19 2011-10-27 Medizinische Universität Innsbruck Tmem195 code pour l'activité alkylglycérol monooxygénase dépendante de la tétrahydrobioptérine
SG10201600144YA (en) 2011-12-05 2016-02-26 Med & Biological Lab Co Ltd Method for detecting protein-protein interaction
EP3369746A1 (fr) 2012-01-27 2018-09-05 AbbVie Deutschland GmbH & Co KG Composition et procédé pour le diagnostic et le traitement de maladies associées à la dégénérescence des neurites
CA2866097C (fr) * 2012-03-06 2021-02-02 Vib Vzw Proteine de fusion transmembranaire-kinase et les utilisations de celle-ci
CA2951630A1 (fr) 2014-06-10 2015-12-17 Medical & Biological Laboratories Co., Ltd. Methode de determination d'une interaction proteine-proteine
EP3172563B1 (fr) 2014-07-23 2019-06-12 Institut National de la Sante et de la Recherche Medicale (INSERM) Procédés pour détecter des interactions dans des cellules eucaryotes au moyen de structures de microtubules et dynamiques
KR101823990B1 (ko) * 2016-09-07 2018-01-31 포항공과대학교 산학협력단 살아있는 세포에서 막 단백질 간의 상호작용을 정량적으로 측정하는 방법

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996023898A1 (fr) * 1995-01-31 1996-08-08 Novo Nordisk A/S Procede de detection de substances biologiquement actives
WO1998002571A1 (fr) * 1996-07-16 1998-01-22 The Regents Of The University Of California Dosages de proteines kinases faisant appel a des substrats proteiques fluorescents
WO1998034120A1 (fr) * 1997-01-31 1998-08-06 Universite De Montreal Analyses par complementation de fragments proteiques pour detecter des interactions biomoleculaires

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5804604A (en) * 1989-12-21 1998-09-08 Biogen, Inc. Tat-derived transport polypeptides and fusion proteins
US5427908A (en) * 1990-05-01 1995-06-27 Affymax Technologies N.V. Recombinant library screening methods
DE69230142T2 (de) * 1991-05-15 2000-03-09 Cambridge Antibody Technology Ltd. Verfahren zur herstellung von spezifischen bindungspaargliedern
DE69333115D1 (de) * 1992-09-22 2003-08-28 Biofocus Discovery Ltd Rekombinante viren, die an ihrer äusseren oberfläche ein nichtvirales polypeptid präsentieren

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996023898A1 (fr) * 1995-01-31 1996-08-08 Novo Nordisk A/S Procede de detection de substances biologiquement actives
WO1998002571A1 (fr) * 1996-07-16 1998-01-22 The Regents Of The University Of California Dosages de proteines kinases faisant appel a des substrats proteiques fluorescents
WO1998034120A1 (fr) * 1997-01-31 1998-08-06 Universite De Montreal Analyses par complementation de fragments proteiques pour detecter des interactions biomoleculaires

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BRODER Y C ET AL: "The ras recruitment system, a novel approach to the study of protein-protein interactions", CURRENT BIOLOGY, CURRENT SCIENCE,, GB, vol. 8, no. 20, 8 October 1998 (1998-10-08), pages 1121 - 1124, XP002135902, ISSN: 0960-9822 *
MAROUN M ET AL: "A NOVEL IN VIVO ASSAY FOR THE ANALYSIS OF PROTEIN-PROTEIN INTERACTION", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 27, no. 13, 1999, pages COMPLETE, XP000904543, ISSN: 0305-1048 *
SAKAI ET AL: "Translocation of protein kinase C-gamma and epsilon - Direct visualization in living cells using fusion protein with green fluorescent protein", THE JOURNAL OF CELL BIOLOGY, ROCKEFELLER UNIVERSITY PRESS, US, 1997, XP002078902, ISSN: 0021-9525 *
See also references of WO0017221A1 *

Also Published As

Publication number Publication date
JP2002526756A (ja) 2002-08-20
EP1115734A1 (fr) 2001-07-18
CA2345392A1 (fr) 2000-03-30
WO2000017221A9 (fr) 2000-06-29
WO2000017221A1 (fr) 2000-03-30
AU5578999A (en) 2000-04-10

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