EP1137765A4 - Appareil pour l'ecoulement tangentiel, la concentration et la fusion des cellules - Google Patents

Appareil pour l'ecoulement tangentiel, la concentration et la fusion des cellules

Info

Publication number
EP1137765A4
EP1137765A4 EP00919388A EP00919388A EP1137765A4 EP 1137765 A4 EP1137765 A4 EP 1137765A4 EP 00919388 A EP00919388 A EP 00919388A EP 00919388 A EP00919388 A EP 00919388A EP 1137765 A4 EP1137765 A4 EP 1137765A4
Authority
EP
European Patent Office
Prior art keywords
cell concentration
tangential flow
fusion apparatus
fusion
tangential
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP00919388A
Other languages
German (de)
English (en)
Other versions
EP1137765A1 (fr
Inventor
Pulse Sciences Inc Cyto
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cyto Pulse Sciences Inc
Original Assignee
Cyto Pulse Sciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cyto Pulse Sciences Inc filed Critical Cyto Pulse Sciences Inc
Publication of EP1137765A1 publication Critical patent/EP1137765A1/fr
Publication of EP1137765A4 publication Critical patent/EP1137765A4/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14—Ultrafiltration; Microfiltration
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14—Ultrafiltration; Microfiltration
    • B01D61/18—Apparatus therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14—Ultrafiltration; Microfiltration
    • B01D61/20—Accessories; Auxiliary operations
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/02—Separators
    • B03C5/022—Non-uniform field separators
    • B03C5/026—Non-uniform field separators using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion

Landscapes

  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Water Supply & Treatment (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Physics & Mathematics (AREA)
  • Electromagnetism (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Microelectronics & Electronic Packaging (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
EP00919388A 1999-03-22 2000-03-21 Appareil pour l'ecoulement tangentiel, la concentration et la fusion des cellules Withdrawn EP1137765A4 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US12558399P 1999-03-22 1999-03-22
US125583P 1999-03-22
PCT/US2000/006420 WO2000060065A1 (fr) 1999-03-22 2000-03-21 Appareil pour l'ecoulement tangentiel, la concentration et la fusion des cellules

Publications (2)

Publication Number Publication Date
EP1137765A1 EP1137765A1 (fr) 2001-10-04
EP1137765A4 true EP1137765A4 (fr) 2002-09-18

Family

ID=22420420

Family Applications (1)

Application Number Title Priority Date Filing Date
EP00919388A Withdrawn EP1137765A4 (fr) 1999-03-22 2000-03-21 Appareil pour l'ecoulement tangentiel, la concentration et la fusion des cellules

Country Status (3)

Country Link
EP (1) EP1137765A4 (fr)
CA (1) CA2374649A1 (fr)
WO (1) WO2000060065A1 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4567337B2 (ja) * 2001-12-06 2010-10-20 バイオ−ラッド ラボラトリーズ，インコーポレイティド メンブレン式融合を促進する電気穿孔キュベット用インサート
JP2006521780A (ja) * 2003-03-17 2006-09-21 バージニア テック インテレクチュアル プロパティーズ インコーポレーテッド 二相ＳＲＭ（ＴＰＳＲＭ）の固定子バック材（ｂａｃｋｍａｔｅｒｉａｌ）における磁束変化を防止する装置および方法
AU2003902363A0 (en) * 2003-05-15 2003-05-29 Life Therapeutics Limited Cell separation
EP2270130B1 (fr) * 2005-06-13 2020-04-29 Tosoh Corporation Dispositif pour la fusion des cellules et procédé d'utilisation de celui-ci
CN114574355B (zh) * 2020-11-30 2026-03-17 中国科学院大连化学物理研究所 一种外泌体纯化的装置及应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0208637A1 (fr) * 1985-06-19 1987-01-14 Centre National De La Recherche Scientifique (Cnrs) Procédé perfectionné de fusion cellulaire
US4832814A (en) * 1987-12-28 1989-05-23 E. I. Du Pont De Nemours And Company Electrofusion cell and method of making the same

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6010613A (en) * 1995-12-08 2000-01-04 Cyto Pulse Sciences, Inc. Method of treating materials with pulsed electrical fields

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0208637A1 (fr) * 1985-06-19 1987-01-14 Centre National De La Recherche Scientifique (Cnrs) Procédé perfectionné de fusion cellulaire
US4832814A (en) * 1987-12-28 1989-05-23 E. I. Du Pont De Nemours And Company Electrofusion cell and method of making the same

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of WO0060065A1 *

Also Published As

Publication number Publication date
WO2000060065A1 (fr) 2000-10-12
CA2374649A1 (fr) 2000-10-12
EP1137765A1 (fr) 2001-10-04

Similar Documents

Publication Publication Date Title
AU3227400A (en) Flow cell, analyte measurement apparatus and methods related thereto
SG105513A1 (en) Low defect density, ideal oxygen precipitating silicon
IL144654A0 (en) Pancreatic progenitor cells, methods and uses related thereto
GB2344526B (en) Syringe with filter,and filter therefor
PL346696A1 (en) Porous polymer particles
GB9800325D0 (en) Improvements in catalytic materials
EP1224970A4 (fr) Catalyseur de desulfuration profonde, son procede de preparation et procede de desulfuration l'utilisant
IL181413A0 (en) Cell regeneration
EP1308437A4 (fr) Acylacetonitriles, leur procede de preparation et acaricides renfermant ces acylacetonitriles
EP1213150A4 (fr) Procede et dispositif de detection de matiere et imprimante associee
AU3244100A (en) Chromatography devices, porous medium modules used in chromatography devices, and methods for making porous medium modules
HUP9901035A3 (en) Parapoxviruses containing foreign dna, their production and their use in vaccines
HUP0104825A3 (en) Genes and proteins, and their use
ZA200005739B (en) Fluid energy milling process and apparatus.
AU2001288106A1 (en) Diamond electrode for measuring glucose concentration, and measuring method and apparatus employing the same
PL350825A1 (en) Liquid purifying cartridge and apparatus
EP1137765A4 (fr) Appareil pour l'ecoulement tangentiel, la concentration et la fusion des cellules
GB0007933D0 (en) Assays,methods and means
ZA200001906B (en) Method and apparatus for purchasing energy.
GB9808460D0 (en) Porous container,and apparatus for use with the porous container
AU1197001A (en) B cell clonal toxins and methods for using the same
IL154544A0 (en) T CELL RECEPTOR Vbeta-Dbeta-Jbeta SEQUENCE AND METHODS FOR ITS DETECTION
GB2335410B (en) Improvements in pouches
GB2348701B (en) Assays,methods and means
GB0005693D0 (en) Auction and reverse auction system,auction and reverse auction apparatus,connecting apparatus,and auction and reverse auction method

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20010716

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: CYTO PULSE SCIENCES, INC.

RIN1 Information on inventor provided before grant (corrected)

Inventor name: CYTO PULSE SCIENCES, INC.

A4 Supplementary search report drawn up and despatched

Effective date: 20020802

AK Designated contracting states

Kind code of ref document: A4

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

RIC1 Information provided on ipc code assigned before grant

Free format text: 7C 12N 13/00 A, 7C 12M 1/00 B, 7B 01D 57/02 B, 7C 02F 1/40 B, 7C 12M 3/00 B

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION HAS BEEN WITHDRAWN

18W Application withdrawn

Effective date: 20050324