EP1179174A2 - Dispositif et procede de surveillance et de controle de fluides a activite biologique - Google Patents

Dispositif et procede de surveillance et de controle de fluides a activite biologique

Info

Publication number
EP1179174A2
EP1179174A2 EP00936736A EP00936736A EP1179174A2 EP 1179174 A2 EP1179174 A2 EP 1179174A2 EP 00936736 A EP00936736 A EP 00936736A EP 00936736 A EP00936736 A EP 00936736A EP 1179174 A2 EP1179174 A2 EP 1179174A2
Authority
EP
European Patent Office
Prior art keywords
line section
line
fluid
data acquisition
metabolite
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP00936736A
Other languages
German (de)
English (en)
Inventor
Thomas HÖFLER
Peter Holzhauer
Eckehard Walitza
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fraunhofer Gesellschaft zur Foerderung der Angewandten Forschung eV
Original Assignee
Fraunhofer Gesellschaft zur Foerderung der Angewandten Forschung eV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fraunhofer Gesellschaft zur Foerderung der Angewandten Forschung eV filed Critical Fraunhofer Gesellschaft zur Foerderung der Angewandten Forschung eV
Publication of EP1179174A2 publication Critical patent/EP1179174A2/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2304/00—Chemical means of detecting microorganisms
    • C12Q2304/40—Detection of gases
    • C12Q2304/44—Oxygen

Definitions

  • Online measurement methods usually only track the biological activity of microorganisms. This is done, for example, by measuring the oxygen or carbon dioxide content, the pH value or the concentration of fluorescence-active metabolites. However, these methods do not establish a direct correlation, for example based on oxygen consumption, between metabolic activity and the number of microorganisms present.
  • US Pat. No. 5,224,051 describes a device which, among other things, measures the content of dissolved oxygen in cooling lubricants in order to obtain information on microorganisms. However, the device does not measure the oxygen consumption in a closed volume and consequently does not establish a correlation between temperature-dependent oxygen consumption and the number of bacteria in the solution.
  • DE 44 15 444 uses the continuous determination of the oxygen transfer rate to determine the physiological conditions in microbiological cultures.
  • the oxygen transfer rate is measured from the drop in the oxygen partial pressure in the gas space with a sterilizable oxygen electrode.
  • the direct calculation of the bacterial counts from the oxygen consumption and the temperature is not disclosed.
  • the line or the line section assigned to the container represents a closed system for the fluid located and measured therein, that is to say a system which does not permit direct exchange of metabolites measured in the measuring range with the -Undefined- bypass system, the line or the line section is supplied permanently or periodically with fluid to be measured from the container assigned to it.
  • the fluid from the container is therefore generally not removed from the container, for example by means of a ladle, and fed to a closed measuring system.
  • the line is preferably in flow connection with the container, that is to say at least one of the openings of the line opens into the container, and can absorb fluid from there.
  • the fluid in the line is in a closed or quasi-closed system due to the dwell time in the line section.
  • the line section thus provides a closed system which enables an unadulterated metabolite change measurement due to the dwell time generated in the line section.
  • a closed system is made available inside a container or outside a container, the metabolite change in the fluid being able to be measured directly.
  • the invention thus relates to a method for the continuous determination of the concentration of organisms in a fluid located in a line section, the line section being assigned to a container containing the fluid to be measured and wherein in the closed system caused by the residence time of the fluid in the line section, the time-dependent Change of at least one metabolite parameter is measured by means of at least one data acquisition device.
  • the medium to be examined is removed unchanged from the process to be examined via a discharge.
  • a time delay before arrival at the measuring sensor or at the measuring probe can be generated after the discharge.
  • the time delay can be varied, in particular by the length and / or diameter of a pipeline, a capillary or the like and the flow rate of the fluid.
  • gassing of the fluid to be examined with gas mixtures or gases can take place if the content of dissolved gases, in particular oxygen, is too low and aerobic germs are to be detected.
  • gases can also be supplied which allow detection of anaerobic organisms, for example in the medical / clinical field, in sewage treatment plants and in the formation of methane and acetate by carbonate reduction by means of methanogenic and acetogenic bacteria.
  • a cleaning or sterilization procedure of the line section between individual measuring processes can be provided in all of the above-mentioned embodiments.
  • the device according to the invention has a line section with in each case an inlet and an outlet opening, these two openings or one of these two openings being able to have coupling or coupling devices which enable coupling into a line of a container or into the container itself.
  • the line, or the line section, of the device is immersed directly in the fluid of a container, or the fluid is introduced into the line section, without providing a coupling directly to the container or to one of its lines.
  • the determination of the metabolite concentration can optionally also be carried out using devices for carrying out optical measurement methods, for example UV or IR absorption, or chromatographic measurement methods using thin or thick-film sensors.
  • optical measurement methods for example UV or IR absorption, or chromatographic measurement methods using thin or thick-film sensors.
  • Semiconductor-based sensors or ion-selective electrodes or ultrasonic measuring methods are used.
  • the data from the line section determined by means of the data acquisition device or its sensor can be transmitted to the data processing system, for example, via a data transfer cable.
  • the invention also provides remote data transmission from the data acquisition device to the data processing system, for example by means of corresponding glass fiber-based systems, modems, bus systems, infrared devices or radio.
  • the data processing system 50 comprises a housing 52, a microprocessor 54 and a display device 56.
  • a liquid 80 contaminated with germs is saturated with air and in the device 100 according to the invention the dependence of the oxygen consumption on the germ count (CFU / ml) and the temperature is determined.
  • the test time is 5 to 240 minutes, preferably 10 to 60 minutes. After each attempt, the solution can be gassed with air over a frit until saturated. Samples are taken from the test facility at the start and end of the test for microbiological microbial count determination, which serve as a reference.
  • the oxygen consumption is measured in the temperature range adapted to the species, for example between 10 ° C and 40 ° C.
  • FIG. 6 shows a typical course of the oxygen content in liquids contaminated with microorganisms as a function of time for Pseudomonas spec. again.
  • the detection limit of the microorganism concentration is in the case of Pseudomonas spec. for 20 ° C with a bacterial count of> 10 4 CFU / ml and from 29 ° C with a bacterial count of> 10 2 CFU / ml. According to the invention, it is therefore particularly preferred to measure the oxygen consumption in the liquid at elevated temperature in order to lower the detection limit.
  • FIG. 2a shows a device 100 according to the invention comprising a data processing system 7, a data acquisition device 3 with the sensor 30, a line section 46 of the line 4, not shown, into which a pump 5 is integrated, and a valve 2, which is designed as a 4-way valve .
  • the 4-way valve 2 also functions here as a coupling between the line section 46 of the device 100 and the sampling line 1 and the sample return line 6, that is, parts of the line 4 of the container.
  • the 4-way valve 2 is switched so that fresh liquid 80 can flow into the line section 46 via the sampling line 1 (regeneration cycle).
  • FIG. 1 shows a device 100 according to the invention comprising a data processing system 7, a data acquisition device 3 with the sensor 30, a line section 46 of the line 4, not shown, into which a pump 5 is integrated, and a valve 2, which is designed as a 4-way valve .
  • the 4-way valve 2 also functions here as a coupling between the line section 46 of the device 100 and the sampling line 1 and the sample return line 6, that is, parts
  • the 4-way valve 2 is switched in such a way that the line section 46 represents a closed system and there is no connection via the sampling line 1 and the sample return line 6 to the container 90 (not shown) (measuring cycle).
  • the line section 46 can be designed as a hose, tube or hollow fiber bundle.
  • liquid 80 with a 10-minute dwell time.
  • the following sample material from line section 46 arrives with increasing dwell time, where at 10 minutes after the switchover, there was a dwell time of 20 minutes for the liquid 80 transported back.
  • the subsequent liquid then arrives at the data acquisition device 3 with a 10-minute dwell time.
  • the liquid flow, the geometry of the line section 46 and the sensor 30 are selected so that an optimal overflow of the sensor 30 is ensured.
  • FIGS. 4a and 4b represent a further embodiment of a device 100 according to the invention and of the method carried out with it, a 6-way valve 2 being used instead of the 4-way valve 2 of FIGS. 3a and 3b.
  • the data acquisition device 3 with its sensor 30 optionally determines the metabolite parameter at the line inlet 61 (see FIG. 4a) or line outlet 63 (see FIG. 4b).
  • the line section 46 is permanently supplied with fresh liquid 80 by means of a pump 5.
  • the sampling line 1 as well as the sample return line 6 are connected to the container 90. Liquid 80 reaches the 6-way valve 2 through the sampling line 1 and the pump 5 and then initially into the inlet 61 of the line section 46.
  • the described liquid flow through the line section 46 is continuously and simultaneously subjected to a determination of the metabolite parameters at the two data acquisition devices 3 and 3 ', so that the differences in the metabolite parameters generated by the residence time of the liquid in the line section are continuously fed to the data processing system 7.
  • the liquid can then be returned to the container 90 via the sample return line 6.
  • the dwell time can be varied by the flow tube geometry and the flow speed that can be set with the pump 5.
  • the measured values ascertained are fed to the data processing system 7 via the data transfer line 40, where they are evaluated arithmetically by means of the stored program for determining the microorganism concentration and displayed in a display device as CFU / ml.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Sustainable Development (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Dispositif et procédé permettant de déterminer la concentration d'organismes dans un fluide. Selon ledit procédé, des paramètres relatifs à des métabolites sont enregistrés à l'aide d'un dispositif d'enregistrement de données, et sont ensuite convertis.
EP00936736A 1999-05-12 2000-05-12 Dispositif et procede de surveillance et de controle de fluides a activite biologique Withdrawn EP1179174A2 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE19921999 1999-05-12
DE19921999A DE19921999C2 (de) 1999-05-12 1999-05-12 Vorrichtung und Verfahren zur Überwachung und Kontrolle von biologisch aktiven Fluiden
PCT/EP2000/004289 WO2000070078A2 (fr) 1999-05-12 2000-05-12 Dispositif et procede de surveillance et de controle de fluides a activite biologique

Publications (1)

Publication Number Publication Date
EP1179174A2 true EP1179174A2 (fr) 2002-02-13

Family

ID=7907903

Family Applications (1)

Application Number Title Priority Date Filing Date
EP00936736A Withdrawn EP1179174A2 (fr) 1999-05-12 2000-05-12 Dispositif et procede de surveillance et de controle de fluides a activite biologique

Country Status (6)

Country Link
US (1) US6677132B1 (fr)
EP (1) EP1179174A2 (fr)
JP (1) JP2002543849A (fr)
CA (1) CA2372851A1 (fr)
DE (1) DE19921999C2 (fr)
WO (1) WO2000070078A2 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4448673B2 (ja) * 2003-08-22 2010-04-14 シスメックス株式会社 細菌分析装置および方法
ITBO20070163A1 (it) 2007-03-12 2008-09-13 Ali Spa Macchina e metodo per la produzione e l'erogazione di prodotti di consumo alimentari liquidi o semiliquidi.
CA2726868A1 (fr) * 2008-06-13 2009-12-17 Foss Analytical A/S Procede de controle de procedes biotechnologiques
JP7209701B2 (ja) * 2017-10-03 2023-01-20 アベイルズ メディカル，インコーポレイテッド レドックス反応に基づいて微生物の濃度及び抗感染剤に対する微生物の感受性を決定する装置、システム、及び方法
DE102018102658A1 (de) 2018-02-06 2019-08-08 AdvaTec Projects GmbH & Co. KG Messgerät und Verfahren zur Bestimmung des Verkeimungsgrades von Prozessflüssigkeiten
CN109061089A (zh) * 2018-08-30 2018-12-21 郑州鼎为实业有限公司 一种用于水污染检测的实验台
CN109486969A (zh) * 2018-10-26 2019-03-19 贵州茅台酒股份有限公司 一种定向筛选产生正丙醇菌株的方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3731522A (en) 1970-09-30 1973-05-08 Robertshaw Controls Co Method and apparatus for determining oxygen consumption rate in sewage
US4220715A (en) 1978-08-03 1980-09-02 Johnston Laboratories, Inc. Apparatus for and method of detection of significant bacteriuria in urine samples through measurement of head space gas oxygen consumption in a closed-vial system
US5224051A (en) 1989-05-19 1993-06-29 Cincinnati Milacron, Inc. Fluid condition monitoring and controlling system for a metalworking fluid central system
US5614378A (en) * 1990-06-28 1997-03-25 The Regents Of The University Of Michigan Photobioreactors and closed ecological life support systems and artifificial lungs containing the same
US5702951A (en) 1990-07-04 1997-12-30 Commonwealth Scientific And Industrial Research Organisation Continuous RBCOD measurement
US5422014A (en) * 1993-03-18 1995-06-06 Allen; Ross R. Automatic chemical monitor and control system
DE4415444C2 (de) * 1993-05-12 2002-10-31 Jochen Buechs Automatisches Meßsystem zur sterilen on-line Bestimmung der Sauerstofftransferrate (OTR) in Schüttelkolben
DE4429809A1 (de) 1994-08-23 1996-02-29 Ziemann Gmbh A Verfahren zur vollautomatischen Gärprozeßregelung bei der Herstellung alkoholischer Getränke nach dem "Umpumpverfahren"
DE19605753C2 (de) * 1996-02-16 1998-05-14 Norbert Pautz Verfahren und Vorrichtung zur Detektion von stoffwechselaktiven, unlädierten, ungestressten Mikroorganismen - quantitativ und qualitativ im "Sub-ppb-Bereich" innerhalb von Minuten

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO0070078A2 *

Also Published As

Publication number Publication date
WO2000070078A2 (fr) 2000-11-23
US6677132B1 (en) 2004-01-13
DE19921999C2 (de) 2003-02-13
CA2372851A1 (fr) 2000-11-23
JP2002543849A (ja) 2002-12-24
WO2000070078A3 (fr) 2001-03-01
DE19921999A1 (de) 2000-11-16

Similar Documents

Publication Publication Date Title
EP0425587B1 (fr) Procede pour la mise en evidence d'activites biologiques dans un echantillon et dispositif pour la mise en oeuvre de ce procede
DE69515554T2 (de) Testverfahren für Enzyme und Vorrichtung dafür
DE69816663T2 (de) Vorrichtung zur bestimmung von analyten in lösungen
DE69431481T2 (de) Überwachung der biologischen aktivität in abwasser
DE69420962T2 (de) Vorrichtung zur analyse einer flüssigkeit
AT505306B1 (de) Vorrichtung zur überwachung von wasser auf mikrobielle keime
DE9421731U1 (de) Vorrichtung zum Analysieren eines Fluidmediums
WO2018185052A1 (fr) Procédé pour la régulation d'un procédé biotechnologique
DE69702423T2 (de) Methode und Gerät zur Bestimmung von Substanzen in Lösungen, Suspensionen und Emulsionen durch differentielle pH-Messung
DE19921999C2 (de) Vorrichtung und Verfahren zur Überwachung und Kontrolle von biologisch aktiven Fluiden
EP0821231B1 (fr) Appareil pour l'analyse de liquides
DE69838673T2 (de) Mikrobiosensor zum kontinuierlichen nachweis von chemischen substanzen in flüssigkeiten
DE69929686T2 (de) Verfahren zum messen der nitrifikationsraten in biochemischen prozessen
WO2001090718A1 (fr) Procede et dispositif pour determiner la concentration d'analytes
EP0995098A1 (fr) Procede et dispositif pour prelever en serie des echantillons
EP0476595A2 (fr) Système pour l'analyse du type à injection dans un écoulement
DE3927856A1 (de) Verfahren zur prozessfuehrung mindestens eines bioreaktors fuer pflanzliche zellkulturen
DE19605753C2 (de) Verfahren und Vorrichtung zur Detektion von stoffwechselaktiven, unlädierten, ungestressten Mikroorganismen - quantitativ und qualitativ im "Sub-ppb-Bereich" innerhalb von Minuten
EP0980515A1 (fr) Dispositif de detection de biomolecules et de substances dissoutes dans des liquides
DD142823A3 (de) Verfahren und vorrichtung zur automatischen analyse der umweltbelastung von luft,trink-,brauch-und abwasser
DE10134656B4 (de) Mobile Analytik zur VFA Bestimmung
DE2655265C3 (de) Verfahren zur automatischen Vorbereitung von aus Zellsuspensionen bestehenden Proben zum Zwecke der nachfolgenden Bestimmung intra- und/oder extrazellulärer Stoffe durch eine kontinuierlich aufeinanderfolgende Behandlung einer Mehrzahl von Proben
DE102007027914A1 (de) Sterilisierbarer Sensor zur Überwachung von biochemischen Prozessen in Fermentern
DE19917955A1 (de) Verfahren und Vorrichtung zur Überwachung aerober umweltbiotechnologischer Prozesse
DE202006008928U1 (de) Miniaturchemostat

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20011212

AK Designated contracting states

Kind code of ref document: A2

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

GRAH Despatch of communication of intention to grant a patent

Free format text: ORIGINAL CODE: EPIDOS IGRA

RTI1 Title (correction)

Free format text: DEVICE AND METHOD FOR DETERMINING THE CONCENTRATION OF MICROORGANISMS

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20021224