EP1194565A1 - Genes de corynebacterium glutamicum pour la biosynthese de l'acide folique et leur utilisation pour la production microbienne d'acide folique - Google Patents

Genes de corynebacterium glutamicum pour la biosynthese de l'acide folique et leur utilisation pour la production microbienne d'acide folique

Info

Publication number
EP1194565A1
EP1194565A1 EP00945815A EP00945815A EP1194565A1 EP 1194565 A1 EP1194565 A1 EP 1194565A1 EP 00945815 A EP00945815 A EP 00945815A EP 00945815 A EP00945815 A EP 00945815A EP 1194565 A1 EP1194565 A1 EP 1194565A1
Authority
EP
European Patent Office
Prior art keywords
polypeptide
folic acid
seq
amino acids
deletion
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP00945815A
Other languages
German (de)
English (en)
Inventor
Matthias Mack
Karin Herbster
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sygnis Pharma AG
Original Assignee
Axaron Bioscience AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Axaron Bioscience AG filed Critical Axaron Bioscience AG
Publication of EP1194565A1 publication Critical patent/EP1194565A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1235—Diphosphotransferases (2.7.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
    • C12P17/182—Heterocyclic compounds containing nitrogen atoms as the only ring heteroatoms in the condensed system

Definitions

  • the present invention relates to the production process for folic acid by fermentation using a genetically modified organism.
  • This invention consists of the nucleotide sequences of four genes (folE, folP, folB and folK) from Corynebacterium glutamicum for folic acid biosynthesis and their use for the microbial production of folic acid. These four genes form an operon and are transcribed in the following order: folE, folP, folB, folK.
  • Folic acid is essential for animal organisms. Its derivative tetrahydrofolate is a very versatile carrier of activated single-carbon units in cells of the animal organism. Folic acid consists of three groups: a substituted pteridine ring, p-aminobenzoate and glutamate. Mammals cannot synthesize a pteridine ring. They take in folic acid from food and from microorganisms in their intestinal tract. Folic acid deficiency mainly leads to lesions in the mucous membranes.
  • Folic acid is mainly used as a food additive.
  • Microorganisms can be used for the fermentative production of folic acid. They can be optimized in their folic acid biosynthesis performance by genetically modifying the biosynthetic pathway of folic acid.
  • genetic engineering means increasing the number of copies and / or the rate of transcription of the genes of the biosynthetic pathway for folic acid.
  • the proportion of gene product and thus also the intracellular enzyme activity increases.
  • Increased enzyme activity leads to an increased rate of conversion of food (eg glucose) to folic acid and thus to an increased product concentration.
  • the nucleotide sequences of the genes of the folic acid biosynthetic pathway must be identified.
  • This invention is concerned with four new gene sequences for the folic acid biosynthesis from Corynebacterium glutamicum and with their use for the microbial production of folic acid.
  • Part of the invention is the folE gene product.
  • SEQ ID NO. 2 describes a polypeptide sequence.
  • the folE gene product encodes a polypeptide of 202 amino acids with a molecular weight of 22029 Da.
  • the present invention is also concerned with functional derivatives of this polypeptide, which can be obtained if one in SEQ ID NO. 2 one or more amino acids, preferably up to 25% of the amino acids, preferably up to 15% of the amino acids, are replaced by deletion, insertion or substitution or by a combination of deletion, insertion and substitution.
  • the term functional derivative means that the enzymatic activity of the derivative is still in the same order of magnitude as that of the polypeptide with the sequence SEQ ID NO. 2.
  • Another part of the invention is the folP gene product.
  • SEQ ID NO. 4 describes a polypeptide sequence.
  • the folP gene product encodes a polypeptide of 285 amino acids with a molecular weight of 29520 Da.
  • the present invention is also concerned with functional derivatives of this polypeptide, which can be obtained if one in SEQ ID NO. 4 one or more amino acids, preferably up to 40% of the amino acids, preferably up to 25% of the amino acids, are replaced by deletion, insertion or substitution or by a combination of deletion, insertion and substitution.
  • the term functional derivative means that the enzymatic activity of the derivative is still of the same order of magnitude as that of the polypeptide with the sequence SEQ ID NO. 4th
  • SEQ ID NO. 6 describes a polypeptide sequence.
  • the folB gene product encodes a polypeptide of 131 amino acids with a molecular weight of 14020 Da.
  • the present invention is also concerned with functional derivatives of this polypeptide, which can be obtained if one in SEQ ID NO. 6 one or more amino acids, preferably up to 30% of the amino acids, preferably up to 20% of the amino acids, are replaced by deletion, insertion or substitution or by a combination of deletion, insertion and substitution.
  • the term functional derivative means that the enzymatic activity of the derivative is still of the same order of magnitude as that of the polypeptide with the sequence SEQ ID R. 6.
  • SEQ ID NO. 8 describes a polypeptide sequence.
  • the folK gene product encodes a polypeptide of 160 amino acids with a molecular weight of 18043 Da.
  • the present invention is also concerned with functional derivatives of this polypeptide, which can be obtained if one in SEQ ID NO. 8 by deletion, insertion or substitution or by a combination of deletion, Insertion and substitution of one or more amino acids, preferably replacing up to 40% of the amino acids, preferably up to 30% of the amino acids.
  • functional derivative it is meant that the enzymatic activity of the derivative is still in the same order of magnitude as that of the polypeptide with the sequence SEQ ID NO. 8th.
  • polynucleotide sequences which encode the polypeptides described above.
  • the polynucleotide sequences can be generated starting from sequences which are isolated from Corynebacterium glutamicum (ie SEQ ID NO. 1, 3, 5 and 7) by modifying these sequences by site-directed mutagenesis or after back-translating the corresponding polypeptide with genetic code carries out a total chemical synthesis.
  • polynucleotide sequences can preferably be used for the transformation of host organisms, and preferably of microorganisms, in the form of gene constructs which contain at least one copy of one of these polynucleotides together with at least one regulatory sequence.
  • Regulatory sequences include promoters, terminators, enhancers and ribosomal binding sites.
  • Preferred host organisms for the transformation with these gene constructs are Coryneibacterium and Bacillus species. Any eukaryotic microorganism can also be used, preferably yeast strains of the genus Ashbya, Candlda, Plchla, Saccharomyces and Hansenula.
  • Another part of the invention consists in the process for the preparation of folic acid by culturing a host organism which is transformed in the manner described above and in the subsequent isolation of the folic acid.
  • the trained personnel are familiar with the processes and procedures for cultivating microorganisms and isolating folic acid from a microbial production.
  • DNA from the genome of Corynebacterium glutamlcum ATCC 13032 can be obtained by standard methods which have already been described, e.g. B. by J. Altenbuchner and J. Cullum (1984, Mol. Gen. Genet. 195: 134-138).
  • the genome library can be prepared according to standard regulations (e.g. Sambrook, J. et al. (1989) Molecular cloning: a laboratory manual, Cold Spring Harbor Laboratory Press) with any cloning vector, e.g. pBluescript II KS- (Stratagene) or ZAP Express TM (Stratagene). Any fragment size can be used, preferably 5'au3AI fragments with a length of 2-9 kb, which can be integrated into cloning vectors with digested BamHI.
  • E. coli clones can be selected from the genome library shown in Example 1.
  • E. coli cells are cultivated according to standard ancestors in suitable media (e.g. LB supplemented with 100 mg / 1 ampicillin), after which the plasmid DNA can be isolated. If one clones genome fragments from the DNA of Corynebacterium glutamlcum in pBluescript II KS- (see Example 1), the DNA can be sequenced with the help of the oligonucleotides 5 '-AATTAACCCTCACTAAAGGG-3' and 5'-GTAATACGACTCACTATAGGGC-3 '.
  • nucleotide sequences can e.g. using the BLASTX algorithm (Altschul et al. (1990) J. Mol. Biol. 215: 403-410). In this way, one can discover new sequences and elucidate the function of these new genes.
  • Example 3 The analysis of the E. coli clones, as described in Example 2, which was followed by the analysis of the sequences obtained in Example 3, resulted in a sequence as described with SEQ ID NO. 3 is described.
  • this sequence showed similarity to dihydropteroate synthases (FolP; EC 2.5.1.15) from different organisms. The greatest similarity was with the dihydropteroate synthase (FolP) from Mycobacterlum tuberculosls (NRDB 006274; 53% agreement at the amino acid level).
  • Example 3 When the E. coli clones were analyzed as described in Example 2, followed by the analysis of the sequences obtained in Example 3, a sequence was obtained as described with SEQ ID NO. 5 is described.
  • this sequence showed similarity to dihydroneopterin aldolases (FolB; EC 4.1.2.25) from different organisms. The greatest similarity 5 was with the dihydroneopterin aldolase (FolB) from Mycobacterlum tuberculosis (NRDB 006275; 61% agreement at the amino acid level).
  • Example 5 The analysis of the E. coli clones as described in Example 2, which was followed by the analysis of the sequences obtained in Example 3, resulted in one Sequence as shown with SEQ ID NO. 7 is described.
  • this sequence showed similarity with 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinases (FolK; EC 2.7.6.3) from different organisms. The greatest similarity was with that
  • GTP cyclohydrolase I for dihydropteroate synthase, for dihydroneopterin aldolase and for 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase from Corynebacterium glutamicum for the production of folic acid
  • the genes for the GTP cyclohydrolase I, for the dihydropteroate synthase, for the dihydroneopterin aldolase and for the 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase from Corynebacterium glutamicum can be obtained with the aid of suitable cloning and expression systems introduce into Corynebacterium glutamicum or into any other microorganism. Genetically modified microorganisms can be produced which differ from the wild-type organism with regard to the activity or the number of gene copies. These new, genetically modified strains can be used to produce folic acid.
  • SEQ ID NO. 1 DNA (folE)
  • SEQ ID NO. 2 amino acid (FolE)
  • SEQ ID NO. 3 DNA (folP)
  • SEQ ID NO. 4 amino acid (FolP)
  • SEQ ID NO. 6 amino acid (FolB)
  • SEQ ID NO. 7 DNA (folK)
  • SEQ ID NO. 8 amino acid (FolK)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne des séquences nucléotidiques de quatre gènes (folE, folP, folB et folK) de Corynebacterium glutamicum servant à la biosynthèse de l'acide folique et leur utilisation pour la production microbienne d'acide folique.
EP00945815A 1999-06-25 2000-06-23 Genes de corynebacterium glutamicum pour la biosynthese de l'acide folique et leur utilisation pour la production microbienne d'acide folique Withdrawn EP1194565A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE19929363 1999-06-25
DE19929363A DE19929363A1 (de) 1999-06-25 1999-06-25 Gene aus Corynebacterium glutamicum für die Folsäurebiosynthese und ihr Einsatz zur mikrobiellen Herstellung von Folsäure
PCT/EP2000/005864 WO2001000845A1 (fr) 1999-06-25 2000-06-23 Genes de corynebacterium glutamicum pour la biosynthese de l'acide folique et leur utilisation pour la production microbienne d'acide folique

Publications (1)

Publication Number Publication Date
EP1194565A1 true EP1194565A1 (fr) 2002-04-10

Family

ID=7912677

Family Applications (1)

Application Number Title Priority Date Filing Date
EP00945815A Withdrawn EP1194565A1 (fr) 1999-06-25 2000-06-23 Genes de corynebacterium glutamicum pour la biosynthese de l'acide folique et leur utilisation pour la production microbienne d'acide folique

Country Status (8)

Country Link
EP (1) EP1194565A1 (fr)
KR (1) KR20020026469A (fr)
CN (1) CN1371418A (fr)
AU (1) AU5978200A (fr)
CA (1) CA2377458A1 (fr)
DE (1) DE19929363A1 (fr)
WO (1) WO2001000845A1 (fr)
ZA (1) ZA200200582B (fr)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1298854C (zh) 1999-07-02 2007-02-07 味之素株式会社 编码蔗糖pts酶ⅱ的dna
US6958228B2 (en) 2000-08-02 2005-10-25 Degussa Ag Nucleotide sequence which code for the metH gene
US6942996B2 (en) 2000-08-02 2005-09-13 Degussa Ag Isolated polynucleotide from Corynebacterium encoding a homocysteine methyltransferase
DE10039049A1 (de) 2000-08-10 2002-02-21 Degussa Neue für das IysR3-Gen kodierende Nukleotidsequenzen
DE10039044A1 (de) 2000-08-10 2002-02-21 Degussa Neue für das IysR1-Gen kodierende Nukleotidsequenzen
DE10039043A1 (de) 2000-08-10 2002-02-21 Degussa Neue für das luxR-Gen kodierende Nukleotidsequenzen
AU2001291658A1 (en) 2000-08-26 2002-03-13 Degussa A.G. Nucleotide sequences which code for the ccpa2 gene
US6812016B2 (en) 2000-09-02 2004-11-02 Degussa Ag Nucleotide sequences which code for the metY gene
US6815196B2 (en) 2000-09-02 2004-11-09 Degussa Ag Nucleotide sequences encoding o-succinylhomoserine sulfhydrylase
WO2002020792A1 (fr) 2000-09-09 2002-03-14 Degussa Ag Sequences nucleotidiques codant le gene dep33
US6759224B2 (en) 2000-09-09 2004-07-06 Degussa Ag Nucleotide sequences which code for the sahH gene
DE10045496A1 (de) 2000-09-14 2002-03-28 Degussa Neue für das ptsi-Gen kodierende Nukleotidsequenzen
DE10055870A1 (de) 2000-11-10 2002-05-29 Degussa Neue für das nadC-Gen kodierende Nukleotidsequenzen
DE10055869A1 (de) 2000-11-10 2002-05-29 Degussa Neue für das nadA-Gen kodierende Nukleotidsequenzen
EP1262541A1 (fr) * 2001-05-28 2002-12-04 Stichting Top-Instituut Voedselwetenschappen Production de l'acide folique biodisponible
US7468262B2 (en) 2003-05-16 2008-12-23 Ajinomoto Co., Inc. Polynucleotides encoding useful polypeptides in corynebacterium glutamicum ssp. lactofermentum
CN1952114B (zh) * 2005-10-20 2010-04-14 浙江爱迪亚营养科技开发有限公司 一种谷氨酸棒杆菌及其应用于制备烟酰胺的方法
CN109810991B (zh) * 2019-03-02 2021-11-12 昆明理工大学 二氢蝶酸合酶基因folP的用途
CN111235169A (zh) * 2020-02-03 2020-06-05 昆明理工大学 一种GTP环化水解酶I基因folE及应用
CN112852844A (zh) * 2021-03-05 2021-05-28 昆明理工大学 羟甲基二氢蝶呤焦磷酸激酶基因folK的用途

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5968788A (en) * 1995-08-28 1999-10-19 Toray Industries, Inc. Method for producing folic acid

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO0100845A1 *

Also Published As

Publication number Publication date
CA2377458A1 (fr) 2001-01-04
ZA200200582B (en) 2003-03-26
KR20020026469A (ko) 2002-04-10
CN1371418A (zh) 2002-09-25
DE19929363A1 (de) 2000-12-28
WO2001000845A1 (fr) 2001-01-04
AU5978200A (en) 2001-01-31

Similar Documents

Publication Publication Date Title
EP1194565A1 (fr) Genes de corynebacterium glutamicum pour la biosynthese de l'acide folique et leur utilisation pour la production microbienne d'acide folique
DE102007052463A1 (de) Fermentative Gewinnung von Aceton aus erneuerbaren Rohstoffen mittels neuen Stoffwechselweges
DE60113448T2 (de) Klonierungsvektoren und expressionsvektoren aus rhodococcus
WO2001000802A2 (fr) Sequences partielles des genes du metabolisme primaire et secondaire de corynebacterium glutamicum et leur utilisation pour la production microbienne de metabolites primaires et secondaires
EP0751995A1 (fr) Biosynthese de la riboflavine dans des champignons
EP1196602A1 (fr) Sequence de deshydrogenase de dihydroorotate a partir de corynebacterium glutamicum et son utilisation dans la production de pyrimidines et/ou de composes derives de pyrimidine
DE69327170T2 (de) Verbesserte indol-biosynthese
DE102004006526A1 (de) Neues Lysindecarboxylase-Gen und Verfahren zur Herstellung von L-Lysin
DE112019000467T5 (de) Rekombinanter Mikroorganismus, Verfahren zu dessen Herstellung und seine Anwendung bei der Herstellung von Coenzym Q10
DE69619948T2 (de) Neues Nitrilase-Gen
DE69131015T2 (de) Rekombinante zellen, die stark chromosomal-integrierte heterologe gene exprimieren
WO1998029539A2 (fr) Sequences partielles de genes de biosynthese de purine de l'ashbya gossypii et leur utilisation dans la synthese de riboflavine par des microbes
EP0722500B1 (fr) Genes permettant le metabolisme de la butyrobetaine/crotonobetaine-l-carnitine et leur utilisation pour la production microbiologique de l-carnitine
DE4238904A1 (de) Riboflavin-Synthese in Hefen
AU708538B2 (en) Sucrose metabolism mutants
DE60118401T2 (de) Gen, das für das gumd polypeptid aus methylomonas sp. kodiert und das an der herstellung von exopolysacchariden beteiligt ist
EP1244776B1 (fr) Gene de tetrahydropyrimidine dioxygenase, polypeptides codes par ces genes, et procedes de fabrication de ces polypeptides
EP2267007A1 (fr) Nouveaux produits génétiques formant ou recyclant des acides poly-aminés à partir de bacillus licheniformis et procédé de production biotechnologique amélioré basé sur ceux-ci
DE3888076T2 (de) Verfahren zur Herstellung von L-Threonin.
WO2022161569A1 (fr) Production de 3,4-dihydroxybenzoate à partir de d-xylose en utilisant des bactéries coryneformes
EP0726320B1 (fr) Préparation microbiologique de 5-cétogluconate
WO2011076370A1 (fr) Saccharose mutase à spécificité de produit améliorée
EP2499253A2 (fr) Micro-organismes présentant une activé de sucrose mutase accrue
DE10335447A1 (de) Aminooxidase
DE102021134081A1 (de) Rekombinanter mikroorganismus in welchen das glutarsäure-transporter-gen eingeführt wurde und verfahren zur herstellung von glutarsäure unter verwendung desselben

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20020119

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

AX Request for extension of the european patent

Free format text: AL;LT PAYMENT 20020119;LV PAYMENT 20020119;MK;RO PAYMENT 20020119;SI PAYMENT 20020119

RIN1 Information on inventor provided before grant (corrected)

Inventor name: HERBSTER, KARIN

Inventor name: MACK, MATTHIAS

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20010403