EP1214978A3 - Labortemperiereinrichtung zur Temperierung auf unterschiedliche Temperaturen - Google Patents

Labortemperiereinrichtung zur Temperierung auf unterschiedliche Temperaturen Download PDF

Info

Publication number
EP1214978A3
EP1214978A3 EP01125514A EP01125514A EP1214978A3 EP 1214978 A3 EP1214978 A3 EP 1214978A3 EP 01125514 A EP01125514 A EP 01125514A EP 01125514 A EP01125514 A EP 01125514A EP 1214978 A3 EP1214978 A3 EP 1214978A3
Authority
EP
European Patent Office
Prior art keywords
samples
conditioning device
thermal conditioning
temperatures
multiple temperatures
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP01125514A
Other languages
English (en)
French (fr)
Other versions
EP1214978A2 (de
EP1214978B1 (de
Inventor
Ernst Tennstedt
Lars Dr. Peters
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eppendorf SE
Original Assignee
Eppendorf SE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eppendorf SE filed Critical Eppendorf SE
Publication of EP1214978A2 publication Critical patent/EP1214978A2/de
Publication of EP1214978A3 publication Critical patent/EP1214978A3/de
Application granted granted Critical
Publication of EP1214978B1 publication Critical patent/EP1214978B1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/54—Heating or cooling apparatus; Heat insulating devices using spatial temperature gradients
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Control Of Temperature (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Feedback Control In General (AREA)
  • Control Of Heat Treatment Processes (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Devices For Use In Laboratory Experiments (AREA)

Abstract

Eine Labortemperiereinrichtung zur gemeinsamen Temperierung von Reaktionsproben in mindestens zwei Schritten in jeweils zugeordneten bestimmten Temperaturbereichen, welche als Schrittfolge wiederholt nacheinander ausgeführt werden, wobei die Labortemperiereinrichtung in einem beliebig ausgewählten ersten Schritt der Schrittfolge mehrere jeweils wenigstens eine Probe enthaltende erste Gruppen von Proben auf innerhalb der Gruppen gleiche und zwischen den Gruppen unterschiedliche Temperaturen innerhalb des dem ersten Schritt zugeordneten ersten Temperaturbereiches bringt, ist dadurch gekennzeichnet, daß in einem beliebig ausgewählten zweiten Schritt der Schrittfolge, wenn das Reaktionsprodukt bei den beiden Schritten hinsichtlich gleicher Auswertparameter beeinflußt wird, bei mindestens einer der ersten Gruppen wenigstens zwei der Proben verschiedenen zweiten Gruppen angehören, die auf innerhalb der Gruppen gleiche und zwischen den Gruppen unterschiedliche Temperaturen innerhalb des dem zweiten Schritt zugeordneten zweiten Temperaturbereiches gebracht sind, und wobei, wenn die Auswertparameter unterschiedlich sind, wenigstens zwei beliebige der Proben unterschiedlichen dritten Gruppen angehören, die auf innerhalb der Gruppen gleiche und zwischen den Gruppen unterschiedliche Temperaturen innerhalb des dem zweiten Schritt zugeordneten zweiten Temperaturbereiches gebracht sind.
EP01125514A 2000-12-12 2001-10-25 Verfahren zur Temperierung von Proben auf unterschiedliche Temperaturen in einer Labortemperiereinrichtung Expired - Lifetime EP1214978B1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10062889 2000-12-12
DE10062889A DE10062889A1 (de) 2000-12-12 2000-12-12 Labortemperiereinrichtung zur Temperierung auf unterschiedliche Temperaturen

Publications (3)

Publication Number Publication Date
EP1214978A2 EP1214978A2 (de) 2002-06-19
EP1214978A3 true EP1214978A3 (de) 2003-11-19
EP1214978B1 EP1214978B1 (de) 2006-06-21

Family

ID=7667517

Family Applications (1)

Application Number Title Priority Date Filing Date
EP01125514A Expired - Lifetime EP1214978B1 (de) 2000-12-12 2001-10-25 Verfahren zur Temperierung von Proben auf unterschiedliche Temperaturen in einer Labortemperiereinrichtung

Country Status (5)

Country Link
US (2) US20020142349A1 (de)
EP (1) EP1214978B1 (de)
JP (1) JP2002233774A (de)
AT (1) ATE330705T1 (de)
DE (2) DE10062889A1 (de)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7452712B2 (en) 2002-07-30 2008-11-18 Applied Biosystems Inc. Sample block apparatus and method of maintaining a microcard on a sample block
US20050237528A1 (en) * 2003-09-19 2005-10-27 Oldham Mark F Transparent heater for thermocycling
US7570443B2 (en) 2003-09-19 2009-08-04 Applied Biosystems, Llc Optical camera alignment
EP2608088B1 (de) * 2011-12-20 2018-12-12 F. Hoffmann-La Roche AG Verbessertes Verfahren zur Analyse von Nukleinsäure
US9188514B1 (en) * 2013-05-23 2015-11-17 The United States Of America As Represented By The Secretary Of The Navy System and method for producing a sample having a monotonic doping gradient of a diffusive constituent or interstitial atom or molecule

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0290722A2 (de) * 1987-05-15 1988-11-17 Limitek Oy Temperatur-Gradient-Inkubator
US5525300A (en) * 1993-10-20 1996-06-11 Stratagene Thermal cycler including a temperature gradient block
DE19646115A1 (de) * 1996-11-08 1998-05-14 Eppendorf Geraetebau Netheler Temperierblock mit Temperiereinrichtungen
DE29917313U1 (de) * 1999-10-01 2001-02-15 MWG-BIOTECH AG, 85560 Ebersberg Vorrichtung zur Durchführung chemischer oder biologischer Reaktionen

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1323309A (en) * 1969-12-24 1973-07-11 Toyo Kagaku Sangyo Kk Nobe A Apparatus for establishing temperature gradients in specimen containers
FI81831C (fi) * 1989-03-06 1990-12-10 Biodata Oy Temperaturgradient-inkubator foer undersoekning av temperaturavhaengiga fenomen.
US5255976A (en) * 1992-07-10 1993-10-26 Vertex Pharmaceuticals Incorporated Temperature gradient calorimeter
DE29623597U1 (de) * 1996-11-08 1999-01-07 Eppendorf - Netheler - Hinz Gmbh, 22339 Hamburg Temperierblock mit Temperiereinrichtungen
GB9720926D0 (en) * 1997-10-03 1997-12-03 Molecular Sensors Ltd Intelligent control of nucleic amplification procedures including the polymerase chain reaction
US6337435B1 (en) * 1999-07-30 2002-01-08 Bio-Rad Laboratories, Inc. Temperature control for multi-vessel reaction apparatus
WO2001062389A2 (en) * 2000-02-23 2001-08-30 Mj Research, Inc. Thermal cycler allowing two-dimension temperature gradient generation and hold time optimization
US7727479B2 (en) * 2000-09-29 2010-06-01 Applied Biosystems, Llc Device for the carrying out of chemical or biological reactions
DE10062890A1 (de) * 2000-12-12 2002-06-27 Eppendorf Ag Labortemperiereinrichtung zur Temperierung von Reaktionsproben

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0290722A2 (de) * 1987-05-15 1988-11-17 Limitek Oy Temperatur-Gradient-Inkubator
US5525300A (en) * 1993-10-20 1996-06-11 Stratagene Thermal cycler including a temperature gradient block
DE19646115A1 (de) * 1996-11-08 1998-05-14 Eppendorf Geraetebau Netheler Temperierblock mit Temperiereinrichtungen
DE29917313U1 (de) * 1999-10-01 2001-02-15 MWG-BIOTECH AG, 85560 Ebersberg Vorrichtung zur Durchführung chemischer oder biologischer Reaktionen

Also Published As

Publication number Publication date
DE10062889A1 (de) 2002-06-27
EP1214978A2 (de) 2002-06-19
JP2002233774A (ja) 2002-08-20
ATE330705T1 (de) 2006-07-15
EP1214978B1 (de) 2006-06-21
US20020142349A1 (en) 2002-10-03
US20050196873A1 (en) 2005-09-08
DE50110234D1 (de) 2006-08-03

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