EP1280888A1 - Zellkukturverfahren, zellmassen so hergestellt und ihre verwendung - Google Patents

Zellkukturverfahren, zellmassen so hergestellt und ihre verwendung

Info

Publication number
EP1280888A1
EP1280888A1 EP01927982A EP01927982A EP1280888A1 EP 1280888 A1 EP1280888 A1 EP 1280888A1 EP 01927982 A EP01927982 A EP 01927982A EP 01927982 A EP01927982 A EP 01927982A EP 1280888 A1 EP1280888 A1 EP 1280888A1
Authority
EP
European Patent Office
Prior art keywords
cells
organoids
spheroids
sheets
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP01927982A
Other languages
English (en)
French (fr)
Inventor
Alain Othon André MILLER
Nathalie Liliane Paule Ghislaine Ropson-Kenda
Christophe Alexandre Maurice Soler
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
La Region Wallonne
Original Assignee
4C Biotech SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 4C Biotech SA filed Critical 4C Biotech SA
Publication of EP1280888A1 publication Critical patent/EP1280888A1/de
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C12N5/0075—General culture methods using substrates using microcarriers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00—Drugs for disorders of the endocrine system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C12N5/0677—Three-dimensional culture, tissue culture or organ culture; Encapsulated cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • C12N5/0698—Skin equivalents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/09—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells
    • C12N2502/094—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells keratinocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1323—Adult fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C12N2503/04—Screening or testing on artificial tissues
    • C12N2503/06—Screening or testing on artificial skin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2539/00—Supports and/or coatings for cell culture characterised by properties
    • C12N2539/10—Coating allowing for selective detachment of cells, e.g. thermoreactive coating

Definitions

  • the present invention relates to novel methods of culturing anchor-dependent cells on microsupports with two-dimensional cryosensitive geometry. More specifically, the invention relates to methods for obtaining anchored-dependent animal cells, individualized or forming cellular associations of leaf types, aggregates, spheroids or more organized organoids, having particular properties such as high cell viability. and homogeneity in size of the cell clusters.
  • the cell sheets recovered in step a) are preferably homodisperse in size, and the size of the spheroids or organoids obtained is controlled. This size essentially depends on the size of the microcarriers, and on the morphology of the cultured cells.
  • organoids can be produced by a method comprising the following steps: a) obtaining spheroids or organoids by a method as described above, b) inoculating these spheroids or organoids with other

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Diabetes (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Dermatology (AREA)
  • Endocrinology (AREA)
  • Obesity (AREA)
  • Hematology (AREA)
  • Emergency Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
EP01927982A 2000-04-18 2001-04-17 Zellkukturverfahren, zellmassen so hergestellt und ihre verwendung Withdrawn EP1280888A1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR0005008A FR2807765B1 (fr) 2000-04-18 2000-04-18 Procede de culture de cellules, amas cellulaires obtenus par ces procedes et utilisation de ceux-ci
FR0005008 2000-04-18
PCT/FR2001/001177 WO2001079453A1 (fr) 2000-04-18 2001-04-17 Procedes de culture de cellules, amas cellulaires obtenus par ces procedes et utilisation de ceux-ci

Publications (1)

Publication Number Publication Date
EP1280888A1 true EP1280888A1 (de) 2003-02-05

Family

ID=8849393

Family Applications (1)

Application Number Title Priority Date Filing Date
EP01927982A Withdrawn EP1280888A1 (de) 2000-04-18 2001-04-17 Zellkukturverfahren, zellmassen so hergestellt und ihre verwendung

Country Status (4)

Country Link
EP (1) EP1280888A1 (de)
AU (1) AU2001254864A1 (de)
FR (1) FR2807765B1 (de)
WO (1) WO2001079453A1 (de)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2657013A1 (en) * 2006-07-06 2008-01-10 Organogenesis Inc. Temperature-responsive microcarrier
FI20065514A0 (fi) * 2006-08-16 2006-08-16 Licentia Oy Aktivoidut fibroblastit kudosvaurioiden hoitamiseksi
US10108980B2 (en) * 2011-06-24 2018-10-23 At&T Intellectual Property I, L.P. Method and apparatus for targeted advertising
FR3027917B1 (fr) * 2014-10-30 2016-12-30 Syntivia Spheroides mixtes de melanocytes et de keratinocytes
CN114410466A (zh) * 2020-04-01 2022-04-29 沈西凌 用于个性化癌症治疗的精确药物筛选

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1095967A1 (de) * 1999-10-29 2001-05-02 Computer Cell Culture Center S.A. Neue Zellkulturträger mit besonderen Eigenschaften und ihre Herstellung

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0355112A1 (de) * 1987-04-22 1990-02-28 Michael Bay Zellkulturverfahren, -materialien und -produkte
EP0470681A3 (en) * 1990-08-08 1992-04-22 W.R. Grace & Co.-Conn. Cell clusters or sheets comprising a plurality of cell species and preparations thereof
EP0499932A1 (de) * 1991-02-16 1992-08-26 W.R. Grace & Co.-Conn. Tumorzellmasse, die Tumorzellen und Normalzellen enthält, und ihre Herstellung
JPH06510899A (ja) * 1991-02-18 1994-12-08 コンピューター　セル　カルチャー　センター 細胞の培養のための支持体およびそのような支持体の製造方法
WO1993003139A1 (fr) * 1991-08-08 1993-02-18 Kao Corporation Support pour la culture de cellules, production de ce support et production d'un amas de cellules au moyen de ce support
IL115728A0 (en) * 1994-10-25 1996-01-19 Boehringer Mannheim Gmbh Biomaterial containing epithelial cells and use thereof as a transplant

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1095967A1 (de) * 1999-10-29 2001-05-02 Computer Cell Culture Center S.A. Neue Zellkulturträger mit besonderen Eigenschaften und ihre Herstellung

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of WO0179453A1 *

Also Published As

Publication number Publication date
WO2001079453A1 (fr) 2001-10-25
FR2807765A1 (fr) 2001-10-19
FR2807765B1 (fr) 2004-12-31
AU2001254864A1 (en) 2001-10-30

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PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

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Effective date: 20080118

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Owner name: LA REGION WALLONNE

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Effective date: 20100901