EP1283893A2 - Region regulatrice de gene qui favorise la transcription specifique de la racine et utilisation de cette region - Google Patents
Region regulatrice de gene qui favorise la transcription specifique de la racine et utilisation de cette regionInfo
- Publication number
- EP1283893A2 EP1283893A2 EP01940917A EP01940917A EP1283893A2 EP 1283893 A2 EP1283893 A2 EP 1283893A2 EP 01940917 A EP01940917 A EP 01940917A EP 01940917 A EP01940917 A EP 01940917A EP 1283893 A2 EP1283893 A2 EP 1283893A2
- Authority
- EP
- European Patent Office
- Prior art keywords
- nucleic acid
- sequence
- plants
- isolated nucleic
- roots
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 32
- 230000035897 transcription Effects 0.000 title claims abstract description 11
- 238000013518 transcription Methods 0.000 title claims abstract description 11
- 230000001105 regulatory effect Effects 0.000 title description 6
- 150000007523 nucleic acids Chemical group 0.000 claims abstract description 29
- 230000014509 gene expression Effects 0.000 claims abstract description 20
- 108091028043 Nucleic acid sequence Proteins 0.000 claims abstract description 18
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 claims abstract description 7
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 claims abstract description 7
- 238000004519 manufacturing process Methods 0.000 claims abstract description 7
- 201000010099 disease Diseases 0.000 claims abstract description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 5
- 230000000050 nutritive effect Effects 0.000 claims abstract description 5
- 241000607479 Yersinia pestis Species 0.000 claims abstract description 4
- 230000002708 enhancing effect Effects 0.000 claims description 4
- 208000015181 infectious disease Diseases 0.000 claims 1
- 230000009261 transgenic effect Effects 0.000 abstract description 9
- 238000009313 farming Methods 0.000 abstract description 4
- 150000003839 salts Chemical class 0.000 abstract description 4
- 241000196324 Embryophyta Species 0.000 description 26
- 102000053187 Glucuronidase Human genes 0.000 description 8
- 108010060309 Glucuronidase Proteins 0.000 description 8
- 241000893896 Physaria fendleri Species 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 108020004414 DNA Proteins 0.000 description 4
- 229910052751 metal Inorganic materials 0.000 description 4
- 239000002184 metal Substances 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
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- 102000004169 proteins and genes Human genes 0.000 description 3
- 239000000523 sample Substances 0.000 description 3
- 241000589158 Agrobacterium Species 0.000 description 2
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 2
- 241000219194 Arabidopsis Species 0.000 description 2
- 108700025350 Arabidopsis Fae1 Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 229940088598 enzyme Drugs 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 238000010353 genetic engineering Methods 0.000 description 2
- 238000009396 hybridization Methods 0.000 description 2
- 229930027917 kanamycin Natural products 0.000 description 2
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 2
- 229960000318 kanamycin Drugs 0.000 description 2
- 229930182823 kanamycin A Natural products 0.000 description 2
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- 229930014626 natural product Natural products 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 239000000137 peptide hydrolase inhibitor Substances 0.000 description 2
- 230000008121 plant development Effects 0.000 description 2
- 239000013612 plasmid Substances 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 230000014493 regulation of gene expression Effects 0.000 description 2
- 229910000162 sodium phosphate Inorganic materials 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- 150000004669 very long chain fatty acids Chemical class 0.000 description 2
- 108020005065 3' Flanking Region Proteins 0.000 description 1
- DHJFFLKPAYHPHU-BYNIDDHOSA-N 5-bromo-4-chloro-3-indolyl beta-D-glucuronide Chemical compound O1[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1OC1=CNC2=CC=C(Br)C(Cl)=C12 DHJFFLKPAYHPHU-BYNIDDHOSA-N 0.000 description 1
- 101100126948 Arabidopsis thaliana FAE1 gene Proteins 0.000 description 1
- 241000972773 Aulopiformes Species 0.000 description 1
- 241000193388 Bacillus thuringiensis Species 0.000 description 1
- 101710132601 Capsid protein Proteins 0.000 description 1
- 102000012286 Chitinases Human genes 0.000 description 1
- 108010022172 Chitinases Proteins 0.000 description 1
- 101710094648 Coat protein Proteins 0.000 description 1
- 102000029816 Collagenase Human genes 0.000 description 1
- 108060005980 Collagenase Proteins 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 102100021181 Golgi phosphoprotein 3 Human genes 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 102000004856 Lectins Human genes 0.000 description 1
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- 101710125418 Major capsid protein Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
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- 101710141454 Nucleoprotein Proteins 0.000 description 1
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- 229940097012 bacillus thuringiensis Drugs 0.000 description 1
- 229920000704 biodegradable plastic Polymers 0.000 description 1
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- 150000001875 compounds Chemical class 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 1
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 1
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 1
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 1
- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 238000010230 functional analysis Methods 0.000 description 1
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- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 230000001744 histochemical effect Effects 0.000 description 1
- 230000000749 insecticidal effect Effects 0.000 description 1
- 239000002523 lectin Substances 0.000 description 1
- 230000004807 localization Effects 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 108010058731 nopaline synthase Proteins 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- -1 potassium ferricyanide Chemical compound 0.000 description 1
- 239000000276 potassium ferrocyanide Substances 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
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- 229930000044 secondary metabolite Natural products 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- XOGGUFAVLNCTRS-UHFFFAOYSA-N tetrapotassium;iron(2+);hexacyanide Chemical compound [K+].[K+].[K+].[K+].[Fe+2].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-] XOGGUFAVLNCTRS-UHFFFAOYSA-N 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
- C12N15/8223—Vegetative tissue-specific promoters
- C12N15/8227—Root-specific
Definitions
- Figure 1 shows the sequence of the insert in the plasmid pLfKCS3-GUS.
- the present invention provides a nucleic acid sequence which directs root-specific transcription of contiguous genes in plants.
- the present invention shows that an isolated transcription regulatory region from the Lf CS45 gene is capable of directing expression of desired genes in a root-specific manner.
- this regulatory sequence can also promote transcription in roots of a different plant species, it is useful for a number of different applications in a variety of dicotyledonous plants. Examples of applications wherein the nucleic acid sequence of the present invention can be useful includes for example:
- the sequence of the insert in the plasmid pLfKCS3-GUS is shown in Figure 1.
- the pLfKCS45-GUS construct was introduced into Agrobacterium tumefaciens strain GN3101 (pMP90) (Koncz and Schell, 1986) by heat shock and selected for resistance to kanamycin (50 [ ⁇ g/mL).
- A. thaliana ecotype Columbia was transformed with A. tumefaciens harbouring the pLfKCS45-GUS construct using floral dip method (Clough and Bent, 1998). Screening for transformed seed was done on 50 [ ⁇ g/mL kanamycin as described previously (Katavic et al., 1994).
- transgenic lines were generated and examined for GUS activity.
- Histochemical localization of GUS activity in transgenic plants was done in different tissues as follows. Tissue sections were incubated in 50 mM sodium phosphate, pH 7.0, 0.5 mM potassium ferricyanide, 0.5 mM potassium ferrocyanide, 10 mM EDTA, 0.05%(w/v) triton X-100, and 0.35 mg/ml 5-bromo-4-chloro-3-indolyl- ⁇ -D-glucuronide (X-Gluc) for 4 to 7 hours at 37°C (Jefferson, 1987). Following staining the blue-stained samples were fixed in 70% ethanol.
- LfKCS45 promoter is useful for root-specific expression of foreign genes in transgenic plants and is capable of promoting transcription in plants other than Lesquerella fendleri.
- LTKCS45 promoter root-specific expression conferred by the LTKCS45 promoter is independent of the native terminator at the LJKCS45 gene 3' end.
- a terminator derived from the Agrobacterium nopaline synthase gene was used.
- sequence in the 1025 bp promoter construct is sufficient for the desired expression profile independent of ancillary sequences.
- T L -DNA gene 5 controls the tissue-specific expression of chimaeric genes carried by a novel type of Agrobacterium binary vector. Mol. Gen. Genet. 204: 383-396.
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
La présente invention concerne une séquence d'acide nucléique qui dirige la transcription spécifique de la racine de gènes contigus dans des plantes. Cette séquence peut être utilisée dans des plantes transgéniques pour favoriser l'expression de gènes endogènes et étrangers dans des racines de façon à renforcer la résistance de la plante aux maladies et aux parasites, à renforcer la tolérance au stress (sel), et à améliorer la valeur nutritive des plantes à racines comestibles. De plus, cette séquence peut être utile pour la production de protéines de recombinaison dans des racines destinées à la culture moléculaire et à la phytorestauration.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US20678800P | 2000-05-24 | 2000-05-24 | |
| US206788P | 2000-05-24 | ||
| PCT/IB2001/001137 WO2001090387A2 (fr) | 2000-05-24 | 2001-05-24 | Region regulatrice de gene qui favorise la transcription specifique de la racine et utilisation de cette region |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| EP1283893A2 true EP1283893A2 (fr) | 2003-02-19 |
Family
ID=22767963
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP01940917A Withdrawn EP1283893A2 (fr) | 2000-05-24 | 2001-05-24 | Region regulatrice de gene qui favorise la transcription specifique de la racine et utilisation de cette region |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP1283893A2 (fr) |
| AU (1) | AU2001274405A1 (fr) |
| BR (1) | BR0111081A (fr) |
| CA (1) | CA2409881A1 (fr) |
| WO (1) | WO2001090387A2 (fr) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117101709B (zh) * | 2023-07-25 | 2025-11-28 | 大连理工大学 | 一种双载体负载金属氧化物催化剂的制备方法及其应用 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1995007357A2 (fr) * | 1993-09-04 | 1995-03-16 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Promoteurs |
| US5837876A (en) * | 1995-07-28 | 1998-11-17 | North Carolina State University | Root cortex specific gene promoter |
| JP3791059B2 (ja) * | 1996-08-12 | 2006-06-28 | 住友化学株式会社 | 植物プロモーターおよびその利用 |
| AU750707C (en) * | 1997-04-14 | 2003-05-15 | University Of British Columbia, The | Nucleic acids encoding a plant enzyme involved in very long chain fatty acid synthesis |
| JP2004527201A (ja) * | 1998-04-09 | 2004-09-09 | アリゾナ ボード オブ リーゼンツ | アルフィン１の発現、ならびに根の生長および根の特定の遺伝子活性化の増大したトランスジェニック植物を生産する方法 |
| GB9808304D0 (en) * | 1998-04-20 | 1998-06-17 | Zeneca Ltd | Improvements in or relating to organic compounds |
| US20040049806A1 (en) * | 2000-05-24 | 2004-03-11 | Ljerka Kunst | Nucleic acid encoding a plant very long chain fatty acid biosynthetic enzyme |
-
2001
- 2001-05-24 BR BR0111081-0A patent/BR0111081A/pt not_active Application Discontinuation
- 2001-05-24 WO PCT/IB2001/001137 patent/WO2001090387A2/fr not_active Ceased
- 2001-05-24 CA CA002409881A patent/CA2409881A1/fr not_active Abandoned
- 2001-05-24 EP EP01940917A patent/EP1283893A2/fr not_active Withdrawn
- 2001-05-24 AU AU2001274405A patent/AU2001274405A1/en not_active Abandoned
Non-Patent Citations (1)
| Title |
|---|
| See references of WO0190387A3 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CA2409881A1 (fr) | 2001-11-29 |
| WO2001090387A3 (fr) | 2002-06-27 |
| AU2001274405A1 (en) | 2001-12-03 |
| WO2001090387A2 (fr) | 2001-11-29 |
| BR0111081A (pt) | 2003-04-08 |
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| PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
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| STAA | Information on the status of an ep patent application or granted ep patent |
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Effective date: 20021129 |
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| AK | Designated contracting states |
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| AX | Request for extension of the european patent |
Extension state: AL LT LV MK RO SI |
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| 18W | Application withdrawn |
Effective date: 20030104 |