EP1287404A4 - Procede d'immobilisation d'oligonucleotides au moyen du procede de bioconjugaison de cycloaddition - Google Patents

Procede d'immobilisation d'oligonucleotides au moyen du procede de bioconjugaison de cycloaddition

Info

Publication number
EP1287404A4
EP1287404A4 EP01932784A EP01932784A EP1287404A4 EP 1287404 A4 EP1287404 A4 EP 1287404A4 EP 01932784 A EP01932784 A EP 01932784A EP 01932784 A EP01932784 A EP 01932784A EP 1287404 A4 EP1287404 A4 EP 1287404A4
Authority
EP
European Patent Office
Prior art keywords
cycloaddition
immobilizing oligonucleotides
bioconjugation process
bioconjugation
immobilizing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP01932784A
Other languages
German (de)
English (en)
Other versions
EP1287404A1 (fr
Inventor
Wolfgang Pieken
Andreas Wolter
David P Sebesta
Michael Leuck
Hallie A Latham-Timmons
John Pilon
Gregory M Husar
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sigma Aldrich Co LLC
Original Assignee
Proligo LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Proligo LLC filed Critical Proligo LLC
Publication of EP1287404A1 publication Critical patent/EP1287404A1/fr
Publication of EP1287404A4 publication Critical patent/EP1287404A4/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00Libraries per se, e.g. arrays, mixtures
    • C40B40/04Libraries containing only organic compounds
    • C40B40/06Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07CACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C215/00Compounds containing amino and hydroxy groups bound to the same carbon skeleton
    • C07C215/02Compounds containing amino and hydroxy groups bound to the same carbon skeleton having hydroxy groups and amino groups bound to acyclic carbon atoms of the same carbon skeleton
    • C07C215/04Compounds containing amino and hydroxy groups bound to the same carbon skeleton having hydroxy groups and amino groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being saturated
    • C07C215/06Compounds containing amino and hydroxy groups bound to the same carbon skeleton having hydroxy groups and amino groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being saturated and acyclic
    • C07C215/10Compounds containing amino and hydroxy groups bound to the same carbon skeleton having hydroxy groups and amino groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being saturated and acyclic with one amino group and at least two hydroxy groups bound to the carbon skeleton
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07CACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C271/00Derivatives of carbamic acids, i.e. compounds containing any of the groups, the nitrogen atom not being part of nitro or nitroso groups
    • C07C271/06Esters of carbamic acids
    • C07C271/08Esters of carbamic acids having oxygen atoms of carbamate groups bound to acyclic carbon atoms
    • C07C271/10Esters of carbamic acids having oxygen atoms of carbamate groups bound to acyclic carbon atoms with the nitrogen atoms of the carbamate groups bound to hydrogen atoms or to acyclic carbon atoms
    • C07C271/16Esters of carbamic acids having oxygen atoms of carbamate groups bound to acyclic carbon atoms with the nitrogen atoms of the carbamate groups bound to hydrogen atoms or to acyclic carbon atoms to carbon atoms of hydrocarbon radicals substituted by singly-bound oxygen atoms
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07DHETEROCYCLIC COMPOUNDS
    • C07D207/00Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom
    • C07D207/46Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom with hetero atoms directly attached to the ring nitrogen atom
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07FACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F7/00Compounds containing elements of Groups 4 or 14 of the Periodic Table
    • C07F7/02Silicon compounds
    • C07F7/08Compounds having one or more C—Si linkages
    • C07F7/18Compounds having one or more C—Si linkages as well as one or more C—O—Si linkages
    • C07F7/1804Compounds having Si-O-C linkages
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07FACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02Phosphorus compounds
    • C07F9/06Phosphorus compounds without P—C bonds
    • C07F9/22Amides of acids of phosphorus
    • C07F9/24Esteramides
    • C07F9/2404Esteramides the ester moiety containing a substituent or a structure which is considered as characteristic
    • C07F9/2408Esteramides the ester moiety containing a substituent or a structure which is considered as characteristic of hydroxyalkyl compounds
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/14Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
    • C40B50/18Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support using a particular method of attachment to the solid support
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07BGENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
    • C07B2200/00Indexing scheme relating to specific properties of organic compounds
    • C07B2200/11Compounds covalently bound to a solid support
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07CACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C2601/00Systems containing only non-condensed rings
    • C07C2601/12Systems containing only non-condensed rings with a six-membered ring
    • C07C2601/16Systems containing only non-condensed rings with a six-membered ring the ring being unsaturated

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Structural Engineering (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Saccharide Compounds (AREA)
  • Indole Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Pyrrole Compounds (AREA)
EP01932784A 2000-05-01 2001-05-01 Procede d'immobilisation d'oligonucleotides au moyen du procede de bioconjugaison de cycloaddition Withdrawn EP1287404A4 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US20156100P 2000-05-01 2000-05-01
US201561P 2000-05-01
US26502001P 2001-01-30 2001-01-30
US265020P 2001-01-30
PCT/US2001/013956 WO2001084234A1 (fr) 2000-05-01 2001-05-01 Procede d'immobilisation d'oligonucleotides au moyen du procede de bioconjugaison de cycloaddition

Publications (2)

Publication Number Publication Date
EP1287404A1 EP1287404A1 (fr) 2003-03-05
EP1287404A4 true EP1287404A4 (fr) 2003-10-15

Family

ID=26896865

Family Applications (1)

Application Number Title Priority Date Filing Date
EP01932784A Withdrawn EP1287404A4 (fr) 2000-05-01 2001-05-01 Procede d'immobilisation d'oligonucleotides au moyen du procede de bioconjugaison de cycloaddition

Country Status (4)

Country Link
EP (1) EP1287404A4 (fr)
JP (1) JP2003535317A (fr)
AU (1) AU2001259285A1 (fr)
WO (1) WO2001084234A1 (fr)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7427678B2 (en) 1998-01-08 2008-09-23 Sigma-Aldrich Co. Method for immobilizing oligonucleotides employing the cycloaddition bioconjugation method
US6664061B2 (en) 1999-06-25 2003-12-16 Amersham Biosciences Ab Use and evaluation of a [2+2] photoaddition in immobilization of oligonucleotides on a three-dimensional hydrogel matrix
AU2001279244A1 (en) * 2000-08-09 2002-02-18 Motorola, Inc. The use and evaluation of a (2+2) photocycloaddition in immobilization of oligonucleotides on a three-dimensional hydrogel matrix
US7098326B2 (en) 2002-01-23 2006-08-29 Sigma-Aldrich Co. Methods for the integrated synthesis and purification of oligonucleotides
JP4791043B2 (ja) * 2002-12-31 2011-10-12 プロリゴ・エルエルシー 同一固体支持体上で、2以上のオリゴヌクレオチドをタンデムに合成するための方法および組成物
US7790835B2 (en) 2003-12-03 2010-09-07 Nektar Therapeutics Method of preparing maleimide functionalized polymers
EP1697749B1 (fr) * 2003-12-22 2013-04-17 Imec Utilisation de structures micro-electronique pour le depot de molecules sur des surfaces selon un trace
JPWO2005085857A1 (ja) * 2004-03-05 2008-01-24 日清紡績株式会社 固定化生体分子及び生体分子と相互作用し得る物質の検出法
JP4581571B2 (ja) * 2004-09-06 2010-11-17 富士ゼロックス株式会社 粒子のマレイミジル基量を測定する定量方法
DE602006013151D1 (de) 2005-07-19 2010-05-06 Nektar Therapeutics Verfahren zur herstellung von polymermaleimiden
GB0619182D0 (en) * 2006-09-29 2006-11-08 Leuven K U Res & Dev Oligonucleotide arrays
JP5682311B2 (ja) * 2008-12-02 2015-03-11 和光純薬工業株式会社 光塩基発生剤
CN104321431A (zh) * 2012-05-21 2015-01-28 安捷伦科技有限公司 在dna/rna应用中作为可裂解接头的逆狄尔斯阿尔德反应
JP5986872B2 (ja) * 2012-10-05 2016-09-06 公益財団法人微生物化学研究会 化合物、該化合物の製造方法、アセテート誘導体の製造方法、及びアトルバスタチンの製造方法、並びに不斉配位子の回収方法
DK3017065T3 (en) 2013-07-01 2018-11-26 Illumina Inc Catalyst-free Surface functionalization and polymer grafting
US10781175B2 (en) 2016-07-15 2020-09-22 Am Chemicals Llc Solid supports and phosphoramidite building blocks for oligonucleotide conjugates
CN108911941A (zh) * 2018-05-25 2018-11-30 凯莱英生命科学技术(天津)有限公司 1,3-环己二烯连续化合成方法
US20250215041A1 (en) * 2023-12-29 2025-07-03 Life Technologies Corporation System and Method for Aqueous Conjugation of Substrates Using Diels-Alder Chemistry

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998030578A1 (fr) * 1997-01-08 1998-07-16 Proligo Llc Procede de synthese en phase solution d'oligonucleotides et de peptides
WO1998047910A1 (fr) * 1997-04-21 1998-10-29 Proligo Llc Synthese d'oligonucleotides en solution
US6171797B1 (en) * 1999-10-20 2001-01-09 Agilent Technologies Inc. Methods of making polymeric arrays

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2154444A1 (fr) * 1993-01-21 1994-08-04 John F. W. Keana Fonctionalisation chimique des surfaces
EP2319854B1 (fr) * 1997-01-08 2016-11-30 Sigma-Aldrich Co. LLC Bio-conjugaison de macromolécules

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998030578A1 (fr) * 1997-01-08 1998-07-16 Proligo Llc Procede de synthese en phase solution d'oligonucleotides et de peptides
WO1998047910A1 (fr) * 1997-04-21 1998-10-29 Proligo Llc Synthese d'oligonucleotides en solution
US6171797B1 (en) * 1999-10-20 2001-01-09 Agilent Technologies Inc. Methods of making polymeric arrays

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of WO0184234A1 *

Also Published As

Publication number Publication date
AU2001259285A1 (en) 2001-11-12
JP2003535317A (ja) 2003-11-25
WO2001084234A1 (fr) 2001-11-08
EP1287404A1 (fr) 2003-03-05

Similar Documents

Publication Publication Date Title
EP1287404A4 (fr) Procede d'immobilisation d'oligonucleotides au moyen du procede de bioconjugaison de cycloaddition
DZ3373A1 (fr) Catalyseur et procede de preparation d'hydrocarbures
CH1143977H1 (fr) 2-Oxy-benzoxazine-4-ones destinees au traitement de l'obesite
DE60042984D1 (de) Arzneitmittel zur Behandlung von Duchenne-Muskeldystrophie
PL358451A1 (pl) Sposób sterylizacji materiałów biologicznych
DE60138391D1 (de) Vorrichtung und kits zum feststellen und desinfizieren eingesetzter katheter
NO20014957L (no) Fremgangsmåte for fremstilling av aminplatinakomplekser
FR2799554B1 (fr) Composition d'elimination de photoresist et procede pour eliminer un photoresist au moyen de cette composition
NO20021964L (no) Biologisk fluidbehandling
EP1710308A4 (fr) Procede permettant de renforcer l'activite d'un anticorps
BR0106411B1 (pt) processo de tratamento do lìquido residual de cromatização.
ATE355067T1 (de) 15-keto-prostaglandine zur behandlung arzneimittelinduzierter obstipation
NO20032822L (no) Fremgangsmate for konsentrering av brukt syre
DE60035326D1 (de) Verfahren zur Behandlung von Abwasser
DE69835419D1 (de) Verfahren zur Behandlung von organischem Abwasser
DE60132390D1 (de) Biosensor und Verfahren zu seiner Herstellung
NO20022282D0 (no) Fremgangsmåte for fremstilling av biopolymerer av nitrogenfattig avlöpsvann
EA200101260A1 (ru) Способ изменения физико-химических и/или биологических свойств вещества
NO20023454L (no) Beisemiddel inneholdende urea og fremgangsmåte for fremstilling av dette
FR2818979B1 (fr) Procede de preparation d'imidaclopride
DE59915132D1 (de) Lithographieverfahren
SE9904506D0 (sv) Method for immobilisation
FR2818287B1 (fr) Support solide pour l'immobilisation d'oligonucleotides
FR2806085B1 (fr) Procede de preparation du citalopram
DE60005685D1 (de) Oxazinocarbazole zur behandlung von cns-erkrankungen

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20021129

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR

AX Request for extension of the european patent

Extension state: AL LT LV MK RO SI

RIC1 Information provided on ipc code assigned before grant

Ipc: 7C 07H 21/00 B

Ipc: 7C 07F 9/24 B

Ipc: 7C 07F 7/18 B

Ipc: 7C 07D 207/46 B

Ipc: 7C 07C 271/16 B

Ipc: 7C 07C 215/10 B

Ipc: 7C 07B 61/00 B

Ipc: 7C 07H 19/04 B

Ipc: 7C 07H 19/00 A

A4 Supplementary search report drawn up and despatched

Effective date: 20030827

17Q First examination report despatched

Effective date: 20040114

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20040525