EP1287404A4 - Procede d'immobilisation d'oligonucleotides au moyen du procede de bioconjugaison de cycloaddition - Google Patents
Procede d'immobilisation d'oligonucleotides au moyen du procede de bioconjugaison de cycloadditionInfo
- Publication number
- EP1287404A4 EP1287404A4 EP01932784A EP01932784A EP1287404A4 EP 1287404 A4 EP1287404 A4 EP 1287404A4 EP 01932784 A EP01932784 A EP 01932784A EP 01932784 A EP01932784 A EP 01932784A EP 1287404 A4 EP1287404 A4 EP 1287404A4
- Authority
- EP
- European Patent Office
- Prior art keywords
- cycloaddition
- immobilizing oligonucleotides
- bioconjugation process
- bioconjugation
- immobilizing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 238000000034 method Methods 0.000 title 2
- 108091034117 Oligonucleotide Proteins 0.000 title 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 title 1
- 238000006352 cycloaddition reaction Methods 0.000 title 1
- 230000003100 immobilizing effect Effects 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C215/00—Compounds containing amino and hydroxy groups bound to the same carbon skeleton
- C07C215/02—Compounds containing amino and hydroxy groups bound to the same carbon skeleton having hydroxy groups and amino groups bound to acyclic carbon atoms of the same carbon skeleton
- C07C215/04—Compounds containing amino and hydroxy groups bound to the same carbon skeleton having hydroxy groups and amino groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being saturated
- C07C215/06—Compounds containing amino and hydroxy groups bound to the same carbon skeleton having hydroxy groups and amino groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being saturated and acyclic
- C07C215/10—Compounds containing amino and hydroxy groups bound to the same carbon skeleton having hydroxy groups and amino groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being saturated and acyclic with one amino group and at least two hydroxy groups bound to the carbon skeleton
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C271/00—Derivatives of carbamic acids, i.e. compounds containing any of the groups, the nitrogen atom not being part of nitro or nitroso groups
- C07C271/06—Esters of carbamic acids
- C07C271/08—Esters of carbamic acids having oxygen atoms of carbamate groups bound to acyclic carbon atoms
- C07C271/10—Esters of carbamic acids having oxygen atoms of carbamate groups bound to acyclic carbon atoms with the nitrogen atoms of the carbamate groups bound to hydrogen atoms or to acyclic carbon atoms
- C07C271/16—Esters of carbamic acids having oxygen atoms of carbamate groups bound to acyclic carbon atoms with the nitrogen atoms of the carbamate groups bound to hydrogen atoms or to acyclic carbon atoms to carbon atoms of hydrocarbon radicals substituted by singly-bound oxygen atoms
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D207/00—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom
- C07D207/46—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom with hetero atoms directly attached to the ring nitrogen atom
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F7/00—Compounds containing elements of Groups 4 or 14 of the Periodic Table
- C07F7/02—Silicon compounds
- C07F7/08—Compounds having one or more C—Si linkages
- C07F7/18—Compounds having one or more C—Si linkages as well as one or more C—O—Si linkages
- C07F7/1804—Compounds having Si-O-C linkages
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
- C07F9/02—Phosphorus compounds
- C07F9/06—Phosphorus compounds without P—C bonds
- C07F9/22—Amides of acids of phosphorus
- C07F9/24—Esteramides
- C07F9/2404—Esteramides the ester moiety containing a substituent or a structure which is considered as characteristic
- C07F9/2408—Esteramides the ester moiety containing a substituent or a structure which is considered as characteristic of hydroxyalkyl compounds
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/14—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
- C40B50/18—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support using a particular method of attachment to the solid support
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
- C07B2200/00—Indexing scheme relating to specific properties of organic compounds
- C07B2200/11—Compounds covalently bound to a solid support
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C2601/00—Systems containing only non-condensed rings
- C07C2601/12—Systems containing only non-condensed rings with a six-membered ring
- C07C2601/16—Systems containing only non-condensed rings with a six-membered ring the ring being unsaturated
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Structural Engineering (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Saccharide Compounds (AREA)
- Indole Compounds (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Pyrrole Compounds (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US20156100P | 2000-05-01 | 2000-05-01 | |
| US201561P | 2000-05-01 | ||
| US26502001P | 2001-01-30 | 2001-01-30 | |
| US265020P | 2001-01-30 | ||
| PCT/US2001/013956 WO2001084234A1 (fr) | 2000-05-01 | 2001-05-01 | Procede d'immobilisation d'oligonucleotides au moyen du procede de bioconjugaison de cycloaddition |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| EP1287404A1 EP1287404A1 (fr) | 2003-03-05 |
| EP1287404A4 true EP1287404A4 (fr) | 2003-10-15 |
Family
ID=26896865
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP01932784A Withdrawn EP1287404A4 (fr) | 2000-05-01 | 2001-05-01 | Procede d'immobilisation d'oligonucleotides au moyen du procede de bioconjugaison de cycloaddition |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP1287404A4 (fr) |
| JP (1) | JP2003535317A (fr) |
| AU (1) | AU2001259285A1 (fr) |
| WO (1) | WO2001084234A1 (fr) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7427678B2 (en) | 1998-01-08 | 2008-09-23 | Sigma-Aldrich Co. | Method for immobilizing oligonucleotides employing the cycloaddition bioconjugation method |
| US6664061B2 (en) | 1999-06-25 | 2003-12-16 | Amersham Biosciences Ab | Use and evaluation of a [2+2] photoaddition in immobilization of oligonucleotides on a three-dimensional hydrogel matrix |
| AU2001279244A1 (en) * | 2000-08-09 | 2002-02-18 | Motorola, Inc. | The use and evaluation of a (2+2) photocycloaddition in immobilization of oligonucleotides on a three-dimensional hydrogel matrix |
| US7098326B2 (en) | 2002-01-23 | 2006-08-29 | Sigma-Aldrich Co. | Methods for the integrated synthesis and purification of oligonucleotides |
| JP4791043B2 (ja) * | 2002-12-31 | 2011-10-12 | プロリゴ・エルエルシー | 同一固体支持体上で、2以上のオリゴヌクレオチドをタンデムに合成するための方法および組成物 |
| US7790835B2 (en) | 2003-12-03 | 2010-09-07 | Nektar Therapeutics | Method of preparing maleimide functionalized polymers |
| EP1697749B1 (fr) * | 2003-12-22 | 2013-04-17 | Imec | Utilisation de structures micro-electronique pour le depot de molecules sur des surfaces selon un trace |
| JPWO2005085857A1 (ja) * | 2004-03-05 | 2008-01-24 | 日清紡績株式会社 | 固定化生体分子及び生体分子と相互作用し得る物質の検出法 |
| JP4581571B2 (ja) * | 2004-09-06 | 2010-11-17 | 富士ゼロックス株式会社 | 粒子のマレイミジル基量を測定する定量方法 |
| DE602006013151D1 (de) | 2005-07-19 | 2010-05-06 | Nektar Therapeutics | Verfahren zur herstellung von polymermaleimiden |
| GB0619182D0 (en) * | 2006-09-29 | 2006-11-08 | Leuven K U Res & Dev | Oligonucleotide arrays |
| JP5682311B2 (ja) * | 2008-12-02 | 2015-03-11 | 和光純薬工業株式会社 | 光塩基発生剤 |
| CN104321431A (zh) * | 2012-05-21 | 2015-01-28 | 安捷伦科技有限公司 | 在dna/rna应用中作为可裂解接头的逆狄尔斯阿尔德反应 |
| JP5986872B2 (ja) * | 2012-10-05 | 2016-09-06 | 公益財団法人微生物化学研究会 | 化合物、該化合物の製造方法、アセテート誘導体の製造方法、及びアトルバスタチンの製造方法、並びに不斉配位子の回収方法 |
| DK3017065T3 (en) | 2013-07-01 | 2018-11-26 | Illumina Inc | Catalyst-free Surface functionalization and polymer grafting |
| US10781175B2 (en) | 2016-07-15 | 2020-09-22 | Am Chemicals Llc | Solid supports and phosphoramidite building blocks for oligonucleotide conjugates |
| CN108911941A (zh) * | 2018-05-25 | 2018-11-30 | 凯莱英生命科学技术(天津)有限公司 | 1,3-环己二烯连续化合成方法 |
| US20250215041A1 (en) * | 2023-12-29 | 2025-07-03 | Life Technologies Corporation | System and Method for Aqueous Conjugation of Substrates Using Diels-Alder Chemistry |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998030578A1 (fr) * | 1997-01-08 | 1998-07-16 | Proligo Llc | Procede de synthese en phase solution d'oligonucleotides et de peptides |
| WO1998047910A1 (fr) * | 1997-04-21 | 1998-10-29 | Proligo Llc | Synthese d'oligonucleotides en solution |
| US6171797B1 (en) * | 1999-10-20 | 2001-01-09 | Agilent Technologies Inc. | Methods of making polymeric arrays |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2154444A1 (fr) * | 1993-01-21 | 1994-08-04 | John F. W. Keana | Fonctionalisation chimique des surfaces |
| EP2319854B1 (fr) * | 1997-01-08 | 2016-11-30 | Sigma-Aldrich Co. LLC | Bio-conjugaison de macromolécules |
-
2001
- 2001-05-01 JP JP2001580595A patent/JP2003535317A/ja not_active Ceased
- 2001-05-01 WO PCT/US2001/013956 patent/WO2001084234A1/fr not_active Ceased
- 2001-05-01 EP EP01932784A patent/EP1287404A4/fr not_active Withdrawn
- 2001-05-01 AU AU2001259285A patent/AU2001259285A1/en not_active Abandoned
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998030578A1 (fr) * | 1997-01-08 | 1998-07-16 | Proligo Llc | Procede de synthese en phase solution d'oligonucleotides et de peptides |
| WO1998047910A1 (fr) * | 1997-04-21 | 1998-10-29 | Proligo Llc | Synthese d'oligonucleotides en solution |
| US6171797B1 (en) * | 1999-10-20 | 2001-01-09 | Agilent Technologies Inc. | Methods of making polymeric arrays |
Non-Patent Citations (1)
| Title |
|---|
| See also references of WO0184234A1 * |
Also Published As
| Publication number | Publication date |
|---|---|
| AU2001259285A1 (en) | 2001-11-12 |
| JP2003535317A (ja) | 2003-11-25 |
| WO2001084234A1 (fr) | 2001-11-08 |
| EP1287404A1 (fr) | 2003-03-05 |
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Legal Events
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| AX | Request for extension of the european patent |
Extension state: AL LT LV MK RO SI |
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| RIC1 | Information provided on ipc code assigned before grant |
Ipc: 7C 07H 21/00 B Ipc: 7C 07F 9/24 B Ipc: 7C 07F 7/18 B Ipc: 7C 07D 207/46 B Ipc: 7C 07C 271/16 B Ipc: 7C 07C 215/10 B Ipc: 7C 07B 61/00 B Ipc: 7C 07H 19/04 B Ipc: 7C 07H 19/00 A |
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| A4 | Supplementary search report drawn up and despatched |
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| 17Q | First examination report despatched |
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| STAA | Information on the status of an ep patent application or granted ep patent |
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| 18D | Application deemed to be withdrawn |
Effective date: 20040525 |