EP1343910A2 - Analyse d'identification d'un compose d'essai - Google Patents

Analyse d'identification d'un compose d'essai

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Publication number
EP1343910A2
EP1343910A2 EP01969049A EP01969049A EP1343910A2 EP 1343910 A2 EP1343910 A2 EP 1343910A2 EP 01969049 A EP01969049 A EP 01969049A EP 01969049 A EP01969049 A EP 01969049A EP 1343910 A2 EP1343910 A2 EP 1343910A2
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EP
European Patent Office
Prior art keywords
rna
target rna
test compound
modification
enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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EP01969049A
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German (de)
English (en)
Inventor
Alastair Iain Hamilton Murchie
Georg Friedrich Lentzen
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Vernalis Cambridge Ltd
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Ribotargets Ltd
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Priority claimed from GB0009772A external-priority patent/GB0009772D0/en
Application filed by Ribotargets Ltd filed Critical Ribotargets Ltd
Publication of EP1343910A2 publication Critical patent/EP1343910A2/fr
Withdrawn legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules

Definitions

  • This invention is in the field of assays for antibiotics and other low molecular weight RNA binding compounds, more particularly assays for identifying compounds that inhibit enzymatic modifications of RNA which result in antibiotic resistance and RNA maturation.
  • antibiotics function by inhibiting protein synthesis, typically by acting at the level of rRNA.
  • the rRNA binding sites of many different types of antibiotics have been mapped by chemical and enzymatic probing to various sub-regions on the 16S and 23 S rRNAs. Examples of antibiotics targeted to these sites include binding of the 16S rRNA A site by members of the aminoglycoside class, binding of the 23S rRNA LI site (the E site) by the oxazolidinone class, and binding of the 23 S rRNA GTPase center by the thiazole class [Spahn & Prescott, 1996] .
  • RNA target of an antibiotic e.g. by methylation
  • modification of the RNA target of an antibiotic can result in resistance [Cundhffe, 1978; Skinner et al, 1983; Clancy et al, 1995].
  • methylation of A- 1067 in the 23 S RNA of Streptomyces azureus results in thiostrepton resistance
  • mono- or di-methylation of the N6 position of A-2058 results in erythromycin resistance.
  • the invention provides a method for determining whether a test compound binds to a target RNA, the method comprising the steps of: (a) contacting the test compound with the target RNA and a RNA-modifying enzyme; and (b) detecting the modification of the target RNA by the enzyme and comparing the amount of modification detected to that of a standard, wherein the comparing determines whether the test compound binds to the target RNA.
  • the target RNA comprises a rRNA or a fragment or sub-region thereof.
  • the target RNA includes a stabilising structure.
  • the target RNA comprise a chemical modification which enhances the stability ofthe target RNA.
  • the RNA-modifying enzyme is selected from the group consisting of a methyltransf erase, a pseudouridine synthase, a guanine glycosylase, a G37-N1- methylguanosine-tRNA-methyltransferase, and a 2'-O-ribosyl phosphate transferase.
  • the methytransferase is the thiostrepton resistance methyltransferase or the erythromycin resistance methyltransferase. It is further preferred that target RNA modification is detected by the incorporation of an isotopic label from S- adenosyl-methionine into the target RNA.
  • the test compound is selected from the group consisting of a peptide, a peptoid, a protein, a lipid, a metal, a nucleotide, a nucleoside, a small organic molecule, and a polyamine.
  • test compound is selected from a combinatorial library.
  • the method is performed in a high-throughput screening format.
  • the invention further encompasses a test compound that binds to a target RNA, the test compound identified by the method of claim 1.
  • the invention further encompasses a kit for determining whether a test compound binds to a target RNA, the kit comprising the target RNA and a RNA-modifying enzyme.
  • the invention further encompasses a method for determining whether a test compound binds to a target RNA, the method comprising the steps of: (a) contacting the test compound with a RNA-modifying enzyme and the target RNA, wherein the target RNA comprises a suicide substrate for the enzyme; and (b) detecting the modification of the enzyme by the suicide substrate, wherein the detecting determines whether the test compound binds to the target RNA.
  • Figure 1 shows various RNA modifications.
  • Figure 2 shows the structural elements of the thiostrepton binding site in the GAR RNA of 23S RNA.
  • Figure 3 shows the results of a methylation experiment carried out around the thiostrepton binding site in the GAR RNA of 23 S RNA.
  • Figure 4 shows the results of testing four additional substrates for methyltransferase.
  • Figure 4A shows testing of the human mGAR (H.s. GAR), a left-hand loop 17-mer fragment of E. coli mGAR (LHL), a left hand loop 29-mer of E. coli mGAR, and the E. coli 60-mer complete mGAR.
  • Figure 4B shows a binding curve for two of the reactions in
  • Figure 5 shows the titration of LI 1 protein in the presence of methyltransferase.
  • Figure 6 shows the titration of thiostrepton in the presence of methyltransferase.
  • Figure 7 shows binding curves for 4 different test compounds.
  • Figure 8 shows the site of action of the erythromycin resistance methyltransferase (erm) in the peptidyl transferase centre.
  • the enzyme dimethylates N6 of A2058 of 23S rRNA.
  • Figure 9 shows the positions of methylation modifications in the decoding site of 16S rRNA that confer resistance to aminoglycoside antibiotics.
  • Figure 10 shows RNA sequences of the thiostrepton binding fragment for a range of bacteria.
  • Figure 11 shows the decoding sites of 16S rRNA for a range of bacteria.
  • Figure 12 shows uridine (grey) residues in 16S rRNA modified by pseudouridine synthase.
  • Figure 13 shows uridine residues (grey) modified by pseudouridine synthase for the 5' half of 23 S rRNA.
  • Figure 14 shows uridine residues (grey) modified by pseudouridine synthase for the 3' half of 23 S rRNA.
  • Figure 15 shows recovery of GAR RNA methylation activity in the presence of Ll l by deletion of the N terminal domain of LI 1.
  • Figure 16 shows the reproducibility of TSR methylase assay.
  • Figure 17 shows interassay variation of the GAR methylation single point assay in a 96- well format.
  • the graph shows the results of two independent assays of the same set of 80 compounds, measured in a single point assay at 50 ⁇ M, plotted against each other.
  • Figure 18 shows methylation of rRNA by Erm methylase.
  • Figure 19 shows the effect of refolding on rRNA methylation by GST-Erm.
  • Figure 20 shows magnesium dependence of rRNA methylation by ERM.
  • Figure 21 shows the decrease in refolded rRNA methylation by ErmE in the presence of erythromycin.
  • Figure 22 shows inhibition of rRNA methylation with ERM by various compounds.
  • the invention provides a method for determining whether a test compound binds to a target RNA, the method comprising the steps of:
  • Inhibition of enzymatic modification of the RNA target indicates that the test compound is capable of binding to the RNA and can thus exhibit antibiotic activity.
  • the target RNA can comprise any RNA which is a substrate of and can be modified by the RNA-modifying enzyme.
  • it is a RNA which can be bound by a known antibiotic and, more preferably, one which is known to be modified in vivo to confer resistance to that antibiotic.
  • the target RNA can be derived from fungal, viral, bacterial (Gram-negative or -positive), or eukaryotic RNA. It can be from a natural source, can be an enzymatically transcribed RNA, or can be synthetic in whole or in part.
  • the target RNA comprises a rRNA (e.g. a 16S or 23S rRNA), or a fragment or sub-region thereof.
  • a rRNA e.g. a 16S or 23S rRNA
  • fragment or sub-region thereof refers to a
  • RNA that comprises a substrate site for a RNA modifying enzyme but comprises fewer than all of the nucleotides of the RNA comprising that sequence as it is expressed in nature.
  • a fragment of sub-region of a target RNA can additionally comprise stabilizing sequences that permit the fragmentor sub-region to adopt an essentially native conformation.
  • Non-limiting examples of suitable target RNAs include the following rRNA sub-regions: the A site in 16S rRNA [Fourmy et al, 1996; Holmes & Cundhffe, 1991]; the spectinomycin site in 16S rRNA [Brink et al., 1994]; the peptidyltransferase centre in 23 S rRNA [Kovalic et al, 1995]; the LI binding site (the E site) in 23S rRNA [Matassova et al, 1999]; the minimal thiostrepton binding fragment of 23 S rRNA, also known as the GTPase activating region (GAR) [Ryan et al, 1991]; a 16S fragment which retains aminoglycoside binding [e.g. Purohit and Stern, 1994; see also US patent 5712096].
  • GAR GTPase activating region
  • the term "essentially native conformation" refers to a conformation of a target RNA that permits the target RNA to be a substrate for the RNA modifying enzyme.
  • a target RNA with an essentially native conformation is modified by a given RNA modifying enzyme at the same site or sites and in the same manner as a native RNA comprising that target RNA sequence or sequences.
  • One of skill in the art can determine if a target RNA adopts an essentially native conformation by exposing the target RNA to the RNA modifying enzyme and detecting modification. If modification, e.g., methylation, occurs on the target RNA at the same site or sites at which a native RNA comprising those target sequences is modified, the target RNA has an essentially native conformation.
  • RNAs in this category include the HIV-1 RRE transcriptional activator region [Zapp et al, 1993], self-splicing group I intron RNA [von Ahsen et al, 1991], mRNA [Bokar et al, 1994], , tRNA [Grosjean et al, 1995], tmRNA [Felden et al, 1998], RNase P RNA and ribozymes [Stage et al, 1995].
  • RNAs include, for example, whole ribosomes, ribosomal subunits, signal recognition particle (SRP) RNA, viroid RNA and RNA viruses. Mimics of these RNAs can also be used.
  • SRP signal recognition particle
  • Target RNA sequences for use in the present invention are typically between 5 and about 3000 nucleotides in length, preferably between 20-100 nucleotides, and most preferably 30-80 nucleotides.
  • the target RNA can comprise a chemically-synthesised oligonucleotide of between 20 and 100 nucleotides in length that is capable of folding to form a secondary structure present in the native rRNA.
  • the target RNA can be formed from a single polynucleotide, folded back on itself to form secondary and tertiary structure, or can be formed from two or more annealed polynucleotides which interact to form secondary and tertiary structure.
  • Examples of the use of two or more oligonucleotides which, after annealing, form a folded target RNA target are given in Kam et al. [WO92/05195; US patent 5786145], describing mimics of the RRE. Synthetic analogues of ribozymes formed by annealing oligonucleotides have also been described [Grasby et al., 1993; Slim et al, 1991].
  • the target RNA can include stabilising or connecting structures (e.g. double- stranded regions, tetraloops, stem-loop structures etc.) added to the target RNA sequence.
  • a stabilising structure is a structure comprised by a sequence or sequences, not normally associated with the target RNA sequence in nature, which permits or assists the target RNA to assume and maintain an essentially native conformation. Examples include, but are not limited to double-stranded regions, tetraloops, and stem-loop and hairpin structures.
  • Connecting structures are regions of complementary nucleotide sequence that hybridize to connect two separate RNAs or two regions of an RNA molecule in order to generate all or part of a target RNA modifying enzyme substrate site.
  • the stability of the target RNA conformation can also be increased by including in the reaction mixture an RNA-binding protein, e.g. a ribosomal protein that is usually associated with the complete RNA substrate.
  • RNA is sensitive to cleavage by cellular ribonucleases, as well as to alkaline or acid conditions, it can be desirable to modify the target RNA to enhance its stability against degradation, or to provide functional groups for immobilising the RNA on solid supports by covalent or non-covalent attachments.
  • chemical modification means a covalent modification (including addition, removal or substitution of chemical groups) of an RNA molecule, in the base, sugar, and/or phosphodiester linkages between sugar moieties that protects the RNA from one or more degrading forces or functionalizes it for an assay format. While it is acknowledged that in the common usage a covalent enzymatic modification is a "chemical modification", as used herein, "chemical modification” is not accomplished with the aid of an enzyme or is acomplihsed as a discrete and separate step.
  • Chemical modifications of the target RNA include, but are not limited to, the following types:
  • pyrimidine derivatives substituted in the 5-position e.g. methyl, bromo, fluoro etc... or replacing a carbonyl group by an amino group
  • purine derivatives lacking specific nitrogen atoms e.g. 7-deaza-adenine, hypoxanthine
  • functionalised in the 8-position e.g. 8-azido adenine, 8-bromo adenine
  • additional functionalities e.g. 2,6-diaminopurine
  • Oligonucleotides covalently linked to reactive functional groups e.g.
  • the term "enhanced stability" means that the half-life of the target RNA is at least 10% longer, and preferably 20% longer, 50% longer, 100% longer, 3-fold longer, or 5-fold, 10-fold, 50-fold, or even 100-fold or more longer for a target RNA comprising a chemical modification than for a target RNA of the same sequence that lacks the modification.
  • RNA half-life is measured, for example, by gel electrophoresis of labelled RNA isolated after various times of incubation under potentially degrading conditions.
  • RNA-associated protein can inhibit the activity of the RNA-modifying enzyme.
  • RNA-modifying activity can in some cases be recovered by deletion of one or more domains of the RNA protein. It is therefore possible to modify the assay such that RNA-associated proteins or fragments of such proteins are present.
  • the RNA-associated proteins or domains thereof, which inhibit the activity of the RNA-modifying enzyme are absent, while RNA-associated proteins or domains thereof required for or beneficial to maintaining the native conformation of the RNA for recognition by the RNA-modifying enzyme, are present.
  • RNA modifications A number of enzymes that covalently modify RNA are known.
  • the modification typically involves the covalent addition to, or alteration of, existing bases in RNA [Limbach et al, 1994; Rozenski et al, 1999]. Modifications usually take place at specific positions i.e. not all bases are modified. Some modifications are shown in Figure 1.
  • RNA modification is methylation, in which a methyl group is transferred by a methyltransferase enzyme from S-adenosyl-methionine (SAM) to a position on the RNA.
  • SAM S-adenosyl-methionine
  • Methyl groups can be introduced at various positions on the bases and also at the 2'-OH position of the ribose.
  • Methyltransferases are also responsible for dimethylation.
  • Methyltransferases include, for example: the E. coli 23S rRNA methyltransferase R ⁇ nJ/FTSJ (Caldas et al, 2000, J. Biol. Chem. 275: 16414-16419); the E.
  • coli 16S rRNA m5C967 methyltransferase (Gu et al, 1999, Biochemistry 38: 4053- 4057); vaccinia virus VP39 2'-O-methyltransferase (Lockless et al., 1998, Biochemistry 37: 8564-8574); lrm, encoding a 26 kDa ribosomal RNA methyltransferase that confers high-level resistance to lincomycin with lower levels of resistance to macrolides (Jenkins et al., 1991, Gene 108: 55-62); the Micromonospora.
  • Pseudouridine synthase reorients an existing uridine to pseudouridine ( ⁇ ) in tRNAs and rRNAs of bacteria and eukaryotes [Foster et al, 2000; Ganot et al, 1997].
  • pseudouridine synthase include, but are not limited to: E. coli truB (Gutgsell et al., 2000, RNA 6: 1870-1881); Schizosaccharomyces pombe Puslp (Hellmuth et al., Nucl. Acids res. 28: 4604-4610); E.
  • coli RluD (Wrzesinski et al., 2000, IUBMB Life, 50: 33-37); and 23S RNA pseudouridine 2633 synthase from B. subtilis.
  • Queuosine can replace guanine in tRNA and is incorporated by the enzyme tRNA guanine glycosylase in both bacteria and eukaryotes.
  • Wyosine is made by the multi-step modification of an existing guanine or inosine, proceeding via Nl-methylguanosine [Droogmans & Grosjean, 1987] using a G37- Nl-methylguanosine-tRNA-methyltransferase.
  • the enzyme 2'-O-ribosyl phosphate transferase introduces a ribose at the 2' position of a guanine in eukaryotic tRNA (e.g. Ritl [Astrom & Bystrom, 1994]).
  • eukaryotic tRNA e.g. Ritl [Astrom & Bystrom, 1994]
  • a summary of modifications is given in Table I.
  • Acetylases are involved in N4-acetylcytidine, N4-acetyl-2'-O-methylcytidine modifications.
  • Thil and IscS are involved in 4-thiouridine biosynthesis.
  • Aminoacyl-tRNA-synthetases are not considered RNA modifying enzymes according to the invention.
  • RNA-modifying enzymes include, but are not limited to methyltransferases (e.g. thiostrepton resistance methyltransferase, erythromycin resistance methyltransferase, G37-Nl-methylguanosine-tRNA- methyltransferases), pseudouridine synthases (e.g. pseudouridine synthase I), guanine glycosylases, methylguanosine-tRNA-methyltransferases, 2'-O-ribosyl phosphate transferases (e.g. Ritl), acetylases, Thil and IscS.
  • methyltransferases e.g. thiostrepton resistance methyltransferase, erythromycin resistance methyltransferase, G37-Nl-methylguanosine-tRNA- methyltransferases
  • pseudouridine synthases e.g. pseudouridine synthase I
  • guanine glycosylases methylgu
  • RNA modifying enzymes can also be used, as can allelic variants and mutants.
  • Enzymes used in the invention are preferably in an essentially pure state, but the invention can also utilise impure enzyme or a cell extract.
  • RNA-modifying enzymes have very stringent structural or sequence requirements for the RNA substrate. Using conventional techniques of protein engineering, therefore, it is possible to modify a known enzyme to alter its substrate preference. In this way, an available enzyme can be tailored to modify a target RNA of interest, for use in the methods ofthe invention.
  • RNA modifying activity exploits sequence complementarity within the snoRNA as a guide to the complementary sequence on the target rRNA.
  • snoRNP small nucleolar ribonucleolar protein complex
  • RNA component consists of an RNA guide sequence that has sequence complementarity (10-21 nucleotides) to the target rRNA and two sequence motifs: box C at the 5' end of the snoRNA molecule and D at the 3' end, box D (or D') is positioned five base pairs from the methylated nucleotide.
  • rRNA modifying snoRNP family is associated with a pseudouridilation activity and is also involved in the maturation of eukaryotic pre-rRNA [Samarsky et al, 1998].
  • a pseudouridine synthase is responsible for the pseudouridilation activity.
  • the sno RNA component that acts as a guide sequence has a hairpin-hinge-hairpin-tail secondary structure and two essential sequence elements: the 5' hinge region (box H), has a consensus sequence ANANNA and the 3' tail region (box AC A) has a consensus sequence ACA but can include the sequences AUA and AAA.
  • Pseudouridine formation takes place in pre-rRNA at regions that are homologous to boxes ACA and box H but at positions within the pre-rRNA that are complementary to box H. Sequence changes within box H and box ACA that introduce sequence complementarity to a target RNA of interest, would make the target RNA accessible for use in the methods ofthe invention.
  • Suitable enzymes can also been identified by data-mining genomic sequences, based on the nucleotide sequence and structure characteristics of known enzymes [e.g. Klimasauskas et al, 1989; Schluckebier et al, 1995].
  • the methods of the invention involve the detection and/or measurement of target RNA modification by the RNA-modifying enzyme (i.e. an indirect measurement of the interaction of the test compound and the RNA target).
  • detecting the modification of target RNA refers to the process whereby one determines whether a particular RNA modifying enzyme has covalently modified the target RNA.
  • the detection and/or measurement of target RNA modification can be performed in a variety of ways, depending upon the nature ofthe modification (see, for example, Table I).
  • comparing the amount of modification detected to a standard refers to the process whereby the amount of modification by a given RNA modifying enzyme is determined in separate reactions performed with and without a test compound.
  • the modification detected in the reaction performed without the test compound is used as a standard for comparison with the amount detected in the presence of test compound.
  • RNA-modifying enzyme is a methyltransferase
  • the modification can interfere with reverse transcriptase primer extension of a cDNA, or with a specific endonuclease.
  • Radioactively labelled products of the primer extension reaction or end-labelled fragments of the target RNA can be analysed on a sequencing gel. The appearance or disappearance of a band, specific for a modification, indicates RNA modification. These can be quantified by scanning autoradiographs or phosphorimager analysis.
  • RNA can be precipitated by the addition of TCA, TFA or ethanol solution. Precipitated RNA can then be isolated by centrifugation or by retention on filters (e.g. glass fibre, nitrocellulose, or other suitable filters).
  • filters e.g. glass fibre, nitrocellulose, or other suitable filters.
  • the methods of the invention can involve the detection or measurement of the modification of the RNA-modifying enzyme by the incorporation of a non-competitive, irreversible, suicide substrate from the target RNA.
  • suicide substrate refers to an enzyme substrate, e.g., a target RNA or a nucleotide or base within the target RNA, that when modified by the enzyme, irreversibly binds to and inhibits the further activity of the enzyme. Under these circumstances, one detects the activity of the enzyme on the target RNA by detecting a label incorporated into the target RNA that becomes bound to the enzyme.
  • RNA-modifying enzyme is a pseudouridine synthase
  • measurement of modification can be based around the incorporation of an isotopic label from 5-F uridine (e.g. ⁇ P-FUTP), a suicide substrate incorporated into the target RNA [Huang et al, 1998].
  • modified protein any standard protein extraction protocol can be used e.g. protein can be isolated by retention on filters.
  • Test compound any standard protein extraction protocol can be used e.g. protein can be isolated by retention on filters.
  • the present invention can be used to identify compounds capable of binding to any target RNA, preferably as part of a screening process.
  • test compound refers to an agent comprising a compound, molecule, or complex, that is being tested for its ability to bind to a target RNA.
  • Test compounds can be any agent, including, but not restricted to, peptides, peptoids, proteins, lipids, metals, nucleotides, nucleosides, small organic molecules, polyamines, and combinations and derivatives thereof. Small organic molecules have a molecular weight between 50 and about 2,500 daltons, and most preferably in the range 200-800 daltons.
  • Complex mixtures of substances such as extracts containing natural products, or the products of mixed combinatorial syntheses, can also be tested and the component that binds to the target RNA can be purified from the mixture in a subsequent step.
  • Test compounds can be derived or selected from large libraries of synthetic or natural compounds.
  • synthetic compound libraries are commercially available from Maybridge Chemical Co. (Trevillet, Cornwall, UK) or Aldrich (Milwaukee, WI).
  • libraries of natural compounds in the form of bacterial, fungal, plant and animal extracts may be used.
  • test compounds can be synthetically produced using combinatorial chemistry either as individual compounds or as mixtures. A collection of compounds made using combinatorial chemistry is referred to herein as a combinatorial library.
  • a compound is said to have antibiotic activity if it slows the growth of a microorganism (doubling time at least twice as long as doubling time in the absence of the compound), halts the growth of a microorganism, or kills a microorganism.
  • a test compound identified as an a candidate antibiotic using the methods of the invention can have antibiotic activity on its own, or it can be used in conjunction with another known antibiotic. While not wishing to be bound by any specific mechanism, a compound identified as a candidate antibiotic can function directly as an antibiotic by binding to an RNA and interfering with its function (e.g., binding to bacterial rRNA, preventing translation).
  • the candidate antibiotic can bind to an RNA, preventing its modification by an enzyme that confers resistance to another antibiotic, and thereby rendering the microorganism sensitive to that other antibiotic.
  • An example of this alternative mechanism is the binding of the compound to bacterial rRNA, which prevents the methylation of the rRNA, thereby rendering the bacterium sensitive to an antibiotic to which it was previously resistant.
  • the methods ofthe invention involve a comparison ofthe RNA modifying enzyme activity with a standard.
  • the standard is a control value measured in the absence of the test compound and is a property ofthe enzyme that can be used to measure its function.
  • the standard may have been determined before performing the method, or can be determined during or after the method has been performed. It can be an absolute standard.
  • target RNA and test compound are mixed in a first step.
  • Modification is started by adding the enzyme and its second substrate (e.g. SAM in the case of SAM-dependent methyltransferases). After incubation for a defined time, the enzymatic reaction is stopped (e.g. by precipitation of the RNA). The degree of modification is compared with a standard. A reduction in modification compared with the standard indicates that the test compound and RNA form a strong complex.
  • the term “reduction in modification” or “inhibition of modification” means that the amount of modification ofthe target RNA (or, when a suicide substrate is used, the enzyme itself) is at least 10% lower, and preferably 20% lower, 30% lower, 40% lower, 50% lower, 60% lower, 70% lower, 80% lower, 90% lower, 95% lower, 97% lower, 99% lower, or even up to and including 100%) lower (i.e., no modification) in the presence of a test compound than in the absence of that test compound.
  • the mixture can also include a known antibiotic, in order to measure the ability of a test compound to displace a bound antibiotic.
  • a known antibiotic in order to measure the ability of a test compound to displace a bound antibiotic.
  • Compounds that displace antibiotics but do not inhibit RNA modification will lead to an increase in modification compared with the standard.
  • test compounds can be enzyme inhibitors without also being able to bind to the target RNA. These would be identified as false positives in an assay where simple inhibition of modification is measured. In order to avoid these false positives, the test compounds are preferably also measured against a range of enzymes (e.g. where the enzyme is a methyltransferase, against O-catechol methyltransferases, or restriction enzyme methyltransferases) in order to distinguish general enzyme inhibition from the inhibition of specific target RNA modification. As an alternative control, test compounds can be tested for their ability to displace pre-bound antibiotics.
  • the reaction mixture can contain competitor RNA and DNA to increase the stringency of binding conditions and specificity of inhibition.
  • test compound competes for RNA binding with the enzyme. This provides specificity in the assay and permits exclusion of compounds that bind to the target RNA but do not interfere with RNA modifying enzymes.
  • K m for each titration is determined by fitting the data to the equation
  • the Kj value for a compound is determined by relating the K m in the presence and the K n in the absence of compound using the equation:
  • Ki [I]/((K m (I)/K ra )-l)
  • enzyme binding and activity is measured by measuring the Michealis constant (K m ) of the enzymatic reaction in the absence and presence of a constant concentration of the test compound. Increasing amounts of target RNA are incubated with the modifying enzyme and the yield of modified RNA product is measured for each point. Data from this substrate titration are fitted to a saturation curve by nonlinear regression. The Michaelis constant K m is determined from the curve-fitting by routine methods.
  • K m Michealis constant
  • modification is quantitated at a constant target RNA concentration and a constant concentration of test compound. Measurements can be performed in duplicate or multiple and compared to a standard without compound (single point measurement).
  • the method can be used in the identification of compounds that bind to the target RNA from within a plurality of test compounds, such as in screening methods.
  • the method can therefore involve the initial step of providing a plurality of test compounds, which can include compounds not already known to bind to the target RNA.
  • high throughput screening format refers to a screening format with the capacity to test large numbers of compounds efficiently, typically implying that a number of compounds are tested simultaneously. For example, using 96-well microtiter plates, up to 96 compounds can be tested at a time. In addition, the capacity can be further increased by using more than one 96 well plate (e.g., 2, 5, 10, 50, 100, 200 or more plates).
  • a high throughput screening format also has the capacity to simultaneously test a range of different concentrations of one or more test compounds.
  • all the biochemical steps for this assay are performed in a single solution in, for instance, a test tube or microtitre plate, and the test compounds are analysed initially at a single compound concentration.
  • the experimental conditions are adjusted to achieve a proportion of test compounds identified as "positive" compounds from amongst the total compounds screened.
  • the assay is preferably set to identify compounds with an appreciable affinity towards the target RNA eg. when 0.1% to 1% of the total test compounds from a large compound library are shown to bind to a given target RNA with a K,of lO ⁇ M or less (eg. l ⁇ M, lOOnM, lOnM, or less).
  • the invention also provides a kit for determining whether a test compound binds to a target RNA, the kit comprising the target RNA and a RNA-modifying activity.
  • Example 1 23S GAR as a methyltransferase substrate Streptomyces azureus produces a methyltransferase that introduces a single methyl group at the 2'0 position of adenosine-1067 in the GTPase activating region (GAR) of its 23 S rRNA ( Figure 2).
  • the GAR is the site of action of the thiazole antibiotic thiostrepton, and the gene encoding the methyltransferase (tsr) confers resistance in Streptomyces azureus.
  • Thiostrepton has been shown to bind a 60mer fragment ('mGAR' herein) ofthe GAR.
  • the tsr gene (John Innes Foundation, Norwich) was cloned and overexpressed in E.coli.
  • the methylation activity of the tsr methyltransferase was investigated using complete 23 S E.coli rRNA and the 60mer mGAR from Thermotoga maritima. (sequence:
  • RNA (0-2 ⁇ M) was incubated in 25mM HEPES-KOH, pH 7.5, 25mM NFLG1, 5mM MgCl 2 , 5mM DTT with test compounds.
  • the methylation reaction was started by adding 8pmol TSR methyltransferase and l ⁇ Ci [ 3 H]SAM (Amersham-Pharmacia). The final assay volume was lOO ⁇ l.
  • the reaction was incubated 15 min at 25°C and stopped by adding 1 vol. 2% TFA.
  • the TFA precipitate was filtered through a 96-well glassfiber filterplate (Millipore Multiscreen FB l.O ⁇ M Glass Fiber Type B Filter). The filters were washed with 2 vol. of 2% TFA.
  • RNA substrates Four further RNA substrates were tested: the left-hand loop (LHL) 29mer of E.coli mGAR (GGAUGUUGGCUUAGAAGCAGCCAUCAUCC, methylation site underlined); a 17mer fragment of that 29mer (GGGCUUAGAAGCAGCCU, methylation site underlined); and Homo sapiens mGAR (GGCAGGACGGUGGCCAUGGAAGUCGGAAUCCGCUAAGGAGUGUGUAACAACUCACCUGCC, the underlined residue being the homologous position to the methylation site in prokaryotes).
  • LHL left-hand loop
  • GGAUGUUGGCUUAGAAGCAGCCAUCAUCC methylation site underlined
  • Homo sapiens mGAR GGCAGGACGGUGGCCAUGGAAGUCGGAAUCCGCUAAGGAGUGUGUAACAACUCACCUGCC, the underlined residue being the homologous position to the methylation site in prokaryotes.
  • LHL left-hand loop
  • the E.coli mGAR and the LHL fragment were tested as above at a variety of concentrations, and the binding curve is shown in figure 4B.
  • the E.coli mGAR was a good substrate, with a K ra of 50nM, compared with 2 ⁇ M for LHL. This suggests that the RNA needs to be at least partially folded to be recognised by the tsr enzyme.
  • the Lll ribosomal protein is an integral part of the GAR, and its presence inhibits methyltransferase activity [Bechthold & Floss, 1994].
  • Various concentrations of Ll l were incubated with E.coli GAR and [ 3 H]SAM as described above. As expected, methylation was inhibited as LI 1 concentration increased.
  • the affinity of the interaction (84nM; figure 5) is comparable to published values.
  • thiostrepton does not bind to 23 S which is methylated at A- 1067. Methyltransferase and thiostrepton thus compete for binding at A- 1067, and binding of thiostrepton to 23 S (or GAR) can be measured by following inhibition of methylation.
  • Figure 6 shows the result of this experiment, which gave an affinity of 1.1 ⁇ M, comparable to published values.
  • the tsr methyltransferase product is thus an intimate probe of intermolecular contacts in the GAR, and can measure interactions specifically at positions where antibiotics interact. Compounds which inhibit the methylation (and thus inhibit antibiotic resistance) can therefore be identified using this assay.
  • Test compounds were assayed in a 96 well plate for inhibition of tsr methylation activity at 50 ⁇ M. Compounds were incubated at 25°C for 30 minutes in the presence of [ 3 H]SAM and enzyme, and increasing amounts of mGAR were added. RNA was precipitated with 2% TFA, filtered, and scintillation counted in a Wallac Trilux instrument.
  • Figure 7 shows the results of this experiment using neomycin, RBT-A, RBT-B and RBT-C as test compounds. It is clear that all compounds show significant inhibition of methyltransferase, consistent with the K; values shown in the figure. RBT-C shows very good inhibition of methylation
  • Methyltransferase activity can be recovered by deletion of the N-terminal domain of Lll (figure 15). It is therefore possible to modify the assay such that fragments of proteins are incorporated.
  • the concentration of Ll l and Lll-C-domain was l ⁇ M.
  • the assay was performed in 25mM HEPES/KOH, pH 7.5, 5mM MgCl 2 , 25mM NH 4 C1, 5mM DTT.
  • GST-TSR methylase (lOOnM final cone.) and 0.05 ⁇ Ci of [ 3 H]SAM (Amersham Pharmacia Biotech) were added in a final volume of lOO ⁇ l.
  • GAR domain RNA transcript was titrated from 0- 320nM.
  • the assay was incubated for 30 min at 25°C in wells of a Multiscreen 96-well glass fiber filter plate (Millipore). The assay was stopped by precipitating with 1 vol.
  • rRNA ribosomal RNA
  • the Methyltransferase activity of the TSR methyltransferase assay is highly consistent, in experiments where either ribosomal RNA or GAR 58mer RNA are methylated, the K ra can be measured reproducibly. Data obtained were fitted to the Michaelis-Menten equation by non-linear least-square fitting using GraphPad Prism software. Error bars represent the standard error of means, the K m R and number of experiments are indicated ( Figure 16). The K m measured for rRNA over 20 experiments is 27 (+/- 5) nM. The K m for GAR 58mer RNA over 15 experiments is 47(+/-3) nM (figure 16).
  • Figure 16 shows data from fifteen independent methylation reactions of Gar 58mer are presented as titrations.
  • Figure 16B shows data from twenty independent methylation reactions of rRNA are presented as titrations.
  • the assay can be implemented in a single point format.
  • the consistency and reproducibility of this assay format is shown in figure 17.
  • the same set of 80 compounds were assayed in single points at 50 ⁇ M.
  • the activities of the compounds in the two assays were plotted against each other. Active compounds (those that show greater than 25% inhibition) lie close to the diagonal, demonstrating the reproducibility of the single point assay format. Activities are given as % inhibition compared to the control in the absence of compound.
  • the erythromycin resistance methyltransferases are a class of methyltransferases (Erm) that confer resistance to the macrolide class of antibiotics, exemplified by erythromycin, modification occurs in the peptidyl transferase centre of 23S rRNA (figure 8). ErmE incorporates methyl groups into 23 S rRNA ( Figure 18) with a Km of 23nM.
  • the reaction was performed in lOO ⁇ l 20mM HEPES/KOH, pH 7.8, lOmM MgCl 2 , lOOmM NH4C1, Im DTT, 10% glycerol for 30min at 25°C with O.Ol ⁇ M GST-Erm methylase and 0.5 ⁇ Ci [3H]SAM.
  • the Km derived from the data shown is 23nM.
  • Assays were performed in 96-well format in 25mM HEPES/KOH, pH 7.5, 5mM MgCl 2 , 25mM NH 4 C1, 5mM DTT.
  • ErmE methylase (lOOnM final cone.) and 0.05 ⁇ Ci of [ 3 H]SAM (Amersham Pharmacia Biotech) were added in a final volume of lOO ⁇ l.
  • GAR domain RNA transcript was titrated from 0-320nM.
  • the assay was incubated for 30 min at 25°C in wells of a Multiscreen 96-well glass fiber filter plate (Millipore). The assay was stopped by precipitating with 1 vol. of 2%TFA and washing twice with 2 vol. of 2%TFA. After drying of the filters, 40 ⁇ l of Optiphase Supermix scintillation liquid (PerkinElmer) were added per well and the incorporated radioactivity was counted in a Trilux scintillation counter (PerkinElmer).
  • the methylation activity of the methytransferase is dependant on the conformation of the RNA ( Figure 19), and significantly less radioactivity is incorporated into rRNA that has undergone a folding/hybridisation step.
  • Figure 19 shows the effect of refolding on rRNA methylation by GST-Erm.
  • the reaction was performed in 50 ⁇ l 50mM Tris/Cl, pH 7.5, 4mM MgCl 2 , 40mM KC1, lOmM DTT, for 120min at 37°C with 0.04 ⁇ M rRNA and 6 ⁇ Ci [3H]SAM.
  • the rRNA as either untreated or folded by heating 3min at 65°C and then stored on ice.
  • the incorporation of radioactivity into ribosomal RNA was measured over a range of magnesium concentrations (figure 20).
  • the stringency of the assay may be altered by adjusting the magnesium concentration in the assay.
  • the assay was performed in 50mM Tris/Cl, pH 7.5, 40mM KC1, lOmM DTT.
  • rRNA (Roche) was incubated 3 min at 65°C, cooled down to room temperature over 3 h, then put on ice and stored at - 20°C.
  • Example 7 Inhibition of erythromycin resistance methylase ermE by erythromycin.
  • Erm E is inhibited by erythromycin (figure 21). Erythromycin inhibits enzyme activity most noticably when the RNA is folded in the presence of erythromycin (figure 21).
  • One embodiment of the invention may therefore involve folding of the RNA in the presence of compound before measuring the activity ofthe methyltransferase on the RNA.
  • Figure 21 shows the decrease in refolded rRNA methylation by Erm E in the presence of erythromyin.
  • the reaction was performed in 50 ⁇ l 50mM Tris/Cl, pH 7.5, 4mM MgCl 2 , 40mM KC1, lOmM DTT, for 120min at 37°C with 0.04 ⁇ M rRNA and 6 ⁇ Ci [3H]SA .
  • the erythromycin concentration was 200 ⁇ M. Erythromycin showed no effect when used with unfolded rRNA.
  • Example 8 Inhibition of erythromycin resistance methylase ermE by test compounds in a single point assay format
  • a series of compounds A-J were assayed at 50 ⁇ M, compounds C-G and J showed significant (greater than 40%) inhibition of the methyltransferase.
  • the ermE assay can therefore be implemented in a high throughput screening format.
  • Figure 22 shows inhibition of rRNA methylation with ERM by various compounds.
  • the identity is unimportant as the present example merely serves to demonstrate the utility of a single point assay format.
  • the assay was performed as above, except that the MgCl concentration was lOmM and the final rRNA concentration was 40nM.
  • Compounds A-J were assayed at 50 ⁇ M.
  • the final DMSO concentration was 1%. The activity is given relative to the average activity in the absence of compound.
  • CMC l-cyclohexyl-3-(2-mo ⁇ holinoethyl) carbodiimide metho-p-toluene sulfonate
  • Ribosomal RNA is the target for oxazolidinones, a novel class of translational inhibitors. Rna 5, 939-46.
  • C/D motif directs nucleolar targeting and also couples snoRNA synthesis and localization. Embo J 17, 3747-57.
  • Nucleotide sequence ofthe ksgA gene of Escherichia coli comparison of methyltransferases effecting dimethylation of adenosine in ribosomal RNA. Gene 38, 65-72. von Ahsen, U., Davies, J., and Schroeder, R. (1991). Antibiotic inhibition of group I ribozyme function [see comments]. Nature 353, 368-70.

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Abstract

L'invention concerne une méthode permettant de déterminer si un composé d'essai peut se fixer à un ARN cible, la méthode consistant (a) à mettre en contact le composé d'essai avec l'ARN cible et une enzyme modificatrice d'ARN; et (b) à détecter la modification de l'ARN cible causée par l'enzyme et à comparer le niveau de modification détecté à un niveau standard. L'inhibition de la modification enzymatique de l'ARN cible indique que le composé d'essai peut se fixer à l'ARN cible et peut ainsi présenter une activité antibiotique.
EP01969049A 2000-04-19 2001-04-19 Analyse d'identification d'un compose d'essai Withdrawn EP1343910A2 (fr)

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