EP1399538A4 - DEVELOPMENT OF INTRACELLULAR SIALYLATION PATHWAYS - Google Patents

DEVELOPMENT OF INTRACELLULAR SIALYLATION PATHWAYS

Info

Publication number
EP1399538A4
EP1399538A4 EP00913684A EP00913684A EP1399538A4 EP 1399538 A4 EP1399538 A4 EP 1399538A4 EP 00913684 A EP00913684 A EP 00913684A EP 00913684 A EP00913684 A EP 00913684A EP 1399538 A4 EP1399538 A4 EP 1399538A4
Authority
EP
European Patent Office
Prior art keywords
methods
interest
cells
carbohydrate
pathways
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP00913684A
Other languages
German (de)
French (fr)
Other versions
EP1399538A2 (en
Inventor
Michael J Betenbaugh
Shawn Lawrence
Yuan C Lee
Don Jarvis
Timothy A Coleman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Human Genome Sciences Inc
Johns Hopkins University
University of Wyoming
Original Assignee
Human Genome Sciences Inc
Johns Hopkins University
University of Wyoming
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Human Genome Sciences Inc, Johns Hopkins University, University of Wyoming filed Critical Human Genome Sciences Inc
Publication of EP1399538A2 publication Critical patent/EP1399538A2/en
Publication of EP1399538A4 publication Critical patent/EP1399538A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01052—Beta-N-acetylhexosaminidase (3.2.1.52)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1081—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/026—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a baculovirus

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Methods for manipulating carbohydrate processing pathways in cells of interest are provided. Methods are directed at manipulating multiple pathways involved with the sialylation reaction by using recombinant DNA technology and substrate feeding approaches to enable the production of sialylated glycoproteins in cells of interest. These carbohydrate engineering efforts encompass the implementation of new carbohydrate bioassays, the examination of a selection of insect cell lines and the use of bioinformatics to identify gene sequences for critical processing enzymes. The compositions comprise cells of interest producing sialylated glycoproteins. The methods and compositions are useful for heterologous expression of glycoproteins.
EP00913684A 1999-03-02 2000-03-01 DEVELOPMENT OF INTRACELLULAR SIALYLATION PATHWAYS Withdrawn EP1399538A4 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US12258299P 1999-03-02 1999-03-02
US122582P 1999-03-02
US16962499P 1999-12-08 1999-12-08
US169624P 1999-12-08
PCT/US2000/005313 WO2000052135A2 (en) 1999-03-02 2000-03-01 Engineering intracellular sialylation pathways

Publications (2)

Publication Number Publication Date
EP1399538A2 EP1399538A2 (en) 2004-03-24
EP1399538A4 true EP1399538A4 (en) 2004-03-24

Family

ID=26820692

Family Applications (1)

Application Number Title Priority Date Filing Date
EP00913684A Withdrawn EP1399538A4 (en) 1999-03-02 2000-03-01 DEVELOPMENT OF INTRACELLULAR SIALYLATION PATHWAYS

Country Status (5)

Country Link
EP (1) EP1399538A4 (en)
JP (1) JP2003524395A (en)
AU (1) AU3508300A (en)
CA (1) CA2363297C (en)
WO (1) WO2000052135A2 (en)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6949372B2 (en) 1999-03-02 2005-09-27 The Johns Hopkins University Engineering intracellular sialylation pathways
AU2001236739A1 (en) * 2000-02-08 2001-08-20 Genentech Inc. Improved sialylation of glycoproteins
US7863020B2 (en) * 2000-06-28 2011-01-04 Glycofi, Inc. Production of sialylated N-glycans in lower eukaryotes
KR101495616B1 (en) 2002-03-07 2015-02-26 아이드게노쉬쉐 테흐니쉐 호흐슐레 쥬리히 System and method for the production of recombinant glycosylated protein in a prokaryotic host
IL163806A0 (en) * 2002-03-07 2005-12-18 Eidgenoess Tech Hochschule System and method for the production of recombinant glycosylated proteins in a prokaryotic host
JP2006333701A (en) * 2003-01-15 2006-12-14 Kazuhito Fujiyama Production method of glycoprotein having animal type sugar chain
CN103627670B (en) * 2004-02-13 2016-12-21 格莱克托普有限公司 High activity glycoprotein processing conditions and effectively production method
EP1730293A2 (en) * 2004-03-17 2006-12-13 Glycofi, Inc. Method of engineering a cytidine monophosphate-sialic acid synthetic pathway in fungi and yeast
US20090298120A1 (en) * 2004-05-04 2009-12-03 National University Of Singapore Method for expressing sialylated glycoproteins in mammalian cells and cells thereof
US7479549B2 (en) 2005-02-23 2009-01-20 Jaques John Scott T Recombinant canine thyroid stimulating hormone and methods of production and use thereof
ES2353814T3 (en) 2005-05-11 2011-03-07 Eth Zuerich RECOMBINANT N-GLYCOSILATED PROTEINS OF PROCEDURAL CELLS.
DE602006015195D1 (en) * 2005-07-12 2010-08-12 Greenovation Biotech Gmbh IMPROVEMENTS OF PROTEIN PRODUCTION OR IN CONNECTION WITH THIS
EP3539981A1 (en) 2006-09-10 2019-09-18 Glycotope GmbH Use of human cells of myeloid leukaemia origin for expression of antibodies
PL1920781T3 (en) 2006-11-10 2015-06-30 Glycotope Gmbh Compositions comprising a core-1 positive microorganism and their use for the treatment or prophylaxis of tumors
CN101983070B (en) 2008-02-20 2016-03-30 格林考瓦因有限公司 The recombinant N-glycosylated proteins that origin comes from prokaryotic cell prepares bioconjugates
NO2501406T3 (en) 2009-11-19 2018-05-19
EP3281639B1 (en) 2010-05-06 2020-11-11 GlaxoSmithKline Biologicals S.A. Capsular gram-positive bacteria bioconjugate vaccines
EP2412815A1 (en) * 2010-07-27 2012-02-01 Universite De Rouen N-glycosylation in transformed Phaeodactylum tricornutum
WO2012050175A1 (en) * 2010-10-15 2012-04-19 日本ケミカルリサーチ株式会社 Method for producing glycoprotein having mannose residue as non-reducing end of sugar chain
ES2686313T3 (en) 2011-08-22 2018-10-17 Glycotope Gmbh Microorganisms that carry a tumor antigen
JP6652334B2 (en) † 2014-05-31 2020-02-19 Ｊｃｒファーマ株式会社 Medium containing uridine and N-acetyl-D-mannosamine
SG11202010496WA (en) 2018-05-18 2020-12-30 Daiichi Sankyo Co Ltd Anti-muc1 antibody-drug conjugate

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
GU XUEJUN ET AL: "Improvement of interferon-gamma sialylation in Chinese hamster ovary cell culture by feeding of N-acetylmannosamine", BIOTECHNOLOGY AND BIOENGINEERING, INTERSCIENCE PUBLISHERS, LONDON, GB, vol. 58, no. 6, 20 June 1998 (1998-06-20), pages 642 - 648, XP002171407, ISSN: 0006-3592 *
OGONAH O W ET AL: "ISOLATION AND CHARACTERIZATION OF AN INSECT CELL LINE ABLE TO PERFORM COMPLEX N-LINKED GLYCOSYLATION ON RECOMBINANT PROTEINS", BIOTECHNOLOGY, BUTTERWORTHS, LONDON, GB, vol. 14, February 1996 (1996-02-01), pages 197 - 202, XP000961423, ISSN: 0740-7378 *
See also references of WO0052135A3 *

Also Published As

Publication number Publication date
WO2000052135A3 (en) 2004-01-08
CA2363297A1 (en) 2000-09-08
AU3508300A (en) 2000-09-21
EP1399538A2 (en) 2004-03-24
WO2000052135A9 (en) 2001-10-11
WO2000052135A2 (en) 2000-09-08
JP2003524395A (en) 2003-08-19
CA2363297C (en) 2011-08-09

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