WO2000052135A3 - Engineering intracellular sialylation pathways - Google Patents

Engineering intracellular sialylation pathways Download PDF

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Publication number
WO2000052135A3
WO2000052135A3 PCT/US2000/005313 US0005313W WO0052135A3 WO 2000052135 A3 WO2000052135 A3 WO 2000052135A3 US 0005313 W US0005313 W US 0005313W WO 0052135 A3 WO0052135 A3 WO 0052135A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
interest
cells
carbohydrate
pathways
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2000/005313
Other languages
French (fr)
Other versions
WO2000052135A9 (en
WO2000052135A2 (en
Inventor
Michael J Betenbaugh
Shawn Lawrence
Yuan C Lee
Don Jarvis
Timothy A Coleman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Human Genome Sciences Inc
Johns Hopkins University
University of Wyoming
Original Assignee
Human Genome Sciences Inc
Johns Hopkins University
University of Wyoming
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Human Genome Sciences Inc, Johns Hopkins University, University of Wyoming filed Critical Human Genome Sciences Inc
Priority to EP00913684A priority Critical patent/EP1399538A4/en
Priority to AU35083/00A priority patent/AU3508300A/en
Priority to JP2000602747A priority patent/JP2003524395A/en
Priority to CA2363297A priority patent/CA2363297C/en
Publication of WO2000052135A2 publication Critical patent/WO2000052135A2/en
Anticipated expiration legal-status Critical
Publication of WO2000052135A9 publication Critical patent/WO2000052135A9/en
Publication of WO2000052135A3 publication Critical patent/WO2000052135A3/en
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01052—Beta-N-acetylhexosaminidase (3.2.1.52)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1081—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/026—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a baculovirus

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Methods for manipulating carbohydrate processing pathways in cells of interest are provided. Methods are directed at manipulating multiple pathways involved with the sialylation reaction by using recombinant DNA technology and substrate feeding approaches to enable the production of sialylated glycoproteins in cells of interest. These carbohydrate engineering efforts encompass the implementation of new carbohydrate bioassays, the examination of a selection of insect cell lines and the use of bioinformatics to identify gene sequences for critical processing enzymes. The compositions comprise cells of interest producing sialylated glycoproteins. The methods and compositions are useful for heterologous expression of glycoproteins.
PCT/US2000/005313 1999-03-02 2000-03-01 Engineering intracellular sialylation pathways Ceased WO2000052135A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP00913684A EP1399538A4 (en) 1999-03-02 2000-03-01 DEVELOPMENT OF INTRACELLULAR SIALYLATION PATHWAYS
AU35083/00A AU3508300A (en) 1999-03-02 2000-03-01 Engineering intracellular sialylation pathways
JP2000602747A JP2003524395A (en) 1999-03-02 2000-03-01 Manipulation of the intracellular sialylation pathway
CA2363297A CA2363297C (en) 1999-03-02 2000-03-01 Engineering intracellular sialylation pathways

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US12258299P 1999-03-02 1999-03-02
US60/122,582 1999-03-02
US16962499P 1999-12-08 1999-12-08
US60/169,624 1999-12-08

Publications (3)

Publication Number Publication Date
WO2000052135A2 WO2000052135A2 (en) 2000-09-08
WO2000052135A9 WO2000052135A9 (en) 2001-10-11
WO2000052135A3 true WO2000052135A3 (en) 2004-01-08

Family

ID=26820692

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/005313 Ceased WO2000052135A2 (en) 1999-03-02 2000-03-01 Engineering intracellular sialylation pathways

Country Status (5)

Country Link
EP (1) EP1399538A4 (en)
JP (1) JP2003524395A (en)
AU (1) AU3508300A (en)
CA (1) CA2363297C (en)
WO (1) WO2000052135A2 (en)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6949372B2 (en) 1999-03-02 2005-09-27 The Johns Hopkins University Engineering intracellular sialylation pathways
AU2001236739A1 (en) * 2000-02-08 2001-08-20 Genentech Inc. Improved sialylation of glycoproteins
US7863020B2 (en) * 2000-06-28 2011-01-04 Glycofi, Inc. Production of sialylated N-glycans in lower eukaryotes
KR101495616B1 (en) 2002-03-07 2015-02-26 아이드게노쉬쉐 테흐니쉐 호흐슐레 쥬리히 System and method for the production of recombinant glycosylated protein in a prokaryotic host
IL163806A0 (en) * 2002-03-07 2005-12-18 Eidgenoess Tech Hochschule System and method for the production of recombinant glycosylated proteins in a prokaryotic host
JP2006333701A (en) * 2003-01-15 2006-12-14 Kazuhito Fujiyama Production method of glycoprotein having animal type sugar chain
CN103627670B (en) * 2004-02-13 2016-12-21 格莱克托普有限公司 High activity glycoprotein processing conditions and effectively production method
EP1730293A2 (en) * 2004-03-17 2006-12-13 Glycofi, Inc. Method of engineering a cytidine monophosphate-sialic acid synthetic pathway in fungi and yeast
US20090298120A1 (en) * 2004-05-04 2009-12-03 National University Of Singapore Method for expressing sialylated glycoproteins in mammalian cells and cells thereof
US7479549B2 (en) 2005-02-23 2009-01-20 Jaques John Scott T Recombinant canine thyroid stimulating hormone and methods of production and use thereof
ES2353814T3 (en) 2005-05-11 2011-03-07 Eth Zuerich RECOMBINANT N-GLYCOSILATED PROTEINS OF PROCEDURAL CELLS.
DE602006015195D1 (en) * 2005-07-12 2010-08-12 Greenovation Biotech Gmbh IMPROVEMENTS OF PROTEIN PRODUCTION OR IN CONNECTION WITH THIS
EP3539981A1 (en) 2006-09-10 2019-09-18 Glycotope GmbH Use of human cells of myeloid leukaemia origin for expression of antibodies
PL1920781T3 (en) 2006-11-10 2015-06-30 Glycotope Gmbh Compositions comprising a core-1 positive microorganism and their use for the treatment or prophylaxis of tumors
CN101983070B (en) 2008-02-20 2016-03-30 格林考瓦因有限公司 The recombinant N-glycosylated proteins that origin comes from prokaryotic cell prepares bioconjugates
NO2501406T3 (en) 2009-11-19 2018-05-19
EP3281639B1 (en) 2010-05-06 2020-11-11 GlaxoSmithKline Biologicals S.A. Capsular gram-positive bacteria bioconjugate vaccines
EP2412815A1 (en) * 2010-07-27 2012-02-01 Universite De Rouen N-glycosylation in transformed Phaeodactylum tricornutum
WO2012050175A1 (en) * 2010-10-15 2012-04-19 日本ケミカルリサーチ株式会社 Method for producing glycoprotein having mannose residue as non-reducing end of sugar chain
ES2686313T3 (en) 2011-08-22 2018-10-17 Glycotope Gmbh Microorganisms that carry a tumor antigen
JP6652334B2 (en) † 2014-05-31 2020-02-19 Ｊｃｒファーマ株式会社 Medium containing uridine and N-acetyl-D-mannosamine
SG11202010496WA (en) 2018-05-18 2020-12-30 Daiichi Sankyo Co Ltd Anti-muc1 antibody-drug conjugate

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
MUNSTER. ET. AL.: "Mammalian cytidine 5'-monophosphate N-acetylneuraminic acid synthetase: A nuclear protein with avolutionarly conserved structural motifs.", PROC. NATL. ACAD. SCI. USA, vol. 95, August 1998 (1998-08-01), pages 9140 - 9145, XP002928328 *
See also references of EP1399538A4 *

Also Published As

Publication number Publication date
CA2363297A1 (en) 2000-09-08
AU3508300A (en) 2000-09-21
EP1399538A2 (en) 2004-03-24
WO2000052135A9 (en) 2001-10-11
EP1399538A4 (en) 2004-03-24
WO2000052135A2 (en) 2000-09-08
JP2003524395A (en) 2003-08-19
CA2363297C (en) 2011-08-09

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