EP1501853A4 - Inhibition mediee par interference arn de l'expression genique d'un recepteur de facteur de croissance epidermique faisant appel a des acides nucleiques interferants courts - Google Patents

Inhibition mediee par interference arn de l'expression genique d'un recepteur de facteur de croissance epidermique faisant appel a des acides nucleiques interferants courts

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Publication number
EP1501853A4
EP1501853A4 EP03716093A EP03716093A EP1501853A4 EP 1501853 A4 EP1501853 A4 EP 1501853A4 EP 03716093 A EP03716093 A EP 03716093A EP 03716093 A EP03716093 A EP 03716093A EP 1501853 A4 EP1501853 A4 EP 1501853A4
Authority
EP
European Patent Office
Prior art keywords
nucleic acid
short interfering
rna
sina
rna interference
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP03716093A
Other languages
German (de)
English (en)
Other versions
EP1501853A2 (fr
Inventor
James Mcswiggen
Pamela Pavco
Leonid Beigelman
Kathy Fosnaugh
Sharon Jamison
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sirna Therapeutics Inc
Original Assignee
Sirna Therapeutics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2002/016840 external-priority patent/WO2002097114A2/fr
Priority claimed from US10/251,117 external-priority patent/US20030170891A1/en
Priority claimed from US10/277,494 external-priority patent/US20030186909A1/en
Application filed by Sirna Therapeutics Inc filed Critical Sirna Therapeutics Inc
Priority claimed from PCT/US2003/005045 external-priority patent/WO2003070912A2/fr
Publication of EP1501853A2 publication Critical patent/EP1501853A2/fr
Publication of EP1501853A4 publication Critical patent/EP1501853A4/fr
Withdrawn legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1138—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against receptors or cell surface proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/31—Chemical structure of the backbone
    • C12N2310/315—Phosphorothioates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/31—Chemical structure of the backbone
    • C12N2310/317—Chemical structure of the backbone with an inverted bond, e.g. a cap structure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/31—Chemical structure of the backbone
    • C12N2310/318—Chemical structure of the backbone where the PO2 is completely replaced, e.g. MMI or formacetal
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/321—2'-O-R Modification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/33—Chemical structure of the base
    • C12N2310/332—Abasic residue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

L'invention concerne des procédés et des réactifs utiles au niveau de la modulation de l'expression génique EGFR dans une variété d'applications, y compris des applications pour une utilisation thérapeutique et diagnostique, et pour une validation de cible, ainsi que des applications de découverte génomique. En particulier, l'invention concerne de petites molécules d'acides nucléiques, notamment des acides nucléiques interférants courts (siNA), un ARN interférant court (siRNA), un ARN bicaténaire (dsRNA), un micro ARN (miRNA) et des molécules d'ARN court en épingle à cheveux (shRNA) permettant une médiation d'interférence ARN (RNAi) contre HER1, HER2, HER3 et/ou HER4. Ces petites molécules d'acides nucléiques sont utiles pour le diagnostic et pour le traitement du cancer.
EP03716093A 2002-02-20 2003-02-20 Inhibition mediee par interference arn de l'expression genique d'un recepteur de facteur de croissance epidermique faisant appel a des acides nucleiques interferants courts Withdrawn EP1501853A4 (fr)

Applications Claiming Priority (25)

Application Number Priority Date Filing Date Title
US277494 1981-06-26
US251117 1988-09-29
US35858002P 2002-02-20 2002-02-20
US358580P 2002-02-20
US36312402P 2002-03-11 2002-03-11
US363124P 2002-03-11
PCT/US2002/016840 WO2002097114A2 (fr) 2001-05-29 2002-05-29 Traitement a l'acide nucleique de maladies ou d'affections associees aux taux de ras, her2 et hiv
WOPCT/US02/16840 2002-05-29
US38678202P 2002-06-06 2002-06-06
US10/163,552 US20030105051A1 (en) 2001-05-29 2002-06-06 Nucleic acid treatment of diseases or conditions related to levels of HER2
US386782P 2002-06-06
US163552 2002-06-06
US39392402P 2002-07-03 2002-07-03
US393924P 2002-07-03
US40678402P 2002-08-29 2002-08-29
US406784P 2002-08-29
US40837802P 2002-09-05 2002-09-05
US408378P 2002-09-05
US40929302P 2002-09-09 2002-09-09
US409293P 2002-09-09
US10/251,117 US20030170891A1 (en) 2001-06-06 2002-09-19 RNA interference mediated inhibition of epidermal growth factor receptor gene expression using short interfering nucleic acid (siNA)
US10/277,494 US20030186909A1 (en) 1997-01-27 2002-10-21 Nucleic acid treatment of diseases or conditions related to levels of epidermal growth factor receptors
US44012903P 2003-01-15 2003-01-15
US440129P 2003-01-15
PCT/US2003/005045 WO2003070912A2 (fr) 2001-06-06 2003-02-20 Inhibition mediee par interference arn de l'expression genique d'un recepteur de facteur de croissance epidermique faisant appel a des acides nucleiques interferants courts

Publications (2)

Publication Number Publication Date
EP1501853A2 EP1501853A2 (fr) 2005-02-02
EP1501853A4 true EP1501853A4 (fr) 2005-11-16

Family

ID=56290383

Family Applications (1)

Application Number Title Priority Date Filing Date
EP03716093A Withdrawn EP1501853A4 (fr) 2002-02-20 2003-02-20 Inhibition mediee par interference arn de l'expression genique d'un recepteur de facteur de croissance epidermique faisant appel a des acides nucleiques interferants courts

Country Status (3)

Country Link
EP (1) EP1501853A4 (fr)
JP (1) JP2005517437A (fr)
AU (1) AU2003219818A1 (fr)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000044895A1 (fr) * 1999-01-30 2000-08-03 Roland Kreutzer Methode et medicament destines a inhiber l'expression d'un gene donne
WO2002044321A2 (fr) * 2000-12-01 2002-06-06 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Petites molecules d'arn mediant l'interference arn

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000044895A1 (fr) * 1999-01-30 2000-08-03 Roland Kreutzer Methode et medicament destines a inhiber l'expression d'un gene donne
WO2002044321A2 (fr) * 2000-12-01 2002-06-06 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Petites molecules d'arn mediant l'interference arn

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ELBASHIR S M ET AL: "Functional anatomy of siRNAs for mediating efficient RNAi in Drosophila melanogaster embryo lysate", EMBO JOURNAL, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 20, no. 23, 3 December 2001 (2001-12-03), pages 6877 - 6888, XP002225998, ISSN: 0261-4189 *
PARRISH S ET AL: "Functional anatomy of a dsRNA trigger: Differential requirement for the two trigger strands in RNA interference", MOLECULAR CELL, CELL PRESS, CAMBRIDGE, MA, US, vol. 6, no. 5, November 2000 (2000-11-01), pages 1077 - 1087, XP002226298, ISSN: 1097-2765 *

Also Published As

Publication number Publication date
AU2003219818A8 (en) 2003-09-09
EP1501853A2 (fr) 2005-02-02
JP2005517437A (ja) 2005-06-16
AU2003219818A1 (en) 2003-09-09

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