EP1901757A2 - Preparations a base de substances naturelles - Google Patents

Preparations a base de substances naturelles

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Publication number
EP1901757A2
EP1901757A2 EP06792472A EP06792472A EP1901757A2 EP 1901757 A2 EP1901757 A2 EP 1901757A2 EP 06792472 A EP06792472 A EP 06792472A EP 06792472 A EP06792472 A EP 06792472A EP 1901757 A2 EP1901757 A2 EP 1901757A2
Authority
EP
European Patent Office
Prior art keywords
extracts
biomass
ganoderma
preparation
composition according
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP06792472A
Other languages
German (de)
English (en)
Inventor
Wolf-Dieter Juelich
Ulrike Lindequist
Sabine Witt
Joern Kasbohm
Gerold Lukowski
Kristian Wende
Hans-Peter Welzel
Winfrid Ruehle
Frank Szrama
Martina Wurster
Sebastian Lippemeier
Joachim Schomburg
Christian Schultz
Peter Hartig
Ralf Hintze
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Patenthandel Portfoliofonds I & Co KG GmbH
Universitaet Greifswald
Original Assignee
Ernst Moritz Arndt Universitaet Greifswald
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE102005031363A external-priority patent/DE102005031363A1/de
Priority claimed from DE102005031364A external-priority patent/DE102005031364A1/de
Priority claimed from DE102005031362A external-priority patent/DE102005031362A1/de
Application filed by Ernst Moritz Arndt Universitaet Greifswald filed Critical Ernst Moritz Arndt Universitaet Greifswald
Publication of EP1901757A2 publication Critical patent/EP1901757A2/fr
Withdrawn legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/06—Fungi, e.g. yeasts
    • A61K36/07—Basidiomycota, e.g. Cryptococcus
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/015—Inorganic compounds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/16—Inorganic salts, minerals or trace elements
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K33/00—Medicinal preparations containing inorganic active ingredients
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/06—Fungi, e.g. yeasts
    • A61K36/07—Basidiomycota, e.g. Cryptococcus
    • A61K36/074—Ganoderma
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K45/00—Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
    • A61K45/06—Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9706—Algae
    • A61K8/9717—Rhodophycota or Rhodophyta [red algae], e.g. Porphyra
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9706—Algae
    • A61K8/9722—Chlorophycota or Chlorophyta [green algae], e.g. Chlorella
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9728—Fungi, e.g. yeasts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2200/00—Function of food ingredients
    • A23V2200/20—Ingredients acting on or related to the structure
    • A23V2200/25—Nanoparticles, nanostructures
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/52—Stabilizers
    • A61K2800/522—Antioxidants; Radical scavengers

Definitions

  • the invention relates to compositions of biomass and / or extracts of at least one terrestrial fungus, preferably a species of the genus Ganoderma on the one hand and at least one layered silicate, an iron-rich raw material, biomass, obtainable from microalgae, fungal biopolymer complexes on the other.
  • the compositions prevent premature cell aging and have an anti-aging effect.
  • Possible applications include food and feed supplements, plant tonics or drugs that are designed to protect against free radicals. They are also suitable as UV protection agents based on natural substances.
  • a water extract or a low alcohol extract of G. lucidum can be used as a cosmetic (WO 01/32192 Al).
  • An alcoholic extract of G. lucidum can be used in dermatoses to prevent inflammation (JP 1180889 A).
  • G. lucidum In order to enhance the antioxidant effect, it is possible to mix G. lucidum with grapefruit seed extracts (WO 02/11744 Al). It is also possible to enrich G. lucidum during cultivation with selenium (WO 01/05938 Al). Also known are mixtures with the botanical antioxidants green tea extract, anthocyanins from bilberry fruits, ginkgo, guarana root extract (PCT WO 2004/100896 A2).
  • Another way to supplement food or feed is taken when free radicals are removed by adsorption from the organism. It uses the microporous structure of certain minerals. For example, zeolite crystals have tiny cavities and channels, which is due to their ability to absorb free radicals. In this case, other harmful substances are removed.
  • the mineral clinoptiolite is a component of the product Phytosorb, which is used as a toxin binder for pig and cattle feed.
  • the animal feed additive Lucoton-T uses the absorption capacity of chitin and melanin. For a high absorption ability also phyllosilicates are known.
  • Phyllosilicates are salts of silicic acid in which the [SiO 4 ] tetrahedra are interlinked in one plane and thus form lattice lattices. Some of the silicon atoms are replaced by magnesium or aluminum. One differentiates one-, two-, three- and four-layered silicates.
  • the layered silicates include, for example, kaolonite, talc, clay or mica. Compositions comprising phyllosilicates and biomass terrestrial fungi, in particular the genus Ganoderma, have not been described.
  • 1.3- ⁇ -glucans stimulation of the non-specific defense system, increase in the production of immunoglobulin A (IgA)
  • IgA immunoglobulin A
  • the antioxidant effect of spirulina is important for use as a dietary supplement (JANA 2002).
  • JANA 2002 An indication that the antioxidant effect can be synergistically enhanced by combination with extracts of Ganoderma species is not found in the prior art.
  • Known is only the combination of hot water extracts of G. lucidum with hot-water extracts of spirulina (JP 2002 24 9437 A).
  • Inorganic UV filters are microfine particles of titanium dioxide or zinc oxide, which reflect the incident UV light. They cover the longer-wave UV-A as well as the shorter-wave UV-B range and are mainly used in sunscreens with a very high sun protection factor. Because these pigments are white, they can cause an undesirable whitening effect on the skin at higher concentrations. To prevent this, the pigment particles are reduced to about 200 nanometers - thus they are transparent.
  • UV light When exposed to ultraviolet (UV) light, photocatalytic substances such as titanium dioxide (TiO 2 ;) absorb UV radiation to a high degree. In the reaction of titanium dioxide with UV radiation, however, free radicals are formed in the presence of water and oxygen. This photocatalytic effect is used for various commercial applications such as water and air purification and gives surfaces self-cleaning, antimicrobial and anti-algae properties (DE199623055). In the photocatalytic reaction, very reactive free radicals are formed, which form a strong Chem. Res., Vol. 28, No. 12 (1995) 503 / D. Bruemann: Photocatalytic Detoxification of Polluted Waters, The Handbook of Environmental Chemistry, Springer Verlag 1999, Volume 2, Part L, 285 - 351.
  • the object of the present invention is to find formulations with improved properties compared to the prior art which stimulate glucose metabolism, protect cells from damage and / or slow cell aging, stabilize the natural intestinal flora and bind toxins and which can therefore be used as food or feed and / or as a food or feed supplement and / or as cosmetics and / or as medicaments for therapeutic and prophylactic applications in humans and / or animals.
  • cosmetics in particular, a possibility should be found of combining UV protection and protection against premature skin aging by the residual UV radiation.
  • a composition comprising biomass and / or extracts of terrestrial fungi on the one hand and at least a) a layered silicate or an iron-rich raw material or b) fungal biopolymer complexes obtained by alkaline digestion from the cell walls of aphyllophoral basidiomycetes or c) biomasses obtainable from microalgae on the other hand , provided.
  • Terrestrial mushrooms have valuable ingredients that are suitable
  • Examples of terrestrial mushrooms with as yet insufficiently used ingredients are Auricularia auricula-judae-Judasohr
  • Grifola frondosa Maitake Hericium erinaceus - Hedgehog Tortoise Lentinula edodes - Shii-take Pleurotus ostreatus - Oyster Pear Pleurotus eryngii - Herbal Oyster.
  • the biomasses or the extracts of terrestrial fungi can be used.
  • the biomass of the terrestrial fungi all ingredients are supplied in a physiologically balanced ratio to the recipient organism. It has been found that it is particularly advantageous if the biomass is present in the form of microparticles or nanoparticles with an average diameter of 10 nm-10 ⁇ m.
  • the conversion of the biomass into microparticles or nanoparticles can be carried out according to a method known from WO 2004/075907 A2.
  • biomass and / or extracts of terrestrial mushrooms are mixed to form biomasses of marine organisms, obtainable from microalgae
  • the organism is supplemented, in addition to the ingredients of the selected terrestrial fungi, with proteins, special fatty acids, in particular cell protective omega-3 and omega-6 fatty acids, Minerals and vitamins as well as secondary ingredients supplied in a physiologically engineered ratio.
  • proteins special fatty acids, in particular cell protective omega-3 and omega-6 fatty acids, Minerals and vitamins as well as secondary ingredients supplied in a physiologically engineered ratio.
  • Both phyllosilicates as fungale Biopolymerkomplexe, biomass from marine organisms as well as commercial mineral mixtures have insufficient radical scavenging properties.
  • the preparation of the invention may contain biomass and / or extracts of terrestrial fungi and phyllosilicates, fungal biopolymer complexes and / or biomasses of microalgae and mineral mixtures as two, three or four combinations, and allows good protection against free radicals. In the combinations practically all mixing ratios are possible. This allows various applications that can not be achieved with the individual components.
  • the combination according to the invention provides animal and human with high-quality nutrients, ferments, active substances, growth and protective substances in a balanced ratio to fiber and thus strengthens the organism.
  • the glucans contained in the combination stimulate the immune system.
  • the dietary and probiotic properties of the combination stabilize the natural intestinal flora.
  • the composition can be chosen so that a fight against bacterial and viral pathogens by the known contained in G. pfeifferi antimicrobial agents is possible. Free radical damage, responsible for many aging processes of cells and organs, is diminished.
  • 1 part of the ethanolic extract of a Ganoderma species is used to 99 parts of the food.
  • special effects in terms of the supply of minerals and trace substances can be achieved.
  • the combination according to the invention with extracts of terrestrial fungi gives a preparation in which, with regard to the antioxidative properties, an immediate and a depot effect can be detected experimentally.
  • Inventive combinations of Ganoderma extracts with zeolites and other ion exchangers cause a synergistic antioxidant effect.
  • the microporous structure of the zeolites by their ability to absorb free radicals, promotes anti-oxidant activity. Incorporated substances such as selenium are reinforced in their effect. If the addition according to the invention of alcoholic extracts from a Ganoderma species of a combination of zeolites with important minerals and trace elements is added, both the radical scavenging activity can be improved and the assurance of a balanced balance of macro- and microelements in the organism can be achieved.
  • pfeifferi wherein the ethanol content can vary within the limits of 10 vol.% To 96 vol.%. It is also possible to use mixtures of alcohols and ethyl acetate as the extraction agent. It is also possible to use extracts obtained by treating a Ganoderma species with supercritical CO 2 .
  • extracts of 5 different Ganoderma species and / or extracts of other terrestrial fungi are mixed.
  • lipophilic extracts in particular the alcoholic extract of G. pfeifferi, contain active substances which stimulate the metabolism of healthy cells and reduce cell aging.
  • the aqueous extract of G. lucidum contains agents which activate phagocytosis by macrophages and stimulate the immune response.
  • the glucan content of the aqueous extract, which stimulates the immune system is lower in G.pfeifferi than in G. lucidum.
  • the combination of the ethanolic extract of G. pfeifferi and the aqueous extract of G. lucidum produces the desired effect.
  • 2 5 has a relatively low UV adsorption, by additions of biomasses of Ganoderma pfeifferi in the amount of 1 to 10% over the entire wavelength range of 250 to 400 nm comparable to the commercial preparations based on titanium dioxide, z. T. even has superior UV absorption (or reflection). It has proven to be particularly favorable when the biomass from Ganoderma species in the form of micro- and nanoparticles with
  • From the group of phyllosilicates can be used for the UV protection in a particularly advantageous manner kaolins, clays, bentonites and other clay mineral-containing rocks.
  • Non-clay mineral, but Fe-rich raw materials are used.
  • Gaps associated with individual clay mineral groups, such as in kaolins e.g. in the range 280 nm, can be safely avoided by the inventive combination with biomasses from Ganoderma species.
  • a clay particle-biomass particle contact network is essential.
  • the formation of this network can be influenced, for example, by: pH change
  • the process for the preparation of the preparations may be carried out in such a way that the clays dried and / or ground and / or separated and / or otherwise surface-treated, then with a water / oil emulsion, in which either the
  • Ratio 1: 10 to 1: 1 are mixed, and both fruiting body and the mycelium can be used for biomass and extract extraction.
  • Example 6 Examination of the influence of the locus flora on the mouse ear by preparations according to the invention according to Examples 1, 5 and 6
  • Example 8 Influence of grain size in phyllosilicates without permanent charge
  • the release was determined by means of paddle apparatus.
  • the particle dispersion to be tested is placed directly in the round-bottomed vessel and samples are taken at certain times.
  • the particles contained in the measuring solution are filtered off and discarded. You will be deprived of the further release process. To compensate, the sample volume is not replaced, so it can be assumed that the output ratio of particles to dispersion medium remains constant during the course of the experiment.
  • the filtrate is analyzed by UV spectrometry for its active ingredient content.
  • Membrane filters made of polyamide from Schleicher & Schuell (Dassel, Germany) with a pore size of 0.2 ⁇ m were used for the filtration.
  • adherent human cells HaCaT, DKFZ
  • adherent human cells HaCaT, DKFZ
  • the medium was replaced by 2 ml PBS and the Cells irradiated with a dose of 20mJ / cm 2 UV light (medical UVB, broadband, maximum 311nm).
  • the preparation was carried out under standard conditions as shown in the scheme ( Figure 5 extraction scheme for the production of mushroom extracts).
  • Ganoderma extracts can significantly reduce UV-induced damage to the keratinocytes.
  • FIG. 6 LDH release from HaCaT cells of Ganoderma extracts after irradiation with medical UVB
  • Confluent FL cells were treated with mitomycin C to inhibit growth (described in detail in Example 15).
  • the cell cultures were fed from the 20th to the 30th h with a commercial medium (Dulbecco's Minimal Essential Medium with 10% calf serum), to which was added 0.025 mg of an ethanolic extract of G. pfeifferi per ml culture medium.
  • G. lucidum (not shown) has an opposite effect.
  • the cells die here already after 96h.
  • Example 15 Detection of the stimulating effect by the preparations according to the invention on stationary cell cultures by determining the protein content of the cells
  • Confluent FL cells are treated with mitomycin C to inhibit growth as follows: pour medium from tissue culture flask, wash cell lawn 2 times 5 times with PBS, 5 ml commercial medium (DulbeccoMEM + antibiotics + 10% fetal calf serum + 1% L glutamine) Add 50 ⁇ l mitomycin C (50 ⁇ g) to the cells, incubate for 2h at 37 ° C and 5% CÜ 2 in the incubator, drain the mixture and wash 3 times with PBS. The cells were then trypsinized, in a cell density of 2x10 5 / ml medium in
  • the protein determination was carried out with Roti-Nanoquant and a B SA standard as lo calibration solution.
  • the protein concentration was determined on the basis of the quotients from the B SA standard curve.
  • the G. pfeifferi hot extract used caused a very marked stimulation of the cell metabolism.
  • the protein content of the cell increased by 20%.
  • 25 ⁇ g / ml have practically no influence, only doubling the concentration to 50 ⁇ g / ml causes an increase in protein production by 5%. In the examination of the finished preparation even a lower protein content was observed.
  • FIG. 9 Protein content ( ⁇ g / ml) of cells after cultivation with and without G. pfeifferi in comparison with G. lucidum and ginseng
  • HaCaT, DKFZ adherent human cells
  • the medium was replaced by 2 ml PBS and the cells irradiated with a dose of 20mJ / cm 2 UV light (medical UVB, broadband, maximum 311nm).
  • PBS extracts of Ganoderma species were dissolved at a concentration of 10 ⁇ g / ml (Extr. 1) or 0.01 ⁇ g / ml (Extr. 2).
  • the PBS and the extract dissolved therein was removed and replaced with RPMI medium with 0.01% BSA (bovine serum albumin).
  • BSA bovine serum albumin
  • FIG. 10 LDH release from HaCaT cells after irradiation with medical UVB (broadband)
  • the extracts used were able to reduce the damage caused to the cells by UV radiation (FIG. 9).
  • the Ganoderma-Extr. 1 (A) gives up to a 5-fold protective effect.
  • the less concentrated Ganoderma 5 Extr. 2 ( ⁇ ) an approximately 1.5 fold protective effect for the cells could be detected.
  • Titanium dioxide which is very commonly used in sunscreens, enhanced by the photocatalytic effect, the damaging effect of UV rays. Titanium dioxide was used in direct comparison as a suspension of titanium dioxide nanoparticles in PBS with a dose of 3 ⁇ g / cm 2 . (Fig. 10)
  • Example 18 Detection of the protective effect of the preparations according to the invention on skin cells in comparison to TiO 2
  • the experimental setup was modified in that in the beam path either only a quartz plate (control) or a quartz plate with a corresponding preparation of the invention (clay mineral plus biomass, see Example.10 production) or 20 TiO 2 (in the form a nanoparticle suspension).
  • the quartz plate alone does not absorb any UV light in this area.
  • the PBS was removed and replaced with RPMI medium containing 8% FCS.
  • the morphological development of the cells and the release of the cytoplasmic enzyme lactate dehydrogenase were followed.
  • FIG. 11 LDH release from HaCaT cells after irradiation with medical UVB (broadband), preparation from G. pfeifferi and Frieders clay in comparison to TiO 2
  • Lactate release is increased under the influence of TiO 2 over the control
  • FIG. 7 LDH release from HaCaT cells after irradiation with medical UVB (broadband). Preparation of G.pfeiferi and bentonite Garfield ⁇ 6.3 ⁇ m)
  • TiO 2 has an unfavorable effect on skin cells even in this experimental setup in which the photocatalytic effect is not detected.
  • Lactate release as a measure of UV-induced damage under the influence of the preparation according to the invention (Example 1 in Fig. 11: Preparation obtainable from G.pfeifferi and Friedlander Ton;
  • the UV transmission was measured with the UV photometer Analytik Jena AG SPECORD 50.
  • Example 20 Investigation of the compositions according to the invention based on biomass of microalgae of the genus Spirulina enriched with selenium in biologically bound form (selenium spirulina) with and without digestion by heat and pressure
  • the determination of the antioxidative properties was carried out by thin-layer chromatography (SIEVERS A et al., (2002) Simple thin-layer chromatography test for antioxidative compounds using DPPH assay, CBS Camag bibliography Service 88: 14-15) and photometrically (MOLYNEUX P (2004).
  • Selenium spirulana with a selenium content of 7.5 mg selenium / 100 g has no detectable radical scavenging properties with these test systems.
  • the thin-layer chromatographic detection is very sensitive. Concentrations> 0.005% of an antioxidant substance are detectable. By adding 1% G. lucidum extract, an immediately detectable radical scavenging activity of 94% is achieved. An addition of 10% causes a radical scavenging activity of 95.1%.
  • Microalgae of the genus Spirulina enriched with zinc in biologically bound form (zinc spirulina)
  • Zinc spirulina has a radical scavenging capacity of 20.3%.
  • 1% ethanolic extracts of G. lucidum or G. pfeifferi immediately detectable radical scavenging activities of 74, 1, by adding 10% of the extracts 92.3% are achieved.
  • Biomass obtainable by cultivating microalgae of the genus Chlamydomonas, has a radical trapping property of 14.8%. By adding 1 30% ethanolic extract of G. lucidum or G. pfeifferi, immediately detectable radical scavenging activities of 77, 3 are achieved by adding 10% of the extracts> 90%.
  • Example 20 Biomass, obtainable by culturing microalgae of the genus Porphyridium, have only a low radical scavenging property of 4%. By adding 1% ethanolic extracts of G. lucidum or G. pfeifferi, immediately detectable radical scavenging activities of 40, 7 are achieved by adding 10% of 94%.
  • the complex was recovered by alkaline digestion from the cell wall according to Example 29.
  • the preparation has the following advantageous application properties: Bioadsorption of heavy metals and toxins, but no adsorption more vital
  • Macro- and microelements strengthening the immune system by stimulating the nonspecific defense system and stimulating the anti-inflammatory cytokines
  • the complex has no doping with Ganoderma extract on the detectable with the methodology of Example 20 radical scavenging properties (radical scavenging activity 5.3%). This disadvantage is remedied by adding 1% or 10% of an ethanolic extract from G. lucidum. The radical scavenging activity thereby increases to 50 or 93%.
  • Example 25 Fungal biopolymer complex obtainable from oyster mushroom substrates (Pleurotus ostreatus)
  • the biopolymer complex was recovered by alkaline digestion from the cell walls in analogy to Example 29.
  • the investigation of antioxidative properties was carried out according to the method of Example 20.
  • the biopolymer complex has a radical scavenging capacity of 5.7%.
  • 1% ethanolic extract of G. lucidum By adding 1% ethanolic extract of G. lucidum, immediately detectable radical scavenging activities of 42.2 are achieved, with the addition of 10% of the extracts 89.6%.
  • a radical scavenging activity is advantageous, which is achieved by addition of 1 to 10% of an ethanolic extract of G. lucidum (Table 6).
  • Example 27 Improvement of radical scavenging properties of fish feed
  • % of a Ganoderma extract from 91.4 to 96.4 and 98.9%, respectively.
  • 50g dried and ground oyster mushroom (Pilzhof Krummensee) are in a three-necked flask with stirrer, thermometer and reflux condenser with 500 ml dest. Added water and heated to 60 ° C with stirring. Then add 3.5 g of sodium hydroxide (Merck, for analysis) in 50 ml of dist. Dissolved water and heated the mixture for three hours to 90 to
  • Ganoderma pfeifferi fruiting bodies were obtained by wild collection in Schlosspark Ludwigsburg in the years 1997-2005. In addition, cultured fruiting bodies, which were obtained by the company GAMU GmbH, Krefeld in April 2004, were used. 2 g of dried mushrooms (Ganoderma pfeifferi) are ground and treated at room temperature with 20 ml of ethanol (Applichem, denatured, for analysis). After 24 hours of cold extraction at room temperature, the fungi are squeezed through a filter and the extract is filtered through a round filter. This gives 12 ml of yellow-colored extract.
  • the pulverized fruit body was mixed in a paper tube with ethanol 80% (v / v) and then extracted by the Soxhlet method for about 24 h.
  • the weight was 12.5 g for both Ganoderma lucidum and G. pfeifferi.
  • the recovered liquid extracts were concentrated and freeze-dried.
  • Extraction was carried out using extractor ASE 200 (Dionex, Sunnyvale, CA, USA). In each case, 2 g of powdered mushroom fruit bodies with 15 g of sea sand were weighed into a paper tube. Extraction was carried out at a pressure of 2000 psi (pounds per square inch) with a service life (residence time of the solvent in the extraction cell at each extraction step) of 2 minutes. The extractant used was EtOH 80% (v / v) at an extraction temperature of 60 ° C.

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Abstract

L'invention concerne des compositions à base de biomasses et/ou d'extraits d'au moins un champignon terrestre, de préférence du type de l'espèce ganoderme d'une part et d'au moins un phyllosilicate, d'une matière première riche en fer, de biomasses, obtenues à partir de microalgues, de complexes biopolymères fongiques ou de mélanges de minéraux, d'autre part. Ces compostions s'utilisent entre autres comme agents protecteurs contre les U.V., à base de substances naturelles, préviennent le vieillissement cellulaire et possèdent un effet anti-vieillissement. On utilise de préférence des biomasses sous forme de micro particules et de nanoparticules. D'autres applications de ces compositions concernent les nutriments complémentaires, des agents phytotoniques, la protection contre les radicaux libres ou des médicaments.
EP06792472A 2005-06-30 2006-06-30 Preparations a base de substances naturelles Withdrawn EP1901757A2 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE102005031363A DE102005031363A1 (de) 2005-06-30 2005-06-30 Mittel mit Anti-Aging-Effekt
DE102005031364A DE102005031364A1 (de) 2005-06-30 2005-06-30 Nahrungsergänzungsmittel mit verbesserten antioxidativen Eigenschaften
DE102005031362A DE102005031362A1 (de) 2005-06-30 2005-06-30 Präparate auf der Basis von Naturstoffen
PCT/EP2006/063773 WO2007003613A2 (fr) 2005-06-30 2006-06-30 Preparations a base de substances naturelles

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EP1901757A2 true EP1901757A2 (fr) 2008-03-26

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DE102014200922A1 (de) * 2014-01-20 2015-07-23 Fim Biotech Gmbh Verwendung von Tonmineral als Futterzusatzstoff und/oder als Ergänzungsfutter für aquatische Organismen
CN113208987A (zh) * 2020-09-07 2021-08-06 北京工商大学 抗衰修复的大米灵芝菌复合发酵物及其制备方法和应用

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ATE243042T1 (de) * 1999-03-09 2003-07-15 Ganomycin Ges Fuer Biomedizini Biologisch aktive verbindungen aus ganoderma pfeifferi dms 13239
EP1603409A2 (fr) * 2003-02-27 2005-12-14 Ernst-Moritz-Arndt-Universität Greifswald Compositions salutaires pour la sante et composees de champignons contenant des lipides et de thiocyanates

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