EP1986509A2 - Arabinogalactan-protein mit der eigenschaft, fette zu absorbieren, und verfahren zur gewinnung dieses arabinogalactan-proteins - Google Patents

Arabinogalactan-protein mit der eigenschaft, fette zu absorbieren, und verfahren zur gewinnung dieses arabinogalactan-proteins

Info

Publication number
EP1986509A2
EP1986509A2 EP06849571A EP06849571A EP1986509A2 EP 1986509 A2 EP1986509 A2 EP 1986509A2 EP 06849571 A EP06849571 A EP 06849571A EP 06849571 A EP06849571 A EP 06849571A EP 1986509 A2 EP1986509 A2 EP 1986509A2
Authority
EP
European Patent Office
Prior art keywords
arabinogalactan protein
protein
water
agp
cactus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP06849571A
Other languages
English (en)
French (fr)
Inventor
Mohamed Elaghdef Malainine
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universite Cadi Ayyad
Original Assignee
Universite Cadi Ayyad
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universite Cadi Ayyad filed Critical Universite Cadi Ayyad
Publication of EP1986509A2 publication Critical patent/EP1986509A2/de
Withdrawn legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/33—Cactaceae (Cactus family), e.g. pricklypear or Cereus
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/20—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents
    • A23L29/206—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of vegetable origin
    • A23L29/25—Exudates, e.g. gum arabic, gum acacia, gum karaya or tragacanth
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
    • A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/06—Antihyperlipidemics

Definitions

  • AGP Arabinogalactan (s) protein (s)
  • AG Arabinogalactan (s)
  • the present invention is related to methods of weight control and cholesterol reduction without the need for restriction of dietary energy intake.
  • the former even with added fiber, has a limited capacity for fixation under physiological conditions.
  • the change in pH in the intestines there is a phenomenon of lipid re-release, which frees them from chitosan fixation. The result is that the lipids can be assimilated by the body.
  • the nopal powder As for the nopal powder, it is a heterogeneous material, of natural origin, therefore of variable composition over time, but having proved a capacity of attachment of fat equivalent or even superior to that of chitosan.
  • the problem is to find an active ingredient that is:
  • AGPs are very common in the plant world. This combination of polysaccharides and proteins seems to have important physiological functions for plants.
  • the saccharine part (AG) is also of great commercial importance, hence a scientific interest marked by the number of publications and patents that concern them.
  • the structure of AGs has been extensively studied. See, for example, E.G., Timell, Adv. Carbohydrate Chem., Vol. 20, pp. 409-483 (1965).
  • Arabinogalactans are polysaccharides whose backbones consist of beta (1,3) -galactan chains, densely branched by side chains consisting of arabinose, galactose and often other minor residues.
  • AGs are gum arabic, exuding Acacia Senegal.
  • a more recent source of relatively pure GAs is the extract of Larex Occidentalis wood.
  • These AGs are used in the food and cosmetics industry as a texturizing agent, gelling agent and as an emulsifier facilitating the water - oil mixture.
  • the said AGP may be in any galenical or commercial form, solid or dissolved in water.
  • the invention aims to propose a method based on a product, and a process for obtaining such a product, having the property of fixing fats. It aims to provide a product that can be safely ingested (that is to say not harmful) and has the property of fixing fats in vivo to remove them from digestion.
  • the invention aims at first, a method for absorbing, fixing and then eliminating dietary fat ingested before assimilation by the body using an AGP extracted from the cactus mucilage.
  • AGP extracted from cacti mucilage behaves similarly, given the structure of the polysaccharide that constitutes it.
  • the AGP extracted from the cactus, object of this invention can be in any galenic or commercial form, solid or dissolved in water. It can be packaged alone, in the form of tablets, capsules, soft or rigid capsules or just powder to dissolve in a drink (water or other soda, fruit juice, etc.) or to sprinkle on a food. It is then preferably ingested during a meal.
  • the invention is therefore extended to any preparation, containing AGP extracted from cactus mucilage, having the property of fixing ingested dietary fats, so as to minimize their assimilation. It is also the mode of extraction of this AGP from the mucilage of a cactus genus platyopuntia, by a process to preserve the association polysaccharide-protein association responsible for the activity observed.
  • the maceration must take place quickly enough, and at low temperature.
  • the maceration is immediately followed by a solid liquid separation.
  • the separation is advantageously carried out by centrifugation and filtration.
  • the resulting AGP solution is then purified.
  • This purification is advantageously obtained according to the invention, by subjecting it to dialysis against distilled water, to eliminate salts, and molecules of small molecular weight.
  • the purified AGP solution is then concentrated.
  • the concentration is advantageously obtained according to the invention, in an enclosure under partial vacuum and at low temperature.
  • the concentrated solution of AGP is then dried.
  • the elimination of water can be done in two advantageous ways according to the invention. Either by lyophilization or by atomization.
  • the AGP produced according to the invention is compared with commercial products having the property of fixing fats.
  • test tubes each containing 7.5 ml of 0.1N HCl and 6 g (mass M) of sunflower oil are prepared.
  • 100 mg (mass M1) of the test preparation (Neopuntia ® type cactus powder, Absorbitol ® type chitosan, commercial Larex UF ® type ag or Acacia gum type AGP or prepared according to the invention are placed in each tube. ).
  • the solutions obtained are mixed vigorously, shaking the tube by hand for 30s, and placed in an oven at 37 ° C for an incubation of 2 hours.
  • 0.5 ml of a pH 7.3 buffer solution are then added to each of the tubes.
  • the tubes are returned to the oven at 37 ° C. for 3 hours. They are then centrifuged for 5 minutes at 2000 rpm.
  • the supernatant fat is then removed and weighed (mass M2), and the ratio of the mass of fatty substance fixed by the preparation (M-M2) to the preparation mass used (Ml), called the attachment ratio, is calculated.
  • the extraction method disclosed by this invention aims to prepare a high molecular weight arabinogalactan protein without causing breakdown of the bonds between the polysaccharide moiety and the protein moiety.
  • the resulting brilliant juice is then dialyzed against purified water, on 10,000 Dalton cutoff membrane, and then concentrated in the falling film evaporator ADF for 90 minutes. 1 liter of concentrate is taken to make it freeze-dried. The remainder is passed on the atomization tower working at a hot air temperature of 190 ° C. In both cases (lyophilization or atomization), the AGP powder obtained is light yellow in color.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Engineering & Computer Science (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Nutrition Science (AREA)
  • Mycology (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Medical Informatics (AREA)
  • Dispersion Chemistry (AREA)
  • Epidemiology (AREA)
  • Microbiology (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Biotechnology (AREA)
  • Botany (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Obesity (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Hematology (AREA)
  • Diabetes (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
EP06849571A 2005-12-15 2006-12-13 Arabinogalactan-protein mit der eigenschaft, fette zu absorbieren, und verfahren zur gewinnung dieses arabinogalactan-proteins Withdrawn EP1986509A2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
MA28668A MA28714B1 (fr) 2005-12-15 2005-12-15 Une arabinogalactane proteine ayant la propriete d'absorber les graisses et le procede d'obtention de cette arabinogalactane proteine
PCT/MA2006/000005 WO2007100236A2 (fr) 2005-12-15 2006-12-13 Une arabinogalactane proteine ayant la propriete d’absorber les graisses et le procede d’obtention de cette arabinogalactane proteine

Publications (1)

Publication Number Publication Date
EP1986509A2 true EP1986509A2 (de) 2008-11-05

Family

ID=38459451

Family Applications (1)

Application Number Title Priority Date Filing Date
EP06849571A Withdrawn EP1986509A2 (de) 2005-12-15 2006-12-13 Arabinogalactan-protein mit der eigenschaft, fette zu absorbieren, und verfahren zur gewinnung dieses arabinogalactan-proteins

Country Status (6)

Country Link
US (1) US20090253621A1 (de)
EP (1) EP1986509A2 (de)
JP (1) JP2009519327A (de)
CA (1) CA2632713A1 (de)
MA (1) MA28714B1 (de)
WO (1) WO2007100236A2 (de)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1051560C (zh) * 1990-03-07 2000-04-19 普利瓦药物、化学、食品、化妆品工业公司 抗菌素与络合金属盐的螯合物的制备方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4223023A (en) * 1978-10-12 1980-09-16 Ivan Furda Nonabsorbable lipid binder
FR2823423B1 (fr) * 2001-04-11 2004-08-27 Bio Serae Laboratoires Preparation a base de cactaceae ayant la propriete de fixer les graisses, et procede d'obtention d'une telle preparation
CA2519969C (en) * 2003-04-07 2013-03-19 Phillips Hydrocolloids Research Limited Modified gum arabic
EP1612225A4 (de) * 2003-04-09 2008-01-02 Phillips Hydrocolloids Res Ltd Modifiziertes akaziengummi und verwendung davon

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO2007100236A2 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1051560C (zh) * 1990-03-07 2000-04-19 普利瓦药物、化学、食品、化妆品工业公司 抗菌素与络合金属盐的螯合物的制备方法

Also Published As

Publication number Publication date
MA28714B1 (fr) 2007-07-02
JP2009519327A (ja) 2009-05-14
WO2007100236A3 (fr) 2007-12-21
US20090253621A1 (en) 2009-10-08
WO2007100236A2 (fr) 2007-09-07
CA2632713A1 (fr) 2007-09-07

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