EP1987148A4 - Dedifferenzierung von astrozyten zu neuralen stammzellen unter verwendung von bmi-1 - Google Patents

Dedifferenzierung von astrozyten zu neuralen stammzellen unter verwendung von bmi-1

Info

Publication number
EP1987148A4
EP1987148A4 EP06732855A EP06732855A EP1987148A4 EP 1987148 A4 EP1987148 A4 EP 1987148A4 EP 06732855 A EP06732855 A EP 06732855A EP 06732855 A EP06732855 A EP 06732855A EP 1987148 A4 EP1987148 A4 EP 1987148A4
Authority
EP
European Patent Office
Prior art keywords
astrocytes
bmi
differentiation
stem cell
neural stem
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP06732855A
Other languages
English (en)
French (fr)
Other versions
EP1987148A1 (de
Inventor
Seungkwon You
Jai Hee Moon
Byung Sun Yoon
Seung Jun Yoo
Ki Dong Kim
Isaac Maeng
Gyuman Park
Eun Kyung Jun
Sung Sik Kwak
Bona Kim
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
IMGEN CO Ltd
Original Assignee
IMGEN CO Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by IMGEN CO Ltd filed Critical IMGEN CO Ltd
Publication of EP1987148A1 publication Critical patent/EP1987148A1/de
Publication of EP1987148A4 publication Critical patent/EP1987148A4/de
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/82—Translation products from oncogenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0623—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
    • C12N2501/392—Sexual steroids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/08—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from cells of the nervous system

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Oncology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP06732855A 2006-02-27 2006-04-12 Dedifferenzierung von astrozyten zu neuralen stammzellen unter verwendung von bmi-1 Withdrawn EP1987148A4 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20060019018 2006-02-27
PCT/KR2006/001350 WO2007097494A1 (en) 2006-02-27 2006-04-12 De-differentiation of astrocytes into neural stem cell using bmi-1

Publications (2)

Publication Number Publication Date
EP1987148A1 EP1987148A1 (de) 2008-11-05
EP1987148A4 true EP1987148A4 (de) 2009-08-05

Family

ID=38437522

Family Applications (1)

Application Number Title Priority Date Filing Date
EP06732855A Withdrawn EP1987148A4 (de) 2006-02-27 2006-04-12 Dedifferenzierung von astrozyten zu neuralen stammzellen unter verwendung von bmi-1

Country Status (5)

Country Link
EP (1) EP1987148A4 (de)
JP (1) JP2009528050A (de)
KR (1) KR100844971B1 (de)
CN (1) CN101389761A (de)
WO (1) WO2007097494A1 (de)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8129187B2 (en) 2005-12-13 2012-03-06 Kyoto University Somatic cell reprogramming by retroviral vectors encoding Oct3/4. Klf4, c-Myc and Sox2
US8278104B2 (en) 2005-12-13 2012-10-02 Kyoto University Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2
US20090227032A1 (en) 2005-12-13 2009-09-10 Kyoto University Nuclear reprogramming factor and induced pluripotent stem cells
EP2206724A1 (de) 2005-12-13 2010-07-14 Kyoto University Nukleärer Reprogramierungsfaktor
JP2008307007A (ja) 2007-06-15 2008-12-25 Bayer Schering Pharma Ag 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞
US9213999B2 (en) 2007-06-15 2015-12-15 Kyoto University Providing iPSCs to a customer
KR101532442B1 (ko) 2007-12-10 2015-06-29 고쿠리츠 다이가쿠 호진 교토 다이가쿠 효율적인 핵 초기화 방법
US9683232B2 (en) 2007-12-10 2017-06-20 Kyoto University Efficient method for nuclear reprogramming
EP2242838A1 (de) * 2007-12-17 2010-10-27 GliaMed, Inc. Stammzellen-ähnliche zellen und verfahren zur umprogrammierung adulter somatischer säugerzellen
JP5346925B2 (ja) 2008-05-02 2013-11-20 国立大学法人京都大学 核初期化方法
US20140038291A1 (en) 2009-10-31 2014-02-06 New World Laboratories Inc. Methods for reprogramming cells and uses thereof
US9453205B2 (en) 2009-10-31 2016-09-27 Genesis Technologies Limited Methods for reprogramming cells and uses thereof
LT2494039T (lt) 2009-10-31 2019-07-25 Genesis Technologies Limited Ląstelių perprogramavimo būdai ir jų panaudojimas
KR101158402B1 (ko) * 2009-12-30 2012-06-22 주식회사 일진케이제이 Ｂｍｉ１ 및 Ｏｃｔ４를 이용하여 체세포로부터 배아줄기세포 유사세포로의 역분화를 유도하는 조성물 및 이를 이용한 배아줄기세포 유사세포의 제조방법
JP2011135864A (ja) 2009-12-30 2011-07-14 Korea Univ Research & Business Foundation Ｏｃｔ４及びＢｍｉ１、またはその上位調節子を用いて体細胞から胚幹細胞類似細胞への逆分化を誘導する組成物及びこれを用いた胚幹細胞類似細胞の製造方法
CN102242146B (zh) * 2010-05-10 2015-11-25 高丽大学校产学协力团 组合物和用其产生诱导全能干细胞的方法
CN106381280B (zh) 2010-05-13 2020-08-04 加州大学董事会 用于诱导人类多能干细胞的方法和组合物
CN106754728B (zh) * 2016-12-30 2020-05-15 中国人民解放军总医院 后肾间充质细胞系及其制备方法与应用
CN109674809B (zh) * 2018-12-27 2022-08-09 吉林大学 一种包括miR-124-3P的组合物及其在诱导神经元形成的药物中的应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030022375A1 (en) * 2001-07-05 2003-01-30 Akira Itoh Vector for reversible gene integration

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19516776A1 (de) * 1995-05-10 1996-11-14 Boehringer Ingelheim Int Chromatin-Regulatorgene
US6897061B1 (en) * 2000-06-16 2005-05-24 Spinal Cord Society Transdifferentiation of glial cells
US6919204B2 (en) * 2000-09-29 2005-07-19 Sangamo Biosciences, Inc. Modulation of gene expression using localization domains
KR20010099203A (ko) * 2001-09-11 2001-11-09 이상훈 신경 줄기세포를 도파민성 신경세포로 분화시키는 방법

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030022375A1 (en) * 2001-07-05 2003-01-30 Akira Itoh Vector for reversible gene integration

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
ALKEMA M J ET AL: "CHARACTERIZATION AND CHROMOSOMAL LOCALIZATION OF THE HUMAN PROTO-ONCOGENE BMI-1", HUMAN MOLECULAR GENETICS, OXFORD UNIVERSITY PRESS, SURREY, vol. 2, no. 10, 1 October 1993 (1993-10-01), pages 1597 - 1603, XP000676533, ISSN: 0964-6906 *
JOANNIDES ALEXIS ET AL: "Efficient generation of neural precursors from adult human skin: astrocytes promote neurogenesis from skin-derived stem cells.", LANCET 2004 JUL 10-16, vol. 364, no. 9429, 10 July 2004 (2004-07-10), pages 172 - 178, XP002533122, ISSN: 1474-547X *
MOLOFSKY A V ET AL: "Bmi-1 dependence distinguishes neural stem cell self-renewal from progenitor proliferation", NATURE, NATURE PUBLISHING GROUP, LONDON, UK, vol. 425, no. 6961, 30 October 2003 (2003-10-30), pages 962 - 967, XP002324138, ISSN: 0028-0836 *
MOON J H ET AL: "Induction of neural stem cell-like cells (NSCLCs) from mouse astrocytes by Bmi1", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US, vol. 371, no. 2, 27 June 2008 (2008-06-27), pages 267 - 272, XP022659023, ISSN: 0006-291X, [retrieved on 20080424] *
PARK IN-KYUNG ET AL: "Bmi1, stem cells, and senescence regulation.", THE JOURNAL OF CLINICAL INVESTIGATION JAN 2004, vol. 113, no. 2, January 2004 (2004-01-01), pages 175 - 179, XP002533123, ISSN: 0021-9738 *
See also references of WO2007097494A1 *
ZENCAK DUSAN ET AL: "Bmi1 loss produces an increase in astroglial cells and a decrease in neural stem cell population and proliferation.", THE JOURNAL OF NEUROSCIENCE : THE OFFICIAL JOURNAL OF THE SOCIETY FOR NEUROSCIENCE 15 JUN 2005, vol. 25, no. 24, 15 June 2005 (2005-06-15), pages 5774 - 5783, XP002533121, ISSN: 1529-2401 *

Also Published As

Publication number Publication date
KR20070089089A (ko) 2007-08-30
JP2009528050A (ja) 2009-08-06
CN101389761A (zh) 2009-03-18
WO2007097494A1 (en) 2007-08-30
EP1987148A1 (de) 2008-11-05
KR100844971B1 (ko) 2008-07-09

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