EP2041165A2 - Neue anwendungen für staphylococcus-aureus-sbi-protein - Google Patents
Neue anwendungen für staphylococcus-aureus-sbi-proteinInfo
- Publication number
- EP2041165A2 EP2041165A2 EP07733038A EP07733038A EP2041165A2 EP 2041165 A2 EP2041165 A2 EP 2041165A2 EP 07733038 A EP07733038 A EP 07733038A EP 07733038 A EP07733038 A EP 07733038A EP 2041165 A2 EP2041165 A2 EP 2041165A2
- Authority
- EP
- European Patent Office
- Prior art keywords
- sbi
- complement
- binding
- protein
- binding protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/472—Complement proteins, e.g. anaphylatoxin, C3a, C5a
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/1703—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- A61K38/1709—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/305—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F)
- C07K14/31—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F) from Staphylococcus (G)
Definitions
- the system may contain "responder" cells capable of responding to one or more products of complement activation, such as anaphylatoxins (C3a and C5a) or opsonised targets (which may or may not be cells) carrying opsonins such as C3b.
- responder cells are typically cells of the immune system and include basophils, neutrophils, mast cells, and macrophages .
- the invention also provides use of a complement-binding protein comprising a Sbi-III domain capable of binding to C3 protein and/or a Sbi-IV domain capable of binding to C3 protein in the preparation of a medicament for the treatment of an inflammatory condition.
- the complement-binding protein may comprise a Sbi- III-IV polypeptide.
- Inhibition of B cell proliferation and/or antibody production may be useful in any inflammatory condition in which antibody production contributes to the pathogenesis or symptoms experienced. These include the inflammatory conditions set out above .
- either the C3 protein or fragment or the complement-binding protein is preferably immobilised on a solid phase, and contacted with a sample containing the other protein.
- the candidate compound under test may be introduced before, concurrently with, or after the sample containing the other protein component.
- the invention further provides methods for detection or isolation of C3 and fragments thereof, e.g. in or from biological samples.
- a method of detecting C3 or a fragment thereof in a sample, or isolating C3 or a fragment thereof from a sample comprising contacting said sample with a complement-binding protein comprising a Sbi-III domain capable of binding to said C3 or fragment thereof and/or a Sbi-IV domain capable of binding to said C3 or fragment thereof.
- the method will typically comprise a step of removing any bound impurities or contaminants, e.g. by suitable washing.
- the invention further provides a nucleic acid encoding a complement-binding protein comprising a Sbi-III domain capable of binding C3 protein, and/or a Sbi-IV domain capable of binding C3 protein, in the absence of a functional Sbi-I domain and/or a functional Sbi-II domain.
- the invention further provides" a complement-binding protein comprising a Sbi-III domain capable of binding to C3 protein and/or a Sbi-IV domain capable of binding to C3 protein, or a nucleic acid encoding the same, or a vector or host cell containing such a nucleic acid, for use in a method of medical treatment.
- the complement-binding protein may comprise a Sbi-III domain in the absence of Sbi-IV, or may comprise a Sbi-IV domain in the absence of Sbi-III, or may comprise a Sbi-III domain and a Sbi-IV domain.
- it may comprise a Sbi-III-IV polypeptide.
- FIG. 2a Experimental scattering curve of Sbi-E with error bars (1) and the scattering from the ab in ⁇ tio model (2) .
- the plot displays the logarithm of the scattering intensity as a function of momentum transfer s.
- the distance distribution function of Sbi-E calculated from the experimental data by the program GNOM is presented in the inset figure.
- 2b A typical ab initio bead model of Sbi-E determined by DAMMIN 14 .
- the 4 major globular domains are depicted with Roman numerals.
- a homology model of Sbi domains I and II (shown in surface and ribbon representations) is superimposed onto the ab initio SAXS structure.
- Figure 5a Sensorgrams showing the relative binding between sensorchip imised Sbi-III-IV (-1200 RU) and complement C3 derivatives: native 03, C3b, iC3b, C3c, C3dg, methylamine-reacted C3 (03(NHCH 3 )), iC3 (NHCH 3 ) and trypsinised 03 (NHCH 3 ) (denoted
- Either type of complement-binding protein may possess further Sbi sequences derived from upstream (N-terminal) of the Sbi-III domain, including a Sbi-II domain, and/or a Sbi-I domain. Additionally or alternatively, they may possess further Sbi sequence derived from downstream (C-terminal) of the Sbi-IV domain, such as the cell wall-spanning sequence and/or Y region. However it is generally preferred that they do not possess Sbi-I or Sbi-II domains, or the cell wall-spanning or Y domains.
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Immunology (AREA)
- Marine Sciences & Fisheries (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Toxicology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB0610776A GB0610776D0 (en) | 2006-05-31 | 2006-05-31 | Novel applications for staphylococcus aureus Sbi protein |
| PCT/GB2007/002022 WO2007138328A2 (en) | 2006-05-31 | 2007-05-31 | Novel applications for staphylococcus aureus sbi protein |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| EP2041165A2 true EP2041165A2 (de) | 2009-04-01 |
Family
ID=36694713
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP07733038A Withdrawn EP2041165A2 (de) | 2006-05-31 | 2007-05-31 | Neue anwendungen für staphylococcus-aureus-sbi-protein |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP2041165A2 (de) |
| AU (1) | AU2007266813A1 (de) |
| CA (1) | CA2653668A1 (de) |
| GB (1) | GB0610776D0 (de) |
| WO (1) | WO2007138328A2 (de) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| HUE026001T2 (en) * | 2007-02-05 | 2016-04-28 | Apellis Pharmaceuticals Inc | Compstatin analogues for use in the treatment of the inflammatory condition of the respiratory system |
| US8592555B2 (en) | 2008-08-11 | 2013-11-26 | Emd Millipore Corporation | Immunoglobulin-binding proteins with improved specificity |
| SG195555A1 (en) | 2008-12-24 | 2013-12-30 | Emd Millipore Corp | Caustic stable chromatography ligands |
| GB201016403D0 (en) * | 2010-09-29 | 2010-11-10 | Bath Ventures | Novel interaction between staphylococcus aureus Sbi and C3d protiens |
| WO2012095519A1 (en) * | 2011-01-13 | 2012-07-19 | Leibniz-Institut Für Naturstoff-Forschung Und Infektionsbiologie | Potent inhibitors of complement activation |
| SG186552A1 (en) | 2011-06-08 | 2013-01-30 | Emd Millipore Corp | Chromatography matrices including novel staphylococcus aureus protein a based ligands |
| WO2013142349A1 (en) * | 2012-03-23 | 2013-09-26 | University Of Chicago | Compositions and methods related to staphylococcal sbi |
| GB201619965D0 (en) | 2016-11-25 | 2017-01-11 | Univ Of Bath The | Immunogenic compositions comprising sbi protein and uses thereof |
| CA3211083A1 (en) | 2021-03-04 | 2022-09-09 | Kyle BACKMAN | Compositions including sbi adjuvants and methods of use thereof |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SE9704141D0 (sv) * | 1997-11-12 | 1997-11-12 | Sbl Vaccin Ab | New protein and nucleotide sequence, encoding said protein |
| AT410798B (de) * | 2001-01-26 | 2003-07-25 | Cistem Biotechnologies Gmbh | Verfahren zur identifizierung, isolierung und herstellung von antigenen gegen ein spezifisches pathogen |
| ATE542829T1 (de) * | 2001-08-02 | 2012-02-15 | Univ Sheffield | Impfstoff |
| NZ553776A (en) * | 2004-09-22 | 2010-05-28 | Glaxosmithkline Biolog Sa | Immunogenic composition comprising staphylococcal PNAG and Type 5 and/or 8 Capsular polysaccharide or oligosaccharide. |
| GB0526038D0 (en) * | 2005-12-21 | 2006-02-01 | Glaxosmithkline Biolog Sa | Immunogenic composition |
-
2006
- 2006-05-31 GB GB0610776A patent/GB0610776D0/en not_active Ceased
-
2007
- 2007-05-31 CA CA002653668A patent/CA2653668A1/en not_active Abandoned
- 2007-05-31 AU AU2007266813A patent/AU2007266813A1/en not_active Abandoned
- 2007-05-31 EP EP07733038A patent/EP2041165A2/de not_active Withdrawn
- 2007-05-31 WO PCT/GB2007/002022 patent/WO2007138328A2/en not_active Ceased
Non-Patent Citations (1)
| Title |
|---|
| See references of WO2007138328A3 * |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2007138328A3 (en) | 2008-05-08 |
| CA2653668A1 (en) | 2007-12-06 |
| WO2007138328A2 (en) | 2007-12-06 |
| GB0610776D0 (en) | 2006-07-12 |
| AU2007266813A1 (en) | 2007-12-06 |
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