EP2126122A4 - Verfahren und testkit zur bestimmung der mengen an zielsequenzen und nukleotidvariationen darin - Google Patents

Verfahren und testkit zur bestimmung der mengen an zielsequenzen und nukleotidvariationen darin

Info

Publication number
EP2126122A4
EP2126122A4 EP08718527A EP08718527A EP2126122A4 EP 2126122 A4 EP2126122 A4 EP 2126122A4 EP 08718527 A EP08718527 A EP 08718527A EP 08718527 A EP08718527 A EP 08718527A EP 2126122 A4 EP2126122 A4 EP 2126122A4
Authority
EP
European Patent Office
Prior art keywords
quantities
determining
test kit
target sequences
nucleotide variations
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP08718527A
Other languages
English (en)
French (fr)
Other versions
EP2126122A1 (de
Inventor
Hans Soederlund
Jari Rautio
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
VTT Technical Research Centre of Finland Ltd
Original Assignee
VTT Technical Research Centre of Finland Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by VTT Technical Research Centre of Finland Ltd filed Critical VTT Technical Research Centre of Finland Ltd
Publication of EP2126122A1 publication Critical patent/EP2126122A1/de
Publication of EP2126122A4 publication Critical patent/EP2126122A4/de
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP08718527A 2007-02-21 2008-02-20 Verfahren und testkit zur bestimmung der mengen an zielsequenzen und nukleotidvariationen darin Withdrawn EP2126122A4 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI20075124A FI20075124A0 (fi) 2007-02-21 2007-02-21 Menetelmä ja testikitti nukleotidivariaatioiden toteamiseksi
PCT/FI2008/050074 WO2008102057A1 (en) 2007-02-21 2008-02-20 Method and test kit for determining the amounts of target sequences and nucleotide variations therein

Publications (2)

Publication Number Publication Date
EP2126122A1 EP2126122A1 (de) 2009-12-02
EP2126122A4 true EP2126122A4 (de) 2010-11-10

Family

ID=37832273

Family Applications (1)

Application Number Title Priority Date Filing Date
EP08718527A Withdrawn EP2126122A4 (de) 2007-02-21 2008-02-20 Verfahren und testkit zur bestimmung der mengen an zielsequenzen und nukleotidvariationen darin

Country Status (6)

Country Link
EP (1) EP2126122A4 (de)
JP (1) JP2010518846A (de)
AU (1) AU2008217678B9 (de)
CA (1) CA2678693A1 (de)
FI (1) FI20075124A0 (de)
WO (1) WO2008102057A1 (de)

Families Citing this family (61)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9029085B2 (en) 2007-03-07 2015-05-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
JP5738597B2 (ja) 2007-12-21 2015-06-24 プレジデント アンド フェローズ オブ ハーバード カレッジ 核酸の配列決定のためのシステムおよび方法
WO2010033200A2 (en) 2008-09-19 2010-03-25 President And Fellows Of Harvard College Creation of libraries of droplets and related species
EP3150724A1 (de) * 2008-12-19 2017-04-05 President and Fellows of Harvard College Partikelunterstützte nukleinsäuresequenzierung
JP5791621B2 (ja) 2009-10-27 2015-10-07 プレジデント アンド フェローズ オブ ハーバード カレッジ 液滴生成技術
MX352460B (es) 2011-01-11 2017-11-24 Seegene Inc Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto.
JP5852222B2 (ja) 2011-03-29 2016-02-03 シージーン アイエヌシー Ｐｔｏ切断及び延長−依存的切断によるターゲット核酸配列の検出
MX342067B (es) 2011-05-04 2016-09-09 Seegene Inc Detección de secuencias de ácido nucleico objetivo por desdoblamiento e hibridización de oligonucleótido de sonda.
KR20130101952A (ko) 2012-02-02 2013-09-16 주식회사 씨젠 Ｐｔｏ 절단과 연장-의존적 혼성화를 이용한 타겟 핵산서열의 검출
EP2823061B1 (de) * 2012-03-05 2018-02-14 Seegene, Inc. Detektion einer nucleotidvariation einer nucleinsäurezielsequenz durch pto-spaltung und extensionstest
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
AU2013302756C1 (en) 2012-08-14 2018-05-17 10X Genomics, Inc. Microcapsule compositions and methods
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA2894694C (en) 2012-12-14 2023-04-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
KR20200140929A (ko) 2013-02-08 2020-12-16 10엑스 제노믹스, 인크. 폴리뉴클레오티드 바코드 생성
AU2014200958B2 (en) * 2013-02-25 2016-01-14 Seegene, Inc. Detection of nucleotide variation on target nucleic acid sequence
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
WO2015069634A1 (en) 2013-11-08 2015-05-14 President And Fellows Of Harvard College Microparticles, methods for their preparation and use
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
BR112016023625A2 (pt) 2014-04-10 2018-06-26 10X Genomics, Inc. dispositivos fluídicos, sistemas e métodos para encapsular e particionar reagentes, e aplicações dos mesmos
CA2953469A1 (en) 2014-06-26 2015-12-30 10X Genomics, Inc. Analysis of nucleic acid sequences
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
WO2015200893A2 (en) 2014-06-26 2015-12-30 10X Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
KR20170023979A (ko) 2014-06-26 2017-03-06 10엑스 제노믹스, 인크. 핵산 서열 조립을 위한 프로세스 및 시스템
BR112017008877A2 (pt) 2014-10-29 2018-07-03 10X Genomics Inc métodos e composições para sequenciamento de ácido nucleico-alvo
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
JP6769969B2 (ja) 2015-01-12 2020-10-14 １０エックス ジェノミクス， インコーポレイテッド 核酸配列決定ライブラリーを作製するためのプロセス及びシステム、並びにこれらを使用して作製したライブラリー
WO2016115273A1 (en) 2015-01-13 2016-07-21 10X Genomics, Inc. Systems and methods for visualizing structural variation and phasing information
WO2016130578A1 (en) 2015-02-09 2016-08-18 10X Genomics, Inc. Systems and methods for determining structural variation and phasing using variant call data
EP3259356B1 (de) * 2015-02-20 2021-12-01 Rosalind Franklin University of Medicine and Science Antisense-verbindungen mit abzielung auf gene im zusammenhang mit zystischer fibrose
US10525076B2 (en) 2015-02-20 2020-01-07 Rosalind Franklin University Of Medicine And Science Antisense compounds targeting genes associated with cystic fibrosis
EP3262407B1 (de) 2015-02-24 2023-08-30 10X Genomics, Inc. Partitionsverarbeitungsverfahren und -systeme
MX2017010857A (es) 2015-02-24 2017-12-11 10X Genomics Inc Metodos para la cobertura de secuencia de acidos nucleicos seleccionados como diana.
JP2018537414A (ja) 2015-10-13 2018-12-20 プレジデント アンド フェローズ オブ ハーバード カレッジ ゲルマイクロスフェアの作製及び使用のためのシステム及び方法
JP6954899B2 (ja) 2015-12-04 2021-10-27 １０エックス ゲノミクス，インコーポレイテッド 核酸の解析のための方法及び組成物
CN107024583B (zh) * 2016-01-29 2019-03-08 中国科学院苏州纳米技术与纳米仿生研究所 硫黄素t适配体、其筛选方法及应用
WO2017138984A1 (en) 2016-02-11 2017-08-17 10X Genomics, Inc. Systems, methods, and media for de novo assembly of whole genome sequence data
WO2017197343A2 (en) 2016-05-12 2017-11-16 10X Genomics, Inc. Microfluidic on-chip filters
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
EP4029939B1 (de) 2017-01-30 2023-06-28 10X Genomics, Inc. Verfahren und systeme für eine einzelzellbarcodierung auf tröpfchenbasis
EP3625715A4 (de) 2017-05-19 2021-03-17 10X Genomics, Inc. Systeme und verfahren zur analyse von datensätzen
US10400235B2 (en) 2017-05-26 2019-09-03 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
EP3445876B1 (de) 2017-05-26 2023-07-05 10X Genomics, Inc. Einzelzellenanalyse von transposasezugänglichem chromatin
KR102345601B1 (ko) 2017-09-29 2021-12-30 주식회사 씨젠 Pto 절단 및 연장-의존적 연장 분석에 의한 타겟 핵산 서열의 검출
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
CN112262218B (zh) 2018-04-06 2024-11-08 10X基因组学有限公司 用于单细胞处理中的质量控制的系统和方法
CN115428088A (zh) 2020-02-13 2022-12-02 10X基因组学有限公司 用于基因表达和dna染色质可及性的联合交互式可视化的系统和方法
CN117642506A (zh) * 2021-04-07 2024-03-01 马萨诸塞大学 无义密码子的特定寡核苷酸编程的通读
CN114966891B (zh) * 2022-05-18 2026-02-03 中国农业大学 未包裹dna土壤水示踪剂及制备方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1101824A2 (de) * 1999-11-22 2001-05-23 Xiao Bing Wang Detektion von Sequenzvariationen von Nukleinsäuren durch die Analyse veränderter Termination
US20060035228A1 (en) * 2002-07-05 2006-02-16 Hans Soderlund Method and test kit for quantitative determination of polynucleotides in a mixture

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6013431A (en) * 1990-02-16 2000-01-11 Molecular Tool, Inc. Method for determining specific nucleotide variations by primer extension in the presence of mixture of labeled nucleotides and terminators
WO1993004199A2 (en) * 1991-08-20 1993-03-04 Scientific Generics Limited Methods of detecting or quantitating nucleic acids and of producing labelled immobilised nucleic acids
BR9206705A (pt) * 1991-11-01 1995-11-21 Adelaide Children S Hospital Processo de amplificação de fase sólida
EP0636186B1 (de) * 1992-04-03 1998-11-25 The Perkin-Elmer Corporation Proben zusammensetzung und verfahren
EP1164203B1 (de) * 1996-11-06 2007-10-10 Sequenom, Inc. DNA-Diagnostik mittels Massenspektrometrie
AU1623899A (en) * 1997-12-04 1999-06-16 Packard Bioscience Company Methods of using probes for analyzing polynucleotide sequence
US6635419B1 (en) * 1999-02-16 2003-10-21 Applera Corporation Polynucleotide sequencing method
WO2000056937A2 (en) * 1999-03-25 2000-09-28 Hyseq, Inc. Solution-based methods and materials for sequence analysis by hybridization
FI115139B (fi) * 2001-01-10 2005-03-15 Valtion Teknillinen Menetelmä ja testipakkaus solu- tai kudosnäytteissä olevien polynukleotidien määrässä tapahtuvien vaihteluiden kvantitatiiviseen ja/tai vertailevaan arvioimiseen
US20030077584A1 (en) * 2001-08-28 2003-04-24 Mark Kunkel Methods and compositons for bi-directional polymorphism detection
JP3752466B2 (ja) * 2002-04-24 2006-03-08 株式会社日立製作所 遺伝子検査方法
CN1665938B (zh) * 2002-06-28 2012-10-10 普里梅拉蒂斯有限公司 检测序列差异的方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1101824A2 (de) * 1999-11-22 2001-05-23 Xiao Bing Wang Detektion von Sequenzvariationen von Nukleinsäuren durch die Analyse veränderter Termination
US20060035228A1 (en) * 2002-07-05 2006-02-16 Hans Soderlund Method and test kit for quantitative determination of polynucleotides in a mixture

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of WO2008102057A1 *

Also Published As

Publication number Publication date
WO2008102057A9 (en) 2008-10-09
EP2126122A1 (de) 2009-12-02
CA2678693A1 (en) 2008-08-28
AU2008217678B9 (en) 2013-10-03
WO2008102057A1 (en) 2008-08-28
FI20075124A0 (fi) 2007-02-21
JP2010518846A (ja) 2010-06-03
AU2008217678B2 (en) 2013-09-12
AU2008217678A1 (en) 2008-08-28

Similar Documents

Publication Publication Date Title
EP2126122A4 (de) Verfahren und testkit zur bestimmung der mengen an zielsequenzen und nukleotidvariationen darin
EP2041557A4 (de) Verfahren und vorrichtung für verbesserte zielzusammensetzungsbestimmung
EP2232263A4 (de) Verfahren zur bestimmung der ausscheidung von natrium und anderen analyten
EP2271751A4 (de) Erzeugung modifizierter polymerasen für erhöhte genauigkeit bei einzelmolekülsequenzierungen
EP2029741A4 (de) Schnellverfahren zur bestimmung der hemmersensitivität von aus klinischen proben geklonten ns3/4a-proteasesequenzen
EP2330220A4 (de) Verfahren zur quantifizierung oder den nachweis von dna
EP2074827A4 (de) Verfahren zur videoqualitätsbestimmung
EP2051820A4 (de) Verfahren zur verbesserung der haftung von polymermaterialien auf metalloberflächen
EP2183394A4 (de) Verfahren und anwendungen für zielquantifizierung
EP2218005A4 (de) Prüfverfahren für ein neukonfigurierbares prüfgerät
EP2224912A4 (de) Verbesserte zusammensetzungen und verfahren zur freisetzung von nukleinsäuren
EP2021958A4 (de) Verwendungsbezogene indikatoren zur beurteilung der auswirkung von wissenschaftlichen arbeiten aufbau und verfahren
EP2207037A4 (de) Krebsnachweisverfahren
EP2332021A4 (de) System und verfahren für parkzeitschätzungen
EP2035440A4 (de) Primer- und sondensequenzen für den nachweis von chlamydia trachomatis
EP2327799A4 (de) Verfahren zur quantifizierung oder den nachweis von dna
EP2218791A4 (de) Verfahren und kit für die zubereitung einer probe zur nukleinsäureamplifikation
EP2246446A4 (de) Verfahren zur quantifizierung einer zielsubstanz
EP2201355A4 (de) Verfahren zur identifizierung elektrochemischer teststreifen
EP2081021A4 (de) Hämoglobinbestimmungsverfahren
EP2132568A4 (de) Verfahren zur bestimmung des skolioserisikos
FR2907794B1 (fr) Methode de detection d'oligonucleotides courts
EP2109882A4 (de) Verfahren zur bildung von öffnungen in gewähltem material
EP2206793A4 (de) Verfahren und kit zur entdeckung/quantifizierung einer ziel-rna
EP2237036A4 (de) Detektionsverfahren und bestimmungsverfahren für ein detektionsziel

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20090918

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MT NL NO PL PT RO SE SI SK TR

DAX Request for extension of the european patent (deleted)
A4 Supplementary search report drawn up and despatched

Effective date: 20101007

17Q First examination report despatched

Effective date: 20110627

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20130504