EP2300613A4 - Mutants d'adn polymérases thermiquement stables modifiés chimiquement - Google Patents

Mutants d'adn polymérases thermiquement stables modifiés chimiquement

Info

Publication number
EP2300613A4
EP2300613A4 EP09767773A EP09767773A EP2300613A4 EP 2300613 A4 EP2300613 A4 EP 2300613A4 EP 09767773 A EP09767773 A EP 09767773A EP 09767773 A EP09767773 A EP 09767773A EP 2300613 A4 EP2300613 A4 EP 2300613A4
Authority
EP
European Patent Office
Prior art keywords
mutated
chemically modified
thermally stable
dna polymerases
stable dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP09767773A
Other languages
German (de)
English (en)
Other versions
EP2300613A2 (fr
Inventor
Lei Xi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Corp
Original Assignee
Life Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Corp filed Critical Life Technologies Corp
Publication of EP2300613A2 publication Critical patent/EP2300613A2/fr
Publication of EP2300613A4 publication Critical patent/EP2300613A4/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP09767773A 2008-06-18 2009-06-18 Mutants d'adn polymérases thermiquement stables modifiés chimiquement Withdrawn EP2300613A4 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US7375408P 2008-06-18 2008-06-18
PCT/US2009/047862 WO2009155464A2 (fr) 2008-06-18 2009-06-18 Mutants d'adn polymérases thermiquement stables modifiés chimiquement

Publications (2)

Publication Number Publication Date
EP2300613A2 EP2300613A2 (fr) 2011-03-30
EP2300613A4 true EP2300613A4 (fr) 2011-11-09

Family

ID=41434700

Family Applications (1)

Application Number Title Priority Date Filing Date
EP09767773A Withdrawn EP2300613A4 (fr) 2008-06-18 2009-06-18 Mutants d'adn polymérases thermiquement stables modifiés chimiquement

Country Status (2)

Country Link
EP (1) EP2300613A4 (fr)
WO (1) WO2009155464A2 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010062777A2 (fr) 2008-11-03 2010-06-03 Kapabiosystems Adn polymérases de type a modifiées
CN102834510A (zh) * 2010-02-05 2012-12-19 新英格兰生物实验室公司 高保真性限制性内切核酸酶
US9315787B2 (en) 2011-01-14 2016-04-19 Kapa Biosystems, Inc. Modified DNA polymerases for improved amplification
US20140363875A1 (en) * 2011-07-12 2014-12-11 Kyushu University, National University Corporation Novel dna polymerase
WO2013160316A1 (fr) * 2012-04-23 2013-10-31 Dsm Ip Assets B.V. Procédé d'expression de polypeptides
DE102012216193B4 (de) 2012-09-12 2020-07-30 Continental Automotive Gmbh Verfahren und Vorrichtung zur Bedienung einer Kraftfahrzeugkomponente mittels Gesten
CN114835795A (zh) * 2015-11-16 2022-08-02 Ubi蛋白公司 用于延长蛋白质半衰期的方法
WO2018203582A1 (fr) * 2017-05-05 2018-11-08 주식회사 유비프로틴 Procédé pour prolonger la demi-vie d'une protéine
WO2021242041A1 (fr) * 2020-05-29 2021-12-02 (주)제노텍 Variant d'adn polymérase permettant une discrimination améliorée de variants génétiques
CN115011579B (zh) * 2022-04-28 2024-02-02 北京丹大生物技术有限公司 一种Taq DNA聚合酶突变体及其制备方法和应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002022869A2 (fr) * 2000-09-13 2002-03-21 Medical Research Council Procede d'evolution dirigee

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5773258A (en) * 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
KR100624490B1 (ko) * 2004-05-27 2006-09-20 바이오퀘스트(주) 화학변형 열안정성 ｄｎａ 중합효소

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002022869A2 (fr) * 2000-09-13 2002-03-21 Medical Research Council Procede d'evolution dirigee

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
GHADESSY FARID J ET AL: "Directed evolution of polymerase function by compartmentalized self-replication", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, WASHINGTON, DC; US, vol. 98, no. 8, 10 April 2001 (2001-04-10), pages 4552 - 4557, XP002189673, ISSN: 0027-8424, DOI: 10.1073/PNAS.071052198 *

Also Published As

Publication number Publication date
EP2300613A2 (fr) 2011-03-30
WO2009155464A3 (fr) 2010-05-06
WO2009155464A2 (fr) 2009-12-23

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