EP2334802A4 - Procédés de génération de bibliothèques spécifiques de gènes - Google Patents

Procédés de génération de bibliothèques spécifiques de gènes

Info

Publication number
EP2334802A4
EP2334802A4 EP09813548A EP09813548A EP2334802A4 EP 2334802 A4 EP2334802 A4 EP 2334802A4 EP 09813548 A EP09813548 A EP 09813548A EP 09813548 A EP09813548 A EP 09813548A EP 2334802 A4 EP2334802 A4 EP 2334802A4
Authority
EP
European Patent Office
Prior art keywords
methods
gene specific
specific libraries
generating gene
generating
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP09813548A
Other languages
German (de)
English (en)
Other versions
EP2334802A1 (fr
Inventor
Christopher K Raymond
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Corp
Original Assignee
Life Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Corp filed Critical Life Technologies Corp
Publication of EP2334802A1 publication Critical patent/EP2334802A1/fr
Publication of EP2334802A4 publication Critical patent/EP2334802A4/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP09813548A 2008-09-09 2009-09-09 Procédés de génération de bibliothèques spécifiques de gènes Withdrawn EP2334802A4 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US9557908P 2008-09-09 2008-09-09
PCT/US2009/056380 WO2010030683A1 (fr) 2008-09-09 2009-09-09 Procédés de génération de bibliothèques spécifiques de gènes

Publications (2)

Publication Number Publication Date
EP2334802A1 EP2334802A1 (fr) 2011-06-22
EP2334802A4 true EP2334802A4 (fr) 2012-01-25

Family

ID=42005453

Family Applications (1)

Application Number Title Priority Date Filing Date
EP09813548A Withdrawn EP2334802A4 (fr) 2008-09-09 2009-09-09 Procédés de génération de bibliothèques spécifiques de gènes

Country Status (3)

Country Link
EP (1) EP2334802A4 (fr)
CN (1) CN102203273A (fr)
WO (1) WO2010030683A1 (fr)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2391732B1 (fr) 2009-01-30 2015-05-27 Oxford Nanopore Technologies Limited Methodes qui utilisent des adaptateurs pour la construction d'acides nucleiques pour le sequençage transmembranaire
EP2580378A4 (fr) * 2010-06-08 2014-01-01 Nugen Technologies Inc Méthodes et composition de séquençage multiplex
CN102409043B (zh) * 2010-09-21 2013-12-04 深圳华大基因科技服务有限公司 高通量低成本Fosmid文库构建的方法及其所使用标签和标签接头
CN102409408B (zh) * 2010-09-21 2013-08-07 深圳华大基因科技服务有限公司 一种利用微量基因组dna进行全基因组甲基化位点精确检测的方法
WO2012175013A1 (fr) * 2011-06-24 2012-12-27 深圳华大基因科技有限公司 Système et méthode de diagnostic d'un corps humain ayant un état anormal
WO2013075313A1 (fr) * 2011-11-24 2013-05-30 深圳华大基因科技有限公司 Sonde de détection du mode d'intégration d'un virus dans un échantillon d'essai et procédé de préparation et d'utilisation associé
JP6298404B2 (ja) * 2011-07-25 2018-03-20 オックスフォード ナノポール テクノロジーズ リミテッド 膜貫通ポアを用いる二重鎖ポリヌクレオチド配列決定のためのヘアピンループ方法
WO2013059746A1 (fr) 2011-10-19 2013-04-25 Nugen Technologies, Inc. Compositions et procédés pour l'amplification et le séquençage directionnels d'acide nucléique
EP2807292B1 (fr) 2012-01-26 2019-05-22 Tecan Genomics, Inc. Compositions et procédés pour l'enrichissement en séquence d'acide nucléique ciblée et la génération d'une banque à efficacité élevée
CA2877094A1 (fr) 2012-06-18 2013-12-27 Nugen Technologies, Inc. Compositions et procedes pour la selection negative de sequences d'acide nucleique indesirable
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
US11155860B2 (en) 2012-07-19 2021-10-26 Oxford Nanopore Technologies Ltd. SSB method
WO2014071361A1 (fr) * 2012-11-05 2014-05-08 Rubicon Genomics Marquage par code-barre d'acides nucléiques
CA2901545C (fr) 2013-03-08 2019-10-08 Oxford Nanopore Technologies Limited Utilisation d'elements espaceurs dans un acide nucleique pour controler le mouvement d'un helicase
US9822408B2 (en) 2013-03-15 2017-11-21 Nugen Technologies, Inc. Sequential sequencing
WO2015073711A1 (fr) 2013-11-13 2015-05-21 Nugen Technologies, Inc. Compositions et procédés pour l'identification d'une lecture de séquençage en double
CN106029962A (zh) * 2014-02-11 2016-10-12 豪夫迈·罗氏有限公司 靶向测序和uid过滤
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
US9745614B2 (en) 2014-02-28 2017-08-29 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
CN106604994B (zh) * 2014-06-23 2021-12-14 通用医疗公司 通过测序评估的DSBs的全基因组无偏鉴定(GUIDE-Seq)
CA2957633A1 (fr) 2014-08-06 2016-02-11 Nugen Technologies, Inc. Mesures numeriques a partir de sequencage cible
DK3192869T3 (da) * 2014-09-12 2019-05-20 Mgi Tech Co Ltd Isoleret oligonukleotid og anvendelse deraf i nukleinsyresekvensering
CA2968552A1 (fr) * 2014-12-02 2016-06-09 Tribiotica Llc Procedes et kits pour des applications theranostiques
US9857328B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Chemically-sensitive field effect transistors, systems and methods for manufacturing and using the same
US10006910B2 (en) 2014-12-18 2018-06-26 Agilome, Inc. Chemically-sensitive field effect transistors, systems, and methods for manufacturing and using the same
US9618474B2 (en) 2014-12-18 2017-04-11 Edico Genome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
CA2971589C (fr) 2014-12-18 2021-09-28 Edico Genome Corporation Transistor a effet de champ chimiquement sensible
US9859394B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US10020300B2 (en) 2014-12-18 2018-07-10 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US10640752B2 (en) * 2014-12-22 2020-05-05 Veterinærinstituttet Salmon gill poxvirus
CN104946639B (zh) * 2015-07-01 2017-10-31 益善生物技术股份有限公司 构建基因突变测序文库的引物和方法以及试剂盒
JP6913089B2 (ja) * 2015-11-11 2021-08-04 レゾリューション バイオサイエンス， インコーポレイテッド Ｄｎａライブラリーの高効率構築
WO2017117440A1 (fr) * 2015-12-30 2017-07-06 Bio-Rad Laboratories, Inc. Préparation de banque de pcr séparée en gouttelettes
WO2017201081A1 (fr) 2016-05-16 2017-11-23 Agilome, Inc. Dispositifs à fet au graphène, systèmes et leurs méthodes d'utilisation pour le séquençage d'acides nucléiques
GB201609220D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
EP3532635B1 (fr) * 2016-10-31 2021-06-09 F. Hoffmann-La Roche AG Construction de bibliothèque circulaire à code-barres pour l'identification de produits chimériques
IL266681B2 (en) * 2016-11-18 2025-01-01 Twist Bioscience Corp Polynucleotide libraries with controlled stoichiometry and their synthesis
US12492430B2 (en) 2017-04-11 2025-12-09 Tecan Genomics, Inc. Library quantitation and qualification
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
CN109750092B (zh) * 2017-11-03 2022-12-06 北京贝瑞和康生物技术有限公司 一种靶向富集高gc含量目标dna的方法和试剂盒
CN108004301B (zh) * 2017-12-15 2022-02-22 格诺思博生物科技南通有限公司 基因目标区域富集方法及建库试剂盒
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method
EP3851542A1 (fr) 2020-01-20 2021-07-21 Tecan Genomics, Inc. Épuisement de séquences non informatives abondantes
WO2021155374A2 (fr) 2020-01-31 2021-08-05 Avida Biomed, Inc. Systèmes et procédés de capture ciblée d'acides nucléiques
US12059674B2 (en) 2020-02-03 2024-08-13 Tecan Genomics, Inc. Reagent storage system

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1184466A2 (fr) * 2000-08-26 2002-03-06 Affymetrix, Inc. Enrichissiment et amplification des cibles d'acide nucleique pour l'analyse dans un réseau
WO2002083943A2 (fr) * 2001-04-12 2002-10-24 Epigenomics Ag Procede a microreseau permettant d'enrichir des fragments d'adn provenant de melanges complexes
WO2007057652A1 (fr) * 2005-11-15 2007-05-24 Solexa Limited Methode d'enrichissement de cible

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5681726A (en) * 1988-09-19 1997-10-28 Stratagene Method of double stranded DNA synthesis
WO2002034949A2 (fr) * 2000-10-27 2002-05-02 Molecular Staging Inc. Procédés pour identifier des gènes associés à des maladies ou des phénotypes spécifiques
GB0114853D0 (en) * 2001-06-18 2001-08-08 Medical Res Council Happier Mapping
WO2004009796A2 (fr) * 2002-07-24 2004-01-29 Immusol Incorporated Systeme a un seul promoteur pour la production de cassettes d'expression d'arnsi et genotheque d'expression reposant sur l'utilisation d'un lieur en epingle a cheveux d'amorce de polymerase
WO2006007569A2 (fr) * 2004-07-01 2006-01-19 Somagenics, Inc. Procedes de preparation de bibliotheques d'oligonucleotides propres aux genes et utilisations associees
WO2007018601A1 (fr) * 2005-08-02 2007-02-15 Rubicon Genomics, Inc. Compositions et methodes de traitement et d'amplification d'adn consistant a utiliser plusieurs enzymes dans une seule reaction

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1184466A2 (fr) * 2000-08-26 2002-03-06 Affymetrix, Inc. Enrichissiment et amplification des cibles d'acide nucleique pour l'analyse dans un réseau
WO2002083943A2 (fr) * 2001-04-12 2002-10-24 Epigenomics Ag Procede a microreseau permettant d'enrichir des fragments d'adn provenant de melanges complexes
WO2007057652A1 (fr) * 2005-11-15 2007-05-24 Solexa Limited Methode d'enrichissement de cible

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
JUDY ST. JOHN ET AL: "Rapid capture of DNA targets", BIOTECHNIQUES, vol. 44, no. 2, 1 February 2008 (2008-02-01), pages 259 - 264, XP055014746, ISSN: 0736-6205, DOI: 10.2144/000112633 *
OKOU DAVID T ET AL: "Microarray-based genomic selection for high-throughput resequencing", NATURE METHODS, NATURE PUBLISHING GROUP, GB, vol. 4, no. 11, 1 October 2007 (2007-10-01), pages 907 - 909, XP002528498, ISSN: 1548-7091, DOI: 10.1038/NMETH1109 *
See also references of WO2010030683A1 *
UNN HILDE REFSETH ET AL: "HYBRIDIZATION CAPTURE OF MICROSATELLITES DIRECTLY FROM GENOMIC DNA", ELECTROPHORESIS, WILEY INTERSCIENCE, DE, vol. 18, no. 9, 1 January 1997 (1997-01-01), pages 1519 - 1523, XP002952426, ISSN: 0173-0835, DOI: 10.1002/ELPS.1150180905 *

Also Published As

Publication number Publication date
CN102203273A (zh) 2011-09-28
EP2334802A1 (fr) 2011-06-22
WO2010030683A1 (fr) 2010-03-18

Similar Documents

Publication Publication Date Title
EP2334802A4 (fr) Procédés de génération de bibliothèques spécifiques de gènes
EP2126087A4 (fr) Procedes de production de pharmacotheques, et leurs utilisations
EG25742A (en) Photobioreactor
SG10202000728UA (en) Methods of reducing eosinophil levels
IL211551A0 (en) Improved method of rna display
EP2271952A4 (fr) Visualisation de caractéristiques géologiques à l'aide de représentations de données leur appartenant
IL211758A0 (en) Authorization of server operations
IL212570A0 (en) Composiitons and methods for inhibiting expression of factor vii genes
IL209488A0 (en) Inhibitors of akt activity
ZA201102604B (en) Alkylcyclohexylethers of dihydrotetraazabenzoazulenes
ZA201305274B (en) Data analysis of dna sequences
IL208237A0 (en) Expression-linked gene discovery
PL2133419T3 (pl) Zastosowania beta-dipeptydów cyjanoficyny
EP2303852A4 (fr) Inhibiteurs d activité akt
EP2306825A4 (fr) Inhibiteurs de l'activité de l'akt
GB0811500D0 (en) Method of determining DNA copy number
EP2118297A4 (fr) Génération de molécules d'acide nucléique
GB0718542D0 (en) Targeted modulation of gene expression
GB0807321D0 (en) Analysis of nucleic acids
GB0817384D0 (en) Method of providing location data
EP2303277A4 (fr) Inhibiteurs de l'activité de l'akt
GB0701722D0 (en) Inhibition of gene expression
SI3309253T1 (sl) Geni za biosintezo tetraciklinskih spojin in uporabe le-teh
EP2303017A4 (fr) Inhibiteurs d activité akt
EP2252701A4 (fr) Procédés d'identification d'organisme

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20110408

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR

AX Request for extension of the european patent

Extension state: AL BA RS

DAX Request for extension of the european patent (deleted)
A4 Supplementary search report drawn up and despatched

Effective date: 20111223

RIC1 Information provided on ipc code assigned before grant

Ipc: C40B 50/06 20060101ALI20111219BHEP

Ipc: C40B 40/08 20060101ALI20111219BHEP

Ipc: C40B 40/06 20060101ALI20111219BHEP

Ipc: C12N 15/10 20060101ALI20111219BHEP

Ipc: C12N 15/66 20060101ALI20111219BHEP

Ipc: C12Q 1/68 20060101AFI20111219BHEP

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION HAS BEEN WITHDRAWN

18W Application withdrawn

Effective date: 20120703