EP2356233A2 - Procédé de récolte de cellules et/ou de fragments cellulaires de la muqueuse buccale - Google Patents

Procédé de récolte de cellules et/ou de fragments cellulaires de la muqueuse buccale

Info

Publication number
EP2356233A2
EP2356233A2 EP09801139A EP09801139A EP2356233A2 EP 2356233 A2 EP2356233 A2 EP 2356233A2 EP 09801139 A EP09801139 A EP 09801139A EP 09801139 A EP09801139 A EP 09801139A EP 2356233 A2 EP2356233 A2 EP 2356233A2
Authority
EP
European Patent Office
Prior art keywords
chewing
dna
cells
rna
gum
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP09801139A
Other languages
German (de)
English (en)
Inventor
Hanns-Christian Tillmann
Wolfgang Greb
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EUCRO European Contract Research GmbH and Co KG
Original Assignee
EUCRO European Contract Research GmbH and Co KG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE102008057257A external-priority patent/DE102008057257A1/de
Priority claimed from DE102009016075A external-priority patent/DE102009016075A1/de
Application filed by EUCRO European Contract Research GmbH and Co KG filed Critical EUCRO European Contract Research GmbH and Co KG
Publication of EP2356233A2 publication Critical patent/EP2356233A2/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Definitions

  • the cells and / or cell fragments obtained are subjected to DNA extraction / RNA extraction in a subsequent method step.
  • the extraction can be carried out in a manner known per se by first breaking up the isolated cells and separating and purifying the DNA and / or RNA from the cell debris and other substances.
  • the disruption of the cells can be carried out mechanically, by heating, by enzymatic dissolution or by chemical dissolution by means of surfactants.
  • the purification of the DNA or the RNA is also A is well known to the person skilled in the art and can be effected, for example, by precipitation with alcohol, phenol / chloroform or salt or saline solution, and separation by chromatography is also possible.
  • the chewing gland contains specific primers (oligonucleotides) by means of which DNA and / or RNA can be selectively identified and recovered. This is followed by amplification of the DNA and / or RNA by known methods (e.g., PCR).
  • human DNA or RNA may be derived from non-human, e.g. microbial; Distinguish DNA / RNA and selectively win with the chewing gum.
  • primers can be used, as described in Labuhn et al. (The International Journal of Biological Markers, 2006).

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Confectionery (AREA)

Abstract

L'invention porte sur un procédé de récolte de cellules et/ou de fragments cellulaires de la muqueuse buccale de patients (sujet de test/homme), suivant lequel l'homme ou le patient mastique une masse à mastiquer non assimilable, insoluble dans l'eau, à l'occasion de quoi les cellules de la muqueuse buccale et/ou l'ADN et/ou l'ARN et/ou leurs fragments sont absorbés par la masse à mastiquer pendant le processus de mastication et les cellules absorbées et/ou les fragments cellulaires absorbés peuvent être séparés de la masse de mastication après la fin du processus de mastication. Les cellules/fragments cellulaires peuvent être utilisés pour l'obtention d'ADN, de constituants cellulaires, de cellules humaines, de bactéries et de virus et dans d'autres buts analytiques.
EP09801139A 2008-11-13 2009-11-13 Procédé de récolte de cellules et/ou de fragments cellulaires de la muqueuse buccale Withdrawn EP2356233A2 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102008057257A DE102008057257A1 (de) 2008-11-13 2008-11-13 Verfahren zum Sammeln von Mundschleimhautzellen und/oder -zellfragmenten
DE102009016075A DE102009016075A1 (de) 2009-04-03 2009-04-03 Verfahren zum Sammeln von Mundschleimhautzellen und/oder -zellfragmenten bzw. DNA
PCT/DE2009/001594 WO2010054639A2 (fr) 2008-11-13 2009-11-13 Procédé de récolte de cellules et/ou de fragments cellulaires de la muqueuse buccale

Publications (1)

Publication Number Publication Date
EP2356233A2 true EP2356233A2 (fr) 2011-08-17

Family

ID=42170446

Family Applications (1)

Application Number Title Priority Date Filing Date
EP09801139A Withdrawn EP2356233A2 (fr) 2008-11-13 2009-11-13 Procédé de récolte de cellules et/ou de fragments cellulaires de la muqueuse buccale

Country Status (2)

Country Link
EP (1) EP2356233A2 (fr)
WO (1) WO2010054639A2 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105189745B (zh) * 2013-03-14 2021-10-29 Wm.雷格利 Jr.公司 可延展聚合物内的核酸的检测和定量方法
EP3092313B1 (fr) * 2014-01-10 2018-07-18 Wm. Wrigley Jr. Company Méthodes de détection et de quantification de bactéries contenues sur ou dans de la gomme à mâcher
CN115515429A (zh) * 2020-05-04 2022-12-23 Wm.雷格利 Jr.公司 可咀嚼基质及其用于收集唾液样品和感染检测的用途

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001079554A1 (fr) * 2000-04-13 2001-10-25 Georgetown University Diagnostic genetique des reactions indesirables aux medicaments associees a une prolongation de l'intervalle qt

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3632133A1 (de) 1986-09-22 1988-03-31 Faull Elmar Dr Kaugummi fuer diagnosezwecke
EP1181010A4 (fr) 1999-04-06 2003-03-26 Wrigley W M Jun Co Formulations pharmaceutiques de gomme a macher

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001079554A1 (fr) * 2000-04-13 2001-10-25 Georgetown University Diagnostic genetique des reactions indesirables aux medicaments associees a une prolongation de l'intervalle qt

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JOHN F CARLQUIST ET AL: "Genotypes of the cytochrome p450 isoform, CYP2C9, and the vitamin K epoxide reductase complex subunit 1 conjointly determine stable warfarin dose: a prospective study", JOURNAL OF THROMBOSIS AND THROMBOLYSIS, KLUWER ACADEMIC PUBLISHERS, BO, vol. 22, no. 3, 17 November 2006 (2006-11-17), pages 191 - 197, XP019454829, ISSN: 1573-742X, DOI: 10.1007/S11239-006-9030-7 *
See also references of WO2010054639A2 *

Also Published As

Publication number Publication date
WO2010054639A4 (fr) 2010-12-16
WO2010054639A2 (fr) 2010-05-20
WO2010054639A3 (fr) 2010-10-21

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