EP2405959A4 - METHODS, APPARATUSES AND KITS FOR INTRODUCING GENETIC MATERIAL INTO LIVING CELLS - Google Patents

METHODS, APPARATUSES AND KITS FOR INTRODUCING GENETIC MATERIAL INTO LIVING CELLS

Info

Publication number
EP2405959A4
EP2405959A4 EP10751463.0A EP10751463A EP2405959A4 EP 2405959 A4 EP2405959 A4 EP 2405959A4 EP 10751463 A EP10751463 A EP 10751463A EP 2405959 A4 EP2405959 A4 EP 2405959A4
Authority
EP
European Patent Office
Prior art keywords
kits
apparatuses
methods
genetic material
living cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP10751463.0A
Other languages
German (de)
French (fr)
Other versions
EP2405959A1 (en
Inventor
Thomas Diefenbach
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tufts University
Original Assignee
Tufts University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tufts University filed Critical Tufts University
Publication of EP2405959A1 publication Critical patent/EP2405959A1/en
Publication of EP2405959A4 publication Critical patent/EP2405959A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/89—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli

Landscapes

  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Sustainable Development (AREA)
  • Mechanical Engineering (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
EP10751463.0A 2009-03-13 2010-03-12 METHODS, APPARATUSES AND KITS FOR INTRODUCING GENETIC MATERIAL INTO LIVING CELLS Withdrawn EP2405959A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US15985609P 2009-03-13 2009-03-13
PCT/US2010/027104 WO2010105135A1 (en) 2009-03-13 2010-03-12 Methods, apparatuses, and kits for introducing genetic material into living cells

Publications (2)

Publication Number Publication Date
EP2405959A1 EP2405959A1 (en) 2012-01-18
EP2405959A4 true EP2405959A4 (en) 2013-10-16

Family

ID=42728810

Family Applications (1)

Application Number Title Priority Date Filing Date
EP10751463.0A Withdrawn EP2405959A4 (en) 2009-03-13 2010-03-12 METHODS, APPARATUSES AND KITS FOR INTRODUCING GENETIC MATERIAL INTO LIVING CELLS

Country Status (2)

Country Link
EP (1) EP2405959A4 (en)
WO (1) WO2010105135A1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9017991B2 (en) * 2009-03-13 2015-04-28 Tufts University Methods tip assemblies and kits for introducing material into cells
CN107058101B (en) 2011-10-17 2021-06-01 麻省理工学院 intracellular delivery
EP3033184B1 (en) 2013-08-16 2021-03-31 Massachusetts Institute of Technology Selective delivery of material to cells
JP7523203B2 (en) 2014-10-31 2024-07-26 マサチューセッツ インスティテュート オブ テクノロジー Delivery of biomolecules to immune cells
WO2016077761A1 (en) 2014-11-14 2016-05-19 Massachusetts Institute Of Technology Disruption and field enabled delivery of compounds and compositions into cells
CN107250373A (en) 2015-01-12 2017-10-13 麻省理工学院 Gene editing by microfluidic delivery
SG10201912910PA (en) 2015-07-09 2020-02-27 Massachusetts Inst Technology Delivery of materials to anucleate cells

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5922687A (en) * 1995-05-04 1999-07-13 Board Of Trustees Of The Leland Stanford Junior University Intracellular delivery of nucleic acids using pressure
EP0998945A1 (en) * 1998-09-30 2000-05-10 Transgene S.A. Use of magnesium (Mg2+) for the enhancement of gene delivery in gene therapy
WO2003061831A2 (en) * 2002-01-25 2003-07-31 Dormann Joerg Martin Sampling device and method for the simultaneous, quantifiable determination of infectious germs
DE10361917A1 (en) * 2003-12-22 2005-07-21 Novosom Ag In vitro transfection of cells, useful e.g. in animal tests and for human applications, comprises centrifuging the cells with active agents, preferably packaged in liposomes
US20070077306A1 (en) * 2003-12-26 2007-04-05 Toshihiro Akaike Apatite particle, method of producing the same, apatite particle-gene complex, and method of gene transfection
DE102007004856A1 (en) * 2007-01-31 2008-08-07 Universität Wien Pipette device, manipulation device and method for manipulating biological cells

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6982063B2 (en) * 2001-05-25 2006-01-03 Matrix Technologies Corp Automated pipetting system

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5922687A (en) * 1995-05-04 1999-07-13 Board Of Trustees Of The Leland Stanford Junior University Intracellular delivery of nucleic acids using pressure
EP0998945A1 (en) * 1998-09-30 2000-05-10 Transgene S.A. Use of magnesium (Mg2+) for the enhancement of gene delivery in gene therapy
WO2003061831A2 (en) * 2002-01-25 2003-07-31 Dormann Joerg Martin Sampling device and method for the simultaneous, quantifiable determination of infectious germs
DE10361917A1 (en) * 2003-12-22 2005-07-21 Novosom Ag In vitro transfection of cells, useful e.g. in animal tests and for human applications, comprises centrifuging the cells with active agents, preferably packaged in liposomes
US20070077306A1 (en) * 2003-12-26 2007-04-05 Toshihiro Akaike Apatite particle, method of producing the same, apatite particle-gene complex, and method of gene transfection
DE102007004856A1 (en) * 2007-01-31 2008-08-07 Universität Wien Pipette device, manipulation device and method for manipulating biological cells

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of WO2010105135A1 *

Also Published As

Publication number Publication date
EP2405959A1 (en) 2012-01-18
WO2010105135A1 (en) 2010-09-16

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