EP2405959A4 - Verfahren, vorrichtungen und kits zur einführung von genetischem material in lebende zellen - Google Patents

Verfahren, vorrichtungen und kits zur einführung von genetischem material in lebende zellen

Info

Publication number
EP2405959A4
EP2405959A4 EP10751463.0A EP10751463A EP2405959A4 EP 2405959 A4 EP2405959 A4 EP 2405959A4 EP 10751463 A EP10751463 A EP 10751463A EP 2405959 A4 EP2405959 A4 EP 2405959A4
Authority
EP
European Patent Office
Prior art keywords
kits
apparatuses
methods
genetic material
living cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP10751463.0A
Other languages
English (en)
French (fr)
Other versions
EP2405959A1 (de
Inventor
Thomas Diefenbach
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tufts University
Original Assignee
Tufts University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tufts University filed Critical Tufts University
Publication of EP2405959A1 publication Critical patent/EP2405959A1/de
Publication of EP2405959A4 publication Critical patent/EP2405959A4/de
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/89—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli

Landscapes

  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Sustainable Development (AREA)
  • Mechanical Engineering (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
EP10751463.0A 2009-03-13 2010-03-12 Verfahren, vorrichtungen und kits zur einführung von genetischem material in lebende zellen Withdrawn EP2405959A4 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US15985609P 2009-03-13 2009-03-13
PCT/US2010/027104 WO2010105135A1 (en) 2009-03-13 2010-03-12 Methods, apparatuses, and kits for introducing genetic material into living cells

Publications (2)

Publication Number Publication Date
EP2405959A1 EP2405959A1 (de) 2012-01-18
EP2405959A4 true EP2405959A4 (de) 2013-10-16

Family

ID=42728810

Family Applications (1)

Application Number Title Priority Date Filing Date
EP10751463.0A Withdrawn EP2405959A4 (de) 2009-03-13 2010-03-12 Verfahren, vorrichtungen und kits zur einführung von genetischem material in lebende zellen

Country Status (2)

Country Link
EP (1) EP2405959A4 (de)
WO (1) WO2010105135A1 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9017991B2 (en) * 2009-03-13 2015-04-28 Tufts University Methods tip assemblies and kits for introducing material into cells
CN107058101B (zh) 2011-10-17 2021-06-01 麻省理工学院 细胞内传递
EP3033184B1 (de) 2013-08-16 2021-03-31 Massachusetts Institute of Technology Selektive freisetzung von material an zellen
JP7523203B2 (ja) 2014-10-31 2024-07-26 マサチューセッツ インスティテュート オブ テクノロジー 生体分子の免疫細胞への送達
WO2016077761A1 (en) 2014-11-14 2016-05-19 Massachusetts Institute Of Technology Disruption and field enabled delivery of compounds and compositions into cells
CN107250373A (zh) 2015-01-12 2017-10-13 麻省理工学院 通过微流体递送实现的基因编辑
SG10201912910PA (en) 2015-07-09 2020-02-27 Massachusetts Inst Technology Delivery of materials to anucleate cells

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5922687A (en) * 1995-05-04 1999-07-13 Board Of Trustees Of The Leland Stanford Junior University Intracellular delivery of nucleic acids using pressure
EP0998945A1 (de) * 1998-09-30 2000-05-10 Transgene S.A. Verwendung von Magnesium (Mg2+) zur Erhöhung der Gen-Verabreichung bei Gentherapie
WO2003061831A2 (de) * 2002-01-25 2003-07-31 Dormann Joerg Martin Probennahmevorrichtung und verfahren zur simultanen, quantifizierbaren bestimmung von infektologisch relevanten keimen
DE10361917A1 (de) * 2003-12-22 2005-07-21 Novosom Ag Verfahren zur Transfektion von Zellen
US20070077306A1 (en) * 2003-12-26 2007-04-05 Toshihiro Akaike Apatite particle, method of producing the same, apatite particle-gene complex, and method of gene transfection
DE102007004856A1 (de) * 2007-01-31 2008-08-07 Universität Wien Pipetteneinrichtung, Manipulationseinrichtung und Verfahren zur Manipulation biologischer Zellen

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6982063B2 (en) * 2001-05-25 2006-01-03 Matrix Technologies Corp Automated pipetting system

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5922687A (en) * 1995-05-04 1999-07-13 Board Of Trustees Of The Leland Stanford Junior University Intracellular delivery of nucleic acids using pressure
EP0998945A1 (de) * 1998-09-30 2000-05-10 Transgene S.A. Verwendung von Magnesium (Mg2+) zur Erhöhung der Gen-Verabreichung bei Gentherapie
WO2003061831A2 (de) * 2002-01-25 2003-07-31 Dormann Joerg Martin Probennahmevorrichtung und verfahren zur simultanen, quantifizierbaren bestimmung von infektologisch relevanten keimen
DE10361917A1 (de) * 2003-12-22 2005-07-21 Novosom Ag Verfahren zur Transfektion von Zellen
US20070077306A1 (en) * 2003-12-26 2007-04-05 Toshihiro Akaike Apatite particle, method of producing the same, apatite particle-gene complex, and method of gene transfection
DE102007004856A1 (de) * 2007-01-31 2008-08-07 Universität Wien Pipetteneinrichtung, Manipulationseinrichtung und Verfahren zur Manipulation biologischer Zellen

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of WO2010105135A1 *

Also Published As

Publication number Publication date
EP2405959A1 (de) 2012-01-18
WO2010105135A1 (en) 2010-09-16

Similar Documents

Publication Publication Date Title
EP2510086A4 (de) Verbesserte verfahren für zellkulturen für adoptive zelltherapien
EP2436075A4 (de) Vorrichtung und verfahren zur herbeiführung elektrochemischer reaktionen
EP2480270A4 (de) Verfahren und vorrichtung zur einführung von zellen an einer gewebestelle
EP2401696A4 (de) Sägerzelllinienmodelle und entsprechende verfahren
EP2421956A4 (de) Zusammensetzungen und verfahren zur gewinnung oder kultivierung pluripotenter zellen
EP2670851A4 (de) Systeme, endstücke, verfahren und kits zur einführung von materialien in zellen
EP2401361A4 (de) Verfahren und zusammensetzungen für stammzellkulturen
EP2744905A4 (de) Verfahren und zusammensetzungen zum einführen einer exogenen dsrna in pflanzenzellen
EP2456856A4 (de) Verfahren und zusammensetzungen zur verbesserung der lebensfähigkeit kryokonservierter zellen
EP2362733A4 (de) Materialien und verfahren, zum verbessern der hämatopoetischen stammzelle-engraftment prozedur
EP2209888A4 (de) Verbesserte verfahren zur herstellung von rpe-zellen und zusammensetzungen von rpe-zellen
DK2574677T3 (da) Fremgangsmåder til celledyrkning
EP2344622A4 (de) Vorrichtung zur kultivierung verankerungsabhängiger zellen
EP2376920A4 (de) Verfahren zur erkennung von änderungen in zellen
IL196322A0 (en) Improved process for the culturing of cells
GB2490443B (en) Organ regeneration method utilizing iPS cell and blastocyst complementation
IL214529A0 (en) Bioreactor for the cultivation of mammalian cells and methods of use thereof
EP2305792A4 (de) Kryokonservierungszusammensetzung für zelle und gewebe
EP2637702A4 (de) Verfahren, zusammensetzungen, zellen und kits zur behandlung von ischämischen verletzungen
EP2795293A4 (de) Spektroskopische vorrichtung und verfahren zur identifizierung von mikroorganismen in kulturen
EP2405959A4 (de) Verfahren, vorrichtungen und kits zur einführung von genetischem material in lebende zellen
PT2459702T (pt) Meio de cultura de células para a expressão da proteína adamts
EP2169049A4 (de) Zellkulturbehälter und zellkuklturverfahren
EP2462622A4 (de) Verfahren und vorrichtung zur metallisierung von solarzellen
EP2683812A4 (de) Verfahren zur zelltransfektion mit nukleinsäuren

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20111013

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR

DAX Request for extension of the european patent (deleted)
A4 Supplementary search report drawn up and despatched

Effective date: 20130917

RIC1 Information provided on ipc code assigned before grant

Ipc: C12M 1/42 20060101ALI20130911BHEP

Ipc: C12M 3/00 20060101ALI20130911BHEP

Ipc: A61M 5/00 20060101AFI20130911BHEP

Ipc: C12N 15/89 20060101ALI20130911BHEP

17Q First examination report despatched

Effective date: 20150824

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: EXAMINATION IS IN PROGRESS

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20241001