EP2444546A1 - Procédé de biomarquage de matériaux textiles - Google Patents

Procédé de biomarquage de matériaux textiles Download PDF

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Publication number
EP2444546A1
EP2444546A1 EP10188180A EP10188180A EP2444546A1 EP 2444546 A1 EP2444546 A1 EP 2444546A1 EP 10188180 A EP10188180 A EP 10188180A EP 10188180 A EP10188180 A EP 10188180A EP 2444546 A1 EP2444546 A1 EP 2444546A1
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EP
European Patent Office
Prior art keywords
biomarker
textile
biomarked
yarn
aqueous solution
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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EP10188180A
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German (de)
English (en)
Inventor
Ilaria Donelli
Giuseppe Rosace
Giorgio Rondi
Francesca Isella
Gabriella Alberti Fusi
Sergio Calleri
Giuliano Freddi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centro Tessile Cotoniero e Abbigliamento SpA
Felli Color SpA
Linificio e Canapificio Nazionale SpA
Stazione Sperimentale per la Seta
Original Assignee
Centro Tessile Cotoniero e Abbigliamento SpA
Felli Color SpA
Linificio e Canapificio Nazionale SpA
Stazione Sperimentale per la Seta
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Application filed by Centro Tessile Cotoniero e Abbigliamento SpA, Felli Color SpA, Linificio e Canapificio Nazionale SpA, Stazione Sperimentale per la Seta filed Critical Centro Tessile Cotoniero e Abbigliamento SpA
Priority to EP10188180A priority Critical patent/EP2444546A1/fr
Publication of EP2444546A1 publication Critical patent/EP2444546A1/fr
Withdrawn legal-status Critical Current

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    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
    • D06M16/00—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic
    • D06M16/003—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic with enzymes or microorganisms
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06H—MARKING, INSPECTING, SEAMING OR SEVERING TEXTILE MATERIALS
    • D06H1/00—Marking textile materials; Marking in combination with metering or inspecting
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
    • D06M15/00—Treating fibres, threads, yarns, fabrics, or fibrous goods made from such materials, with macromolecular compounds; Such treatment combined with mechanical treatment
    • D06M15/01—Treating fibres, threads, yarns, fabrics, or fibrous goods made from such materials, with macromolecular compounds; Such treatment combined with mechanical treatment with natural macromolecular compounds or derivatives thereof
    • D06M15/15—Proteins or derivatives thereof
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
    • D06M23/00—Treatment of fibres, threads, yarns, fabrics or fibrous goods made from such materials, characterised by the process
    • D06M23/06—Processes in which the treating agent is dispersed in a gas, e.g. aerosols

Definitions

  • the present invention relates to the field of textile materials and provide a method for biomarking such materials in order to allow identification, authentication or tracking of the materials and their manufacturers.
  • the problem of identification, authentication or tracking of textile materials exists not only for finished articles and clothes, but also for intermediates and semi-finished products.
  • the structure of the textile sector is formed by SMEs (>95%), the majority of which are commission companies specialised in one or few processes, receiving textile materials (yarns and/or fabrics and/or garments), applying the requested treatment and then sending the treated textiles back to their clients or, more often, to the next component of the textile and clothing supply chain, i.e. a weaver, dyer, printer, finisher, or to an end-user, i.e. garment maker, distributor, retailer, etc.
  • markers directly integrated into the textile substrates are either fibres, yarns, fabrics or garments, either applied on a predetermined area or homogeneously distributed in all their parts, is probably a more attractive and effective approach to solve the above mentioned problems.
  • the markers used for textile substrates must not degrade their properties, must be easy to apply, not detectable under normal visual or hand inspection but unequivocally detectable by means of appropriate portable or laboratory tools to allow goods being identified with certainty.
  • Fluorescent or phosphorescent organic and inorganic dyes and pigments, ultraviolet emitting, near/mid infrared detectable compounds, magnetic or reflective nano-particles comprising metal tracers are some of the anti-counterfeit markers proposed for use onto textile substrates. These markers can be added to polymer melt or dope before spinning the fibres. Thus, intrinsically marked synthetic fibres can be produced and then transformed into yarns and fabrics to manufacture 100% marked textile goods, or blended in different proportions with other natural or synthetic fibres to manufacture textile goods containing variable proportions of marked fibres.
  • already made natural and synthetic fibres can be treated at different processing stages (from sorting, carding, spinning to dyeing, printing and finishing) with suitable processing aids containing the marker in order to obtain an homogeneous distribution of the same onto the textile substrate and, consequently, onto the textile good produced therefrom.
  • processing aids containing the marker in order to obtain an homogeneous distribution of the same onto the textile substrate and, consequently, onto the textile good produced therefrom.
  • Interference with conventional textile auxiliaries, dyes, and finishing products must be avoided as it may impair aesthetic and functional properties of textile goods.
  • Bio-based approaches have been proposed to produce counterfeit-proof textile products, to investigate frauds in the textile market (from fibres to garments), and to determine the authenticity as well as the origin of the textile materials.
  • immunological techniques can be used to qualitatively and quantitatively identify the presence of different animal fibres in an article. The method is based upon the production and use of monoclonal antibodies to recognize the species specific sequences of the primary structure of keratin for identifying fibres from the different animal species ( EP 1 847 831 A1 ).
  • DNA technology is currently being pursued to mark textiles for authenticating purposes.
  • Appropriately selected DNA markers can be directly incorporated into various kinds of processing media and then applied onto textile substrates by means of conventional textile technologies to obtain marked textile products to be manufactured into different goods ( WO 2004/094713 A2 ; WO 2007/035581 A2 ).
  • a method for authenticating textile articles by labelling with an optical reporter marker linked to a nucleic acid tag, detecting the optical reporter and then characterizing or verifying the nucleic acid sequencing, genotyping or like techniques has also been reported ( US 20080299559 ).
  • the marker could be either solid or liquid and applied to a predetermined area of the garment. Textiles may have a label with the manufacturer name on it and may also be used as a region of the garment which the optical reporter marker is placed.
  • the present invention describes a process designed to integrate biomarkers into textile substrates.
  • the stage of application and related application conditions are selected so as to ensure stability and durability of the biomarker activity for the required shelf life and life time span.
  • the biomarkers of the invention are proteins, preferably endowed with biocatalytic activity. Their presence is detected by means of specific activity tests resulting, more often but non exclusively, in colour changes of a solution containing a compound acting as substrate of the biocatalyst. Visual inspection of the reaction is generally sufficient as identification or authentication tool, but specific instruments, i.e. a UV/Vis spectrophotometer, can also be used to detect the presence of the biomarker.
  • the biomarker carrier media can include aqueous solutions of polymers, adhesives, binders, cross-linking agents, textile auxiliaries, softeners, lubricants, textile finishes (antistatic, water-proof, softening, antimicrobial agents or the like).
  • Application of the biomarker can be made preferably but not exclusively during finishing, more generally at any processing stage where a treatment under wet conditions can be carried out.
  • the invention is directed to any kind of textile materials in form of loose fibres, tops, tow, sliver, yarn, fabric (woven, knitted, nonwoven, braided, etc.), garment.
  • Textile materials can be made of natural or man-made (artificial, synthetic) fibres or their blends. End uses of biomarked textile products may comprise apparel, household, decoration, furnishing, industrial and technical textiles.
  • the present invention relates to a method for biomarking a textile material, comprising the step of putting the said textile material into contact with an aqueous solution containing a biomarker substance for a time and in conditions sufficient to allow the said biomarker substance to be retained by the said textile material in a detectable amount, wherein the said biomarker substance is a protein that is preferably able to interact with a substrate that can undergo a detectable reaction upon contact with such a protein.
  • the method of the invention is used to mark textile materials in any form and at any processing stage where a treatment under wet conditions can be carried out.
  • Non limiting examples will be given herein below, which include textile materials from sliver to yarn, fabric and garment.
  • the biomarker will be made to intimately adhere to the individual fibres constituting the textile material by means of current textile technologies.
  • the intermediate and semi-finished biomarked textile materials of the invention thus obtained can be used to manufacture fully biomarked textile items or can be blended or otherwise included into other textile and non textile products thus endowing them with an effective biomarking function.
  • End uses of biomarked textile materials can be apparel, household, decoration, furnishing, industrial and technical textiles.
  • the biomarker of the invention is a protein preferably endowed with biocatalytic activity.
  • Protein biocatalysts typically belong to the following classes: oxidoreductases, transferases, hydrolases, lyases, isomerases, ligases.
  • Biocatalysts are commercially available products supplied in form of granules, powder or aqueous solution. Before use, the biocatalyst activity is tested as received by means of specific testing methods provided by the supplier or available from the scientific and technical literature. This allows determining the activity per unit weight or volume necessary to define the biomarkers concentration in the application medium.
  • the biomarker is selected as a function of the kind of textile material to be treated (the biocatalyst must be inert against the polymeric textile material), of the processing step identified for its application (application conditions - i.e. pH, solvent, time, temperature, etc. - must not impair the biocatalyst activity), and taking into account the compatibility with other components of the application medium, i.e. textile auxiliaries and finishing products.
  • Typical non compatible biocatalyst/textile combinations are: proteases on protein fibres (eg. wool and silk); cellulases on cellulosic fibres (cotton, linen, hemp, viscose, lyocell, etc.); lipases and esterases on polyester fibres; proteases and amidases on polyamide fibres; nitrilases on acrylic fibres. Under suitable ambient conditions (i.e. temperature, humidity) all these biocatalysts are able to hydrolyze the respective polymeric material constituting the fibres, thus leading to fibre degradation.
  • oxidoreductases such as laccases and other polyphenol oxidases able to use air oxygen as co-substrate, must be avoided because their activation and reaction with the textile substrate may lead to colour change (more or less extensive browning of fibres).
  • Biocatalysts suffer from exposition to high temperatures and/or extreme acidic or alkaline pHs for long times. To avoid any risk of loosing totally or partially the biocatalyst activity during application to textiles, the processing conditions under which the biomarker can be safely applied to the textile material are as follows:
  • the compatibility of the biocatalyst with the other components of the application medium which may include textile auxiliaries (surfactants, wetting and levelling agents, salts, dyes, emulsifiers, lubricants, antifoam agents, etc.) and/or finishing products (softeners, antistatic, water-proof, soil release, antimicrobial, fire-proofing, agents; binders, polymers, cross-linking agents, etc.) is tested before application by mixing the required amount of biocatalyst with the other recipe components and then performing the activity testing procedure provided by the supplier or available from the scientific and technical literature. This allows determining the activity of the biomarker per unit weight or volume of application medium and, consequently, the amount of biomarker that will be transferred onto the textile material by pad-drying or exhaust processing conditions.
  • textile auxiliaries surfactants, wetting and levelling agents, salts, dyes, emulsifiers, lubricants, antifoam agents, etc.
  • finishing products softeners, antistatic
  • An important advantage of the method of the invention is that the wide range of commercial protein biocatalysts available allows setting up a large number of unique biomarker/textile material combinations endowed with high specificity, thus making counterfeit of the biomarking method and of the biomarked textile material very difficult from one side and identification or verification of the same more straightforward from the other side.
  • Embodiments of the invention have the advantage that textile materials can be conveniently and unequivocally marked at a selected processing step with a solution containing the biomarker prepared on purpose. The costs related to the biomarking process are very low because the biomarker is applied in catalytic amounts.
  • Another advantage is that biomarking can be repeated several times along the manufacturing chain to ensure the required durability. The intrinsic aesthetic and functional properties and performance of the textile material are not affected by the presence of the biomarker which is not visible at the naked eye. Authentication techniques apply the concept of biosensing.
  • Textile fibres that can be treated with the method of the invention include all natural (both plant and animal fibres, including but not limited to cotton, flax, hemp, wool, silk), man-made fibres (including but non limited to polyester, polyamide, acrylic, polyolefin, regenerated cellulose fibres) and their blends in any range of composition. Staple and continuous filament fibres are all included. Accordingly, two and three dimensional textile constructions obtained by carding, combing, spinning, weaving, knitting, nonwoven bonding, braiding, knotting, laying, and plating are possible materials for the inventive method.
  • Methods of application comprise all, but not exclusively, the currently available technologies which bring fluids in close contact with the individual fibres forming the textile material thus favouring adsorption, adhesion and/or physical and/or chemical bonding of the active ingredients contained in the fluid onto the fibre surface or diffusion, penetration and anchoring in the fibre bulk.
  • These technologies include but are not limited to batch processes, where both the fluid and the textile material move with respect to one another or only one of them (the fluid or the textile material) is moving and the other is stationary, as well as continuous processes generally comprising the impregnation of the textile material with a high concentrated solution of the processing aids made by padding, coating or spraying, followed by drying and fixation/curing steps.
  • Natural and man-made staple fibres and their blends undergo a series of operations, such as opening, carding, combing, roving before being fed to the spinning machine to make the yarn. All these processes are performed under dry conditions, but wetting the fibrous materials with an aqueous solution containing auxiliaries like lubricating, softening, and antistatic agents may be required to lower the negative effects of fibre-fibre and fibre-machine friction and to reduce electrostatic charge building under the strong mechanical stresses caused by the process.
  • spraying heads mounted on a drawing frame of a combing machine are fed with an aqueous solution containing the lubricant/antistatic agent and the biomarker.
  • an aqueous solution containing the lubricant/antistatic agent and the biomarker.
  • a peroxidase biomarker EC 1.11.1.7
  • the solution is buffered at pH 6 with 0.1 M phosphate buffer.
  • the running sliver is sprayed at room temperature in such a way that the wet pick up is 10-30 w%, preferably 20 w%, and the amount of active ingredients taken up by fibres is 1-5 w% for the lubricant and 0.1-1 w% for the biomarker.
  • the spraying treatment results in a biomarked sliver which will end up with a biomarked yarn when fed to a spinning machine.
  • Flax processing before spinning has specific features. Flax slivers undergo a series of doubling and drafting operations resulting in the production of a flax rove. The latter is scoured to eliminate non cellulosic substances and to help primary fibres to slide during spinning. Bleaching under oxidizing conditions at alkaline pH may also be performed.
  • the flax rove is neutralized and the neutralizing solution discharged. Then, an aqueous solution containing a wetting agent and the biomarker is circulated from inside to outside of the perforated support onto which the rove is rolled.
  • a solution containing 1-5 g/L, preferably 2-3 g/L, of a non-ionic alkyl alcohol ethoxylate surfactant and 1-10 g/L, preferably 4-6 g/L, of protease biomarker (EC 3.4.21.62) is heated at 30-60°C, preferably 40-50°C, and circulated in the machine containing the rolls of flax rove for 10-30 min, preferably 15-20 min.
  • the material-to-liquor ratio is typically from 1:10 to 1:30.
  • the optimum absorption level for the biomarker is in the range 0.1-1 w%.
  • Wet spinning is a specific flax spinning process leading to yarns of remarkable quality and fineness.
  • the spinning machine is equipped with a trough full of water located on top of each spinning head upstream to the drafting frame.
  • the flax rove passes through it before entering the drafting frame.
  • the water is substituted with an aqueous solution containing a wetting agent and the biomarker
  • the rove passes through the aqueous solution contained in the basin thus picking up the solution containing the biomarker.
  • the rove enters the drafting frame where the yarn is formed and collected on spools.
  • the biomarked yarn thus produced comprises flax fibres with a biomarker strongly adhering to their surface and physically entrapped into the fibrous network.
  • the impregnation trough is filled up with an aqueous solution containing catalase (EC 1.11.1.6) as biomarker (1-20 g/L, preferably 5-10 g/L), buffered at pH 7 with 50 mM phosphate buffer.
  • the solution is maintained at room temperature.
  • the level of the solution in the trough is controlled in order to ensure that the rove is always well immersed in the solution.
  • Typical wet pick up values are 30-100 w%, preferably 50-80 w%.
  • the optimum absorption level for the biomarker is in the range 0.1-1 w%.
  • the biomarked rove is transformed into a biomarked yarn, which is collected on 100 g spools.
  • the biomarked flax yarns coming from several spools are transferred onto packages of about 1 kg ready to be delivered to the knitter and/or weaver.
  • a polyacrylic resin at 0.2-2 g/L, preferably 0.5-1 g/L, is added to the biomarker solution, together with a polyfunctional aziridine cross-linker at 0.5-4 w% of the resin weight, preferably 1.5-2.5 w%.
  • the binding strength of the biomarker with fibres and its stability are enhanced under these application conditions.
  • Biomarking can be executed on the yarn already rolled onto cones just before delivery to the weaver.
  • a machine used to wrap up cones of flax yarn is equipped with humidifying units which are fed with an aqueous solution containing an antimould agent and the biomarker.
  • the solution is forced to pass through the perforated cone from inside to outside at predetermined pressure and time.
  • the process results in biomarked yarn which comprises fibres impregnated with the biomarker.
  • the biomarker is a catalase (EC 1.11.1.6) at 1-10 g/L, preferably 3-6 g/L, buffered at pH 7 with 50 mM phosphate buffer. It is mixed with benzo-isothiazolinone as antimould agent at 0.1-2 g/L, preferably 0.5-1 g/L. Then the solution is applied to the cone in such a way that the wet pick up is 1-20 w%, preferably 5-10 w%, and the amount of biomarker taken up by the yarn is 0.1-5 w%. This treatment results in a biomarked yarn.
  • Yarns made of natural or man-made fibres or their blends usually undergo a processing cycle comprising scouring, dyeing and finishing. Treatments are carried out on yarns in hank or cone form.
  • hanks of up to 1 kg each are processed into cabinets of up to 800 kg capacity, working with a material-to-liquor ratio up to 1:15.
  • Cones up to about 1.2 kg each are processed into package dyeing machines of up to 600 kg capacity, working with a material-to-liquor ratio up to 1:10.
  • the yarn material is stationary while the fluid is flowing through the hank or cone at a controlled speed and for the number of cycles required by the treatment.
  • Time-temperature processing diagrams are computer controlled and can be tailored to the properties of the material under processing.
  • Yarn finishing typically consists in a wet treatment able to impart softness and lubricity to the material thus improving handle and reducing friction during subsequent weaving, knitting, etc.
  • Most softeners are cationic surfactants which bind by electrostatic attraction to the negatively charged groups at the fibre surface. They are based on quaternary ammonium salts with one or two long alkyl chains. Others can be derived from imidazolium, substituted amine salts, or quaternary alkoxy ammonium salts. Silicone based compounds such as polydimethylsiloxane modified to contain amine or amide groups bind to the fibres and work by lubricating agents, thus imparting improved feel.
  • a finishing bath is circulated in the cabinet or package dyeing machine.
  • the processing cycle is started. The temperature is raised from ambient to about 40°C and kept stationary for 20 min while the solution is circulated in the machine.
  • the biomarked yarn thus produced comprises fibres with the biomarker intimately adhering to their surface.
  • Yarns are usually transferred from bobbins to other supports like cones of different sizes before the next processing stages like weaving or knitting.
  • winding machines are used.
  • the yarn coming from the unwinding unit is guided to pass onto the surface of a roller partially immersed in an aqueous solution of the biomarker.
  • the yarn picks up the solution while passing onto the roller surface thus becoming impregnated with the biomarker.
  • the amount of solution picked up depends on the kind of yarn, on its construction, on the speed of the roller, etc. Afterwards the biomarked yarn reaches the take up unit and is wrapped on the destination cone.
  • the impregnating solution contains a non-ionic alkyl alcohol ethoxylate surfactant at 2-10 g/L, preferably 4-6 g/L, and peroxidase (EC 1.11.1.7) as the biomarker.
  • concentration of the biomarker is about 2-40 g/L, preferably 10-20 g/L.
  • the yarns passing onto the roller picks up an amount of solution of about 30-80 w%, preferably 40-60 w% before reaching the winding head.
  • the optimum absorption level for the biomarker is in the range 0.1-2 w%.
  • Woven or knitted fabrics are usually finished to impart on them the visual, physical, and aesthetic properties demanded by end-users and consumers.
  • a softening treatment represents a general approach for finishing many types of textile articles. However, more specific finishing treatments can be performed such as: antimicrobial, anti-pilling, crease resist, wash and wear, antistatic, hydrophilic or hydrophobic finish, etc., depending on the kind of textile and end-use destination.
  • the finishing treatment usually takes place as a separate operation after dyeing/printing. Finishes can be applied on fabrics by continuous (typically: padding techniques) or discontinuous processes (typically: exhaust techniques).
  • a wool knitted fabric is subjected to a softening treatment in an overflow machine.
  • the material-to-liquor ratio is from 1:10 to 1:30.
  • the temperature is raised from ambient to about 40°C and kept stationary for 20 min while the solution is circulated in the machine. Then the bath is discharged, the fabric is removed and dried.
  • the optimum absorption level for the biomarker is in the range 0.1-1 w%.
  • the biomarked fabric thus produced comprises fibres with the biomarker intimately adhering to their surface.
  • a flax woven fabric is subjected to a softening treatment in a jigger machine, which operates on open width by transferring the fabric back and forth from roller to roller via the medium of a softening solution located at the base of the machine.
  • the softening bath solution contains a cationic softener at a concentration of 1-5 g/L, preferably 2-3 g/L, at pH 5 (adjusted with acetic acid), to which a protease biomarker (EC 3.4.21.62) is added at a concentration of 1-5 g/L, preferably 2-3 g/L.
  • the material-to-liquor ratio is from 1:2 to 1:4.
  • the number of passages can be two or more.
  • the temperature is raised from ambient to about 40°C and kept stationary until completion of the selected number of passages. Then the bath is discharged, the fabric is removed and dried.
  • the optimum absorption level for the biomarker is in the range 0.1-1 w%.
  • the biomarked fabric thus produced comprises fibres with the biomarker intimately adhering to their surface.
  • a cotton woven fabric is impregnated in a foulard with a solution containing a cationic softener at a concentration of 1-10 g/L, preferably 3-6 g/L, at pH 5 (adjusted with acetic acid), to which a protease biomarker (EC 3.4.21.62) is added at a concentration of 1-20 g/L, preferably 5-10 g/L.
  • Typical wet pick up values are 30-100 w%, preferably 50-80 w%.
  • the fabric passes through the drying unit operated under the conditions definedabove.
  • the optimum absorption level for the biomarker is in the range 0.1-1 w%.
  • the biomarked fabric thus produced comprises fibres with the biomarker intimately adhering to their surface.
  • the following protocol refers to the detection of the bioactivity of any form of textile material biomarked with catalase biocatalyst (EC 1.11.1.6).
  • a flax yarn biomarked with catalase is sampled in form of a small hank of about 1 g.
  • An aqueous solution of hydrogen peroxide (H 2 O 2 ) in 50 mM phosphate buffer pH 7 is prepared by diluting the 30 w% stock solution from 30 to 200 times, preferably from 80 to 120 times, in order to have a H 2 O 2 concentration in the range of 0.2-1 w%.
  • the test is carried out at room temperature.
  • the biomarked flax hank is immersed into the H 2 O 2 solution.
  • the catalase reaction follows the scheme: 2 H 2 O 2 ⁇ 2 H 2 O + O 2
  • the oxygen gas formed by the reaction is released in form of bubbles as soon as the yarn is immersed in the H 2 O 2 solution.
  • a non-biomarked yarn is immersed into the H 2 O 2 solution. No bubbles formation is observed under these circumstances.
  • the protocol of this example refers to the detection of the bioactivity of any form of textile material biomarked with peroxidase biocatalyst (EC 1.11.1.7).
  • a small hank of about 1 g of cotton yarn biomarked with peroxidase is prepared.
  • a 1 w% solution of pyrogallol (1,2,3-trihydroxybenzene) in 0.1 M phosphate buffer pH 6 is prepared (Reagent A).
  • a fixed volume of 30 w% H 2 O 2 solution (Reagent B) is diluted 100 times with water. The test is carried out at room temperature.
  • the 1 g hank of biomarked yarn is immersed in 100 ml of Reagent A.
  • the protocol of this example refers to the detection of the bioactivity of any form of textile material biomarked with protease biocatalyst (EC 3.4.21.62).
  • a small piece of cotton fabric biomarked with protease having a size of 1 to 4 cm 2 , corresponding from about 10 to 100 mg of material, is cut and used for the test.
  • a 100 mM HEPES NaOH buffer pH 7.5 is prepared (Reagent A).
  • a 20 mM solution of N-Succinyl-Ala-Ala-Pro-Phe p-Nitroanilide solution is prepared in Dimethyl sulfoxide (Reagent B). The test is carried out at 37°C.

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  • Engineering & Computer Science (AREA)
  • Textile Engineering (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Materials Engineering (AREA)
  • Dispersion Chemistry (AREA)
  • Chemical Or Physical Treatment Of Fibers (AREA)
EP10188180A 2010-10-20 2010-10-20 Procédé de biomarquage de matériaux textiles Withdrawn EP2444546A1 (fr)

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WO2016100503A1 (fr) * 2014-12-16 2016-06-23 Apdn (B.V.I.) Inc. Procédé et dispositif de marquage de matériaux fibreux
WO2017180302A1 (fr) * 2016-04-11 2017-10-19 Apdn (B.V.I.) Inc. Procédé de marquage de produits cellulosiques
US9904734B2 (en) 2013-10-07 2018-02-27 Apdn (B.V.I.) Inc. Multimode image and spectral reader
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US10920274B2 (en) 2017-02-21 2021-02-16 Apdn (B.V.I.) Inc. Nucleic acid coated submicron particles for authentication
US10995371B2 (en) 2016-10-13 2021-05-04 Apdn (B.V.I.) Inc. Composition and method of DNA marking elastomeric material
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CN113699773A (zh) * 2021-08-24 2021-11-26 广东溢达纺织有限公司 筒纱是否存在内外色差的判断方法

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