EP2596096A4 - Verfahren zur induktion der differenzierung menschlicher pluripotenter stammzellen in intermediäre mesodermzellen - Google Patents

Verfahren zur induktion der differenzierung menschlicher pluripotenter stammzellen in intermediäre mesodermzellen

Info

Publication number
EP2596096A4
EP2596096A4 EP11809770.8A EP11809770A EP2596096A4 EP 2596096 A4 EP2596096 A4 EP 2596096A4 EP 11809770 A EP11809770 A EP 11809770A EP 2596096 A4 EP2596096 A4 EP 2596096A4
Authority
EP
European Patent Office
Prior art keywords
human
cell
pluripotent stem
inducing differentiation
stem cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP11809770.8A
Other languages
English (en)
French (fr)
Other versions
EP2596096B1 (de
EP2596096A1 (de
Inventor
Kenji Osafune
Shin-Ichi Mae
Masatoshi Kajiwara
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kyoto University NUC
Original Assignee
Kyoto University NUC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kyoto University NUC filed Critical Kyoto University NUC
Publication of EP2596096A1 publication Critical patent/EP2596096A1/de
Publication of EP2596096A4 publication Critical patent/EP2596096A4/de
Application granted granted Critical
Publication of EP2596096B1 publication Critical patent/EP2596096B1/de
Not-in-force legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5073—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Transplantation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP11809770.8A 2010-07-21 2011-07-21 Verfahren zur induktion der differenzierung menschlicher pluripotenter stammzellen in intermediäre mesodermzellen Not-in-force EP2596096B1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US36637910P 2010-07-21 2010-07-21
US201161451825P 2011-03-11 2011-03-11
PCT/JP2011/067181 WO2012011610A1 (en) 2010-07-21 2011-07-21 Method for inducing differentiation of human pluripotent stem cell into intermediate mesoderm cell

Publications (3)

Publication Number Publication Date
EP2596096A1 EP2596096A1 (de) 2013-05-29
EP2596096A4 true EP2596096A4 (de) 2014-01-08
EP2596096B1 EP2596096B1 (de) 2018-01-31

Family

ID=45497017

Family Applications (1)

Application Number Title Priority Date Filing Date
EP11809770.8A Not-in-force EP2596096B1 (de) 2010-07-21 2011-07-21 Verfahren zur induktion der differenzierung menschlicher pluripotenter stammzellen in intermediäre mesodermzellen

Country Status (4)

Country Link
US (1) US9121011B2 (de)
EP (1) EP2596096B1 (de)
JP (1) JP5896360B2 (de)
WO (1) WO2012011610A1 (de)

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US9890357B2 (en) 2011-12-19 2018-02-13 Kyoto University Method for inducing differentiation of human pluripotent stem cells into intermediate mesoderm cells
JP6112733B2 (ja) * 2012-04-06 2017-04-12 国立大学法人京都大学 エリスロポエチン産生細胞の誘導方法
KR20130119683A (ko) * 2012-04-24 2013-11-01 사회복지법인 삼성생명공익재단 줄기세포 배양 배지 및 이를 이용한 줄기세포의 배양방법
EP2976044A4 (de) * 2013-03-21 2016-11-23 Univ Columbia Zusammensetzungen und verfahren zur zahngeweberegeneration
JP6461787B2 (ja) * 2013-04-12 2019-01-30 国立大学法人京都大学 肺胞上皮前駆細胞の誘導方法
KR102145967B1 (ko) 2013-06-11 2020-08-19 고쿠리츠 다이가쿠 호진 교토 다이가쿠 신장 전구세포의 제조 방법 및 신장 전구세포를 포함하는 의약
RU2016100232A (ru) 2013-06-14 2017-07-17 Дзе Юниверсити Оф Квинсленд Почечные клетки-предшественники
MX2015017330A (es) * 2013-07-29 2016-04-06 Hoffmann La Roche Metodo para la diferenciacion de celulas madre pluripotenciales en precursores renales multi-competentes.
JP6455729B2 (ja) * 2013-10-18 2019-01-23 国立大学法人 熊本大学 多能性幹細胞からの腎臓誘導法
WO2016085765A1 (en) 2014-11-25 2016-06-02 President And Fellows Of Harvard College Methods for generation of podocytes from pluripotent stem cells and cells produced by the same
AU2014277667B2 (en) 2014-12-15 2022-07-14 The University Of Queensland Differentiation of pluripotent stem cells to form renal organoids
CA2978870C (en) 2015-03-06 2024-06-25 Kyoto University Method for inducing differentiation of alveolar epithelial cells
US10563171B2 (en) 2015-06-29 2020-02-18 Kyoto University Method for inducing differentiation of pluripotent stem cells into germ cells
JP6646311B2 (ja) * 2015-07-17 2020-02-14 国立大学法人京都大学 多能性幹細胞から中胚葉前駆細胞および血液血管前駆細胞への分化誘導法
DK3347456T3 (da) * 2015-09-08 2024-02-19 Us Health Fremgangsmåde til reproducerbar differentiering af retinale pigmentepitelceller af klinisk kvalitet
ES2897974T3 (es) 2015-09-11 2022-03-03 Astellas Pharma Inc Método para producir células progenitoras renales
CN105850980B (zh) * 2016-04-14 2018-06-12 广州赛莱拉干细胞科技股份有限公司 一种角膜缘干细胞的冻存液及冻存方法
US11268950B2 (en) 2016-09-28 2022-03-08 Organovo, Inc. Use of engineered renal tissues in assays
EP3633026A4 (de) 2017-05-25 2021-03-10 Kyoto University Verfahren zur induzierung der differenzierung einer mesodermalen zwischenzelle zu einer nierenvorläuferzelle und verfahren zur induktion der differenzierung einer pluripotenten stammzelle zu einer nierenvorläuferzelle
US20210123027A1 (en) 2018-10-10 2021-04-29 National University Corporation Tottori University Method for producing human induced pluripotent stem cells containing exogenous chromosome
JP7437766B2 (ja) * 2018-10-31 2024-02-26 国立大学法人京都大学 中内胚葉系への分化抵抗性が解除された多能性幹細胞の作製方法
EP3950933A4 (de) 2019-03-29 2023-01-11 Kaneka Corporation Zellpopulation mit pluripotenten stammzellen und verfahren zu ihrer herstellung
CN110656089B (zh) * 2019-10-15 2021-02-09 上海捷易生物科技有限公司 一种将iPS细胞定向诱导分化到成熟神经元的方法
US11459372B2 (en) 2020-11-30 2022-10-04 Crispr Therapeutics Ag Gene-edited natural killer cells
WO2022144632A1 (en) 2020-12-30 2022-07-07 Crispr Therapeutics Ag Compositions and methods for differentiating stem cells into nk cells
CN118984871A (zh) * 2022-02-15 2024-11-19 国立大学法人神户大学 制备人多能干细胞衍生的睾丸间质样细胞和人多能干细胞衍生的睾丸间质样细胞群的方法

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RYUJI MORIZANE ET AL: "Differentiation of murine embryonic stem and induced pluripotent stem cells to renal lineage in vitro", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US, vol. 390, no. 4, 25 December 2009 (2009-12-25), pages 1334 - 1339, XP008152385, ISSN: 0006-291X, [retrieved on 20091031], DOI: 10.1016/J.BBRC.2009.10.148 *
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Also Published As

Publication number Publication date
JP2013530680A (ja) 2013-08-01
EP2596096B1 (de) 2018-01-31
US9121011B2 (en) 2015-09-01
US20130122536A1 (en) 2013-05-16
WO2012011610A1 (en) 2012-01-26
JP5896360B2 (ja) 2016-04-13
EP2596096A1 (de) 2013-05-29

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