EP2689006A4 - Expression in situ de lipase pour production enzymatique d'alcool esters pendant la fermentation - Google Patents

Expression in situ de lipase pour production enzymatique d'alcool esters pendant la fermentation

Info

Publication number
EP2689006A4
EP2689006A4 EP12760739.8A EP12760739A EP2689006A4 EP 2689006 A4 EP2689006 A4 EP 2689006A4 EP 12760739 A EP12760739 A EP 12760739A EP 2689006 A4 EP2689006 A4 EP 2689006A4
Authority
EP
European Patent Office
Prior art keywords
lipase
during fermentation
alcohol esters
enzymatic production
situ expression
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP12760739.8A
Other languages
German (de)
English (en)
Other versions
EP2689006A2 (fr
Inventor
Robert Dicosimo
Arthur Leo Kruckeberg
Aken Thomas Edwin Van
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Butamax Advanced Biofuels LLC
Original Assignee
Butamax Advanced Biofuels LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Butamax Advanced Biofuels LLC filed Critical Butamax Advanced Biofuels LLC
Publication of EP2689006A2 publication Critical patent/EP2689006A2/fr
Publication of EP2689006A4 publication Critical patent/EP2689006A4/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/649—Biodiesel, i.e. fatty acid alkyl esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16—Butanols
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Fodder In General (AREA)
EP12760739.8A 2011-03-23 2012-03-23 Expression in situ de lipase pour production enzymatique d'alcool esters pendant la fermentation Withdrawn EP2689006A4 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161466712P 2011-03-23 2011-03-23
US201161498292P 2011-06-17 2011-06-17
PCT/US2012/030468 WO2012129548A2 (fr) 2011-03-23 2012-03-23 Expression in situ de lipase pour production enzymatique d'alcool esters pendant la fermentation

Publications (2)

Publication Number Publication Date
EP2689006A2 EP2689006A2 (fr) 2014-01-29
EP2689006A4 true EP2689006A4 (fr) 2014-10-01

Family

ID=46880081

Family Applications (1)

Application Number Title Priority Date Filing Date
EP12760739.8A Withdrawn EP2689006A4 (fr) 2011-03-23 2012-03-23 Expression in situ de lipase pour production enzymatique d'alcool esters pendant la fermentation

Country Status (8)

Country Link
EP (1) EP2689006A4 (fr)
JP (1) JP2014509869A (fr)
CN (1) CN103459587A (fr)
AU (1) AU2012230730A1 (fr)
BR (1) BR112013024329A2 (fr)
CA (1) CA2829941A1 (fr)
WO (1) WO2012129548A2 (fr)
ZA (1) ZA201306647B (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106399286A (zh) * 2016-10-18 2017-02-15 江南大学 一种促进毕赤酵母产碱性果胶酶的方法

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013016717A2 (fr) * 2011-07-28 2013-01-31 Butamax(Tm) Advanced Biofuels Llc Enzymes céto-isovalérate décarboxylase et leurs procédés d'utilisation
WO2015009601A1 (fr) * 2013-07-16 2015-01-22 Butamax Advanced Biofuels Llc Levure ayant une meilleure tolérance au butanol comprenant des protéines pariétales
CN107488646B (zh) * 2016-06-10 2019-12-10 广东溢多利生物科技股份有限公司 脂肪酶突变体TTL-Arg59Ser/Gly60Glu/Ser61Asn/Ile62Val及其基因和应用
CN107488644B (zh) * 2016-06-10 2019-12-10 广东溢多利生物科技股份有限公司 一种热稳定性提高的脂肪酶TTL突变体TTL-Gly60Glu/Ser61Asn及其基因和应用
CN107488648B (zh) * 2016-06-10 2019-12-10 广东溢多利生物科技股份有限公司 热稳定性提高的脂肪酶TTL突变体TTL-Arg59Ser/Gly60Glu/Ser61Asn及基因和应用
CN107488647B (zh) * 2016-06-10 2019-12-10 广东溢多利生物科技股份有限公司 一种热稳定性提高的脂肪酶TTL突变体TTL-Gly60Glu及其基因和应用
CN107488645B (zh) * 2016-06-10 2019-12-10 广东溢多利生物科技股份有限公司 一种热稳定性提高的脂肪酶TTL突变体TTL-Ser61Asn及其基因和应用
CN106701606B (zh) * 2016-12-19 2021-03-26 广东利世康低碳科技有限公司 一种能降解利用餐厨废弃物的基因工程产朊假丝酵母及其构建方法
EP3630880B1 (fr) 2017-05-24 2025-12-17 POET Research, Inc. Compositions d'huile contenant de l'ester d'alkyle amélioré, et procédés de production et d'utilisation desdites compositions
US12157822B2 (en) 2017-05-24 2024-12-03 Poet Research, Inc. Methods of producing vegetable oils with low minerals, metals, or other contaminants
EP3630990A4 (fr) 2017-05-24 2021-03-03 POET Research, Inc. Utilisation d'une estérase pour améliorer la teneur en ester éthylique dans des milieux de fermentation
CN108277212B (zh) * 2017-12-29 2019-12-10 广东溢多利生物科技股份有限公司 脂肪酶突变体Gly183Cys/Gly212Cys及其基因和应用
CN108315312B (zh) * 2018-04-11 2019-12-10 广东溢多利生物科技股份有限公司 一种热稳定性提高的脂肪酶ttl突变体及其编码基因和应用
CA3103242C (fr) 2018-06-11 2023-08-29 Poet Research, Inc. Procedes de raffinage d'une matiere premiere de composition d'huile d'oleagineux, et systemes, compositions et utilisations associes
WO2021045234A1 (fr) * 2019-09-06 2021-03-11 国立大学法人東海国立大学機構 Nouvelle estérase dégradant un ester comprenant un acide gras trans
BR112023001818A2 (pt) 2020-08-06 2023-02-23 Poet Res Inc Lipase endógena para redução de metais em óleo de milho de destilaria
EP4525615A2 (fr) 2022-05-14 2025-03-26 Novozymes A/S Compositions et procédés de prévention, de traitement, de suppression et/ou d'élimination d'infestations et d'infections phytopathogènes

Citations (1)

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Publication number Priority date Publication date Assignee Title
WO2011063402A2 (fr) * 2009-11-23 2011-05-26 Butamax(Tm) Advanced Biofuels Llc Procédé de production du butanol utilisant une fermentation extractive par le biais de l'addition d'un osmolyte

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DE69621765T2 (de) * 1995-09-07 2003-03-06 Kansai Paint Co., Ltd. Kombiniertes Verfahren von Fermentations-und mikrobieller Transformations-Reaktion
US20040253696A1 (en) * 2003-06-10 2004-12-16 Novozymes North America, Inc. Fermentation processes and compositions
WO2008101010A1 (fr) * 2007-02-13 2008-08-21 Water Solutions, Inc. Procédé pour améliorer le rendement et l'efficacité d'une installation de fermentation d'éthanol
WO2009006386A2 (fr) * 2007-06-29 2009-01-08 The Regents Of The University Of California Cellules hôtes et procédés de production d'alcanoates d'isoprényle
AU2009256148B2 (en) * 2008-06-04 2014-11-27 Butamax(Tm) Advanced Biofuels Llc A method for producing butanol using two-phase extractive fermentation
CN102947457B (zh) * 2010-06-18 2016-12-21 布特马斯先进生物燃料有限责任公司 用于在丁醇生产中的提取发酵之前移除不溶解固体的方法和体系

Patent Citations (1)

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Publication number Priority date Publication date Assignee Title
WO2011063402A2 (fr) * 2009-11-23 2011-05-26 Butamax(Tm) Advanced Biofuels Llc Procédé de production du butanol utilisant une fermentation extractive par le biais de l'addition d'un osmolyte

Non-Patent Citations (5)

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Title
HUGHES ET AL: "Production of Candida antarctica lipase B gene open reading frame using automated PCR gene assembly protocol on robotic workcell and expression in an ethanologenic yeast for use as resin-bound biocatalyst in biodiesel production", JALA (JOURNAL OF LABORATORY AUTOMATION), vol. 16, February 2011 (2011-02-01), pages 17 - 37, XP028132864 *
OLIVEIRA ET AL: "In situ recovery of ethanol by extraction and enzymatic esterification", MEDEDELINGEN FACULTEIT LANDBOUWWETENSCHAPPEN RIJKSUNIVERSITEIT GENT, vol. 63/4a, 1998, pages 1231 - 1238, XP002683137 *
OLIVEIRA ET AL: "Production and extractive biocatalysis of ethanol using microencapsulated yeast cells and lipase systems", JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, vol. 52, 1991, pages 219 - 225, XP055029174 *
SRIMHAN ET AL: "Selection of lipase producing yeasts for methanol-tolerant biocatalyst as whole cell application for palm-oil transesterification", ENZYME AND MICROBIAL TECHNOLOGY, vol. 48, 7 March 2011 (2011-03-07), pages 293 - 298, XP028138965 *
YOSHIDA ET AL: "Water activity dependence of performance of surface-displayed lipase in yeast cells: A unique water requirement for enzymatic synthetic reaction in organic media", ENZYME AND MICROBIAL TECHNOLOGY, vol. 48, 7 April 2011 (2011-04-07), pages 334 - 338, XP028372461 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106399286A (zh) * 2016-10-18 2017-02-15 江南大学 一种促进毕赤酵母产碱性果胶酶的方法
CN106399286B (zh) * 2016-10-18 2019-12-24 江南大学 一种促进毕赤酵母产碱性果胶酶的方法

Also Published As

Publication number Publication date
CA2829941A1 (fr) 2012-09-27
JP2014509869A (ja) 2014-04-24
BR112013024329A2 (pt) 2017-12-19
WO2012129548A2 (fr) 2012-09-27
WO2012129548A3 (fr) 2013-03-14
ZA201306647B (en) 2014-11-26
EP2689006A2 (fr) 2014-01-29
AU2012230730A1 (en) 2013-09-19
CN103459587A (zh) 2013-12-18

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Inventor name: KRUCKEBERG, ARTHUR LEO

Inventor name: DICOSIMO, ROBERT

Inventor name: VAN AKEN, THOMAS EDWIN

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