EP2697640A4 - Procédé pour l'établissement du profil de niveaux de phytohormones dans un tissu végétal - Google Patents

Procédé pour l'établissement du profil de niveaux de phytohormones dans un tissu végétal

Info

Publication number
EP2697640A4
EP2697640A4 EP12770990.5A EP12770990A EP2697640A4 EP 2697640 A4 EP2697640 A4 EP 2697640A4 EP 12770990 A EP12770990 A EP 12770990A EP 2697640 A4 EP2697640 A4 EP 2697640A4
Authority
EP
European Patent Office
Prior art keywords
profiling
plant tissue
phytohormone levels
phytohormone
levels
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP12770990.5A
Other languages
German (de)
English (en)
Other versions
EP2697640A1 (fr
Inventor
Regine Fuchs
Martin Dostler
Maria Wahl
Benjamin Hentschel
Ralf Looser
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BASF Plant Science Co GmbH
Original Assignee
BASF Plant Science Co GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BASF Plant Science Co GmbH filed Critical BASF Plant Science Co GmbH
Priority to EP12770990.5A priority Critical patent/EP2697640A4/fr
Publication of EP2697640A1 publication Critical patent/EP2697640A1/fr
Publication of EP2697640A4 publication Critical patent/EP2697640A4/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N2030/009—Extraction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • G01N2030/062—Preparation extracting sample from raw material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/72—Mass spectrometers
    • G01N30/7233—Mass spectrometers interfaced to liquid or supercritical fluid chromatograph
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/72—Mass spectrometers
    • G01N30/7233—Mass spectrometers interfaced to liquid or supercritical fluid chromatograph
    • G01N30/724—Nebulising, aerosol formation or ionisation
    • G01N30/7266—Nebulising, aerosol formation or ionisation by electric field, e.g. electrospray
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/141111—Diverse hetero atoms in same or different rings [e.g., alkaloids, opiates, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/145555—Hetero-N
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/145555—Hetero-N
    • Y10T436/147777—Plural nitrogen in the same ring [e.g., barbituates, creatinine, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/20—Oxygen containing
    • Y10T436/200833—Carbonyl, ether, aldehyde or ketone containing
    • Y10T436/201666—Carboxylic acid
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/24—Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25125—Digestion or removing interfering materials
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
    • Y10T436/255—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.] including use of a solid sorbent, semipermeable membrane, or liquid extraction

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Endocrinology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
EP12770990.5A 2011-04-15 2012-04-13 Procédé pour l'établissement du profil de niveaux de phytohormones dans un tissu végétal Withdrawn EP2697640A4 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP12770990.5A EP2697640A4 (fr) 2011-04-15 2012-04-13 Procédé pour l'établissement du profil de niveaux de phytohormones dans un tissu végétal

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201161475709P 2011-04-15 2011-04-15
EP11162675 2011-04-15
PCT/IB2012/051818 WO2012140603A1 (fr) 2011-04-15 2012-04-13 Procédé pour l'établissement du profil de niveaux de phytohormones dans un tissu végétal
EP12770990.5A EP2697640A4 (fr) 2011-04-15 2012-04-13 Procédé pour l'établissement du profil de niveaux de phytohormones dans un tissu végétal

Publications (2)

Publication Number Publication Date
EP2697640A1 EP2697640A1 (fr) 2014-02-19
EP2697640A4 true EP2697640A4 (fr) 2014-11-12

Family

ID=47008896

Family Applications (1)

Application Number Title Priority Date Filing Date
EP12770990.5A Withdrawn EP2697640A4 (fr) 2011-04-15 2012-04-13 Procédé pour l'établissement du profil de niveaux de phytohormones dans un tissu végétal

Country Status (3)

Country Link
US (1) US9465040B2 (fr)
EP (1) EP2697640A4 (fr)
WO (1) WO2012140603A1 (fr)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103353490B (zh) * 2013-06-24 2015-04-01 浙江大学 一种利用超高效液相色谱电喷雾串联质谱检测梨内源激素的方法
US9625426B2 (en) * 2015-03-04 2017-04-18 Janusz Pawliszyn Standard analyte generator
CN104764827B (zh) * 2015-04-13 2016-10-26 南京信息工程大学 一种番茄植株顶芽内源激素含量的测定方法
CN105557690B (zh) * 2016-01-13 2018-02-23 中国水稻研究所 一种低氧逆境下的水稻种子引发剂及其使用方法
CN106404962B (zh) * 2016-11-30 2018-06-29 山东省花生研究所 一种测定花生叶片茉莉酸含量的方法
CN109212052B (zh) * 2018-07-07 2021-05-11 丁立平 一种测定水源水中五种痕量吲哚羧酸类化合物的气相色谱-质谱联用法
CN109212057B (zh) * 2018-08-19 2021-05-11 丁立平 一种测定水源水中五种痕量植物生长调节剂的气相色谱-质谱联用法
CN109324139A (zh) * 2018-12-14 2019-02-12 广西中烟工业有限责任公司 一种烟叶中玉米素核苷液液萃取-液相色谱-串联质谱的测定方法
CN111308006B (zh) * 2020-03-16 2022-12-13 上海中科新生命生物科技有限公司 一种基于lc-ms高通量高灵敏度检测植物激素的方法
CN111323506B (zh) * 2020-03-23 2023-01-17 湖南农业大学 一种高油脂植物样品中植物激素测定的方法
CN113061070A (zh) * 2021-03-29 2021-07-02 阿尔塔(天津)标准物质研究院有限公司 一种氘标记的美替诺龙稳定性同位素标记化合物
CN115856174A (zh) * 2022-12-05 2023-03-28 南京医科大学 急性主动脉夹层血浆生物标志物及其应用
CN119375408B (zh) * 2024-12-30 2025-03-25 内蒙古农业大学 一种测定酵母培养物中n-乙酰谷氨酸含量的方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012035117A1 (fr) * 2010-09-15 2012-03-22 Basf Plant Science Company Gmbh Dispositif d'extraction et procédé d'extraction d'ingrédients à partir d'un échantillon biologique

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012035117A1 (fr) * 2010-09-15 2012-03-22 Basf Plant Science Company Gmbh Dispositif d'extraction et procédé d'extraction d'ingrédients à partir d'un échantillon biologique

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
DOBREV P I ET AL: "Purification and determination of plant hormones auxin and abscisic acid using solid phase extraction and two-dimensional high performance liquid chromatography.", JOURNAL OF CHROMATOGRAPHY. A 20 MAY 2005 LNKD- PUBMED:15974129, vol. 1075, no. 1-2, 20 May 2005 (2005-05-20), pages 159 - 166, XP002645665, ISSN: 0021-9673 *
DOBREV PETRE IVANOV ET AL: "Fast and efficient separation of cytokinins from auxin and abscisic acid and their purification using mixed-mode solid-phase extraction", JOURNAL OF CHROMATOGRAPHY A, vol. 950, no. 1-2, 15 March 2002 (2002-03-15), pages 21 - 29, XP002645664, ISSN: 0021-9673 *
HONG-TAO LIU ET AL: "Simultaneous Determination of Phytohormones in Plant Extracts using SPME and HPLC", CHROMATOGRAPHIA ; AN INTERNATIONAL JOURNAL FOR RAPID COMMUNICATION IN CHROMATOGRAPHY, ELECTROPHORESIS AND ASSOCIATED TECHNIQUES, VIEWEG VERLAG, WI, vol. 66, no. 7-8, 1 September 2007 (2007-09-01), pages 515 - 520, XP019522200, ISSN: 1612-1112, DOI: 10.1365/S10337-007-0350-3 *
HOU S ET AL: "Simultaneous determination of gibberellic acid, indole-3-acetic acid and abscisic acid in wheat extracts by solid-phase extraction and liquid chromatography-electrospray tandem mass spectrometry", TALANTA, ELSEVIER, AMSTERDAM, NL, vol. 76, no. 4, 15 August 2008 (2008-08-15), pages 798 - 802, XP023178158, ISSN: 0039-9140, [retrieved on 20080424], DOI: 10.1016/J.TALANTA.2008.04.041 *
PAN X ET AL: "Simultaneous quantification of major phytohormones and related compounds in crude plant extracts by liquid chromatography-electrosp ray tandem mass spectrometry", PHYTOCHEMISTRY, PERGAMON PRESS, GB, vol. 69, no. 8, 1 May 2008 (2008-05-01), pages 1773 - 1781, XP022639379, ISSN: 0031-9422, [retrieved on 20080325], DOI: 10.1016/J.PHYTOCHEM.2008.02.008 *
See also references of WO2012140603A1 *

Also Published As

Publication number Publication date
EP2697640A1 (fr) 2014-02-19
WO2012140603A1 (fr) 2012-10-18
US9465040B2 (en) 2016-10-11
US20140147925A1 (en) 2014-05-29

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