EP3148480B1 - System zur erhaltung einer vordefinierten dosis einer flüssigen substanz, insbesondere von verdünntem tiersamen - Google Patents

System zur erhaltung einer vordefinierten dosis einer flüssigen substanz, insbesondere von verdünntem tiersamen Download PDF

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Publication number
EP3148480B1
EP3148480B1 EP15732810.5A EP15732810A EP3148480B1 EP 3148480 B1 EP3148480 B1 EP 3148480B1 EP 15732810 A EP15732810 A EP 15732810A EP 3148480 B1 EP3148480 B1 EP 3148480B1
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EP
European Patent Office
Prior art keywords
stopper
tube
liquid
cations
straw
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
EP15732810.5A
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English (en)
French (fr)
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EP3148480A1 (de
Inventor
Eric Schmitt
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IMV Technologies SA
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IMV Technologies SA
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Publication of EP3148480A1 publication Critical patent/EP3148480A1/de
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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00—Instruments or methods for reproduction or fertilisation
    • A61D19/02—Instruments or methods for reproduction or fertilisation for artificial insemination
    • A61D19/022—Containers for animal semen, e.g. pouches or vials ; Methods or apparatus for treating or handling animal semen containers, e.g. filling or closing
    • A61D19/024—Tube-like containers, e.g. straws
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A01N1/14—Mechanical aspects of preservation; Apparatus or containers therefor
    • A01N1/146—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving
    • A01N1/147—Carriers for immersion in cryogenic fluid for slow freezing or vitrification
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00—Instruments or methods for reproduction or fertilisation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00—Instruments or methods for reproduction or fertilisation
    • A61D19/02—Instruments or methods for reproduction or fertilisation for artificial insemination
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00—Instruments or methods for reproduction or fertilisation
    • A61D19/04—Instruments or methods for reproduction or fertilisation for embryo transplantation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48—Reproductive organs
    • A61K35/52—Sperm; Prostate; Seminal fluid; Leydig cells of testes

Definitions

  • the invention relates generally to the storage of a predetermined dose of liquid-based substance containing biological material.
  • a substance is, for example, diluted animal semen.
  • the invention relates more particularly to flakes for carrying out such preservation.
  • these flakes are conventionally formed by a thin tube, having for example an internal diameter of 1.6 or 2.5 mm, and by a stopper engaged in the thin tube.
  • the stopper In the filled state, the stopper is placed in the vicinity of a first end of the tube and the dose of liquid-based substance is placed in the straw between the stopper and the second end of the tube.
  • the first end of the tube close to the stopper, is placed in communication with a vacuum source while the second end is placed in communication with a container containing the substance to be introduced into the straw.
  • the air initially contained between the stopper and the second end is drawn through the stopper as the substance progresses through the tube until it meets the stopper, which it cannot pass because it becomes liquid tight.
  • the straw is welded in the vicinity of one or both of its ends and is stored cold.
  • a rod is made to penetrate into the tube through the end closest to the stopper, which bears against the stopper. With this rod, the stopper is made to slide like a piston towards the end furthest from the stopper, so that the dose of substance initially contained in the straw is expelled from this end.
  • the straw caps are of the tripartite type originally described in the French patent. 995,878 , corresponding to the British patent 669,265 , that is to say formed by two pads of fibrous substance enclosing a powder which transforms on contact with a liquid into an impermeable paste or gel adhering to the wall of the tube so that the stopper is liquid-tight.
  • the European patent application EP 0 873 726 proposes that the stopper be made of a one-piece cylinder of hydrophobic microporous material.
  • the stopper consists of a rigid insert pierced with a substantially coaxial orifice and a microporous and hydrophobic membrane associated with the insert to close the orifice of the insert on the internal side.
  • the document FR 2 810 535 discloses a method of filling a storage straw with semen comprising a plug placed between two pads.
  • a diluter that is to say a non-spermicidal and non-adherent liquid medium, ensuring the preservation and / or nutrition of the semen.
  • diluents are based on citrate, egg yolk and glycerol. Where appropriate, it may also contain calcium ions capable of gelling the powder of the stopper more rapidly when it is alginate. 11
  • the invention aims to limit the loss, in the stopper of such a straw, of the product of interest contained in the liquid-based substance, for example spermatozoa if the substance is diluted semen.
  • the primary product contained in the dilution medium is an alginate in solution
  • the reagent contained in the stopper of the straw is formed by multivalent cations.
  • the liquid-based substance contains an alginate
  • the substance thickens and forms a matrix or a network which cannot be crossed by the product of interest, for example. example sperm, which therefore cannot enter the plug where it would be lost.
  • the system according to the invention therefore makes it possible to limit the loss of the product of interest in the stopper of the straw.
  • said liquid-based substance is diluted animal semen and said alginate is in a concentration of between 0.1 and 6 g / l; and even more advantageously in a concentration of between 2.5 and 5 g / l.
  • the hydrogel formed is likely to be different from a matrix or multivalent alginate-cation network.
  • the primary product contained in the dilution medium is different from alginates, for example a pectin, and / or the reagent contained in the stopper of the straw is different from multivalent cations, for example an acid.
  • the straw 10 illustrated on figure 1 comprises a tube 11 and a stopper 12.
  • the tube 11 is conventionally made of extruded plastic material, here transparent, with an internal diameter which is for example 1.6 or 2.5 mm and a length of the order of 133 mm.
  • the plug 12 is of the tripartite type, that is to say formed by two plugs 13 and 14 of a fibrous substance, for example in braided threads, enclosing a sealing agent 20 formed by a powder 15 ( figure 1 ) capable of transforming on contact with a liquid into a 15 'paste or gel ( figure 2 ) waterproof adhering to the wall of the tube 11 so that the stopper 12 is liquid tight.
  • the stopper 12 is arranged in the vicinity of the end 16 of the tube 11 and it is provided that in the filled state, the dose of liquid-based substance which must be kept in the straw 10 is placed between the stopper 12 and the end 17 of the tube 11 furthest from the stopper 12.
  • the end 16 is placed in communication with a vacuum source while the end 17 is placed in communication with a container containing the substance to be introduced into the straw.
  • the straw 10 is then in the filled state shown on figure 2 .
  • the straw is welded in the vicinity of one or both of its ends 16 and 17 and is stored cold.
  • the stopper 12 is slid in the manner of a piston towards the end 17 or the corresponding end after cutting the welded portion, which causes the expulsion of the dose of substance 21 which had been introduced. in the straw.
  • the buffer 14 is impregnated with bivalent cations 22, here cations of barium Ba ++ .
  • Substance 21 is here diluted animal semen which contains an alginate 23.
  • divalent cations such as cations 22 have the capacity to crosslink alginates such as alginate 23.
  • the matrix or network 24 is such that the spermatozoa 25 contained in the substance 21 cannot cross the matrix or network 24.
  • the buffer 14 is entirely impregnated with divalent cations 22, for reasons of manufacturing convenience.
  • the pad 14 is made from a section of a braided wick which has been soaked in a saturated solution of barium salt then removed from the bath and allowed to dry before being cut into sections, one of which is forms the buffer 14.
  • the figure 4 shows a flask 26 containing a dilution medium 27, a container 28 containing pure animal semen 29 and the same container 28 containing diluted animal semen, forming substance 21, obtained by diluting pure animal semen 29 with the medium dilution 27.
  • the dilution medium 27 has not yet been mixed with pure seed, and is therefore distinct from the diluted seed.
  • the dilution medium 27 is for example that marketed by the applicant under the name OptiXcell.
  • the dilution of the pure semen 29 by the medium 27 is carried out under predetermined conditions, in particular as regards the dilution ratio, that is to say the ratio between the total volume of the diluted semen 21 and the volume of the pure seed 29.
  • the dilution medium 27 contains the alginate 23, which has been selected to crosslink in contact with the divalent cations 22 in order to form the matrix or network 24, which is therefore a thickened zone of the diluted seed 21.
  • a particularly suitable concentration range is the range from 0.1 g to 6 g of alginate per liter of dilution medium 27.
  • a particularly advantageous range is between 2.5 g / l and 5 g / l. .
  • the proportion of liquid-based substance lost in the stopper 12 is 3 to 4%; and with a concentration of the order of 5 g / l, the proportion of liquid-based substance lost in the stopper such as 12 is of the order of 1 to 3%.
  • this proportion of liquid lost in the stopper is determined by making the difference between the quantity of liquid-based substance introduced into the straw until the stopper 12 becomes liquid-tight and the quantity of liquid that can be released by the straw. straw by sliding the cap 12 as explained above.
  • the matrix or network can extend to a relatively large distance from the end 18 of the plug 12.
  • the variant of the straw 10 shown on figure 5 is similar to the sequin 10 shown on the figures 1 to 3 except that the stopper 12 also includes a barrier pad 30 on the side of the end 17 of the tube 11.
  • the barrier pad 30 extends between an end 31 facing the end 17 of the tube 11 and an end 32 facing the end 16 of the tube 11.
  • the end 18 of the stopper 12 turned towards the end 17 of the tube 11 forms part of the buffer 14.
  • the end 18 forms part of the barrier buffer 30 and the buffer 14 has, on the side facing the end 17, an end 33 separate from the end 18.
  • the end 33 of the buffer 14 and the end 32 of the barrier buffer 30 are disposed against each other.
  • the barrier pad 30 is fibrous. It is permeable to gases and liquids.
  • the sequin 10 shown on the figure 5 is used in the same way as the straw 10 shown on the figures 1 to 3 .
  • the substance 21 passes through the barrier pad 30 and meets the pad 14 by its end 33 facing the end 17 of the tube 11.
  • the substance 21 thickens and the matrix or network 24 is formed.
  • the barrier buffer 30 allows the matrix or network 24 to remain in the plug 12: the barrier buffer 30 prevents the passage of the matrix or network 24 towards the substance 21.
  • the barrier pad 30 is here a braid formed by son arranged in a core and a covering surrounding the core.
  • Each wire is hydrophobic. Therefore, the barrier pad 30 is hydrophobic, and therefore has a water repellant effect.
  • This repellent effect does not prevent the substance 21 from passing through the barrier pad 30 and reaching the impregnated pad 14, since in practice the substance 21 meets the plug 12 with a certain speed.
  • the barrier pad 30 does not keep the liquid located in its interstices but pushes it back into the dose of liquid substance located between the end 31 of the barrier pad 30 and the end 17 of tube 11.
  • the tripartite plug 12 is replaced by a plug formed only by a buffer such as buffer 14.
  • the stopper 12 is arranged differently, for example made of a monobloc cylinder of hydrophobic microporous material as described in the European patent application EP 0 873 726 .
  • bivalent cations 22 formed by barium Ba ++ ions are replaced by other bivalent cations, for example calcium Ca ++ or magnesium Mg ++ cations.
  • the bivalent cations 22 are replaced by multivalent cations other than bivalent, for example trivalent cations such as aluminum cations Al +++ or chromium Cr +++ .
  • the matrix or network 24 alginate - multivalent ions is replaced by another hydrogel, that is to say a network or matrix of polymer chains swollen with a liquid-based substance, for example a matrix or pectin network - multivalent cations (the alginate of the dilution medium is replaced by a pectin); and / or the reagent impregnating an element of the stopper is different from multivalent cations, for example an acid.
  • liquid-based substance containing biological material is different from diluted animal semen, for example a storage medium containing embryos.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Reproductive Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Transplantation (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Claims (14)

  1. System zur Aufbewahrung von biologischem Material, dadurch gekennzeichnet, dass es umfasst:
    - ein Röhrchen (10) zur Aufbewahrung einer vorbestimmten Dosis einer Substanz auf Flüssigkeitsbasis, die das biologische Material enthält, das ein Rohrstück (11) umfasst, das sich zwischen einem ersten Ende (16) und einem zweiten Ende (17) erstreckt und einen Stopfen (12) umfasst, der gasdurchlässig und flüssigkeitsdicht ist, wobei der Stopfen (12) in dem Rohrstück (11) in der Nähe seines ersten Endes (16) angeordnet ist und sich zwischen einem ersten Ende (19), das dem ersten Ende (16) des Rohrstücks (11) zugewandt ist, und einem zweiten Ende (18), das dem zweiten Ende (17) des Rohrstücks (11) zugewandt ist, erstreckt; wobei der Stopfen (12) im anfänglich leeren Zustand des Röhrchens (10) ein Element (14) umfasst, das zumindest in der Nähe eines ersten Endes (18; 33), das dem zweiten Ende (17) des Rohrstücks (11) zugewandt ist, mit einem Reagens (22) imprägniert ist; und
    - ein flüssiges Verdünnungsmedium (27), um durch Mischen unter vorbestimmten Bedingungen die Substanz auf Flüssigkeitsbasis (21) zu ergeben;
    wobei das Reagens (22) und das flüssige Verdünnungsmedium (27) derart ausgestaltet sind, dass sich bei Kontakt der Substanz (21) mit dem ersten Ende (18; 33) des imprägnierten Elements (14) ein Hydrogel (24) bildet.
  2. System nach Anspruch 1, dadurch gekennzeichnet, dass das Reagens durch mehrwertige Kationen gebildet ist.
  3. System nach Anspruch 2, dadurch gekennzeichnet, dass die mehrwertigen Kationen zweiwertige Kationen sind.
  4. System nach Anspruch 3, dadurch gekennzeichnet, dass die zweiwertigen Kationen Bariumkationen Ba++ (22) umfassen.
  5. System nach Anspruch 3, dadurch gekennzeichnet, dass die zweiwertigen Kationen Calciumkationen Ca++ umfassen.
  6. System nach nach einem der Ansprüche 1 bis 5, dadurch gekennzeichnet, dass der Stopfen (12) zwei Pfropfen (13, 14) aus faserartigem Material umfasst, die ein Dichtungsmittel (20) einschließen, das aus einem Pulver (15) besteht, das sich bei Kontakt mit einer Flüssigkeit in eine undurchlässige Paste oder ein undurchlässiges Gel (15') verwandelt, das an der Wand des Rohrstücks haftet, so dass der Stopfen flüssigkeitsdicht ist.
  7. System nach Anspruch 6, dadurch gekennzeichnet, dass in dem Stopfen (12) des Röhrchens (10) der Pfropfen (14), der auf der Seite des zweiten Endes (17) des Rohrstücks (11) gelegen ist, das imprägnierte Element bildet, wobei das erste Ende (18) des imprägnierten Elements das zweite Ende des Stopfens bildet.
  8. System nach einem der Ansprüche 1 bis 6, dadurch gekennzeichnet, dass der Stopfen (12) einen gas- und flüssigkeitsdurchlässigen Sperrpfropfen (30) umfasst, der sich zwischen einem ersten Ende (31), das dem ersten Ende (17) des Rohrstücks (11) zugewandt ist, und einem zweiten Ende (32), das dem zweiten Ende (16) des Rohrstücks (11) zugewandt ist, erstreckt, wobei das erste Ende (33) des imprägnierten Elements (14) und das zweite Ende (32) des Sperrpfropfens (30) gegeneinander angeordnet sind.
  9. System nach Anspruch 8, dadurch gekennzeichnet, dass der Sperrpfropfen (30) hydrophob ist.
  10. System nach einem der Ansprüche 1 bis 9, dadurch gekennzeichnet, dass das imprägnierte Element ein Pfropfen (14) ist, der vollständig mit bivalenten Kationen (22) imprägniert ist.
  11. System nach einem der Ansprüche 1 bis 10, dadurch gekennzeichnet, dass das flüssige Verdünnungsmedium (27) ein Alginat (23) in Lösung enthält.
  12. System nach Anspruch 11, dadurch gekennzeichnet, dass die Substanz auf Flüssigkeitsbasis verdünntes Tiersperma ist und das Alginat in einer Konzentration zwischen 0,1 und 6 g/l vorliegt.
  13. System nach Anspruch 12, dadurch gekennzeichnet, dass das Alginat in einer Konzentration zwischen 2,5 und 5 g/l vorliegt.
  14. System nach einem der Ansprüche 1 bis 13, dadurch gekennzeichnet, dass das Hydrogel eine/ein Alginat-mehrwertige-Kationen-Matrize oder -Gitter (24) ist.
EP15732810.5A 2014-05-28 2015-05-27 System zur erhaltung einer vordefinierten dosis einer flüssigen substanz, insbesondere von verdünntem tiersamen Active EP3148480B1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR1454894A FR3021522A1 (fr) 2014-05-28 2014-05-28 Paillette pour la conservation d'une dose predeterminee de substance a base liquide, notamment de la semence animale diluee ; milieu de dilution pour donner une telle substance ; et systeme les comportant
PCT/FR2015/051396 WO2015181496A1 (fr) 2014-05-28 2015-05-27 Système pour la conservation d'une dose prédéterminée de substance à base liquide, notamment de la semence animale diluée

Publications (2)

Publication Number Publication Date
EP3148480A1 EP3148480A1 (de) 2017-04-05
EP3148480B1 true EP3148480B1 (de) 2021-02-24

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Family Applications (2)

Application Number Title Priority Date Filing Date
EP15732809.7A Active EP3148479B1 (de) 2014-05-28 2015-05-27 Pipette zur beibehaltung einer vorbestimmten dosis einer flüssigen substanz, insbesondere verdünntes tiersperma, und system mit solch einer pipette und einem verdünnungsmedium zur herstellung solch einer substanz
EP15732810.5A Active EP3148480B1 (de) 2014-05-28 2015-05-27 System zur erhaltung einer vordefinierten dosis einer flüssigen substanz, insbesondere von verdünntem tiersamen

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Application Number Title Priority Date Filing Date
EP15732809.7A Active EP3148479B1 (de) 2014-05-28 2015-05-27 Pipette zur beibehaltung einer vorbestimmten dosis einer flüssigen substanz, insbesondere verdünntes tiersperma, und system mit solch einer pipette und einem verdünnungsmedium zur herstellung solch einer substanz

Country Status (5)

Country Link
US (2) US20170196669A1 (de)
EP (2) EP3148479B1 (de)
DK (2) DK3148480T3 (de)
FR (2) FR3021522A1 (de)
WO (2) WO2015181495A1 (de)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018104160A1 (en) 2016-12-05 2018-06-14 Spermvital As Sustained release composition
CN112367841B (zh) * 2018-06-04 2023-06-09 斯博姆维透公司 用于制备水凝胶的功能化试剂盒

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FR2753367B1 (fr) * 1996-09-19 1998-12-04 Lecointe Pascal Paillette de stockage d'une substance biologique liquide et son procede de remplissage
FR2762210B1 (fr) 1997-04-21 1999-07-02 Instr Medecine Veterinaire Paillette avec un bouchon en fibre pleine microporeuse
IT1292275B1 (it) 1997-04-23 1999-01-29 Comau Spa Polso di robot industriale, e robot comprendente tale polso.
FR2771285B1 (fr) 1997-11-24 2000-02-11 Instr Medecine Veterinaire Paillette avec bouchon bipartite
IT1296585B1 (it) * 1997-11-28 1999-07-14 Uni Di Pavia Microcapsule contenenti materiale seminale per l'inseminazione strumentale nella specie suina
FR2784572B1 (fr) 1998-10-15 2001-02-09 Instr Medecine Veterinaire Procede de fabrication d'un element constitue d'un support et d'une membrane qui y est soudee, en particulier d'un bouchon de paillette, et ensemble membrane support obtenu par ce procede
FR2810535B1 (fr) * 2000-06-27 2002-10-11 Eurl Cryo Vet Procede de remplissage d'une paillette de stockage avec de la semence
JP3848064B2 (ja) * 2000-08-07 2006-11-22 シャープ株式会社 半導体不揮発性メモリの試験方法
FR2824255B1 (fr) 2001-05-07 2004-02-06 Imv Technologies Paillette pour la conservation de petites quantites de substances, notamment de liquides biologiques
FR2824256B1 (fr) 2001-05-07 2004-04-02 Imv Technologies Paillette pour la conservation de petites quantites de substances, en particulier de liquides biologiques
ITMI20050552A1 (it) * 2005-04-04 2006-10-05 Univ Degli Studi Milano CAPSULE CONTENENTI MATERIAlE SEMINALE PER INSEMINAZIONE ARTIFICIALE
US20090020856A1 (en) * 2007-07-17 2009-01-22 International Business Machines Corporation Semiconductor device structures and methods for shielding a bond pad from electrical noise
ES2399933B1 (es) * 2011-05-24 2014-03-26 Javier Gil Pascual Diluyente para suspension seminal
GB201120368D0 (en) * 2011-11-24 2012-01-04 Sperm Vital As Methods for the preparation of hydrogels

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Also Published As

Publication number Publication date
WO2015181495A1 (fr) 2015-12-03
FR3021523A1 (fr) 2015-12-04
EP3148479B1 (de) 2020-09-16
US10278798B2 (en) 2019-05-07
DK3148479T3 (da) 2020-12-21
FR3021523B1 (fr) 2021-02-26
FR3021522A1 (fr) 2015-12-04
US20170196669A1 (en) 2017-07-13
DK3148480T3 (da) 2021-05-25
EP3148480A1 (de) 2017-04-05
WO2015181496A1 (fr) 2015-12-03
US20170189156A1 (en) 2017-07-06
EP3148479A1 (de) 2017-04-05

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