EP3242133A4 - Zusammensetzung und system zur trennung und detektion von alpha-fetoproteinvarianten und verwendung davon - Google Patents

Zusammensetzung und system zur trennung und detektion von alpha-fetoproteinvarianten und verwendung davon Download PDF

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Publication number
EP3242133A4
EP3242133A4 EP15875252.7A EP15875252A EP3242133A4 EP 3242133 A4 EP3242133 A4 EP 3242133A4 EP 15875252 A EP15875252 A EP 15875252A EP 3242133 A4 EP3242133 A4 EP 3242133A4
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Prior art keywords
separating
composition
detecting alpha
fetoprotein variant
fetoprotein
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EP15875252.7A
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French (fr)
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EP3242133A1 (de
EP3242133B1 (de
Inventor
Haifeng LUO
Boan Li
Changqing Lin
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BEIJING HOTGEN BIOTECH CO Ltd
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BEIJING HOTGEN BIOTECH CO Ltd
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4715—Pregnancy proteins, e.g. placenta proteins, alpha-feto-protein, pregnancy specific beta glycoprotein
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/1703—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • A61K38/1709—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/575—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57525—Immunoassay; Biospecific binding assay; Materials therefor for cancer of the liver or pancreas
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • G01N2030/065—Preparation using different phases to separate parts of sample

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Organic Chemistry (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Genetics & Genomics (AREA)
  • Gynecology & Obstetrics (AREA)
  • Biophysics (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
EP15875252.7A 2014-12-31 2015-12-30 Zusammensetzung und system zur trennung und detektion von alpha-fetoproteinvarianten und verwendung davon Active EP3242133B1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201410841397 2014-12-31
PCT/CN2015/099774 WO2016107576A1 (zh) 2014-12-31 2015-12-30 一种甲胎蛋白异质体的分离检测组合物、系统及其应用

Publications (3)

Publication Number Publication Date
EP3242133A4 true EP3242133A4 (de) 2017-11-08
EP3242133A1 EP3242133A1 (de) 2017-11-08
EP3242133B1 EP3242133B1 (de) 2020-03-18

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EP15875252.7A Active EP3242133B1 (de) 2014-12-31 2015-12-30 Zusammensetzung und system zur trennung und detektion von alpha-fetoproteinvarianten und verwendung davon

Country Status (6)

Country Link
US (1) US10329335B2 (de)
EP (1) EP3242133B1 (de)
JP (1) JP6655622B2 (de)
CN (1) CN104714026B (de)
PT (1) PT3242133T (de)
WO (1) WO2016107576A1 (de)

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CN105785043B (zh) * 2016-04-06 2018-02-02 上海良润生物医药科技有限公司 用于定量检测afp‑l3%的试剂盒
CN106198998A (zh) * 2016-06-30 2016-12-07 深圳市亚辉龙生物科技股份有限公司 人甲胎蛋白异质体3化学发光免疫检测试剂盒及其制备方法
CN105974132A (zh) * 2016-07-04 2016-09-28 福建广生堂药业股份有限公司 一种甲胎蛋白异质体的检测方法、检测试剂和检测试剂盒
CN106248959B (zh) * 2016-07-21 2019-01-25 首都医科大学附属北京佑安医院 一种检测血清岩藻糖蛋白的免疫渗滤方法及免疫渗滤装置
CN108333343A (zh) * 2017-01-19 2018-07-27 深圳市新产业生物医学工程股份有限公司 免疫磁性复合物、其制备方法、包括其的抗原捕获剂、试剂盒、系统及应用
CN108333361A (zh) * 2017-01-19 2018-07-27 深圳市新产业生物医学工程股份有限公司 桥连有抗体的磁性微球复合制剂及其制备方法与应用
CN108196063B (zh) * 2017-12-26 2020-11-24 陈复华 一种功能磁球主导的免疫荧光试纸条定量检测甲胎蛋白变异体（afp-l3）的试剂盒
CN108761088B (zh) * 2018-06-28 2021-01-05 北京热景生物技术股份有限公司 用于糖链异常蛋白分离检测的组合物、试剂盒以及方法和用途
CN108627653B (zh) * 2018-06-28 2021-01-05 热景（廊坊）生物技术有限公司 用于甲胎蛋白异质体分离检测的组合物和试剂盒
CN109187983B (zh) * 2018-08-10 2021-10-26 杭州市妇产科医院 中孕期母血清甲胎蛋白异质体l2和l3筛查胎儿开放性神经管畸形的方法
CN110007023B (zh) * 2019-04-15 2021-08-13 陕西科技大学 鱼体内磺胺类药物的高分辨质谱筛查方法及其与蛋白大分子相互作用的分析方法
CN110320370B (zh) * 2019-07-31 2023-06-09 万东山 肝癌肿瘤标志物afp、甲胎蛋白异质体、gp73抗原同步免疫检测试剂盒、方法及应用
CN111253491B (zh) * 2020-01-19 2022-03-25 北京尧景基因技术有限公司 一种用于分离临床样本中的糖基化外泌体的凝集素-磁性载体偶联复合物
CN113433318A (zh) * 2021-06-29 2021-09-24 山东中鸿特检生物科技有限公司 一种检测甲胎蛋白异质体afp-l3含量的试剂盒及其检测方法和应用
CN114414802A (zh) * 2021-09-23 2022-04-29 湖南莱拓福生物科技有限公司 一种基于肝癌四项联合检测的早期诊断试剂盒及应用
CN114487385A (zh) * 2021-12-29 2022-05-13 深圳市国赛生物技术有限公司 甲胎蛋白异质体检测组合物及其制备方法和应用
CN114324340B (zh) * 2022-01-20 2023-08-15 青岛市妇女儿童医院 一种医用血液甲胎蛋白检测装置
CN114544931B (zh) * 2022-02-28 2025-07-18 河南美凯生物科技有限公司 一种干式化学发光法的检测系统
CN114740203B (zh) * 2022-04-13 2025-06-27 中国医科大学附属盛京医院 基于特定凝集素组合与甲胎蛋白构建肝癌鉴别工具的应用

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XIAO-FENG ZHANG ET AL: "Lens Culinaris Agglutinin-Reactive Fraction of Alpha-Fetoprotein as a Marker of Prognosis and a Monitor of Recurrence of Hepatocellular Carcinoma After Curative Liver Resection", ANNALS OF SURGICAL ONCOLOGY, SPRINGER-VERLAG, NE, vol. 18, no. 8, 20 February 2011 (2011-02-20), pages 2218 - 2223, XP019926940, ISSN: 1534-4681, DOI: 10.1245/S10434-011-1613-7 *
ZHANG Q -Y ET AL: "Comparison of chemiluminescence enzyme immunoassay based on magnetic microparticles with traditional colorimetric ELISA for the detection of serum [alpha]-fetoprotein", JOURNAL OF PHARMACEUTICAL ANALYSIS 2012 XI'AN JIAOTONG UNIVERSITY CHN, vol. 2, no. 2, April 2012 (2012-04-01), pages 130 - 135, XP002772916, ISSN: 2095-1779 *
ZHOU HANKUN ET AL: "The sandwich-type electrochemiluminescence immunosensor for alpha-fetoprotein based on enrichment by Fe3O4-Au magnetic nano probes and signal amplification by CdS-Au composite nanoparticles labeled anti-AFP", ANALYTICA CHIMICA ACTA, vol. 746, October 2012 (2012-10-01), pages 107 - 113, XP002772915 *

Also Published As

Publication number Publication date
PT3242133T (pt) 2020-06-19
US10329335B2 (en) 2019-06-25
JP2018502297A (ja) 2018-01-25
JP6655622B2 (ja) 2020-02-26
US20180009863A1 (en) 2018-01-11
CN104714026A (zh) 2015-06-17
WO2016107576A1 (zh) 2016-07-07
EP3242133A1 (de) 2017-11-08
EP3242133B1 (de) 2020-03-18
CN104714026B (zh) 2018-08-21

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