EP3350301A1 - Nanoperles fonctionnalisées par des enzymes destinées à être utilisées pour lutter contre l'encrassement biologique - Google Patents

Nanoperles fonctionnalisées par des enzymes destinées à être utilisées pour lutter contre l'encrassement biologique

Info

Publication number
EP3350301A1
EP3350301A1 EP16778221.8A EP16778221A EP3350301A1 EP 3350301 A1 EP3350301 A1 EP 3350301A1 EP 16778221 A EP16778221 A EP 16778221A EP 3350301 A1 EP3350301 A1 EP 3350301A1
Authority
EP
European Patent Office
Prior art keywords
silica
nanobead
composition
nanobeads
functionalised
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP16778221.8A
Other languages
German (de)
English (en)
Inventor
Michelle ZANONI
Jessica Amadio
Olivier HABIMANA
Eoin Casey
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University College Dublin
Original Assignee
University College Dublin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University College Dublin filed Critical University College Dublin
Priority claimed from PCT/EP2016/071946 external-priority patent/WO2017046313A1/fr
Publication of EP3350301A1 publication Critical patent/EP3350301A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/02—Inorganic compounds ; Elemental compounds
    • C11D3/12—Water-insoluble compounds
    • C11D3/124—Silicon containing, e.g. silica, silex, quartz or glass beads
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • A01N37/46—N-acyl derivatives
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D17/00—Detergent materials or soaps characterised by their shape or physical properties
    • C11D17/0039—Coated compositions or coated components in the compositions, (micro)capsules
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38681—Chemically modified or immobilised enzymes
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/48—Medical, disinfecting agents, disinfecting, antibacterial, germicidal or antimicrobial compositions

Definitions

  • the invention relates to enzyme-functionalised nanobeads for anti-biofouling purposes and methods.
  • the invention relates to enzyme-functionalised silicone nanobeads for anti-biofouling and methods thereof.
  • biofouling in desalination facilities are estimated to cost €12 billion per annum; biofouling on crude oil distillation alone is estimated to cost €3.5 billion per annum worldwide; in aquaculture an effective solution would result in savings of €130- 260 million per annum, 5-10% of market value, and in one case study the cost of biofouling in a single water treatment works was estimated at €600,000 per annum.
  • WO 2009/062518 discloses an anti-fouling composition comprising one or more aerogels, which encapsulate one or more bioactive agents.
  • the agents which may comprise enzymes, can be released from the aerogel over time.
  • the aerogel may be made from silicates.
  • This invention relates to the development of reusable enzyme-functionalized nano-beads as anti-fouling agents. Specifically it relates to the problem of biofouling, which is caused by the formation of biofilms (fouling layers) in aqueous environments of relevance to the process and healthcare industries.
  • the nanobeads surface will be functionalised with active enzymes ⁇ i.e. proteases, polysaccharidases, DNAases).
  • the nanobeads with antifouling properties are suspended in the water, which is in contact with the biofilm.
  • the antifouling property will be optimized with respect to bead size, shape and type, as well as type of enzymes.
  • tetraethyl orthosilicate tetramethyl orthosilicate
  • tetramethyl orthosilicate which can also be synthesised by different methods, such as, but not limited to, the Stoeber, reverse microemulsion, direct micelle assisted methods, which are well-known to those skilled in the art.
  • their physical configuration i.e. spherical, cylindrical, or porous
  • can also be modified significantly by changing the preparation conditions of the reaction site i.e. pH, concentration of surfactant and concentration of starting reagents).
  • the silica-based nanobead is comprised of silicon, and an organic- based linker composed by carbon, oxygen and nitrogen.
  • the silica-based nanobead consists of silicon, carbon, oxygen and nitrogen.
  • the silica-based nanobead is spherical or substantially spherical.
  • the second moiety is a protease selected from Proteinase K, papain, pepsin, trypsin; an oxidoreductase selected from peroxidases, alcohol oxidoreductases, aldehyde oxidoreductases and monooxygenases; a lipase selected from bile salt-dependent lipase, phospholipase A and C; a polysaccharidase carbohydrase and a DNAse (like nucleases).
  • the second moiety is selected from a protease or an oxidoreductase.
  • the protease is Proteinase K and the oxidoreductase is a peroxidase.
  • the second moiety is Proteinase K.
  • the recycled silica-based nanobead composition is reintroduced to the addition step.
  • nanobeads should be understood to mean beads between 40 and 950 nanometres in size.
  • the term “functionalized” should be understood to mean a variety of active units, such as peptides or enzymes or chemically active groups, adhered to a support frame.
  • the support frame is a silica-based nanobead.
  • fouling layer should be understood to encompass a variety of fouling layers, such as biofilm fouling layer, an organic fouling layer, and a colloidal fouling layer.
  • biofilm fouling layer should be understood to mean a multidimensional micro-ecosystem composed of aggregated microorganisms attached on either a biotic or abiotic surface and immobilized by a self-produced matrix of extracellular polymeric substance.
  • organic fouling layer should be understood to mean the deposition and accumulation of natural organic matter (NOM) composed by a variety of organic moieties on either a biotic or abiotic surface.
  • organic fouling layer should be understood to mean the deposition of colloids onto biotic or abiotic surfaces, where colloids should be understood to mean a homogeneous non-crystalline substance consisting of large molecules or ultramicroscopic particles dispersed through a second substance.
  • material surface should be understood to mean the outer layer or boundary of a material, such as, for example, a metal, a metal alloy, a polymer, a composite polymer, concrete and the like.
  • Figure 2 is a FTIR spectra of functionalised (Silica+PK) and non-functionalised
  • Si-NanoB Si-NanoB Spectra differences between functionalised and non-functionalised Si- NanoB are shown in red.
  • Figure 4 illustrates thermally prepared protein-based hydrogels drop-casted onto microscope glass slides,
  • Albumin-Peptone hydrogel This gel presents a very smooth and uniform surface: it is easy to mould (in this case it was drop-casted on a microscope glass), the jellification process is quick and the surface looks smooth and easy to analyse
  • (b) Albumin- Agar-Peptone hydrogel This formulation presented a very rough and brittle structure and it is very difficult to manipulate
  • (c) Albumin-Agar hydrogel The gel was prepared in different thermal conditions due to the presence of Agar. This gel is very brittle and difficult to handle.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Organic Chemistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Inorganic Chemistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

L'invention concerne une composition comprenant un nanoperle à base de silice dont la surface est fonctionnalisée par une fraction choisie parmi des fractions qui sont réactives à une couche d'encrassement sur une surface de matériau et se combinent avec celle-ci.
EP16778221.8A 2015-09-17 2016-09-16 Nanoperles fonctionnalisées par des enzymes destinées à être utilisées pour lutter contre l'encrassement biologique Withdrawn EP3350301A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP2015185598 2015-09-17
PCT/EP2016/071946 WO2017046313A1 (fr) 2015-09-17 2016-09-16 Nanoperles fonctionnalisées par des enzymes destinées à être utilisées pour lutter contre l'encrassement biologique

Publications (1)

Publication Number Publication Date
EP3350301A1 true EP3350301A1 (fr) 2018-07-25

Family

ID=62584860

Family Applications (1)

Application Number Title Priority Date Filing Date
EP16778221.8A Withdrawn EP3350301A1 (fr) 2015-09-17 2016-09-16 Nanoperles fonctionnalisées par des enzymes destinées à être utilisées pour lutter contre l'encrassement biologique

Country Status (1)

Country Link
EP (1) EP3350301A1 (fr)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10304331A1 (de) * 2003-02-04 2004-08-12 Henkel Kgaa Enzymatische Entfernung von Biofilmen auf Haushaltsoberflächen
WO2009085743A1 (fr) * 2007-12-20 2009-07-09 Danisco Us Inc., Genencor Division Prévention et suppression enzymatique de biofilms
WO2014067933A1 (fr) * 2012-10-31 2014-05-08 C-Lecta Gmbh Préparation de support bioactif pour une sécurité améliorée dans les produits de soin et les aliments
WO2014087011A1 (fr) * 2012-12-07 2014-06-12 Novozymes A/S Prévention de l'adhésion de bactéries
WO2017037602A1 (fr) * 2015-08-28 2017-03-09 Nasiertech S.R.L. Kit de bionettoyage et procédé d'élimination de films biologiques de substrats

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10304331A1 (de) * 2003-02-04 2004-08-12 Henkel Kgaa Enzymatische Entfernung von Biofilmen auf Haushaltsoberflächen
WO2009085743A1 (fr) * 2007-12-20 2009-07-09 Danisco Us Inc., Genencor Division Prévention et suppression enzymatique de biofilms
WO2014067933A1 (fr) * 2012-10-31 2014-05-08 C-Lecta Gmbh Préparation de support bioactif pour une sécurité améliorée dans les produits de soin et les aliments
WO2014087011A1 (fr) * 2012-12-07 2014-06-12 Novozymes A/S Prévention de l'adhésion de bactéries
WO2017037602A1 (fr) * 2015-08-28 2017-03-09 Nasiertech S.R.L. Kit de bionettoyage et procédé d'élimination de films biologiques de substrats

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of WO2017046313A1 *

Similar Documents

Publication Publication Date Title
Wang et al. Direct observation of microbial adhesion to membranes
Sigurdardóttir et al. Enzyme immobilization on inorganic surfaces for membrane reactor applications: mass transfer challenges, enzyme leakage and reuse of materials
Hwang et al. The role of conditioning film formation in Pseudomonas aeruginosa PAO1 adhesion to inert surfaces in aquatic environments
JP5723838B2 (ja) 大容量微粒子サンプル中の病原体検出
Behra et al. Magnetic porous sugar-functionalized PEG microgels for efficient isolation and removal of bacteria from solution
Hibbs et al. Designing a biocidal reverse osmosis membrane coating: Synthesis and biofouling properties
Debrassi et al. Stability of (bio) functionalized porous aluminum oxide
EP2588863B1 (fr) Capture de micro-organismes
Heyse et al. Protein immobilization using atmospheric‐pressure dielectric‐barrier discharges: a route to a straightforward manufacture of bioactive films
US11051517B2 (en) Enzyme-functionalised nanobeads for anti-biofouling purposes
Hansen et al. Lectin-functionalized poly (glycidyl methacrylate)-block-poly (vinyldimethyl azlactone) surface scaffolds for high avidity microbial capture
Chen et al. Preventing diatom adhesion using a hydrogel with an orthosilicic acid analog as a deceptive food
EP2087165A2 (fr) Matières biofonctionnelles
He et al. Alginate/albumin in incubation solution mediates the adhesion and biofilm formation of typical marine bacteria and algae
He et al. Participation of copper ions in formation of alginate conditioning layer: Evolved structure and regulated microbial adhesion
White et al. Influence of cell surface characteristics on adhesion of Saccharomyces cerevisiae to the biomaterial hydroxylapatite
Iguerb et al. Antifouling properties of poly (methyl methacrylate) films grafted with poly (ethylene glycol) monoacrylate immersed in seawater
WO2014156584A1 (fr) Capteur de détection de micro-organisme, son procédé de fabrication, et couche polymère
US8969058B2 (en) Method for releasing genetic material from solid phase
Bilad et al. A NOVEL IN-SITU ENZYMATIC CLEANING METHOD FOR REDUCING MEMBRANE FOULING IN MEMBRANE BIOREACTORS (MBRS
Chen et al. ATR-FTIR study of Bacillus sp. and Escherichia coli settlements on the bare and Al2O3 coated ZnSe internal reflection element
Pickering et al. Analysis of Giardia lamblia interactions with polymer surfaces using a microarray approach
WO2004104271A1 (fr) Procedes et compositions empechant les salissures par les proteines
Mahmoodi et al. Plasma Activation and its Nanoconfinement Effects Boost Surface Anti‐Biofouling Performance
Zhu et al. Antifouling performance of a membrane modified by phase-transited bovine serum albumin

Legal Events

Date Code Title Description
STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: UNKNOWN

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE

PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE

17P Request for examination filed

Effective date: 20180416

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

AX Request for extension of the european patent

Extension state: BA ME

RIN1 Information on inventor provided before grant (corrected)

Inventor name: AMADIO, JESSICA

Inventor name: HABIMANA, OLIVIER

Inventor name: ZANONI, MICHELLE

Inventor name: CASEY, EOIN

DAV Request for validation of the european patent (deleted)
DAX Request for extension of the european patent (deleted)
STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: EXAMINATION IS IN PROGRESS

17Q First examination report despatched

Effective date: 20190731

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20220423