EP3355939A4 - Mikrobielle deadman- und passcode-not-ausschalter - Google Patents

Mikrobielle deadman- und passcode-not-ausschalter Download PDF

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Publication number
EP3355939A4
EP3355939A4 EP16852709.1A EP16852709A EP3355939A4 EP 3355939 A4 EP3355939 A4 EP 3355939A4 EP 16852709 A EP16852709 A EP 16852709A EP 3355939 A4 EP3355939 A4 EP 3355939A4
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EP
European Patent Office
Prior art keywords
deadman
microbial
circuit breakers
passcode
type
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP16852709.1A
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English (en)
French (fr)
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EP3355939A2 (de
Inventor
Tsz Yan Clement Chan
James J. Collins
Jeong Wook Lee
Douglas Ewen Cameron
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Boston University
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Boston University
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Application filed by Boston University filed Critical Boston University
Publication of EP3355939A2 publication Critical patent/EP3355939A2/de
Publication of EP3355939A4 publication Critical patent/EP3355939A4/de
Withdrawn legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06N—COMPUTING ARRANGEMENTS BASED ON SPECIFIC COMPUTATIONAL MODELS
    • G06N3/00—Computing arrangements based on biological models
    • G06N3/12—Computing arrangements based on biological models using genetic models
    • G06N3/123—DNA computing

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  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Theoretical Computer Science (AREA)
  • Evolutionary Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Artificial Intelligence (AREA)
  • Computational Linguistics (AREA)
  • Data Mining & Analysis (AREA)
  • Evolutionary Computation (AREA)
  • Computing Systems (AREA)
  • General Physics & Mathematics (AREA)
  • Mathematical Physics (AREA)
  • Software Systems (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
EP16852709.1A 2015-09-30 2016-09-30 Mikrobielle deadman- und passcode-not-ausschalter Withdrawn EP3355939A4 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562235186P 2015-09-30 2015-09-30
PCT/US2016/054767 WO2017059245A2 (en) 2015-09-30 2016-09-30 Deadman and passcode microbial kill switches

Publications (2)

Publication Number Publication Date
EP3355939A2 EP3355939A2 (de) 2018-08-08
EP3355939A4 true EP3355939A4 (de) 2019-04-17

Family

ID=58427946

Family Applications (1)

Application Number Title Priority Date Filing Date
EP16852709.1A Withdrawn EP3355939A4 (de) 2015-09-30 2016-09-30 Mikrobielle deadman- und passcode-not-ausschalter

Country Status (3)

Country Link
US (1) US11459573B2 (de)
EP (1) EP3355939A4 (de)
WO (1) WO2017059245A2 (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2018379996A1 (en) 2017-12-05 2020-06-25 BioPlx, Inc. Methods and compositions to prevent microbial infection
US20220025364A1 (en) * 2020-06-02 2022-01-27 BioPlx, Inc. Methods for stable genomic integration in recombinant microorganisms
WO2026085495A1 (en) 2024-10-18 2026-04-23 Generation Bio Co. Synthetic partially single-stranded nucleic acid compositions and uses and methods therefor

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010075441A1 (en) * 2008-12-22 2010-07-01 Trustees Of Boston University Modular nucleic acid-based circuits for counters, binary operations, memory, and logic
WO2011066541A2 (en) * 2009-11-30 2011-06-03 Trustees Of Boston University Biological circuit chemotactic converters
WO2012170436A1 (en) * 2011-06-06 2012-12-13 The Regents Of The University Of California Synthetic biology tools
WO2014027351A1 (en) * 2012-08-16 2014-02-20 Yeda Research And Development Co. Ltd. At The Weizmann Institute Of Science A programmable nor-based device for transcription profile analyses
WO2014093852A1 (en) * 2012-12-13 2014-06-19 Massachusetts Institute Of Technology Recombinase-based logic and memory systems

Family Cites Families (73)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5928906A (en) 1996-05-09 1999-07-27 Sequenom, Inc. Process for direct sequencing during template amplification
US5972650A (en) 1997-06-26 1999-10-26 Brigham And Women's Hospital Tetracycline repressor regulated mammalian cell transcription and viral replication switch
DE10019058A1 (de) 2000-04-06 2001-12-20 Epigenomics Ag Detektion von Variationen des DNA-Methylierungsprofils
DE10056802B4 (de) 2000-11-14 2005-06-16 Epigenomics Ag Verfahren zur Detektion von Methylierungszuständen zur toxikologischen Diagnostik
US7642092B2 (en) 2003-03-03 2010-01-05 Technion Research & Development Foundation Ltd. Cultured cartilage/bone cells/tissue, method of generating same and uses thereof
EP1568786A3 (de) 2004-02-13 2007-08-29 Affymetrix, Inc. (A US Entity) Analyse der Methylierung mittels Nukleinsäurearrays
ES2787454T3 (es) 2004-09-30 2020-10-16 Epigenomics Ag Método para proveer fragmentos de ADN derivados de una muestra archivada
WO2006109300A1 (en) 2005-04-14 2006-10-19 Hadasit Medical Research Services & Development Ltd. Pre-transplantation treatment of donor cells to control graft versus host disease (gvhd) in transplant recipients
US7449297B2 (en) 2005-04-14 2008-11-11 Euclid Diagnostics Llc Methods of copying the methylation pattern of DNA during isothermal amplification and microarrays
WO2006113770A1 (en) 2005-04-15 2006-10-26 Epigenomics Ag A method for providing dna fragments derived from a remote sample
US20110172826A1 (en) 2005-12-14 2011-07-14 Amodei Dario G Device including altered microorganisms, and methods and systems of use
US8852916B2 (en) * 2010-01-22 2014-10-07 The Invention Science Fund I, Llc Compositions and methods for therapeutic delivery with microorganisms
CA2638750A1 (en) 2006-03-02 2007-09-13 Laboratory Corporation Of America Holdings Methods and systems for evaluating health risk factors by measurement of dna damage and dna repair
US9388425B2 (en) * 2006-10-20 2016-07-12 Trustees Of Boston University Tunable genetic switch for regulating gene expression
WO2008109797A1 (en) 2007-03-08 2008-09-12 Rules-Based Medicine, Inc. Methods for rapid disease screening
WO2008150853A1 (en) 2007-05-29 2008-12-11 Trustees Of Dartmouth College Compositions and methods for producing adaptive regulatory t cells
US8852864B2 (en) 2008-01-17 2014-10-07 Sequenom Inc. Methods and compositions for the analysis of nucleic acids
US9115386B2 (en) 2008-09-26 2015-08-25 Children's Medical Center Corporation Selective oxidation of 5-methylcytosine by TET-family proteins
US9175338B2 (en) 2008-12-11 2015-11-03 Pacific Biosciences Of California, Inc. Methods for identifying nucleic acid modifications
AU2009325069B2 (en) 2008-12-11 2015-03-19 Pacific Biosciences Of California, Inc. Classification of nucleic acid templates
JP2013514758A (ja) 2008-12-23 2013-05-02 ニユー・イングランド・バイオレイブス・インコーポレイテツド 修飾ｄｎａを切断するための組成物、方法および関連する使用
LT5708B (en) 2009-04-02 2011-01-25 Biotechnologijos Inst Derivatization of biomolecules by covalent coupling of non-cofactor compounds using methyltransferases
US8986928B2 (en) 2009-04-10 2015-03-24 Pacific Biosciences Of California, Inc. Nanopore sequencing devices and methods
EP2470675B1 (de) 2009-08-25 2016-03-30 New England Biolabs, Inc. Erkennung und quantifizierung hydroxymethylierter nukleotide in einem polynukleotidpräparat
US9697460B2 (en) * 2009-11-30 2017-07-04 Trustees Of Boston University Biological analog-to-digital and digital-to-analog converters
DE112011100297B4 (de) 2010-01-20 2017-08-24 New England Biolabs, Inc. Zusammensetzungen, Verfahren und damit in Zusammenhang stehende Anwendungen zum Spalten modifizierter DNA
US20120064521A1 (en) 2010-09-09 2012-03-15 James Yen Detection of dna hydroxymethylation
EP2630257B1 (de) 2010-10-22 2017-08-02 Oslo Universitetssykehus HF Verfahren und kits für den nachweis von 5-hydroxymethylcytosin
KR20130113476A (ko) 2010-10-27 2013-10-15 ?티백트 에이/에스 인터칼레이터 （ｉｎｔｅｒｃａｌａｔｏｒ） 분자를 포함하는 프로브에 의한 표적 ＤＮＡ 및 ＲＮＡ의 캡쳐
US20140178873A1 (en) 2011-03-04 2014-06-26 Andreas Brachmann Novel methods for detecting hydroxymethylcytosine
US9145580B2 (en) 2011-04-02 2015-09-29 New England Biolabs, Inc. Methods and compositions for enriching either target polynucleotides or non-target polynucleotides from a mixture of target and non-target polynucleotides
EP2694686B2 (de) 2011-04-06 2023-07-19 The University of Chicago Zusammensetzung und verfahren zur modifizierung von 5-methylcytosin (5mc)
US8951736B2 (en) 2011-04-29 2015-02-10 Sequenom, Inc. Multimer glycosylated nucleic acid binding protein conjugates and uses thereof
EP2723904A4 (de) 2011-06-27 2015-03-04 Flir Systems Verfahren und zusammensetzungen zum trennen einer zielnukleinsäure von gemischten nukleinsäureproben
GB201119903D0 (en) 2011-11-17 2011-12-28 Univ Vilnius Nucleic acid production and sequence analysis
CN103131754B (zh) 2011-11-24 2014-07-30 深圳华大基因科技服务有限公司 一种检测核酸羟甲基化修饰的方法及其应用
US20140179564A1 (en) 2012-11-01 2014-06-26 Pacific Biosciences Of California, Inc. Compositions and methods for selection of nucleic acids containing modified bases
US9238836B2 (en) 2012-03-30 2016-01-19 Pacific Biosciences Of California, Inc. Methods and compositions for sequencing modified nucleic acids
US9267117B2 (en) 2012-03-15 2016-02-23 New England Biolabs, Inc. Mapping cytosine modifications
US10081827B2 (en) 2012-03-15 2018-09-25 New England Biolabs, Inc. Mapping cytosine modifications
JP6224689B2 (ja) 2012-03-15 2017-11-01 ニユー・イングランド・バイオレイブス・インコーポレイテツド シトシンとこれの修飾物とを識別するための、およびメチローム分析のための方法および組成物
GB2559073A (en) 2012-06-08 2018-07-25 Pacific Biosciences California Inc Modified base detection with nanopore sequencing
WO2013188436A1 (en) 2012-06-11 2013-12-19 The Brigham And Women's Hospital, Inc. System and method for detecting cancer
GB201212047D0 (en) 2012-07-05 2012-08-22 Fermentas Uab Restriction endonucleases and their use
US9732390B2 (en) 2012-09-20 2017-08-15 The Chinese University Of Hong Kong Non-invasive determination of methylome of fetus or tumor from plasma
WO2014093924A1 (en) 2012-12-13 2014-06-19 Moderna Therapeutics, Inc. Modified nucleic acid molecules and uses thereof
CN103920965B (zh) 2013-01-16 2020-11-06 霍伯特兄弟公司 用于焊接电极的系统和方法
SG11201505618XA (en) 2013-01-22 2015-08-28 Centre Nat Rech Scient Process for detection of dna modifications and protein binding by single molecule manipulation
US20140272970A1 (en) 2013-03-15 2014-09-18 Promega Corporation Method for quantifying 5-hydroxymethylcytosine
KR20160011670A (ko) 2013-05-28 2016-02-01 라모트 앳 텔-아비브 유니버시티 리미티드 하이드록시메틸사이토신 염기의 검출
WO2014206568A1 (en) 2013-06-26 2014-12-31 Universität Konstanz Direct, programmable detection of epigenetic dna cytosine modifications using tal effectors
US10597647B2 (en) 2013-06-28 2020-03-24 New England Biolabs, Inc. Compositions and methods for identifying hydroxymethylcytosine in a DNA
US20160194696A1 (en) 2013-08-09 2016-07-07 New England Biolabs, Inc. Detecting, Sequencing and/or Mapping 5-Hydroxymethylcytosine and 5-Formylcytosine at Single-Base Resolution
EP3053585A1 (de) 2013-12-13 2016-08-10 Moderna Therapeutics, Inc. Alternative nukleinsäuremoleküle und verwendungen davon
GB201403216D0 (en) 2014-02-24 2014-04-09 Cambridge Epigenetix Ltd Nucleic acid sample preparation
EP3137633A4 (de) 2014-04-28 2017-11-29 Sigma-Aldrich Co. LLC Epigenetische modifizierung von säugergenomen mittels gezielter endonukleasen
US20170175129A1 (en) 2014-06-19 2017-06-22 Moderna Therapeutics, Inc. Alternative nucleic acid molecules and uses thereof
GB201415761D0 (en) 2014-09-05 2014-10-22 Cambridge Epigenetix Ltd Methods for detection of Nucleotide modification
EP3280793A4 (de) 2015-04-06 2018-10-03 The Regents of the University of California Verfahren zur bestimmung von basenstellen in einem polynukleotid
WO2017040477A1 (en) 2015-08-31 2017-03-09 The University Of Chicago Composition and methods for detecting adenosine modifications
WO2017039002A1 (ja) 2015-09-04 2017-03-09 国立大学法人東京大学 ５－ヒドロキシメチルシトシン酸化剤及び５－ヒドロキシメチルシトシン解析方法
AU2016323985B2 (en) 2015-09-17 2022-12-15 Novartis Ag CAR T cell therapies with enhanced efficacy
US10260088B2 (en) 2015-10-30 2019-04-16 New England Biolabs, Inc. Compositions and methods for analyzing modified nucleotides
LT3368688T (lt) 2015-10-30 2021-03-10 New England Biolabs, Inc. Kompozicijos ir būdai, skirti modifikuotų citozinų nustatymui sekvenavimu
EP3374524B1 (de) 2015-11-11 2025-01-29 Ramot at Tel-Aviv University Ltd. Verfahren zum nachweis von 5-hydroxymethylcytosin und zur diagnose von krebs
EP3214183B1 (de) 2016-03-01 2018-11-21 Universität Konstanz Decodierung auf basis von transkriptionsaktivatorartigem effektor (tale) von cytosinnukleobasen durch selektive modifikationsreaktion
US11162139B2 (en) 2016-03-02 2021-11-02 Shanghai Epican Genetech Co. Ltd. Method for genomic profiling of DNA 5-methylcytosine and 5-hydroxymethylcytosine
WO2017176630A1 (en) 2016-04-07 2017-10-12 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnostics by sequencing 5-hydroxymethylated cell-free dna
US20170298422A1 (en) 2016-04-18 2017-10-19 The Board Of Trustees Of The Leland Stanford Junior University Simultaneous single-molecule epigenetic imaging of dna methylation and hydroxymethylation
US20200190581A1 (en) 2017-01-04 2020-06-18 The University Of Chicago Methods for detecting cytosine modifications
CN117821565A (zh) 2017-03-08 2024-04-05 芝加哥大学 高灵敏度dna甲基化分析方法
US10155939B1 (en) 2017-06-15 2018-12-18 New England Biolabs, Inc. Method for performing multiple enzyme reactions in a single tube

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010075441A1 (en) * 2008-12-22 2010-07-01 Trustees Of Boston University Modular nucleic acid-based circuits for counters, binary operations, memory, and logic
WO2011066541A2 (en) * 2009-11-30 2011-06-03 Trustees Of Boston University Biological circuit chemotactic converters
WO2012170436A1 (en) * 2011-06-06 2012-12-13 The Regents Of The University Of California Synthetic biology tools
WO2014027351A1 (en) * 2012-08-16 2014-02-20 Yeda Research And Development Co. Ltd. At The Weizmann Institute Of Science A programmable nor-based device for transcription profile analyses
WO2014093852A1 (en) * 2012-12-13 2014-06-19 Massachusetts Institute Of Technology Recombinase-based logic and memory systems

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
BRYNNE C STANTON ET AL: "Genomic mining of prokaryotic repressors for orthogonal logic gates", NATURE CHEMICAL BIOLOGY, PUBLISHED ONLINE, vol. 10, no. 2, 8 December 2013 (2013-12-08), Basingstoke, pages 99 - 105, XP055563873, ISSN: 1552-4450, DOI: 10.1038/nchembio.1411 *
BRYNNE C STANTON ET AL: "SUPPLEMENTARY INFORMATION FOR: Genomic mining of prokaryotic repressors for orthogonal logic gates", NATURE CHEMICAL BIOLOGY, PUBLISHED ONLINE, vol. 10, no. 2, 8 December 2013 (2013-12-08), Basingstoke, pages 99 - 105, XP055563876, ISSN: 1552-4450, DOI: 10.1038/nchembio.1411 *
CLEMENT T Y CHAN ET AL: "'Deadman' and 'Passcode' microbial kill switches for bacterial containment", NATURE CHEMICAL BIOLOGY, PUBLISHED ONLINE, vol. 12, no. 2, 7 December 2015 (2015-12-07), Basingstoke, pages 82 - 86, XP055563186, ISSN: 1552-4450, DOI: 10.1038/nchembio.1979 *
CLEMENT T Y CHAN ET AL: "SUPPLEMENTARY INFORMATION FOR: 'Deadman' and 'Passcode' microbial kill switches for bacterial containment", NATURE CHEMICAL BIOLOGY PUBLISHED ONLINE, vol. 12, no. 2, 7 December 2015 (2015-12-07), Basingstoke, pages 82 - 86, XP055563381, ISSN: 1552-4450, DOI: 10.1038/nchembio.1979 *
HASTY JEFF ET AL: "Engineered gene circuits", NATURE, MACMILLAN JOURNALS LTD, LONDON, vol. 420, no. 6912, 14 November 2002 (2002-11-14), pages 224 - 230, XP002575083, ISSN: 0028-0836, DOI: 10.1038/NATURE01257 *
JENNIFER A N BROPHY ET AL: "Principles of genetic circuit design", NATURE METHODS, vol. 11, no. 5, 29 April 2014 (2014-04-29), New York, pages 508 - 520, XP055256504, ISSN: 1548-7091, DOI: 10.1038/nmeth.2926 *
LEE JEONG WOOK ET AL: "Creating Single-Copy Genetic Circuits.", MOLECULAR CELL 21 07 2016, vol. 63, no. 2, 21 July 2016 (2016-07-21), pages 329 - 336, XP002789320, ISSN: 1097-4164 *
See also references of WO2017059245A2 *

Also Published As

Publication number Publication date
WO2017059245A3 (en) 2017-08-03
EP3355939A2 (de) 2018-08-08
US20190040398A1 (en) 2019-02-07
US11459573B2 (en) 2022-10-04
WO2017059245A2 (en) 2017-04-06

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