EP3374505A4 - Recombinant host cells and methods for the anaerobic production of l-aspartate and beta-alanine - Google Patents

Recombinant host cells and methods for the anaerobic production of l-aspartate and beta-alanine Download PDF

Info

Publication number: EP3374505A4
Authority: EP; European Patent Office
Prior art keywords: aspartate; alanine; beta; methods; host cells
Prior art date: 2015-11-12
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Withdrawn

Application number

EP16865105.7A

Other languages

German (de)

French (fr)

Other versions

EP3374505A1 (en

Inventor

Jeffrey Dietrich

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Lygos Inc

Original Assignee

Lygos Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2015-11-12

Filing date

2016-11-11

Publication date

2019-05-01

2016-11-11 Application filed by Lygos Inc filed Critical Lygos Inc

2018-09-19 Publication of EP3374505A1 publication Critical patent/EP3374505A1/en

2019-05-01 Publication of EP3374505A4 publication Critical patent/EP3374505A4/en

Status Withdrawn legal-status Critical Current

Links

UCMIRNVEIXFBKS-UHFFFAOYSA-N beta-alanine Chemical compound NCCC(O)=O UCMIRNVEIXFBKS-UHFFFAOYSA-N 0.000 title 2
CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 title 1
229940000635 beta-alanine Drugs 0.000 title 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
- C12N9/0016—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with NAD or NADP as acceptor (1.4.1)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/06—Alanine; Leucine; Isoleucine; Serine; Homoserine
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/20—Aspartic acid; Asparagine
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y104/00—Oxidoreductases acting on the CH-NH2 group of donors (1.4)
- C12Y104/01—Oxidoreductases acting on the CH-NH2 group of donors (1.4) with NAD+ or NADP+ as acceptor (1.4.1)
- C12Y104/01021—Aspartate dehydrogenase (1.4.1.21)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y401/00—Carbon-carbon lyases (4.1)
- C12Y401/01—Carboxy-lyases (4.1.1)
- C12Y401/01011—Aspartate 1-decarboxylase (4.1.1.11)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y604/00—Ligases forming carbon-carbon bonds (6.4)
- C12Y604/01—Ligases forming carbon-carbon bonds (6.4.1)
- C12Y604/01001—Pyruvate carboxylase (6.4.1.1)

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Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Genetics & Genomics (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Biotechnology (AREA)
Microbiology (AREA)
Biomedical Technology (AREA)
Molecular Biology (AREA)
Physics & Mathematics (AREA)
Medicinal Chemistry (AREA)
Biophysics (AREA)
Chemical Kinetics & Catalysis (AREA)
General Chemical & Material Sciences (AREA)
Plant Pathology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Analytical Chemistry (AREA)
Immunology (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)

EP16865105.7A 2015-11-12 2016-11-11 Recombinant host cells and methods for the anaerobic production of l-aspartate and beta-alanine Withdrawn EP3374505A4 (en)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US201562254635P	2015-11-12	2015-11-12
PCT/US2016/061578 WO2017083683A1 (en)	2015-11-12	2016-11-11	Recombinant host cells and methods for the anaerobic production of l-aspartate and beta-alanine

Publications (2)

Publication Number	Publication Date
EP3374505A1 EP3374505A1 (en)	2018-09-19
EP3374505A4 true EP3374505A4 (en)	2019-05-01

Family

ID=58696123

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
EP16865105.7A Withdrawn EP3374505A4 (en)	2015-11-12	2016-11-11	Recombinant host cells and methods for the anaerobic production of l-aspartate and beta-alanine

Country Status (5)

Country	Link
EP (1)	EP3374505A4 (en)
CN (1)	CN109072172A (en)
AU (1)	AU2016353242A1 (en)
CA (1)	CA3042854A1 (en)
WO (1)	WO2017083683A1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
CN109593748B (en) *	2017-10-01	2022-03-04	宁波酶赛生物工程有限公司	Engineered decarboxylase polypeptide and application thereof in preparation of beta-alanine
US20220119821A1 (en) *	2018-06-25	2022-04-21	Lygos, Inc.	Recombinant host cells and methods for the production of aspartic acid and b-alanine
CN109735522B (en) *	2018-12-26	2021-02-02	浙江工业大学	A kind of L-aspartate-α-decarboxylase mutant and its application
CN111778200B (en) *	2019-04-04	2022-11-01	中国科学院微生物研究所	Platform bacterium for producing L-aspartic acid, recombinant bacterium for producing beta-alanine constructed based on platform bacterium and construction method thereof
US20250236896A1 (en)	2020-06-17	2025-07-24	Sumitomo Chemical Company, Limited	Transformant of genus hydrogenophilus bacterium capable of producing aspartic acid and methionine
CN112410245B (en) *	2020-10-13	2024-03-08	哈尔滨雁成生物科技有限公司	A low-temperature composting microbial compound inoculant and its preparation method and application
CN113604459B (en) *	2021-08-31	2023-10-10	江南大学	A kind of phosphoenolpyruvate carboxykinase and its application

Citations (3)

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WO2011087139A2 (en) *	2010-01-15	2011-07-21	Ajinomoto Co.,Inc.	A BACTERIUM OF Enterobacteriaceae FAMILY PRODUCING L-ASPARTIC ACID OR L-ASPARTIC ACID-DERIVED METABOLITES AND A METHOD FOR PRODUCING L-ASPARTIC ACID OR L-ASPARTIC ACID-DERIVED METABOLITES
WO2012074818A2 (en) *	2010-11-22	2012-06-07	Novozymes, Inc.	Compositions and methods for 3-hydroxypropionic acid production
WO2015031504A1 (en) *	2013-08-27	2015-03-05	The Regents Of The University Of California	RECOMBINANT PATHWAY AND ORGANISMS FOR MALONYL-CoA SYNTHESIS

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US8048624B1 (en) *	2007-12-04	2011-11-01	Opx Biotechnologies, Inc.	Compositions and methods for 3-hydroxypropionate bio-production from biomass
US20100021978A1 (en) *	2008-07-23	2010-01-28	Genomatica, Inc.	Methods and organisms for production of 3-hydroxypropionic acid
EP2483401B1 (en) *	2009-09-29	2017-06-21	Butamax (TM) Advanced Biofuels LLC	Improved yeast production host cells
KR20120083908A (en) *	2009-10-13	2012-07-26	게노마티카 인코포레이티드	Microorganisms for the production of 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-coa, putrescine and related compounds, and methods related thereto
BR112013003003A2 (en) *	2010-08-06	2016-06-14	Mascoma Corp	production of malonyl-coa derived products via anaerobic routes
CN114395496A (en) *	2012-03-06	2022-04-26	利戈斯股份有限公司	Recombinant host cells for the production of malonate

2016
- 2016-11-11 CN CN201680076240.7A patent/CN109072172A/en active Pending
- 2016-11-11 AU AU2016353242A patent/AU2016353242A1/en not_active Abandoned
- 2016-11-11 CA CA3042854A patent/CA3042854A1/en not_active Abandoned
- 2016-11-11 EP EP16865105.7A patent/EP3374505A4/en not_active Withdrawn
- 2016-11-11 WO PCT/US2016/061578 patent/WO2017083683A1/en not_active Ceased

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
WO2011087139A2 (en) *	2010-01-15	2011-07-21	Ajinomoto Co.,Inc.	A BACTERIUM OF Enterobacteriaceae FAMILY PRODUCING L-ASPARTIC ACID OR L-ASPARTIC ACID-DERIVED METABOLITES AND A METHOD FOR PRODUCING L-ASPARTIC ACID OR L-ASPARTIC ACID-DERIVED METABOLITES
WO2012074818A2 (en) *	2010-11-22	2012-06-07	Novozymes, Inc.	Compositions and methods for 3-hydroxypropionic acid production
WO2015031504A1 (en) *	2013-08-27	2015-03-05	The Regents Of The University Of California	RECOMBINANT PATHWAY AND ORGANISMS FOR MALONYL-CoA SYNTHESIS

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of WO2017083683A1 *

Also Published As

Publication number	Publication date
CN109072172A (en)	2018-12-21
AU2016353242A1 (en)	2018-06-21
WO2017083683A1 (en)	2017-05-18
CA3042854A1 (en)	2017-05-18
EP3374505A1 (en)	2018-09-19

Legal Events

Date	Code	Title	Description
2017-05-19	STAA	Information on the status of an ep patent application or granted ep patent	Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE
2018-08-17	PUAI	Public reference made under article 153(3) epc to a published international application that has entered the european phase	Free format text: ORIGINAL CODE: 0009012
2018-08-17	STAA	Information on the status of an ep patent application or granted ep patent	Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE
2018-09-19	17P	Request for examination filed	Effective date: 20180529
2018-09-19	AK	Designated contracting states	Kind code of ref document: A1 Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
2018-09-19	AX	Request for extension of the european patent	Extension state: BA ME
2018-09-26	RIN1	Information on inventor provided before grant (corrected)	Inventor name: DIETRICH, JEFFREY
2019-02-20	DAV	Request for validation of the european patent (deleted)
2019-02-20	DAX	Request for extension of the european patent (deleted)
2019-05-01	A4	Supplementary search report drawn up and despatched	Effective date: 20190403
2019-05-01	RIC1	Information provided on ipc code assigned before grant	Ipc: A61K 35/66 20150101ALI20190328BHEP Ipc: C12N 15/09 20060101AFI20190328BHEP Ipc: A61K 38/53 20060101ALI20190328BHEP Ipc: C12Q 1/68 20180101ALI20190328BHEP Ipc: C12N 15/66 20060101ALI20190328BHEP Ipc: A61K 38/44 20060101ALI20190328BHEP Ipc: A61K 38/51 20060101ALI20190328BHEP
2021-10-01	STAA	Information on the status of an ep patent application or granted ep patent	Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN
2021-11-03	18D	Application deemed to be withdrawn	Effective date: 20210601

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