EP3374505A4 - Rekombinante wirtszellen und verfahren zur anaeroben herstellung von l-aspartat und beta-alanin - Google Patents

Rekombinante wirtszellen und verfahren zur anaeroben herstellung von l-aspartat und beta-alanin Download PDF

Info

Publication number
EP3374505A4
EP3374505A4 EP16865105.7A EP16865105A EP3374505A4 EP 3374505 A4 EP3374505 A4 EP 3374505A4 EP 16865105 A EP16865105 A EP 16865105A EP 3374505 A4 EP3374505 A4 EP 3374505A4
Authority
EP
European Patent Office
Prior art keywords
aspartate
alanine
beta
methods
host cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP16865105.7A
Other languages
English (en)
French (fr)
Other versions
EP3374505A1 (de
Inventor
Jeffrey Dietrich
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lygos Inc
Original Assignee
Lygos Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lygos Inc filed Critical Lygos Inc
Publication of EP3374505A1 publication Critical patent/EP3374505A1/de
Publication of EP3374505A4 publication Critical patent/EP3374505A4/de
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0016—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with NAD or NADP as acceptor (1.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/06—Alanine; Leucine; Isoleucine; Serine; Homoserine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/20—Aspartic acid; Asparagine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y104/00—Oxidoreductases acting on the CH-NH2 group of donors (1.4)
    • C12Y104/01—Oxidoreductases acting on the CH-NH2 group of donors (1.4) with NAD+ or NADP+ as acceptor (1.4.1)
    • C12Y104/01021—Aspartate dehydrogenase (1.4.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01011—Aspartate 1-decarboxylase (4.1.1.11)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y604/00—Ligases forming carbon-carbon bonds (6.4)
    • C12Y604/01—Ligases forming carbon-carbon bonds (6.4.1)
    • C12Y604/01001—Pyruvate carboxylase (6.4.1.1)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
EP16865105.7A 2015-11-12 2016-11-11 Rekombinante wirtszellen und verfahren zur anaeroben herstellung von l-aspartat und beta-alanin Withdrawn EP3374505A4 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562254635P 2015-11-12 2015-11-12
PCT/US2016/061578 WO2017083683A1 (en) 2015-11-12 2016-11-11 Recombinant host cells and methods for the anaerobic production of l-aspartate and beta-alanine

Publications (2)

Publication Number Publication Date
EP3374505A1 EP3374505A1 (de) 2018-09-19
EP3374505A4 true EP3374505A4 (de) 2019-05-01

Family

ID=58696123

Family Applications (1)

Application Number Title Priority Date Filing Date
EP16865105.7A Withdrawn EP3374505A4 (de) 2015-11-12 2016-11-11 Rekombinante wirtszellen und verfahren zur anaeroben herstellung von l-aspartat und beta-alanin

Country Status (5)

Country Link
EP (1) EP3374505A4 (de)
CN (1) CN109072172A (de)
AU (1) AU2016353242A1 (de)
CA (1) CA3042854A1 (de)
WO (1) WO2017083683A1 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109593748B (zh) * 2017-10-01 2022-03-04 宁波酶赛生物工程有限公司 工程化脱羧酶多肽及其在制备β-丙氨酸中的应用
US20220119821A1 (en) * 2018-06-25 2022-04-21 Lygos, Inc. Recombinant host cells and methods for the production of aspartic acid and b-alanine
CN109735522B (zh) * 2018-12-26 2021-02-02 浙江工业大学 一种L-天冬氨酸-α-脱羧酶突变体及其应用
CN111778200B (zh) * 2019-04-04 2022-11-01 中国科学院微生物研究所 生产L-天冬氨酸的平台菌、基于该平台菌构建的生产β-丙氨酸的重组菌及其构建方法
US20250236896A1 (en) 2020-06-17 2025-07-24 Sumitomo Chemical Company, Limited Transformant of genus hydrogenophilus bacterium capable of producing aspartic acid and methionine
CN112410245B (zh) * 2020-10-13 2024-03-08 哈尔滨雁成生物科技有限公司 一种低温堆肥微生物复合菌剂及其制备方法和应用
CN113604459B (zh) * 2021-08-31 2023-10-10 江南大学 一种磷酸烯醇式丙酮酸羧激酶及其应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011087139A2 (en) * 2010-01-15 2011-07-21 Ajinomoto Co.,Inc. A BACTERIUM OF Enterobacteriaceae FAMILY PRODUCING L-ASPARTIC ACID OR L-ASPARTIC ACID-DERIVED METABOLITES AND A METHOD FOR PRODUCING L-ASPARTIC ACID OR L-ASPARTIC ACID-DERIVED METABOLITES
WO2012074818A2 (en) * 2010-11-22 2012-06-07 Novozymes, Inc. Compositions and methods for 3-hydroxypropionic acid production
WO2015031504A1 (en) * 2013-08-27 2015-03-05 The Regents Of The University Of California RECOMBINANT PATHWAY AND ORGANISMS FOR MALONYL-CoA SYNTHESIS

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8048624B1 (en) * 2007-12-04 2011-11-01 Opx Biotechnologies, Inc. Compositions and methods for 3-hydroxypropionate bio-production from biomass
US20100021978A1 (en) * 2008-07-23 2010-01-28 Genomatica, Inc. Methods and organisms for production of 3-hydroxypropionic acid
EP2483401B1 (de) * 2009-09-29 2017-06-21 Butamax (TM) Advanced Biofuels LLC Wirtszellen mit erhöhter hefeproduktion
KR20120083908A (ko) * 2009-10-13 2012-07-26 게노마티카 인코포레이티드 1,4-부탄다이올, 4-하이드록시부탄알, 4-하이드록시부티릴-ｃｏａ, 푸트레신 및 관련 화합물의 제조를 위한 미생물 및 관련 방법
BR112013003003A2 (pt) * 2010-08-06 2016-06-14 Mascoma Corp produção de produtos malonil-coa derivados através de rotas anaeróbicas
CN114395496A (zh) * 2012-03-06 2022-04-26 利戈斯股份有限公司 用于产生丙二酸的重组宿主细胞

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011087139A2 (en) * 2010-01-15 2011-07-21 Ajinomoto Co.,Inc. A BACTERIUM OF Enterobacteriaceae FAMILY PRODUCING L-ASPARTIC ACID OR L-ASPARTIC ACID-DERIVED METABOLITES AND A METHOD FOR PRODUCING L-ASPARTIC ACID OR L-ASPARTIC ACID-DERIVED METABOLITES
WO2012074818A2 (en) * 2010-11-22 2012-06-07 Novozymes, Inc. Compositions and methods for 3-hydroxypropionic acid production
WO2015031504A1 (en) * 2013-08-27 2015-03-05 The Regents Of The University Of California RECOMBINANT PATHWAY AND ORGANISMS FOR MALONYL-CoA SYNTHESIS

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of WO2017083683A1 *

Also Published As

Publication number Publication date
CN109072172A (zh) 2018-12-21
AU2016353242A1 (en) 2018-06-21
WO2017083683A1 (en) 2017-05-18
CA3042854A1 (en) 2017-05-18
EP3374505A1 (de) 2018-09-19

Similar Documents

Publication Publication Date Title
EP3273976A4 (de) Modifizierte t-zellen und verfahren zur herstellung und verwendung davon
EP3328399A4 (de) Modifizierte zellen und therapieverfahren
EP3322711A4 (de) Hpk1-inhibitoren und verfahren zur verwendung davon
EP3231024A4 (de) Elektrochemische zelle und verfahren zur herstellung davon
EP3302541A4 (de) Verfahren und vorrichtungen zur herstellung und ausgabe von vorteilhaften faktoren von stammzellen
EP3143134A4 (de) Modifizierte natürliche killerzellen und verwendungen davon
EP3212225A4 (de) Verfahren und zusammensetzungen für modifizierte t-zellen
EP3240552A4 (de) Natürliche killerzellen und verwendungen davon
EP3124589A4 (de) Zellkultursystem und zellkulturverfahren
EP3150704A4 (de) Verfahren zur kultivierung und zellmasse
EP3094718A4 (de) Mikroorganismen für die erhöhte produktion von aminosäuren und zugehörige verfahren
EP3374505A4 (de) Rekombinante wirtszellen und verfahren zur anaeroben herstellung von l-aspartat und beta-alanin
EP3245303A4 (de) Altersmodifizierte zellen und verfahren zur herstellung altersmodifizierter zellen
EP3365107B8 (de) Zellkultur
EP3252141A4 (de) Zellkulturverfahren und kit
EP3325608A4 (de) Verfahren und mikroorganismen zur herstellung von 1,3-butandiol
EP3167491A4 (de) Photovoltaische verbundhalbleiter-taic-zelle und herstellungsverfahren einer photovoltaischen verbundhalbleiter-taic-zelle
EP3149212A4 (de) Elektrochemische verfahren und verbindungen zur detektion von enzymen
EP3144315A4 (de) Enzymspezifische fluoreszierende verbindung haltbare in zellen
EP3255147A4 (de) Immobilisierte zelle und herstellungsverfahren dafür
EP3140392A4 (de) Herstellung von heteromultimeren proteinen mittels säugerzellen
EP3096892A4 (de) Kohlenstoffnanoröhrchenbeschichtete substrate und verfahren zur herstellung davon
EP3288570A4 (de) Modifizierte stammzellen und verwendungen davon
EP3158077A4 (de) Zellkulturverfahren und medium mit n-acetylcystein
EP3166626A4 (de) Materialien und verfahren zur behandlung von leiden in zusammenhang mit sulfataseenzymen

Legal Events

Date Code Title Description
STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE

PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE

17P Request for examination filed

Effective date: 20180529

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

AX Request for extension of the european patent

Extension state: BA ME

RIN1 Information on inventor provided before grant (corrected)

Inventor name: DIETRICH, JEFFREY

DAV Request for validation of the european patent (deleted)
DAX Request for extension of the european patent (deleted)
A4 Supplementary search report drawn up and despatched

Effective date: 20190403

RIC1 Information provided on ipc code assigned before grant

Ipc: A61K 35/66 20150101ALI20190328BHEP

Ipc: C12N 15/09 20060101AFI20190328BHEP

Ipc: A61K 38/53 20060101ALI20190328BHEP

Ipc: C12Q 1/68 20180101ALI20190328BHEP

Ipc: C12N 15/66 20060101ALI20190328BHEP

Ipc: A61K 38/44 20060101ALI20190328BHEP

Ipc: A61K 38/51 20060101ALI20190328BHEP

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20210601