EP3600406A1 - Vaccins bivalents à base de vlp contre le virus ebola et leurs procédés de fabrication et d'utilisation - Google Patents
Vaccins bivalents à base de vlp contre le virus ebola et leurs procédés de fabrication et d'utilisationInfo
- Publication number
- EP3600406A1 EP3600406A1 EP18778205.7A EP18778205A EP3600406A1 EP 3600406 A1 EP3600406 A1 EP 3600406A1 EP 18778205 A EP18778205 A EP 18778205A EP 3600406 A1 EP3600406 A1 EP 3600406A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- ebola
- composition
- vlp
- cell line
- gag
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
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- A61P37/00—Drugs for immunological or allergic disorders
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- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
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- C—CHEMISTRY; METALLURGY
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- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
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- C12N2760/14134—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
Definitions
- FIG. 1 Inducible expression of EBOV GP, SUDV GP and HIV-1 Gag in ESGPGag 293F cells. Cells were cultured as such or induced with Dox for 24 h and cell ly sates probed by western blotting for EBOV GP, SUDV GP, HIV-1 Gag and actin using specific antibodies.
- the disclosed VLP platform may be used for large-scale production of a nanoparticle bivalent Ebola vaccine.
- the production system is based on a stable and inducible ESGPGag 293F cell line that, upon induction with doxycycline (Dox), secretes VLPs with HIV-1 Gag core abundantly studded with Ebola glycoproteins (GPs) from Zaire (EBOV) and Sudan (SUDV) Ebolaviruses.
- the Gag core provides a very stable framework of ⁇ 1 lOnm on which Ebola GPs are incorporated in their native conformation.
- Initial immunogenicity results in rabbits are highly promising, with high binding titers and substantial neutralization elicited against all the four pathogenic Ebolavirus species.
- Sequence identity may be measured using sequence analysis software on the default setting (e.g., Sequence Analysis Software Package of the Genetics Computer Group, University of Wisconsin Biotechnology Center, 1710 University Avenue, Madison, Wis. 53705). Such software may match similar sequences by assigning degrees of homology to various substitutions, deletions, and other modifications.
- compositions herein may incorporate known injectable, physiologically acceptable sterile solutions.
- aqueous isotonic solutions such as e.g. saline or corresponding plasma protein solutions are readily available.
- the immunogenic and vaccine compositions of the present invention can include diluents, isotonic agents, stabilizers, or adjuvants.
- Diluents can include water, saline, dextrose, ethanol, glycerol, and the like.
- Isotonic agents can include sodium chloride, dextrose, mannitol, sorbitol, and lactose, among others.
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Abstract
L'invention concerne des compositions de vaccin bivalent à base de pseudo-particules virales (VLP). Les compositions peuvent comprendre un noyau protéique de l'antigène « Gag » sphérique et au moins deux glycoprotéines du virus Ebola. Lesdites au moins deux glycoprotéines du virus Ebola peuvent être situées au niveau de la surface extérieure du noyau protéique Gag sphérique, de sorte que le vaccin à base de VLP présente au moins deux antigènes de glycoprotéine du virus Ebola. Dans un aspect, lesdites au moins deux glycoprotéines du virus Ebola sont une glycoprotéine du virus Ebola Zaïre (EBOV) et une glycoprotéine du virus Ebola Soudan (SUDV).
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762477480P | 2017-03-28 | 2017-03-28 | |
| PCT/US2018/024747 WO2018183435A1 (fr) | 2017-03-28 | 2018-03-28 | Vaccins bivalents à base de vlp contre le virus ebola et leurs procédés de fabrication et d'utilisation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| EP3600406A1 true EP3600406A1 (fr) | 2020-02-05 |
| EP3600406A4 EP3600406A4 (fr) | 2021-01-13 |
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| Application Number | Title | Priority Date | Filing Date |
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| EP18778205.7A Pending EP3600406A4 (fr) | 2017-03-28 | 2018-03-28 | Vaccins bivalents à base de vlp contre le virus ebola et leurs procédés de fabrication et d'utilisation |
| EP18776312.3A Pending EP3600367A4 (fr) | 2017-03-28 | 2018-03-28 | Vaccins monovalents à base de vlp contre ebola et leurs procédés de préparation et d'utilisation |
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| EP18776312.3A Pending EP3600367A4 (fr) | 2017-03-28 | 2018-03-28 | Vaccins monovalents à base de vlp contre ebola et leurs procédés de préparation et d'utilisation |
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| US (4) | US11129886B2 (fr) |
| EP (2) | EP3600406A4 (fr) |
| CA (2) | CA3056927A1 (fr) |
| WO (2) | WO2018183443A1 (fr) |
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| US11129886B2 (en) | 2017-03-28 | 2021-09-28 | Children's Hospital Medical Center | VLP-based bivalent Ebola vaccines and methods of making and using same |
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| US9045727B2 (en) | 2002-05-17 | 2015-06-02 | Emory University | Virus-like particles, methods of preparation, and immunogenic compositions |
| JP2009519715A (ja) * | 2005-12-16 | 2009-05-21 | ザ ユーエービー リサーチ ファウンデーション | ウイルスベクターを使用する制御された遺伝子発現に関する組成物および方法 |
| US20100143406A1 (en) | 2006-06-30 | 2010-06-10 | Gale Smith | Methods of enhancing protein incorporation into virus like particles |
| WO2012122858A1 (fr) * | 2011-03-17 | 2012-09-20 | 中国科学院上海巴斯德研究所 | Procédé de production d'une particule apparentée à un virus par utilisation de cellule de drosophila et ses applications |
| CA3194125A1 (fr) * | 2012-04-12 | 2013-10-17 | The Trustees Of The University Of Pennsylvania | Antigenes consensus de filovirus, constructions d'acides nucleiques et vaccins a base de ceux-ci, et leurs methodes d'utilisation |
| WO2015066715A1 (fr) | 2013-11-04 | 2015-05-07 | Viracell Advanced Products, Llc | Pseudo-particules virales et procédés qui leur sont associés |
| US9969986B2 (en) | 2014-08-08 | 2018-05-15 | Vlp Therapeutics, Llc | Virus like particle comprising modified envelope protein E3 |
| WO2016168187A1 (fr) | 2015-04-13 | 2016-10-20 | The Regents Of The University Of Michigan | Particules de type virus |
| WO2017015457A1 (fr) * | 2015-07-21 | 2017-01-26 | Modernatx, Inc. | Vaccin contre le virus ebola |
| US11129886B2 (en) | 2017-03-28 | 2021-09-28 | Children's Hospital Medical Center | VLP-based bivalent Ebola vaccines and methods of making and using same |
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2018
- 2018-03-28 US US16/494,841 patent/US11129886B2/en active Active
- 2018-03-28 EP EP18778205.7A patent/EP3600406A4/fr active Pending
- 2018-03-28 WO PCT/US2018/024758 patent/WO2018183443A1/fr not_active Ceased
- 2018-03-28 WO PCT/US2018/024747 patent/WO2018183435A1/fr not_active Ceased
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- 2018-03-28 US US16/494,843 patent/US12427190B2/en active Active
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Also Published As
| Publication number | Publication date |
|---|---|
| EP3600406A4 (fr) | 2021-01-13 |
| WO2018183435A1 (fr) | 2018-10-04 |
| EP3600367A4 (fr) | 2021-01-13 |
| WO2018183443A1 (fr) | 2018-10-04 |
| US20200085936A1 (en) | 2020-03-19 |
| US12280100B2 (en) | 2025-04-22 |
| EP3600367A1 (fr) | 2020-02-05 |
| US11890337B2 (en) | 2024-02-06 |
| US11129886B2 (en) | 2021-09-28 |
| US20200085937A1 (en) | 2020-03-19 |
| CA3056927A1 (fr) | 2018-10-04 |
| CA3057138A1 (fr) | 2018-10-04 |
| US20240131141A1 (en) | 2024-04-25 |
| US20210386849A1 (en) | 2021-12-16 |
| US12427190B2 (en) | 2025-09-30 |
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