EP3638809A4 - Negativ-positive anreicherung für den nachweis von nukleinsäuren - Google Patents

Negativ-positive anreicherung für den nachweis von nukleinsäuren Download PDF

Info

Publication number: EP3638809A4
Authority: EP; European Patent Office
Prior art keywords: negative; nucleic acid; acid detection; positive enrichment; enrichment
Prior art date: 2017-06-13
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Withdrawn

Application number

EP18817717.4A

Other languages

English (en)

French (fr)

Other versions

EP3638809A1 (de

Inventor

Anthony P. Shuber

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Genetics Research LLC

Original Assignee

Genetics Research LLC

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2017-06-13

Filing date

2018-06-13

Publication date

2021-03-10

2018-01-23 Priority claimed from US15/877,619 external-priority patent/US10527608B2/en

2018-06-13 Application filed by Genetics Research LLC filed Critical Genetics Research LLC

2020-04-22 Publication of EP3638809A1 publication Critical patent/EP3638809A1/de

2021-03-10 Publication of EP3638809A4 publication Critical patent/EP3638809A4/de

Status Withdrawn legal-status Critical Current

Links

238000001514 detection method Methods 0.000 title 1
150000007523 nucleic acids Chemical class 0.000 title 1
102000039446 nucleic acids Human genes 0.000 title 1
108020004707 nucleic acids Proteins 0.000 title 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

Landscapes

Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Microbiology (AREA)
Biochemistry (AREA)
Physics & Mathematics (AREA)
Molecular Biology (AREA)
Biotechnology (AREA)
Biophysics (AREA)
Analytical Chemistry (AREA)
Immunology (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Saccharide Compounds (AREA)

EP18817717.4A 2017-06-13 2018-06-13 Negativ-positive anreicherung für den nachweis von nukleinsäuren Withdrawn EP3638809A4 (de)

Applications Claiming Priority (6)

Application Number	Priority Date	Filing Date	Title
US201762519051P	2017-06-13	2017-06-13
US201762526091P	2017-06-28	2017-06-28
US201762568121P	2017-10-04	2017-10-04
US15/877,619 US10527608B2 (en)	2017-06-13	2018-01-23	Methods for rare event detection
US201862656592P	2018-04-12	2018-04-12
PCT/US2018/037277 WO2018231945A1 (en)	2017-06-13	2018-06-13	Negative-positive enrichment for nucleic acid detection

Publications (2)

Publication Number	Publication Date
EP3638809A1 EP3638809A1 (de)	2020-04-22
EP3638809A4 true EP3638809A4 (de)	2021-03-10

Family

ID=64659281

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
EP18817717.4A Withdrawn EP3638809A4 (de)	2017-06-13	2018-06-13	Negativ-positive anreicherung für den nachweis von nukleinsäuren

Country Status (3)

Country	Link
EP (1)	EP3638809A4 (de)
CA (2)	CA3069934A1 (de)
WO (1)	WO2018231945A1 (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US10081829B1 (en)	2017-06-13	2018-09-25	Genetics Research, Llc	Detection of targeted sequence regions
EP3625356B1 (de)	2017-08-08	2021-05-19	Depixus	In-vitro-isolierung und anreicherung von nukleinsäuren mittels ortsspezifischer nuklease
US20210040537A1 (en) *	2019-08-08	2021-02-11	Genetics Research, Llc	Selective enrichment
AU2023364547A1 (en)	2022-10-21	2025-04-10	Watchmaker Genomics, Inc.	Methods and compositions for sequencing library normalization

Citations (5)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
WO2016028887A1 (en) *	2014-08-19	2016-02-25	Pacific Biosciences Of California, Inc.	Compositions and methods for enrichment of nucleic acids
WO2017031360A1 (en) *	2015-08-19	2017-02-23	Arc Bio, Llc	Capture of nucleic acids using a nucleic acid-guided nuclease-based system
EP3150718A1 (de) *	2014-05-28	2017-04-05	Toolgen Incorporated	Verfahren zur empfindlichen detektion von ziel-dna mit zielspezifischer nuklease
WO2019030306A1 (en) *	2017-08-08	2019-02-14	Depixus	ISOLATION AND IN VITRO ENRICHMENT OF NUCLEIC ACIDS USING SITE-SPECIFIC NUCLEASES
WO2019178577A1 (en) *	2018-03-15	2019-09-19	Twinstrand Biosciences, Inc.	Methods and reagents for enrichment of nucleic acid material for sequencing applications and other nucleic acid material interrogations

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
CA2817990A1 (en) *	2009-12-23	2011-06-30	Genetic Technologies Limited	Methods of enriching and detecting fetal nucleic acids
US10421993B2 (en) *	2015-02-11	2019-09-24	Paragon Genomics, Inc.	Methods and compositions for reducing non-specific amplification products
WO2016134136A2 (en) *	2015-02-20	2016-08-25	The Johns Hopkins University	Genomic alterations in the tumor and circulation of pancreatic cancer patients
US11268144B2 (en) *	2015-09-24	2022-03-08	Sigma-Aldrich Co. Llc	Methods and reagents for molecular proximity detection using RNA-guided nucleic acid binding proteins

2018
- 2018-06-13 CA CA3069934A patent/CA3069934A1/en not_active Abandoned
- 2018-06-13 EP EP18817717.4A patent/EP3638809A4/de not_active Withdrawn
- 2018-06-13 CA CA3222142A patent/CA3222142A1/en active Pending
- 2018-06-13 WO PCT/US2018/037277 patent/WO2018231945A1/en not_active Ceased

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
EP3150718A1 (de) *	2014-05-28	2017-04-05	Toolgen Incorporated	Verfahren zur empfindlichen detektion von ziel-dna mit zielspezifischer nuklease
WO2016028887A1 (en) *	2014-08-19	2016-02-25	Pacific Biosciences Of California, Inc.	Compositions and methods for enrichment of nucleic acids
WO2017031360A1 (en) *	2015-08-19	2017-02-23	Arc Bio, Llc	Capture of nucleic acids using a nucleic acid-guided nuclease-based system
WO2019030306A1 (en) *	2017-08-08	2019-02-14	Depixus	ISOLATION AND IN VITRO ENRICHMENT OF NUCLEIC ACIDS USING SITE-SPECIFIC NUCLEASES
WO2019178577A1 (en) *	2018-03-15	2019-09-19	Twinstrand Biosciences, Inc.	Methods and reagents for enrichment of nucleic acid material for sequencing applications and other nucleic acid material interrogations

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
JENNIFER L. STEELE ET AL: "Novel CRISPR-based sequence specific enrichment methods for target loci and single base mutations", PLOS ONE, vol. 15, no. 12, 23 December 2020 (2020-12-23), pages e0243781, XP055768725, DOI: 10.1371/journal.pone.0243781 *
RICHARD C. STEVENS ET AL: "A novel CRISPR/Cas9 associated technology for sequence-specific nucleic acid enrichment", PLOS ONE, vol. 14, no. 4, 18 April 2019 (2019-04-18), pages e0215441, XP055751103, DOI: 10.1371/journal.pone.0215441 *
See also references of WO2018231945A1 *

Also Published As

Publication number	Publication date
EP3638809A1 (de)	2020-04-22
CA3222142A1 (en)	2018-12-20
WO2018231945A1 (en)	2018-12-20
CA3069934A1 (en)	2018-12-20

Legal Events

Date	Code	Title	Description
2018-12-22	STAA	Information on the status of an ep patent application or granted ep patent	Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE
2020-03-20	PUAI	Public reference made under article 153(3) epc to a published international application that has entered the european phase	Free format text: ORIGINAL CODE: 0009012
2020-03-20	STAA	Information on the status of an ep patent application or granted ep patent	Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE
2020-04-22	17P	Request for examination filed	Effective date: 20200113
2020-04-22	AK	Designated contracting states	Kind code of ref document: A1 Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
2020-04-22	AX	Request for extension of the european patent	Extension state: BA ME
2020-09-16	DAV	Request for validation of the european patent (deleted)
2020-09-16	DAX	Request for extension of the european patent (deleted)
2021-03-10	A4	Supplementary search report drawn up and despatched	Effective date: 20210208
2021-03-10	RIC1	Information provided on ipc code assigned before grant	Ipc: C12Q 1/6848 20180101ALI20210202BHEP Ipc: C12Q 1/686 20180101ALI20210202BHEP Ipc: C12Q 1/6853 20180101ALI20210202BHEP Ipc: C12Q 1/68 20180101AFI20210202BHEP Ipc: C12Q 1/6806 20180101ALI20210202BHEP
2023-04-28	STAA	Information on the status of an ep patent application or granted ep patent	Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN
2023-05-31	18D	Application deemed to be withdrawn	Effective date: 20230103

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