EP3645728A4 - Nouveaux orthologues de crispr de type vi et systèmes associés - Google Patents

Nouveaux orthologues de crispr de type vi et systèmes associés Download PDF

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Publication number
EP3645728A4
EP3645728A4 EP18824952.8A EP18824952A EP3645728A4 EP 3645728 A4 EP3645728 A4 EP 3645728A4 EP 18824952 A EP18824952 A EP 18824952A EP 3645728 A4 EP3645728 A4 EP 3645728A4
Authority
EP
European Patent Office
Prior art keywords
orthologists
related systems
crispr type
new crispr
new
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP18824952.8A
Other languages
German (de)
English (en)
Other versions
EP3645728A1 (fr
Inventor
Feng Zhang
Omar O. Abudayyeh
David Benjamin Turitz Cox
Jonathan S. Gootenberg
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Massachusetts Institute of Technology
Broad Institute Inc
Harvard University
Original Assignee
Massachusetts Institute of Technology
Broad Institute Inc
Harvard University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Massachusetts Institute of Technology, Broad Institute Inc, Harvard University filed Critical Massachusetts Institute of Technology
Publication of EP3645728A1 publication Critical patent/EP3645728A1/fr
Publication of EP3645728A4 publication Critical patent/EP3645728A4/fr
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/71—Fusion polypeptide containing domain for protein-protein interaction containing domain for transcriptional activaation, e.g. VP16
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/85—Fusion polypeptide containing an RNA binding domain
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/90—Fusion polypeptide containing a motif for post-translational modification

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
EP18824952.8A 2017-06-26 2018-06-26 Nouveaux orthologues de crispr de type vi et systèmes associés Pending EP3645728A4 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762525165P 2017-06-26 2017-06-26
US201762566851P 2017-10-02 2017-10-02
PCT/US2018/039595 WO2019005866A1 (fr) 2017-06-26 2018-06-26 Nouveaux orthologues de crispr de type vi et systèmes associés

Publications (2)

Publication Number Publication Date
EP3645728A1 EP3645728A1 (fr) 2020-05-06
EP3645728A4 true EP3645728A4 (fr) 2021-03-24

Family

ID=64741890

Family Applications (1)

Application Number Title Priority Date Filing Date
EP18824952.8A Pending EP3645728A4 (fr) 2017-06-26 2018-06-26 Nouveaux orthologues de crispr de type vi et systèmes associés

Country Status (2)

Country Link
EP (1) EP3645728A4 (fr)
WO (1) WO2019005866A1 (fr)

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WO2015105955A1 (fr) 2014-01-08 2015-07-16 Flodesign Sonics, Inc. Dispositif d'acoustophorèse avec double chambre acoustophorétique
US11708572B2 (en) 2015-04-29 2023-07-25 Flodesign Sonics, Inc. Acoustic cell separation techniques and processes
US12404514B2 (en) 2016-12-09 2025-09-02 The Broad Institute, Inc. CRISPR-systems for modifying a trait of interest in a plant
US11174515B2 (en) 2017-03-15 2021-11-16 The Broad Institute, Inc. CRISPR effector system based diagnostics
US11104937B2 (en) 2017-03-15 2021-08-31 The Broad Institute, Inc. CRISPR effector system based diagnostics
WO2018183908A1 (fr) 2017-03-31 2018-10-04 Dana-Farber Cancer Institute, Inc. Compositions et méthodes de traitement de tumeurs ovariennes
WO2018191558A1 (fr) 2017-04-12 2018-10-18 The Broad Institute, Inc. Modulation de la différenciation, de la maintenance et/ou de la fonction de cellules épithéliales par l'action de lymphocytes t, et marqueurs et méthodes d'utilisation associées
US11618928B2 (en) 2017-04-12 2023-04-04 The Broad Institute, Inc. CRISPR effector system based diagnostics for malaria detection
WO2019010422A1 (fr) 2017-07-07 2019-01-10 The Broad Institute, Inc. Thérapie antivirale basée sur le système crispr
US12049643B2 (en) 2017-07-14 2024-07-30 The Broad Institute, Inc. Methods and compositions for modulating cytotoxic lymphocyte activity
EP3654993A4 (fr) 2017-07-17 2021-08-25 The Broad Institute, Inc. Atlas de cellules du côlon humain en bonne santé et avec recto-colite hémorragique
EP3692146A4 (fr) 2017-10-04 2021-06-30 The Broad Institute, Inc. Diagnostics basés sur un système effecteur crispr
WO2019094955A1 (fr) 2017-11-13 2019-05-16 The Broad Institute, Inc. Méthodes et compositions pour cibler des programmes oncogènes et de développement dans les gliomes h3k27m
WO2019113506A1 (fr) 2017-12-07 2019-06-13 The Broad Institute, Inc. Procédés et compositions pour multiplexer un séquençage de noyaux isolés et de cellules isolées
WO2019118921A1 (fr) 2017-12-14 2019-06-20 Flodesign Sonics, Inc. Circuit d'excitation et circuit de commande de transducteur acoustique
US11957695B2 (en) 2018-04-26 2024-04-16 The Broad Institute, Inc. Methods and compositions targeting glucocorticoid signaling for modulating immune responses
US12036240B2 (en) 2018-06-14 2024-07-16 The Broad Institute, Inc. Compositions and methods targeting complement component 3 for inhibiting tumor growth
MX2020013461A (es) 2018-06-26 2021-04-28 Broad Inst Inc Composiciones, sistemas y métodos de amplificación basados en la doble nicasa crispr.
CA3102163A1 (fr) 2018-06-26 2020-01-02 Massachusetts Institute Of Technology Procedes et systemes d'amplification fondes sur un systeme effecteur crispr et diagnostics associes
WO2020041380A1 (fr) 2018-08-20 2020-02-27 The Broad Institute, Inc. Méthodes et compositions de régulation optochimique de crispr-cas9
US12402610B2 (en) 2018-11-09 2025-09-02 The Broad Institute, Inc. Methods and compositions for modulating innate lymphoid cell pathogenic effectors
WO2020124050A1 (fr) 2018-12-13 2020-06-18 The Broad Institute, Inc. Dosages en mosaïque à l'aide d'une détection basée sur crispr-cas
US11739156B2 (en) 2019-01-06 2023-08-29 The Broad Institute, Inc. Massachusetts Institute of Technology Methods and compositions for overcoming immunosuppression
CN110157695A (zh) * 2019-01-25 2019-08-23 南昌大学 一种植物RNA m5C甲基化修饰的系统及方法
WO2020186231A2 (fr) 2019-03-14 2020-09-17 The Broad Institute, Inc. Diagnostic multiplex fondé sur les systèmes effecteurs crispr
US12534714B2 (en) 2019-03-18 2026-01-27 The Broad Institute, Inc. Type VII CRISPR proteins and systems
WO2020245785A2 (fr) * 2019-06-06 2020-12-10 Tata Consultancy Services Limited Procédé et système d'identification de sites cibles candidats pour lutter contre des agents pathogènes
US20220333208A1 (en) 2019-09-03 2022-10-20 The Broad Institute, Inc. Crispr effector system based multiplex cancer diagnostics
US11844800B2 (en) 2019-10-30 2023-12-19 Massachusetts Institute Of Technology Methods and compositions for predicting and preventing relapse of acute lymphoblastic leukemia
WO2021146641A1 (fr) 2020-01-17 2021-07-22 The Broad Institute, Inc. Protéines cas de type ii-d de petite taille et leurs procédés d'utilisation
EP4121532B1 (fr) 2020-03-17 2025-07-30 The Broad Institute, Inc. Systèmes et procédés de diagnostic à haut rendement de système crispr
WO2022040169A1 (fr) * 2020-08-17 2022-02-24 University Of Maryland, College Park Compositions, systèmes et procédés d'ingénierie génomique orthogonale chez la plante
IL308806A (en) 2021-06-01 2024-01-01 Arbor Biotechnologies Inc Gene editing systems including nuclease crisper and their uses
CN114592041A (zh) * 2022-03-04 2022-06-07 章毅 电化学检测外泌体的方法
EP4622662A1 (fr) 2022-11-23 2025-10-01 Amber Bio Inc. Endonucléases de modification génique
WO2024173645A1 (fr) 2023-02-15 2024-08-22 Arbor Biotechnologies, Inc. Procédé d'édition génique pour inhiber l'épissage aberrant du transcrit de la stathmine 2 (stmn2)
WO2025171210A1 (fr) 2024-02-09 2025-08-14 Arbor Biotechnologies, Inc. Compositions et procédés d'édition de gènes par l'intermédiaire d'une jonction d'extrémité à médiation par homologie
WO2026015829A2 (fr) 2024-07-12 2026-01-15 Arbor Biotechnologies, Inc. Petites transcriptases inverses et systèmes d'édition de gènes les comprenant
WO2026015832A2 (fr) 2024-07-12 2026-01-15 Arbor Biotechnologies, Inc. Transcriptases inverses et systèmes d'édition de gènes le comprenant

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WO2016205749A1 (fr) * 2015-06-18 2016-12-22 The Broad Institute Inc. Nouvelles enzymes crispr et systèmes associés
WO2016205764A1 (fr) * 2015-06-18 2016-12-22 The Broad Institute Inc. Nouvelles enzymes crispr et systèmes associés
WO2018107129A1 (fr) * 2016-12-09 2018-06-14 The Broad Institute, Inc. Diagnostics basés sur un système effecteur crispr

Patent Citations (3)

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Publication number Priority date Publication date Assignee Title
WO2016205749A1 (fr) * 2015-06-18 2016-12-22 The Broad Institute Inc. Nouvelles enzymes crispr et systèmes associés
WO2016205764A1 (fr) * 2015-06-18 2016-12-22 The Broad Institute Inc. Nouvelles enzymes crispr et systèmes associés
WO2018107129A1 (fr) * 2016-12-09 2018-06-14 The Broad Institute, Inc. Diagnostics basés sur un système effecteur crispr

Non-Patent Citations (9)

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Title
DATABASE GenPept [online] 28 June 2013 (2013-06-28), "hypothetical protein [Leptotrichia shahii], Cas13A_C2c2", XP093127598, retrieved from NCBI accession no. WP_018451595.1 Database accession no. WP_018451595.1 *
DATABASE NCBI [online] 4 June 2013 (2013-06-04), ANONYMOUS: "hypothetical protein [Fusobacterium necrophorum]", XP055975892, retrieved from NCBI accession no. WP_005959231.1 Database accession no. WP_005959231 *
DATABASE NCBI [online] NCBI; 28 September 2020 (2020-09-28), SHMAKOV SERGEY ET AL: "Type VI-C CRISPR-associated RNA-guided ribonuclease Cas13c [Fusobacterium necrophorum]", XP055975889, retrieved from NCBI accession no. WP_005959231.1 Database accession no. WP_005959231.1 *
DATABASE NCBI [online] NIH; 1 March 2017 (2017-03-01), SHMAKOV SERGEY ET AL: "type VI-C CRISPR-associated RNA-guided ribonuclease Cas13c [Fusobacterium perfoetens]", XP055975898, retrieved from NCBI accession no. WP_027128616.1 Database accession no. WP_027128616.1 *
DATABASE NCBI [online] NIH; 12 June 2014 (2014-06-12), ANONYMOUS: "hypothetical protein [Fusobacterium perfoetens]", XP055975899, retrieved from https://ncbi.nlm.nih.gov/protein/652854698?sat=48&satkey=113133233 accession no. WP_027128616.1 Database accession no. WP_027128616 *
See also references of WO2019005866A1 *
SERGEY SHMAKOV ET AL: "Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems", MOLECULAR CELL, vol. 60, no. 3, 1 November 2015 (2015-11-01), AMSTERDAM, NL, pages 385 - 397, XP055267512, ISSN: 1097-2765, DOI: 10.1016/j.molcel.2015.10.008 *
SHMAKOV SERGEY ET AL: "Diversity and evolution of class 2 CRISPR-Cas systems", NATURE REVIEWS. MICROBIOLOGY, NATURE PUBLISHING GROUP, ENGLAND, vol. 15, no. 3, 23 January 2017 (2017-01-23) - 1 March 2017 (2017-03-01), pages 169 - 182, XP002767857, ISSN: 1740-1534, DOI: 10.1038/NRMICRO.2016.184 *
SHMAKOV SERGEY ET AL: "Diversity and evolution of class 2 CRISPR-Cas systems", NATURE REVIEWS. MICROBIOLOGY, NATURE PUBLISHING GROUP, ENGLAND, vol. 15, no. 3, 23 January 2017 (2017-01-23), pages 169 - 182, XP002767857, ISSN: 1740-1534, DOI: 10.1038/NRMICRO.2016.184 *

Also Published As

Publication number Publication date
WO2019005866A1 (fr) 2019-01-03
EP3645728A1 (fr) 2020-05-06

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