EP3733936A4 - Ganzgenom-sgrna-bibliothek des schweines und verfahren zu ihrer herstellung und verwendung - Google Patents

Ganzgenom-sgrna-bibliothek des schweines und verfahren zu ihrer herstellung und verwendung Download PDF

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Publication number
EP3733936A4
EP3733936A4 EP18897080.0A EP18897080A EP3733936A4 EP 3733936 A4 EP3733936 A4 EP 3733936A4 EP 18897080 A EP18897080 A EP 18897080A EP 3733936 A4 EP3733936 A4 EP 3733936A4
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EP
European Patent Office
Prior art keywords
application
construction method
method therefor
whole genome
sgrna library
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP18897080.0A
Other languages
English (en)
French (fr)
Other versions
EP3733936A1 (de
Inventor
Pengyang XU
Fengdan XU
Guangyou DUAN
Wenbo MIN
Bugao XIA
Yi Ge
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genewiz Suzhou Ltd
Original Assignee
Genewiz Suzhou Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genewiz Suzhou Ltd filed Critical Genewiz Suzhou Ltd
Publication of EP3733936A1 publication Critical patent/EP3733936A1/de
Publication of EP3733936A4 publication Critical patent/EP3733936A4/de
Withdrawn legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B35/00—ICT specially adapted for in silico combinatorial libraries of nucleic acids, proteins or peptides
    • G16B35/10—Design of libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/12—Applications; Uses in screening processes in functional genomics, i.e. for the determination of gene function
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised
    • C12N2330/31—Libraries, arrays

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Library & Information Science (AREA)
  • Theoretical Computer Science (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Medical Informatics (AREA)
  • Evolutionary Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP18897080.0A 2017-12-29 2018-12-14 Ganzgenom-sgrna-bibliothek des schweines und verfahren zu ihrer herstellung und verwendung Withdrawn EP3733936A4 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201711490244.2A CN108221058A (zh) 2017-12-29 2017-12-29 一种猪全基因组sgRNA文库及其构建方法和应用
PCT/CN2018/121326 WO2019128743A1 (zh) 2017-12-29 2018-12-14 一种猪全基因组sgRNA文库及其构建方法和应用

Publications (2)

Publication Number Publication Date
EP3733936A1 EP3733936A1 (de) 2020-11-04
EP3733936A4 true EP3733936A4 (de) 2022-01-05

Family

ID=62646440

Family Applications (1)

Application Number Title Priority Date Filing Date
EP18897080.0A Withdrawn EP3733936A4 (de) 2017-12-29 2018-12-14 Ganzgenom-sgrna-bibliothek des schweines und verfahren zu ihrer herstellung und verwendung

Country Status (3)

Country Link
EP (1) EP3733936A4 (de)
CN (1) CN108221058A (de)
WO (1) WO2019128743A1 (de)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108221058A (zh) * 2017-12-29 2018-06-29 苏州金唯智生物科技有限公司 一种猪全基因组sgRNA文库及其构建方法和应用
CN108118058B (zh) 2017-12-29 2021-06-29 苏州金唯智生物科技有限公司 一种改进的启动子及其应用
CN108118059B (zh) 2017-12-30 2021-03-19 苏州金唯智生物科技有限公司 一种改进的启动子及其组成的载体和应用
CN109817340B (zh) * 2019-01-16 2023-06-23 苏州金唯智生物科技有限公司 疾病风险分布信息确定方法、装置、存储介质及设备
CN109957842B (zh) * 2019-02-11 2022-06-24 王纪武 一种用模块化设计构建双系统表达质粒文库的方法
CN110358766A (zh) * 2019-06-10 2019-10-22 云南农业大学 一种基于全基因组CRISPR/Cas9文库筛选异种移植抗原基因的方法
CN113436683B (zh) * 2020-03-23 2024-08-16 北京合生基因科技有限公司 筛选候选插入片段的方法和系统
CN111733159B (zh) * 2020-06-01 2022-09-27 五邑大学 用于猪MBP基因敲除的sgRNA组合物及用途
CN111849983A (zh) * 2020-07-17 2020-10-30 中国农业大学 一种sgRNA及其应用
CN112992277B (zh) * 2021-03-18 2021-10-26 南京先声医学检验实验室有限公司 一种微生物基因组数据库构建方法及其应用
GB202114206D0 (en) * 2021-10-04 2021-11-17 Genome Res Ltd Novel method
CN113990394B (zh) * 2021-10-27 2023-01-24 云舟生物科技(广州)股份有限公司 向导rna作用靶点的筛选方法、计算机存储介质及电子设备
CN114277447A (zh) * 2021-12-21 2022-04-05 翌圣生物科技(上海)股份有限公司 靶序列随机sgRNA全覆盖组的制备方法
CN115976653A (zh) * 2022-09-21 2023-04-18 江苏省农业科学院 一种创制西瓜全基因组突变体文库的方法和应用
CN116206684B (zh) * 2022-12-26 2024-01-30 纳昂达(南京)生物科技有限公司 一种评估基因组重复区探针捕获安全性的方法及其装置
CN118087051B (zh) * 2024-03-13 2025-02-11 内蒙古农业大学 基于绵羊全基因组CRISPR-Cas9敲除文库筛选绵羊卵泡颗粒细胞FSH应答基因

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5652095A (en) * 1992-09-24 1997-07-29 The Regents Of The University Of California Interferon regulatory factors 1 and 2 in the diagnosis of tumorigenicity
WO2015065964A1 (en) * 2013-10-28 2015-05-07 The Broad Institute Inc. Functional genomics using crispr-cas systems, compositions, methods, screens and applications thereof
CN105886616A (zh) * 2016-04-20 2016-08-24 广东省农业科学院农业生物基因研究中心 一种用于猪基因编辑的高效特异性sgRNA识别位点引导序列及其筛选方法
US20160272965A1 (en) * 2013-06-17 2016-09-22 Massachusetts Institute Of Technology Functional genomics using crispr-cas systems, compositions, methods, screens and applications thereof

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3001683C (en) * 2015-06-05 2024-06-04 The Regents Of The University Of California Methods and compositions for generating crispr/cas guide rnas
WO2017075529A1 (en) * 2015-10-29 2017-05-04 The Research Foundation For The State University Of New York Genomic combinatorial screening platform
CN106845151B (zh) * 2015-12-07 2019-03-26 中国农业大学 CRISPR-Cas9系统sgRNA作用靶点的筛选方法及装置
CN107365793A (zh) * 2017-06-19 2017-11-21 百格基因科技（江苏）有限公司 一种适用于植物的大规模基因组编辑的方法
CN108205614A (zh) * 2017-12-29 2018-06-26 苏州金唯智生物科技有限公司 一种全基因组sgRNA文库的构建系统及其应用
CN108221058A (zh) * 2017-12-29 2018-06-29 苏州金唯智生物科技有限公司 一种猪全基因组sgRNA文库及其构建方法和应用

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5652095A (en) * 1992-09-24 1997-07-29 The Regents Of The University Of California Interferon regulatory factors 1 and 2 in the diagnosis of tumorigenicity
US20160272965A1 (en) * 2013-06-17 2016-09-22 Massachusetts Institute Of Technology Functional genomics using crispr-cas systems, compositions, methods, screens and applications thereof
WO2015065964A1 (en) * 2013-10-28 2015-05-07 The Broad Institute Inc. Functional genomics using crispr-cas systems, compositions, methods, screens and applications thereof
CN105886616A (zh) * 2016-04-20 2016-08-24 广东省农业科学院农业生物基因研究中心 一种用于猪基因编辑的高效特异性sgRNA识别位点引导序列及其筛选方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
See also references of WO2019128743A1 *
WANG P W ET AL: "Refinement of the Smallest Commonly Deleted Segment of Chromosome 20 in Malignant Myeloid Diseases and Development of a PAC-Based Physical and Transcription Map", GENOMICS, ACADEMIC PRESS, SAN DIEGO, US, vol. 67, no. 1, 1 July 2000 (2000-07-01), pages 28 - 39, XP004439321, ISSN: 0888-7543, DOI: 10.1006/GENO.2000.6215 *

Also Published As

Publication number Publication date
EP3733936A1 (de) 2020-11-04
WO2019128743A1 (zh) 2019-07-04
CN108221058A (zh) 2018-06-29

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