EP3821000A4 - Enzymes variantes de galactose oxydase obtenues par génie génétique - Google Patents

Enzymes variantes de galactose oxydase obtenues par génie génétique Download PDF

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Publication number
EP3821000A4
EP3821000A4 EP19835169.4A EP19835169A EP3821000A4 EP 3821000 A4 EP3821000 A4 EP 3821000A4 EP 19835169 A EP19835169 A EP 19835169A EP 3821000 A4 EP3821000 A4 EP 3821000A4
Authority
EP
European Patent Office
Prior art keywords
genetic engineering
galactose oxidase
enzymes obtained
variant enzymes
oxidase variant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP19835169.4A
Other languages
German (de)
English (en)
Other versions
EP3821000A1 (fr
Inventor
Christopher Michael MICKLITSCH
Oscar Alvizo
Jovana Nazor
Harvinder Chagger MANIAR
Mikayla Jianghongxia KRAWCZYK
Margie Tabuga BORRA-GARSKE
Nandhitha Subramanian
Anna FRYSZKOWSKA
Nicholas M. MARSHALL
Agustina RODRIGUEZ-GRANILLO
Deeptak Verma
Dewan ANDREWS
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Codexis Inc
Original Assignee
Codexis Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Codexis Inc filed Critical Codexis Inc
Publication of EP3821000A1 publication Critical patent/EP3821000A1/fr
Publication of EP3821000A4 publication Critical patent/EP3821000A4/fr
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/03—Oxidoreductases acting on the CH-OH group of donors (1.1) with a oxygen as acceptor (1.1.3)
    • C12Y101/03009—Galactose oxidase (1.1.3.9)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
EP19835169.4A 2018-07-09 2019-07-02 Enzymes variantes de galactose oxydase obtenues par génie génétique Pending EP3821000A4 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862695570P 2018-07-09 2018-07-09
US201962822286P 2019-03-22 2019-03-22
PCT/US2019/040376 WO2020014049A1 (fr) 2018-07-09 2019-07-02 Enzymes variantes de galactose oxydase obtenues par génie génétique

Publications (2)

Publication Number Publication Date
EP3821000A1 EP3821000A1 (fr) 2021-05-19
EP3821000A4 true EP3821000A4 (fr) 2022-07-20

Family

ID=69101280

Family Applications (1)

Application Number Title Priority Date Filing Date
EP19835169.4A Pending EP3821000A4 (fr) 2018-07-09 2019-07-02 Enzymes variantes de galactose oxydase obtenues par génie génétique

Country Status (11)

Country Link
US (4) US11466259B2 (fr)
EP (1) EP3821000A4 (fr)
JP (2) JP7491587B2 (fr)
KR (1) KR20210030379A (fr)
CN (1) CN112673097B (fr)
AU (1) AU2019300837B2 (fr)
CA (1) CA3103514A1 (fr)
IL (1) IL279883B2 (fr)
MX (1) MX2021000328A (fr)
SG (1) SG11202012138XA (fr)
WO (1) WO2020014049A1 (fr)

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BR112021000202B1 (pt) 2018-07-09 2023-03-28 Merck Sharp & Dohme Llc Métodos para síntese enzimática de nucleosídeo 4'-etinila 2-desóxi e compostos
EP3821000A4 (fr) 2018-07-09 2022-07-20 Codexis, Inc. Enzymes variantes de galactose oxydase obtenues par génie génétique
IL319918A (en) * 2018-07-30 2025-05-01 Tate & Lyle Solutions Usa Llc Engineered glycosyltransferases and methods for glycosylation of steviol glycoside
EP3943598A1 (fr) * 2020-07-21 2022-01-26 Institut national de recherche pour l'agriculture l'alimentation et l'environnement Utilisation d'enzymes activés par les uv pour mettre en oeuvre des réactions d'oxydation et les processus correspondants
WO2022076263A1 (fr) * 2020-10-06 2022-04-14 Codexis, Inc. Enzymes variantes de galactose oxydase modifiées
US20260055379A1 (en) * 2022-08-11 2026-02-26 Agency For Science, Technology And Research An Improved Galactose Oxidase Enzyme Panel for Oxidation of Secondary Alcohols
CN116355872B (zh) * 2023-02-23 2023-09-01 中国水产科学研究院黄海水产研究所 半乳糖氧化酶突变体gao-5f/ar、质粒、重组菌及其应用
WO2025098128A1 (fr) * 2023-11-06 2025-05-15 Enzymaster (Ningbo) Bio-Engineering Co., Ltd. Procédé de synthèse d'acide glucarique et enzymes utilisées à cet effet
CN117230032B (zh) * 2023-11-16 2024-01-30 中国水产科学研究院黄海水产研究所 半乳糖氧化酶突变体gao-ar/ht、基因、质粒、重组菌及其应用
CN118773166B (zh) * 2024-09-05 2025-11-18 无锡蔚蓝生物科技有限公司 一种脂肪酶突变体及其在油脂加工中的应用
CN119242604B (zh) * 2024-12-06 2025-04-25 山东理工大学 一种半乳糖氧化酶突变体及其在制备2-氧代-2-呋喃基乙酸中应用

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WO2003072742A2 (fr) * 2002-02-27 2003-09-04 California Institute Of Technology Nouvelles glucose 6-oxydases

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Also Published As

Publication number Publication date
US11466259B2 (en) 2022-10-11
JP7491587B2 (ja) 2024-05-28
US20200010869A1 (en) 2020-01-09
MX2021000328A (es) 2021-03-25
WO2020014049A1 (fr) 2020-01-16
SG11202012138XA (en) 2021-01-28
IL279883B2 (en) 2026-06-01
US12012618B2 (en) 2024-06-18
JP2024042079A (ja) 2024-03-27
KR20210030379A (ko) 2021-03-17
US20230026007A1 (en) 2023-01-26
US20260043009A1 (en) 2026-02-12
IL279883A (en) 2021-03-01
IL279883B1 (en) 2026-02-01
CA3103514A1 (fr) 2020-01-16
US20240287474A1 (en) 2024-08-29
CN112673097A (zh) 2021-04-16
CN112673097B (zh) 2025-05-06
AU2019300837B2 (en) 2025-05-22
US12480099B2 (en) 2025-11-25
AU2019300837A1 (en) 2021-01-07
JP2021530982A (ja) 2021-11-18
EP3821000A1 (fr) 2021-05-19

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