EP3947667A4 - Modifizierte cleavase, verwendungen davon und zugehörige kits - Google Patents

Modifizierte cleavase, verwendungen davon und zugehörige kits Download PDF

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Publication number
EP3947667A4
EP3947667A4 EP20779890.1A EP20779890A EP3947667A4 EP 3947667 A4 EP3947667 A4 EP 3947667A4 EP 20779890 A EP20779890 A EP 20779890A EP 3947667 A4 EP3947667 A4 EP 3947667A4
Authority
EP
European Patent Office
Prior art keywords
cleavases
modified
related kits
kits
modified cleavases
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP20779890.1A
Other languages
English (en)
French (fr)
Other versions
EP3947667A1 (de
Inventor
Kevin Desai
Kevin L. GUNDERSON
Robert C. James
Lei Shi
Stephen VERESPY, III
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Encodia Inc
Original Assignee
Encodia Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Encodia Inc filed Critical Encodia Inc
Publication of EP3947667A1 publication Critical patent/EP3947667A1/de
Publication of EP3947667A4 publication Critical patent/EP3947667A4/de
Withdrawn legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/485—Exopeptidases (3.4.11-3.4.19)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/14—Dipeptidyl-peptidases and tripeptidyl-peptidases (3.4.14)
    • C12Y304/14004—Dipeptidyl-peptidase III (3.4.14.4)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP20779890.1A 2019-03-26 2020-03-24 Modifizierte cleavase, verwendungen davon und zugehörige kits Withdrawn EP3947667A4 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201962824157P 2019-03-26 2019-03-26
US201962823927P 2019-03-26 2019-03-26
US201962931737P 2019-11-06 2019-11-06
PCT/US2020/024521 WO2020198264A1 (en) 2019-03-26 2020-03-24 Modified cleavases, uses thereof and related kits

Publications (2)

Publication Number Publication Date
EP3947667A1 EP3947667A1 (de) 2022-02-09
EP3947667A4 true EP3947667A4 (de) 2022-12-28

Family

ID=72612078

Family Applications (1)

Application Number Title Priority Date Filing Date
EP20779890.1A Withdrawn EP3947667A4 (de) 2019-03-26 2020-03-24 Modifizierte cleavase, verwendungen davon und zugehörige kits

Country Status (8)

Country Link
EP (1) EP3947667A4 (de)
JP (1) JP2022526939A (de)
KR (1) KR102567902B1 (de)
CN (1) CN113993997A (de)
AU (1) AU2020247918B2 (de)
MX (1) MX2021011726A (de)
SG (1) SG11202110373WA (de)
WO (1) WO2020198264A1 (de)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7390027B2 (ja) 2017-10-31 2023-12-01 エンコディア， インコーポレイテッド 核酸エンコーディングおよび／または標識を使用する解析のためのキット
WO2021194908A1 (en) 2020-03-24 2021-09-30 Encodia, Inc. Modified dipeptide cleavases, uses thereof and related kits
US11427814B2 (en) 2019-03-26 2022-08-30 Encodia, Inc. Modified cleavases, uses thereof and related kits
WO2020223133A1 (en) * 2019-04-30 2020-11-05 Encodia, Inc. Methods and reagents for cleavage of the n-terminal amino acid from a polypeptide
WO2021141924A1 (en) 2020-01-07 2021-07-15 Encodia, Inc. Methods for stable complex formation and related kits
EP4196581B1 (de) 2020-08-19 2026-02-25 Encodia, Inc. Sequenzielle codierungsverfahren und zugehörige kits
EP4271997A4 (de) * 2020-12-31 2024-12-04 Encodia, Inc. Metalloenzyme zur biomolekularen erkennung n-terminal modifizierter peptide
US20250163112A1 (en) * 2021-08-02 2025-05-22 Spiber Inc. Porous Body and Method for Producing Same
CN114460052B (zh) * 2022-01-11 2023-06-20 武汉理工大学 一种基于荧光碳量子点直接检测丙酮酸钠浓度的方法
CN116426509B (zh) * 2023-04-27 2024-07-30 湖北佶凯星生物科技有限公司 一种碱性蛋白酶组合突变体及其应用
WO2026030423A1 (en) * 2024-07-31 2026-02-05 Glyphic Biotechnologies, Inc. Sequencing reagents using guanidinylating agents

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0595476A2 (de) * 1992-10-01 1994-05-04 Eli Lilly And Company Dipeptidylaminopeptidase von Dictyostelium
WO2010065322A1 (en) * 2008-12-01 2010-06-10 Research Triangle Institute Concurrent identification of multitudes of polypeptides
WO2017192633A1 (en) * 2016-05-02 2017-11-09 Procure Life Sciences Inc. Macromolecule analysis employing nucleic acid encoding
US20210214701A1 (en) * 2019-03-26 2021-07-15 Encodia, Inc. Modified cleavases, uses thereof and related kits
WO2021194908A1 (en) * 2020-03-24 2021-09-30 Encodia, Inc. Modified dipeptide cleavases, uses thereof and related kits

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6875851B1 (en) * 1999-03-05 2005-04-05 University Of Georgia Research Foundation, Inc. Prolyl tripeptidyl peptidases nucleic acid of Porphyromonas gingivalis
PL366005A1 (en) * 2000-10-12 2005-01-24 Ferring Bv Novel serine protease genes related to dppiv
MX282672B (es) * 2002-10-10 2011-01-10 Diversa Corp Proteasas, acidos nucleicos que los codifican, y metodos de hacer y usar los mismos.
WO2005015239A2 (en) * 2003-08-07 2005-02-17 Cornell Research Foundation, Inc. Method for n-terminal labeling of proteins
EP1972349A1 (de) * 2007-03-21 2008-09-24 Biocompatibles UK Limited Mit Polymer(en) konjugierte GLP-1-Fusionspeptide, ihre Herstellung und Verwendung
EP1975176A1 (de) * 2007-03-27 2008-10-01 Biocompatibles UK Limited Neue GLP-1-Fusionspeptide, ihre Herstellung und Verwendung
EP2034020A1 (de) * 2007-08-30 2009-03-11 Procomcure Biotech GmbH Verfahren zur Herstellung eines modifizierten Peptids
CA3111953C (en) * 2011-04-05 2023-10-24 Cellectis Method for the generation of compact tale-nucleases and uses thereof
CN104350149A (zh) * 2012-01-26 2015-02-11 诺维信公司 具有蛋白酶活性的多肽在动物饲料和洗涤剂中的用途
US9435810B2 (en) * 2013-03-15 2016-09-06 Washington University Molecules and methods for iterative polypeptide analysis and processing

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0595476A2 (de) * 1992-10-01 1994-05-04 Eli Lilly And Company Dipeptidylaminopeptidase von Dictyostelium
WO2010065322A1 (en) * 2008-12-01 2010-06-10 Research Triangle Institute Concurrent identification of multitudes of polypeptides
WO2017192633A1 (en) * 2016-05-02 2017-11-09 Procure Life Sciences Inc. Macromolecule analysis employing nucleic acid encoding
US20210214701A1 (en) * 2019-03-26 2021-07-15 Encodia, Inc. Modified cleavases, uses thereof and related kits
WO2021194908A1 (en) * 2020-03-24 2021-09-30 Encodia, Inc. Modified dipeptide cleavases, uses thereof and related kits

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BORGO BENJAMIN ET AL: "Computer-aided design of a catalyst for Edman degradation utilizing substrate-assisted catalysis", PROTEIN SCIENCE, vol. 24, no. 4, 30 April 2015 (2015-04-30), US, pages 571 - 579, XP093135294, ISSN: 0961-8368, DOI: 10.1002/pro.2633 *
OGASAWARA W ET AL: "Two types of novel dipeptidyl aminopeptidases from Pseudomonas sp. strain WO24", JOURNAL OF BACTERIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 178, no. 21, 1 November 1996 (1996-11-01), pages 6288 - 6295, XP002219378, ISSN: 0021-9193 *
SAKAMOTO YASUMITSU ET AL: "S46 Peptidases are the First Exopeptidases to be Members of Clan PA", SCIENTIFIC REPORTS, vol. 4, no. 1, 1 May 2015 (2015-05-01), XP055974089, DOI: 10.1038/srep04977 *
See also references of WO2020198264A1 *
SUZUKI YOSHIYUKI ET AL: "Identification of the Catalytic Triad of Family S46 Exopeptidases, Closely Related to Clan PA Endopeptidases", SCIENTIFIC REPORTS, vol. 4, no. 1, 1 May 2015 (2015-05-01), XP055974091, DOI: 10.1038/srep04292 *

Also Published As

Publication number Publication date
WO2020198264A8 (en) 2021-01-07
KR102567902B1 (ko) 2023-08-18
CN113993997A (zh) 2022-01-28
JP2022526939A (ja) 2022-05-27
SG11202110373WA (en) 2021-10-28
AU2020247918A1 (en) 2021-10-21
WO2020198264A1 (en) 2020-10-01
EP3947667A1 (de) 2022-02-09
CA3134776A1 (en) 2020-10-01
AU2020247918B2 (en) 2022-06-30
KR20210143236A (ko) 2021-11-26
MX2021011726A (es) 2021-10-22

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