EP3976064A4 - Procédés et appareils pour des micro-organosphères dérivées d'un patient - Google Patents

Procédés et appareils pour des micro-organosphères dérivées d'un patient Download PDF

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Publication number
EP3976064A4
EP3976064A4 EP20812521.1A EP20812521A EP3976064A4 EP 3976064 A4 EP3976064 A4 EP 3976064A4 EP 20812521 A EP20812521 A EP 20812521A EP 3976064 A4 EP3976064 A4 EP 3976064A4
Authority
EP
European Patent Office
Prior art keywords
organospheres
drived
patient
micro
methods
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP20812521.1A
Other languages
German (de)
English (en)
Other versions
EP3976064A1 (fr
Inventor
Xiling Shen
Zhaouhui WANG
Daniel DELUBAC
Jeffrey MOTSCHMAN
Dvid HSU
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Duke University
Xilis Inc
Original Assignee
Duke University
Xilis Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Duke University, Xilis Inc filed Critical Duke University
Publication of EP3976064A1 publication Critical patent/EP3976064A1/fr
Publication of EP3976064A4 publication Critical patent/EP3976064A4/fr
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/01—Drops
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/08—Chemical, biochemical or biological means, e.g. plasma jet, co-culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0012—Cell encapsulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0668—Mesenchymal stem cells from other natural sources
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/16—Particles; Beads; Granular material; Encapsulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/50—Soluble polymers, e.g. polyethyleneglycol [PEG]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Immunology (AREA)
  • Oncology (AREA)
  • Rheumatology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physiology (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
EP20812521.1A 2019-05-28 2020-04-01 Procédés et appareils pour des micro-organosphères dérivées d'un patient Pending EP3976064A4 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962853219P 2019-05-28 2019-05-28
PCT/US2020/026275 WO2020242594A1 (fr) 2019-05-28 2020-04-01 Procédés et appareils pour des micro-organosphères dérivées d'un patient

Publications (2)

Publication Number Publication Date
EP3976064A1 EP3976064A1 (fr) 2022-04-06
EP3976064A4 true EP3976064A4 (fr) 2023-05-31

Family

ID=73549270

Family Applications (1)

Application Number Title Priority Date Filing Date
EP20812521.1A Pending EP3976064A4 (fr) 2019-05-28 2020-04-01 Procédés et appareils pour des micro-organosphères dérivées d'un patient

Country Status (10)

Country Link
US (2) US11555180B2 (fr)
EP (1) EP3976064A4 (fr)
JP (1) JP2022534778A (fr)
CN (1) CN116157134A (fr)
AU (1) AU2020283390A1 (fr)
CA (1) CA3142113A1 (fr)
IL (1) IL288370A (fr)
MX (1) MX2021014439A (fr)
SG (1) SG11202113122SA (fr)
WO (1) WO2020242594A1 (fr)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114410466A (zh) * 2020-04-01 2022-04-29 沈西凌 用于个性化癌症治疗的精确药物筛选
WO2022115455A1 (fr) * 2020-11-24 2022-06-02 Duke University Dosages immuno-oncologiques à base d'organoïdes en gouttelettes et méthodes associées d'utilisation
DE102022100146B4 (de) 2022-01-04 2023-11-30 Precomb Therapeutics Ag Verfahren zur analyse von personalisierten anti-krebs-wirkstoffen in zellkulturen
JP2025514291A (ja) * 2022-04-29 2025-05-02 ザイリス， インク． マイクロオルガノスフェアを使用して肝細胞毒性を試験するための方法及び装置
EP4519686A4 (fr) * 2022-05-03 2026-05-06 Xilis Inc Micro-organosphères (mos) dérivées d'un patient permettant une oncologie de précision clinique
WO2024039770A2 (fr) * 2022-08-17 2024-02-22 Xilis, Inc. Dosages de médicament de micro-organosphère basés sur l'imagerie
JP2025532497A (ja) * 2022-09-07 2025-10-01 ザイリス， インク． 多発性骨髄腫ＭｉｃｒｏＯｒｇａｎｏＳｐｈｅｒｅ
EP4601758A1 (fr) 2022-10-11 2025-08-20 Xilis, Inc. Système microfluidique intégré pour la génération de micro-organosphères (mos)

Citations (2)

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Publication number Priority date Publication date Assignee Title
WO2019010587A1 (fr) * 2017-07-14 2019-01-17 The Governing Council Of The University Of Toronto Plate-forme microfluidique pour la production rapide d'organoïdes/sphéroïdes pour criblage de composés
WO2020215487A1 (fr) * 2019-04-22 2020-10-29 清华-伯克利深圳学院筹备办公室 Procédé de préparation d'un sphéroïde organoïde

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PT671923E (pt) 1992-10-09 2001-10-30 Advanced Tissue Sciences Inc Celulas de reserva do figado
WO2005047863A2 (fr) 2003-11-10 2005-05-26 Platypus Technologies, Llc Substrats, dispositifs et procedes de dosages cellulaires
US7759111B2 (en) * 2004-08-27 2010-07-20 The Regents Of The University Of California Cell encapsulation microfluidic device
WO2006078814A2 (fr) 2005-01-20 2006-07-27 The Regents Of The University Of California Microreseaux cellulaires pour le criblage de facteurs de differenciation
US20070134209A1 (en) * 2005-12-12 2007-06-14 Metafluidics, Inc. Cellular encapsulation for self-assembly of engineered tissue
US20120135875A1 (en) * 2008-02-29 2012-05-31 Bristol-Myers Squibb Company Methods for determining sensitivity to vascular endothelial growth factor receptor-2 modulators
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WO2012018982A2 (fr) * 2010-08-05 2012-02-09 Tufts Medical Center, Inc. Procédés et trousses de modulation de l'invasivité tumorale et du potentiel métastatique
GB201105226D0 (en) * 2011-03-29 2011-05-11 Univ Leiden Methods
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US9334473B2 (en) 2012-08-17 2016-05-10 Jelena Vukasinovic Three dimensional cell culture compositions and methods of use
WO2014082096A1 (fr) * 2012-11-26 2014-05-30 The Trustees Of Columbia University In The City Of New York Procédé pour la culture d'organoïdes de prostate humaine et murine et utilisations correspondantes
KR102257305B1 (ko) 2013-03-28 2021-05-28 더 유니버시티 오브 브리티쉬 콜롬비아 미세유동 장치 및 분비물의 다세포 검정법에서의 그의 사용 방법
AU2014275006A1 (en) * 2013-06-04 2015-12-24 University Of Miami Assays, methods and kits for analyzing sensitivity and resistance to anti-cancer drugs, predicting a cancer patient's prognosis, and personalized treatment strategies
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WO2020215487A1 (fr) * 2019-04-22 2020-10-29 清华-伯克利深圳学院筹备办公室 Procédé de préparation d'un sphéroïde organoïde

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See also references of WO2020242594A1 *

Also Published As

Publication number Publication date
EP3976064A1 (fr) 2022-04-06
US11555180B2 (en) 2023-01-17
JP2022534778A (ja) 2022-08-03
AU2020283390A1 (en) 2021-12-23
IL288370A (en) 2022-01-01
MX2021014439A (es) 2022-05-19
US20200377861A1 (en) 2020-12-03
CN116157134A (zh) 2023-05-23
SG11202113122SA (en) 2021-12-30
WO2020242594A1 (fr) 2020-12-03
CA3142113A1 (fr) 2020-12-03
US20230002737A1 (en) 2023-01-05

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