EP4006134A4 - Kulturverfahren und kulturbehälter - Google Patents

Kulturverfahren und kulturbehälter Download PDF

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Publication number
EP4006134A4
EP4006134A4 EP20843314.4A EP20843314A EP4006134A4 EP 4006134 A4 EP4006134 A4 EP 4006134A4 EP 20843314 A EP20843314 A EP 20843314A EP 4006134 A4 EP4006134 A4 EP 4006134A4
Authority
EP
European Patent Office
Prior art keywords
culture
vessel
culture vessel
culture method
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP20843314.4A
Other languages
English (en)
French (fr)
Other versions
EP4006134A1 (de
Inventor
Jiro Kawada
Akira Kasai
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Jiksak Bioengineering Inc
Original Assignee
Jiksak Bioengineering Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jiksak Bioengineering Inc filed Critical Jiksak Bioengineering Inc
Publication of EP4006134A1 publication Critical patent/EP4006134A1/de
Publication of EP4006134A4 publication Critical patent/EP4006134A4/de
Withdrawn legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/34—Internal compartments or partitions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/10—Hollow fibers or tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/08—Chemical, biochemical or biological means, e.g. plasma jet, co-culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0619—Neurons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0658—Skeletal muscle cells, e.g. myocytes, myotubes, myoblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/02—Atmosphere, e.g. low oxygen conditions

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Sustainable Development (AREA)
  • Rheumatology (AREA)
  • Neurology (AREA)
  • Clinical Laboratory Science (AREA)
  • Immunology (AREA)
  • Neurosurgery (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Physiology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
EP20843314.4A 2019-07-22 2020-07-21 Kulturverfahren und kulturbehälter Withdrawn EP4006134A4 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2019134262 2019-07-22
JP2020034347 2020-02-28
PCT/JP2020/028338 WO2021015213A1 (ja) 2019-07-22 2020-07-21 培養方法および培養容器

Publications (2)

Publication Number Publication Date
EP4006134A1 EP4006134A1 (de) 2022-06-01
EP4006134A4 true EP4006134A4 (de) 2023-10-04

Family

ID=74194216

Family Applications (1)

Application Number Title Priority Date Filing Date
EP20843314.4A Withdrawn EP4006134A4 (de) 2019-07-22 2020-07-21 Kulturverfahren und kulturbehälter

Country Status (4)

Country Link
US (1) US20220259567A1 (de)
EP (1) EP4006134A4 (de)
JP (1) JP7624179B2 (de)
WO (1) WO2021015213A1 (de)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7685182B2 (ja) * 2020-10-29 2025-05-29 慶應義塾 人工軸索束
JP2024142686A (ja) * 2023-03-30 2024-10-11 ウシオ電機株式会社 神経細胞培養チップ

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006037033A2 (en) * 2004-09-24 2006-04-06 The Regents Of The University Of California A microfluidic device for enabling the controlled growth of cells
WO2015092144A1 (en) * 2013-12-20 2015-06-25 Tampereen Yliopisto A cell culturing platform, a cell culture system, and a method for modeling a neurovascular unit in vitro
EP3450540A1 (de) * 2016-04-28 2019-03-06 The Foundation for the Promotion of Industrial Science Vorrichtung zur kultivierung von nervenzellen, verfahren zur kultivierung von nervenzellen, kultivierte nervenzelle, verfahren zur analyse und identifizierung von protein in axonbündel und verfahren zur verwendung der nervenzelle

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2937050A1 (fr) * 2008-10-10 2010-04-16 Inst Curie Dispositif de culture cellulaire
US20100323434A1 (en) * 2009-06-19 2010-12-23 In Hong Yang Compartmentalized Nerve Culture System
JP2013021946A (ja) * 2011-07-19 2013-02-04 Shiseido Co Ltd 皮膚刺激評価用ｉｎｖｉｔｒｏ皮膚モデル組成物及びそれを用いた評価方法
JP5904789B2 (ja) * 2011-12-28 2016-04-20 株式会社 資生堂 末梢神経細胞とケラチノサイトの共培養系及びその使用方法
WO2017100705A1 (en) 2015-12-09 2017-06-15 Robert John Petcavich Mammalian cell in vitro topological neuron network
US10767149B2 (en) * 2016-06-13 2020-09-08 Massachusetts Institute Of Technology Microfluidic device for three dimensional and compartmentalized coculture of neuronal and muscle cells, with functional force readout
JP7151966B2 (ja) * 2017-06-14 2022-10-12 一般財団法人生産技術研究奨励会 神経細胞を培養する装置、神経細胞を培養する方法、軸索束の形態変性を配向性解析によって数値化する方法、神経組織、軸索束内のプロテインを解析及び同定する方法並びに神経細胞の使用方法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006037033A2 (en) * 2004-09-24 2006-04-06 The Regents Of The University Of California A microfluidic device for enabling the controlled growth of cells
WO2015092144A1 (en) * 2013-12-20 2015-06-25 Tampereen Yliopisto A cell culturing platform, a cell culture system, and a method for modeling a neurovascular unit in vitro
EP3450540A1 (de) * 2016-04-28 2019-03-06 The Foundation for the Promotion of Industrial Science Vorrichtung zur kultivierung von nervenzellen, verfahren zur kultivierung von nervenzellen, kultivierte nervenzelle, verfahren zur analyse und identifizierung von protein in axonbündel und verfahren zur verwendung der nervenzelle

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CECILIA A BRUNELLO ET AL: "Microtechnologies to fuel neurobiological research with nanometer precision", JOURNAL OF NANOBIOTECHNOLOGY, BIOMED CENTRAL, vol. 11, no. 1, 10 April 2013 (2013-04-10), pages 11, XP021144228, ISSN: 1477-3155, DOI: 10.1186/1477-3155-11-11 *
See also references of WO2021015213A1 *

Also Published As

Publication number Publication date
JPWO2021015213A1 (de) 2021-01-28
EP4006134A1 (de) 2022-06-01
WO2021015213A1 (ja) 2021-01-28
US20220259567A1 (en) 2022-08-18
JP7624179B2 (ja) 2025-01-30

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