EP4023767A4 - Procédé, composition et kit pour la pcr quantitative par fluorescence, et leur utilisation - Google Patents

Procédé, composition et kit pour la pcr quantitative par fluorescence, et leur utilisation Download PDF

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Publication number
EP4023767A4
EP4023767A4 EP20943941.3A EP20943941A EP4023767A4 EP 4023767 A4 EP4023767 A4 EP 4023767A4 EP 20943941 A EP20943941 A EP 20943941A EP 4023767 A4 EP4023767 A4 EP 4023767A4
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EP
European Patent Office
Prior art keywords
fluorescence
kit
composition
quantitative pcr
pcr
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP20943941.3A
Other languages
German (de)
English (en)
Other versions
EP4023767A1 (fr
EP4023767C0 (fr
EP4023767B1 (fr
Inventor
Lizhong Dai
Bozhi JI
Deyong TAN
Jia Liu
Xu FAN
Zhongping DENG
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sansure Biotech Inc
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Sansure Biotech Inc
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Publication date
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Publication of EP4023767A1 publication Critical patent/EP4023767A1/fr
Publication of EP4023767A4 publication Critical patent/EP4023767A4/fr
Application granted granted Critical
Publication of EP4023767C0 publication Critical patent/EP4023767C0/fr
Publication of EP4023767B1 publication Critical patent/EP4023767B1/fr
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/137—Concentration of a component of medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2545/00—Reactions characterised by their quantitative nature
    • C12Q2545/10—Reactions characterised by their quantitative nature the purpose being quantitative analysis
    • C12Q2545/114—Reactions characterised by their quantitative nature the purpose being quantitative analysis involving a quantitation step
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP20943941.3A 2020-07-08 2020-10-14 Procédé, composition et kit pour la pcr quantitative par fluorescence, et leur utilisation Active EP4023767B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN202010649826.6A CN111534576B (zh) 2020-07-08 2020-07-08 用于荧光定量pcr的方法、组合物、试剂盒及其用途
PCT/CN2020/120819 WO2022007224A1 (fr) 2020-07-08 2020-10-14 Procédé, composition et kit pour la pcr quantitative par fluorescence, et leur utilisation

Publications (4)

Publication Number Publication Date
EP4023767A1 EP4023767A1 (fr) 2022-07-06
EP4023767A4 true EP4023767A4 (fr) 2022-12-21
EP4023767C0 EP4023767C0 (fr) 2025-04-02
EP4023767B1 EP4023767B1 (fr) 2025-04-02

Family

ID=71974730

Family Applications (1)

Application Number Title Priority Date Filing Date
EP20943941.3A Active EP4023767B1 (fr) 2020-07-08 2020-10-14 Procédé, composition et kit pour la pcr quantitative par fluorescence, et leur utilisation

Country Status (5)

Country Link
US (1) US20230035871A1 (fr)
EP (1) EP4023767B1 (fr)
CN (1) CN111534576B (fr)
BR (1) BR112022022596A2 (fr)
WO (1) WO2022007224A1 (fr)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111534576B (zh) * 2020-07-08 2020-10-02 圣湘生物科技股份有限公司 用于荧光定量pcr的方法、组合物、试剂盒及其用途
CN113151583A (zh) * 2020-12-29 2021-07-23 苏州新波生物技术有限公司 一种可直接扩增含SARS CoV-2病毒样本的检测试剂盒及其检测方法
CN113528706A (zh) * 2021-07-13 2021-10-22 苏州博腾生物制药有限公司 三通道双淬灭探针检测可复制性慢病毒的试剂及其检测方法
CN113652493A (zh) * 2021-08-17 2021-11-16 厦门市妇幼保健院（厦门市计划生育服务中心） 一种能减少三种荧光通道pcr竞争的生殖道pcr体系
CN113718045B (zh) * 2021-09-18 2023-03-21 上海伯杰医疗科技股份有限公司北京分公司 用于检测4种鲍特菌和特异性检测百日咳鲍特菌的dna片段、引物、探针和试剂盒及应用
CN114214465A (zh) * 2022-01-26 2022-03-22 山东仕达思生物产业有限公司 一种缩短新型冠状病毒检测时间的引物、探针、试剂盒及检测方法
CN116121454A (zh) * 2022-12-09 2023-05-16 山东信达基因科技有限公司 猪瘟、猪繁殖与呼吸综合征及猪伪狂犬病联合检测试剂
CN116606958A (zh) * 2023-03-06 2023-08-18 广州科方生物技术股份有限公司 检测副流感1/2/3型的引物探针组合物、试剂盒及使用方法

Citations (3)

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WO2011120049A1 (fr) * 2010-03-26 2011-09-29 Integrated Dna Technologies, Inc. Procédés pour améliorer l'hybridation des acides nucléiques
WO2016168750A1 (fr) * 2015-04-15 2016-10-20 Biosearch Technologies, Inc. Sondes à désactivateur dual
CN110982876A (zh) * 2020-03-04 2020-04-10 圣湘生物科技股份有限公司 病毒核酸检测的预处理方法、预处理液、试剂盒及其用途

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US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
BR112014024023A2 (pt) * 2012-03-28 2017-07-18 Genentech Inc anticorpos idiotípicos anti-hcmv e usos dos mesmos
EP3234192B1 (fr) * 2014-12-19 2021-07-14 The Broad Institute, Inc. Identification non biaisée de cassures bicaténaires et réarrangement génomique par séquençage de capture d'insert à l'échelle du génome
CN104962608B (zh) * 2015-05-28 2017-11-07 广东省生态环境与土壤研究所 一种基于双淬灭基团核酸自组装技术的双酚a检测方法及检测试剂盒
EP3438258B1 (fr) * 2016-03-30 2021-09-22 FUJIFILM Wako Pure Chemical Corporation Jeu d'amorce pour la détection de chlamydia trachomatis, méthode de détection de chlamydia trachomatis utilisant ce dernier, et kit de réactifs associé
US11198903B2 (en) * 2016-09-15 2021-12-14 Roche Molecular Systems, Inc. Methods for performing multiplexed real-time PCR
CN107236815A (zh) * 2017-07-18 2017-10-10 江西贤聚景欣医药生物科技有限公司 用于靶核酸序列检测的多重淬灭荧光探针及方法
CN108913758B (zh) * 2018-07-18 2022-06-14 贝南生物科技（厦门）有限公司 一种用于荧光pcr扩增试剂的冻干方法及其应用
CN111534576B (zh) * 2020-07-08 2020-10-02 圣湘生物科技股份有限公司 用于荧光定量pcr的方法、组合物、试剂盒及其用途

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WO2011120049A1 (fr) * 2010-03-26 2011-09-29 Integrated Dna Technologies, Inc. Procédés pour améliorer l'hybridation des acides nucléiques
WO2016168750A1 (fr) * 2015-04-15 2016-10-20 Biosearch Technologies, Inc. Sondes à désactivateur dual
CN110982876A (zh) * 2020-03-04 2020-04-10 圣湘生物科技股份有限公司 病毒核酸检测的预处理方法、预处理液、试剂盒及其用途

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XIA HONGYAN ET AL: "Development of single-tube nested real-time PCR assays with long internally quenched probes for detection of norovirus genogroup II", BIOTECHNIQUES, vol. 60, no. 1, 2016, US, pages 28 - 34, XP055922158, ISSN: 0736-6205, Retrieved from the Internet <URL:https://www.future-science.com/doi/pdf/10.2144/000114370> DOI: 10.2144/000114370 *

Also Published As

Publication number Publication date
CN111534576B (zh) 2020-10-02
EP4023767A1 (fr) 2022-07-06
EP4023767C0 (fr) 2025-04-02
US20230035871A1 (en) 2023-02-02
CN111534576A (zh) 2020-08-14
WO2022007224A1 (fr) 2022-01-13
BR112022022596A2 (pt) 2023-01-17
EP4023767B1 (fr) 2025-04-02

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