EP4028548A1 - Verfahren zur genotypisierung zur verarbeitung einer vielzahl von proben, insbesondere in fällen von hohem polymorphismus - Google Patents

Verfahren zur genotypisierung zur verarbeitung einer vielzahl von proben, insbesondere in fällen von hohem polymorphismus

Info

Publication number: EP4028548A1
Authority: EP; European Patent Office
Prior art keywords: sequence; primers; sequencing; sequences; universal
Prior art date: 2019-09-12
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Pending

Application number

EP20786024.8A

Other languages

English (en)

French (fr)

Inventor

Pascal Herve

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Bionobis SARL

Original Assignee

Bionobis SARL

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2019-09-12

Filing date

2020-09-11

Publication date

2022-07-20

2020-09-11 Application filed by Bionobis SARL filed Critical Bionobis SARL

2022-07-20 Publication of EP4028548A1 publication Critical patent/EP4028548A1/de

Status Pending legal-status Critical Current

Links

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Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6832—Enhancement of hybridisation reaction
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification

Definitions

low multiplexing conditions are understood to mean conditions making it possible to amplify a single target, or even 2, 3 or 4 targets simultaneously in the same reaction mixture.
Sequencing is carried out using the pair of sequencing primers to amplify the sequences to be analyzed and the index reading primer to identify the patient to which the sequence relates.

Landscapes

Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Health & Medical Sciences (AREA)
Biophysics (AREA)
Chemical Kinetics & Catalysis (AREA)
Immunology (AREA)
Microbiology (AREA)
Molecular Biology (AREA)
Analytical Chemistry (AREA)
Physics & Mathematics (AREA)
Biotechnology (AREA)
Biochemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

EP20786024.8A 2019-09-12 2020-09-11 Verfahren zur genotypisierung zur verarbeitung einer vielzahl von proben, insbesondere in fällen von hohem polymorphismus Pending EP4028548A1 (de)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
FR1910106A FR3100820B1 (fr)	2019-09-12	2019-09-12	Procédé de génotypage adapté au traitement d’un grand nombre d’échantillons, notamment en cas de polymorphisme élevé
PCT/FR2020/051568 WO2021048504A1 (fr)	2019-09-12	2020-09-11	Procédé de génotypage adapté au traitement d'un grand nombre d'échantillons, notamment en cas de polymorphisme élevé

Publications (1)

Publication Number	Publication Date
EP4028548A1 true EP4028548A1 (de)	2022-07-20

Family

ID=69190887

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
EP20786024.8A Pending EP4028548A1 (de)	2019-09-12	2020-09-11	Verfahren zur genotypisierung zur verarbeitung einer vielzahl von proben, insbesondere in fällen von hohem polymorphismus

Country Status (3)

Country	Link
EP (1)	EP4028548A1 (de)
FR (1)	FR3100820B1 (de)
WO (1)	WO2021048504A1 (de)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
FR3110178B1 (fr) *	2020-05-18	2022-05-27	Bionobis	Procédé de génotypage adapté au traitement simultané d’un grand nombre de patients

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
CA2803940C (en)	2010-06-30	2019-07-02	Bgi Genomics Co., Ltd.	Application of a pcr sequencing method, based on dna barcoding technique and dna incomplete shearing strategy, in hla genotyping
US10557134B2 (en) *	2015-02-24	2020-02-11	Trustees Of Boston University	Protection of barcodes during DNA amplification using molecular hairpins

2019
- 2019-09-12 FR FR1910106A patent/FR3100820B1/fr active Active
2020
- 2020-09-11 EP EP20786024.8A patent/EP4028548A1/de active Pending
- 2020-09-11 WO PCT/FR2020/051568 patent/WO2021048504A1/fr not_active Ceased

Also Published As

Publication number	Publication date
FR3100820A1 (fr)	2021-03-19
FR3100820B1 (fr)	2024-06-28
WO2021048504A1 (fr)	2021-03-18

Legal Events

Date	Code	Title	Description
2020-10-14	STAA	Information on the status of an ep patent application or granted ep patent	Free format text: STATUS: UNKNOWN
2021-03-20	STAA	Information on the status of an ep patent application or granted ep patent	Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE
2022-06-17	PUAI	Public reference made under article 153(3) epc to a published international application that has entered the european phase	Free format text: ORIGINAL CODE: 0009012
2022-06-17	STAA	Information on the status of an ep patent application or granted ep patent	Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE
2022-07-20	17P	Request for examination filed	Effective date: 20220322
2022-07-20	AK	Designated contracting states	Kind code of ref document: A1 Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
2022-12-21	DAV	Request for validation of the european patent (deleted)
2022-12-21	DAX	Request for extension of the european patent (deleted)
2024-03-21	STAA	Information on the status of an ep patent application or granted ep patent	Free format text: STATUS: EXAMINATION IS IN PROGRESS
2024-04-24	17Q	First examination report despatched	Effective date: 20240320

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