EP4135732A1 - Traitement microbien pour des systèmes d'eau et l'assainissement du sol - Google Patents

Traitement microbien pour des systèmes d'eau et l'assainissement du sol

Info

Publication number
EP4135732A1
EP4135732A1 EP21788049.1A EP21788049A EP4135732A1 EP 4135732 A1 EP4135732 A1 EP 4135732A1 EP 21788049 A EP21788049 A EP 21788049A EP 4135732 A1 EP4135732 A1 EP 4135732A1
Authority
EP
European Patent Office
Prior art keywords
microbes
sporulated
agent
chemical agent
composition
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP21788049.1A
Other languages
German (de)
English (en)
Other versions
EP4135732A4 (fr
Inventor
Marc Rodriguez
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
NewLife Biosciences LLC
Original Assignee
NewLife Biosciences LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US16/849,823 external-priority patent/US20210276905A1/en
Priority claimed from US17/195,214 external-priority patent/US20210276060A1/en
Application filed by NewLife Biosciences LLC filed Critical NewLife Biosciences LLC
Publication of EP4135732A1 publication Critical patent/EP4135732A1/fr
Publication of EP4135732A4 publication Critical patent/EP4135732A4/fr
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/14—Pretreatment of feeding-stuffs with enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A61K35/742—Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09C—RECLAMATION OF CONTAMINATED SOIL
    • B09C1/00—Reclamation of contaminated soil
    • B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/341—Consortia of bacteria
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
    • B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
    • B09B3/60—Biochemical treatment, e.g. by using enzymes
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/30—Organic compounds
    • C02F2101/306—Pesticides
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2303/00—Specific treatment goals
    • C02F2303/04—Disinfection
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2305/00—Use of specific compounds during water treatment
    • C02F2305/06—Nutrients for stimulating the growth of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/347—Use of yeasts or fungi

Definitions

  • TITLE MICROBIAL TREATMENT FOR WATER SYSTEMS AND SOIL REMEDIATION
  • the present disclosure relates to methods and compositions for water and soil remediation. More specifically, the disclosure relates to methods and compositions for breakdown of contaminants using beneficial microbes.
  • Livestock such as cattle, horses, pigs, sheep, chickens, etc.
  • Systems used to provide drinking water to livestock may be circulating water systems, a water supply to a tank, or a water tank, and can include a holding tank, trough or ball waterer for the livestock to access the drinking water.
  • Contaminants may include algae, bacteria, protozoan parasites, helminth parasites, zoonotic pathogens and/or coliform bacteria. It is important to clean the drinking water to maintain livestock health. Typically cleaning animal drinking water is accomplished by periodic cleaning of watering systems, but this can be labor and time-intensive. For example, a livestock watering tank may need to be regularly drained and scrubbed to maintain a suitable and clean drinking water supply.
  • An object of the present disclosure is to provide a method and composition for cleaning animal drinking water that is effective and simple to carry out.
  • a further object of the present disclosure is to provide a method and composition for breaking down ground contaminants.
  • An object of the present disclosure is to provide a method and composition for breakdown of contaminants using beneficial microbes.
  • a method for treating drinking water for animals including providing a plurality of sporulated microbes to metabolize an organic matter in the drinking water.
  • composition for treating animal drinking water including a plurality of sporulated microbes.
  • a method for breaking down an organic chemical agent comprising providing a plurality of sporulated microbes to the chemical agent.
  • composition for breaking down an organic chemical agent comprising a plurality of sporulated microbes.
  • FIG. l is a flow chart of an embodiment of the system showing an overview of a method of treatment of livestock water, according to one embodiment of the invention.
  • FIGS. 2A and 2B depict tables and graphical representation of results from a microbial treatment of organic chemical agents as an example of the method of the disclosure for an embodiment used for soil remediation.
  • Organic chemical agent means any carbon chain containing chemical, including but not limited to herbicides, insecticides, fungicides, and petroleum products.
  • Merobes as used herein include bacteria, fungi, algae, protozoa, and viruses.
  • a method and composition is provided in the present disclosure for cleaning animal drinking water or maintaining clean animal drinking water, by treatment with sporulated microbes.
  • the sporulated microbes provide a stable format that is temperature and pressure resistant. Upon exposure to the drinking water, the sporulated bacteria are released and activated, and metabolize organic matter in the drinking water, thus cleaning the drinking water.
  • the method 10 comprises providing animal drinking water 12 in a container. Sporulated microbes are introduced 14 into the animal drinking water. In one embodiment, the microbes are released 16 and activated 18. The microbes metabolize 20 organic matter in the water thus cleaning 22 the drinking water.
  • Sporulated microbes form in response to inhospitable conditions, such as a severe lack of nutrients, that would be lethal for the normal (vegetative) form of the microbe.
  • the bacteria respond by producing and activating a protein that promotes the transcription of genes required for the conversion of the actively growing bacterium to a spore.
  • the bacterial genome is duplicated and the second copy of the genome is enveloped together with some cytoplasm within an in-growth of the bacterial cell well.
  • the resultant daughter cell is stabilized by formation of another membrane layer, a peptidoglycan material between the two membrane layers, and an outer coat of proteins, thereby forming an almost impregnable sphere.
  • Bacteria are induced to be in the sporulated state by culture at high density (for example at 1.0 x 10 11 CFU/Gallon) in a media lacking nutrients, such as Tryptone, Soy and Proteins.
  • the sporulated bacteria can be activated to come out of the dormant state in the presence of appropriate nutrients for the sporulated bacteria and/or in the presence of a higher pH.
  • agents can be added with the microbes.
  • agents include an anti-pathogen agent to mitigate pathogens, such as protozoan parasites, helminth parasites, zoonotic pathogens and coliform bacteria.
  • Additional agents include an animal digestive system enhancement agent (for example bacteria for digesting cellulose and lignan), an animal microbiome stability agent, an animal immune system support agent and a water contaminant mitigation agent. These additional agents are provided to the drinking water together with the sporulated microbes.
  • Water contamination mitigation agents may include nitrifying bacteria for oxidizing ammonia to nitrites and nitrites to nitrates.
  • Water treatment using microbes can incorporate cleaning bacteria and nitrifying bacteria.
  • Cleaning bacteria digest decomposing organic material, which produces ammonia, and assists nitrifying bacteria by preventing loss of oxygen caused by the decomposing material.
  • Nitrifying bacteria include ammonia oxidizing bacteria that convert ammonia into nitrites, and nitrite oxidizing bacteria that convert nitrite into nitrate.
  • Water contamination mitigation agents may also include chelating agents for chelating metals and metalloids, such as arsenic and aluminum.
  • Other water contamination mitigation agents are bacteria for metabolizing pesticides or herbicides, such as glyphosate, Dicamba, 2,4-dichlorophenoxyacetic acid, Atrazine, Fomesafen, Imidacloprid, Clothianidin or Thiamethoxam.
  • the sporulated microbes may be provided in a format such as a pellet, a tablet, a granule, a bolus, a spike, a mash, a crumble, a meal, a liquid, or a powder.
  • the sporulated microbes are at least 1 x 10 11 CFU/Gallon and a pH lower than 8.5, which maintains the sporulated bacteria in a dormant state.
  • the sporulated microbes are at least two of Bacillus amyloliquefaciens, Bacillus subtilis, Rhodopseudomonas palustris, and Saccharomyces cerevisiae.
  • the sporulated microbes are adsorbed or packed with a carrier or provided in a format such as a tablet.
  • the packing or carrier affects the rate of release of the microbes by providing a level of water accessibility or effervescence. With greater water accessibility or effervescence of the carrier, the microbes are released faster, and with lower water accessibility or effervescence, the release is slower and can be extended to at least 24 hours.
  • the release rate can determine the CFU count in the treated drinking water, so different carriers can be utilized for different CFU concentration in the drinking water and different dosing characteristics.
  • sporulated microbes In addition, faster release tends to enable a greater concentration of the sporulated microbes and other additional agents towards the top of the water column of the drinking water, whereas slower release tends to enable a greater concentration of the sporulated microbes and additional agents towards the bottom of the water column of the drinking water.
  • Slower release of the sporulated microbes also has an advantage of extending the water treatment time and requiring fewer additions of the treatment.
  • carriers are salts and inert minerals such as zeolite and calcium byproducts.
  • a ratio of aerobic to anaerobic microbes is adjusted to target the condition of the water to be treated and attain a minimum CFU/Gallon of microbes in the drinking water.
  • the ratio can be adjusted to take into account the water depth and level in the water column that is being targeted for treatment. Aerobic microbes will inhabit areas closer to the water surface, while anaerobic microbes will inhabit areas at greater water depth. Thus by providing a higher aerobic to anaerobic microbe ratio, a larger proportion of the microbes will be concentrated higher in the water column. Conversely, a lower aerobic to anaerobic microbes ratio will result in a larger proportion of the microbes lower in the water column.
  • the anaerobic microbes are more effective in reducing organic matter.
  • the aerobic microbes are more effective in treating adverse chemical conditions of the drinking water and are advantageous for animals to drink, for example to reduce coliforms. Thus the ratio of aerobic to anaerobic microbes can be customized to the water treatment required.
  • a composition for treating animal drinking water includes sporulated microbes as described above.
  • the composition may further include an anti-pathogen agent, an animal digestive system enhancement agent, an animal microbiome stability agent, an animal immune system support agent, and/or a water contamination mitigation agent.
  • a water contamination mitigation agent are a nitrifying bacteria, a chelating agent for a metal or a metalloid, and a bacteria for metabolizing a pesticide.
  • the composition for treating animal drinking water may be in the form of a pellet, tablet, granule, bolus, spike, mash, crumble, meal, liquid or powder.
  • the liquid may preferentially be a solution with a concentration of the sporulated microbes of at least 1 x 10 11 CFU/gallon and a pH lower than 8.5.
  • Sporulated microbes that may be included in the composition are at least two of
  • the composition may also include a carrier for the sporulated microbes.
  • the characteristics of the carrier may be used to regulate the release rate of the microbes into the drinking water.
  • the microbe composition may also be varied in the ratio of aerobic to anaerobic microbes, which would vary the distribution of the microbes once released into the drinking water.
  • An object of an alternative embodiment discussed herein is to provide a system and method for breakdown of agricultural products using beneficial microbes.
  • Organic chemical agents can be broken down by microbes, including a mixture of beneficial bacteria.
  • the mixture of beneficial bacteria can include a first set of bacteria that break down the chemical agent and a further set of bacteria that, for example, break down the products of the first set of bacteria.
  • This mixture of a plurality of bacteria may be referred to as a consortium of bacteria because the bacterial mixture works together more effectively than if it were added in parts.
  • the effectiveness or efficacy of the bacterial consortium refers to the effectiveness of the bacterial consortium at breaking down the organic chemical agent.
  • the efficacy of the organic chemical agent refers to the effectiveness of the organic chemical agent at treating its target; for example, the efficacy of a herbicide at eliminating weeds.
  • the bacteria are sporulated to provide a stable format that is resistant to damage and is not active until distribution, for example, distribution on plants or soil.
  • Organic chemical agents may be an agricultural agent, a petroleum agent, an industrial site agent, or a metabolite or breakdown product of the chemical agent.
  • Agricultural agents are, for example, herbicides, insecticides, or fungicides.
  • Herbicides may include glyphosate, dicamba, 2,4-dichlorophenoxyacetic acid (also called 2,4-D), atrazine, fomesafen, triazine, or sulfonylurea herbicides; and insecticides may include neonicotinoids such as imidacloprid, clothianidin, and thiamethoxam.
  • Organic chemical agents may occur for example, in agricultural settings, railroad right of ways, laneways, home and garden settings, golf courses, sign boulevards, road shoulders, powerline greenways, gas line right of ways, and water sources.
  • the treatment of a chemical agent may be done by adding the mixture of sporulated microbes to the organic chemical agent prior to distribution on plants or soil.
  • the sporulated bacteria do not degrade the organic chemical agent prior to distribution because they remain in the dormant state.
  • the sporulated bacteria remain dormant in the presence of the chemical agent until approximately 24 hours after the distribution of the mixture onto, for example, plants, such as agricultural crops or onto the soil.
  • the lag time of approximately 24 hours for the sporulated bacteria to become active allows the chemical agent, which is typically active within one hour of application, to achieve its function, for example, as a herbicide or insecticide, before being degraded by the beneficial bacteria regenerated from the sporulated bacteria.
  • the efficacy of the bacterial consortium at breaking down the chemical agent after activation is retained in the mixture because the bacteria is sporulated and thus stable.
  • the efficacy of the chemical agent is retained in the mixture because while in the mixture, the bacteria remains sporulated and inactive and thus does not break down the chemical agent.
  • the treatment of a chemical agent can be done by distributing the microbe mixture onto plants after the plants have been treated with the chemical agent.
  • an agricultural crop that is resistant to the chemical agent may be treated with the chemical agent, followed by a distribution of the microbe mixture to remove residual chemical agent or the metabolites of the chemical agent.
  • a further method is to treat the chemical agent by distributing the microbe mixture on soil that has been treated with the chemical agent, or on soil that is contaminated with the chemical agent.
  • the sporulated microbes may be distributed on sidewalks, parks, residential lawns and driveways, sports fields, golf courses, or in water sources, such as water wells, wherein the site of distribution is a non-intended area for the chemical agent.
  • the sporulated microbes may be mixed with an agent, such as a drying agent, for spill cleanup to distribute on a chemical agent spill.
  • a drying agent such as cat litter, com cobs, and foam spray.
  • the sporulated microbes may be impregnated into the drying agent in the packaging of the mixture before distribution of the mixture over the chemical agent spill.
  • the sporulated microbes may be in a pellet, powder, liquid, or granule format.
  • the format allows a high concentration of the sporulated microbes that is sufficient to break down the chemical agent.
  • the sporulated microbes are provided at a high enough concentration to break down the chemical agent on a plant crop, resulting in a reduction of the active form of the chemical agent in the plant food product.
  • the sporulated microbes are provided at a concentration that allows replanting of a crop in the treatment area after a set time, without risk of injury to the crop by the chemical agent or by the microbe mixture.
  • the set time is shorter than if there was no treatment with the sporulated microbes.
  • the concentration of microbes may allow replanting a crop one month after treatment with the sporulated microbe mixture.
  • the concentration of sporulated microbes may be determined by the amount of chemical agent that needs to be broken down, the type of chemical agent to be broken down, and the soil type that is being treated.
  • the sporulated microbes may be in a solution with a concentration of at least 1 x 10 11 CFU/Gallon.
  • the sporulated microbes may be in a liquid format, at a pH lower than 8.5 or ideally at a pH between 4.5 and 5.5. In this format, the sporulated microbes do not degrade the organic chemical. Thus they can be mixed with the organic chemical agent prior to activation of the sporulated microbes without degradation of the organic chemical. This provides a useful format to package an organic chemical, such as a herbicide or insecticide, together with the dormant sporulated microbes, prior to application, for example, to agricultural crops.
  • an organic chemical such as a herbicide or insecticide
  • the activated bacteria degrade the chemical agent by breaking down the carbon chain of the chemical agent or other covalent bonds that are metabolic targets of the active bacteria. Breakdown of the chemical bonds of the chemical agent may be carried out by enzymes secreted by the active bacteria.
  • a mixture of at least two bacterial species is used to provide a broader spectrum of active proteins and enzymes to metabolize the organic chemical agent.
  • the bacterial mixture also works synergistically such that one bacterial species may degrade metabolic products created by another bacterial species.
  • enzymes secreted by one bacterial species may target degradation products of the organic chemical agent.
  • the combination of bacterial species provides greater activity than a simple additive advantage of individual species.
  • the sporulated microbes may be gram-negative and gram-positive bacteria that metabolize or break down a carbon bond or a covalent bond.
  • the sporulated bacteria may be motile bacteria that metabolize or break down a carbon bond or a covalent bond. Further the sporulated bacteria may be Bacillus bacteria that metabolize or break down a carbon bond or a covalent bond.
  • the sporulated microbes may be a mixture of at least two of the following bacterial species: Bacillus licheniformis; Bacillus coagulans; Bacillus subtilis; Bacillus pumilus; Bacillus megaterium; and Bacillus amyloliquefaciens.
  • cellulose- and lignan- composting bacteria may be included in the microbe mixture to break down crop waste such as dying material after a crop has been harvested and/or to break down weed waste.
  • the format provides an opportunity to premix other products into the formulation to provide an optimized formulation.
  • An optimized sporulated microbe formulation includes the sporulated microbes and other ingredients that benefit the microbial activity, such as a nutrient, an extract, a sugar, or an activating agent of the sporulated microbes to provide a food source for the microbes and improve microbial performance.
  • An example embodiment of the present disclosure is provided below, including customer directions for a biological soil amendment for bio-remediation of pesticides and petroleum spills and residues.
  • the product consists of a concentrated microbial powder which is a highly effective and concentrated biological amendment containing a broad spectrum of microbials specially selected for pesticide and petroleum remediation.
  • the concentrated package of bacteria can be sprayed with water or mixed with all fertilizer types, bio-stimulants, or adjuvants to effectively and efficiently remove pesticide and petroleum residues and spills from soils.
  • Directions for use of the powder for irrigation applications mix the powder at well site in container to allow water soluble bags to dissolve and be mixed thoroughly before pumping through irrigation system. Can be applied with drip or foliar irrigation equipment. Do not apply with flood in-furrow irrigation. Can be applied in conjunction with all fertilizers or micronutrients formulations.
  • the powder includes 75.50% inert ingredients and 24.5% of non-plant food ingredients, including the following soil health microbes:
  • the powder has a five-year shelf life if kept dry and does not require refrigeration. The end user is to follow SDS instructions for safety precautions, cleaning and PPE (Personal Protective Equipment). If no such instructions for washables, use detergent and hot water.
  • FIGS. 2A and 2B An example of the treatment of a variety of herbicides and insecticides is presented in FIGS. 2A and 2B.
  • Chemical treatments including the herbicides: glyphosate; Dicamba; 2,4-Dichlorophenoxyacetic acid (also called 2,4-D); Atrazine; and Fomesafen herbicide; and the insecticides: Imidacloprid; Clothianidin; and Thiamethoxam, were applied to the soil at an undiluted concentration (100% ) or diluted as recommended by the manufacturer (Application Field Rate). After the soil was treated and dry (approximately 45 minutes after chemical treatment), the sporulated microbes were added. Samples were taken 45 days following treatment and analyzed for residual chemical. For each chemical and for both the undiluted chemical and the diluted chemical, the microbes are able to reduce the level of the organic chemical by at least 50% in 45 days.
  • a composition for the treatment of an organic chemical agent is made up of a consortium of sporulated microbes.
  • the composition may be a mixture of sporulated microbes and the organic chemical agent to be treated, both of which retain their efficacy when combined in the mixture.
  • the mixture of sporulated microbes may be a solution with a density of at least 1 x 10 11 CFU/Gallon.
  • the microbes are dormant in the composition, but able to be activated when the pH rises above 5.5 and/or when the bacterial spores are exposed to nutrients.
  • the microbes in the composition may contain at least two of: Bacillus licheniformis; Bacillus coagulans; Bacillus subtilis; Bacillus pumilus; Bacillus megaterium; and Bacillus amyloliquefaciens.
  • the composition may include cellulose- and lignan- composting bacteria in the microbe mixture to break down dying material after a crop has been harvested and/or to break down weed waste.
  • the composition may also include a nutrient, an extract, or a sugar to improve microbial performance.
  • a nutrient such as glucose, glycerol, or a sugar to improve microbial performance.
  • An example would be to add seaweed extract.

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Polymers & Plastics (AREA)
  • Mycology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Physiology (AREA)
  • Animal Husbandry (AREA)
  • Food Science & Technology (AREA)
  • Environmental & Geological Engineering (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Hydrology & Water Resources (AREA)
  • Water Supply & Treatment (AREA)
  • Organic Chemistry (AREA)
  • Soil Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Procédé et composition destinés à décomposer un contaminant. Un procédé destiné à décomposer un contaminant dans de l'eau potable pour animaux ou un agent chimique organique comprend la fourniture d'une pluralité de microbes sporulés. Une composition destinée à décomposer un contaminant dans de l'eau potable pour animaux ou un agent chimique organique comprend une pluralité de microbes sporulés.
EP21788049.1A 2020-04-15 2021-04-15 Traitement microbien pour des systèmes d'eau et l'assainissement du sol Pending EP4135732A4 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US16/849,823 US20210276905A1 (en) 2020-03-06 2020-04-15 Microbial treatment for livestock water systems
US17/195,214 US20210276060A1 (en) 2020-03-06 2021-03-08 Soil remediation through beneficial microbes
PCT/IB2021/053110 WO2021209942A1 (fr) 2020-04-15 2021-04-15 Traitement microbien pour des systèmes d'eau et l'assainissement du sol

Publications (2)

Publication Number Publication Date
EP4135732A1 true EP4135732A1 (fr) 2023-02-22
EP4135732A4 EP4135732A4 (fr) 2024-05-08

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EP (1) EP4135732A4 (fr)
AU (1) AU2021254883A1 (fr)
CA (1) CA3175693A1 (fr)
GB (1) GB2611433A (fr)
WO (1) WO2021209942A1 (fr)

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CN119020040B (zh) * 2024-08-16 2025-03-21 中国科学院南京土壤研究所 含铁纳米材料在消减氟磺胺草醚残留的应用及消减方法

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